Mus musculus (mm10), chr6:52313498-52314271, forward genomic strand
Your input sequence is 774 bp long. It contains 161 possible guide sequences.
Shown below are their PAM sites and the expected cleavage position located -3bp 5' of the PAM site.
Click on a match for the PAM NGG below to show its 20 bp-long guide sequence. (Need help? Look at the CRISPOR manual)
Colors green, yellow and red indicate high, medium and low specificity of the PAM's guide sequence in the genome.

Variant database: Missing a variant database? We can add it.
Position  0         10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       
Variants  ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
Sequence  ctcctttcatcttcactgtggcagacgtttctatttatccacttgcgttcgccgagtggcgtcaccagcggtactgtaatgacgattgcagcaggaggatgacagcttagaaagaagagggcaatggggcttcctcccagaggcggtgcggcacagaggagcgctcgcatcacaaggtgaccctagctccccactgccatctccgcggtcgccgtcgacacggcgctggggctacccggcgcctgccttgtcgccttagctcctcttctcagccaagatcccagggagcctgggattaggagcttacttgggggttttccccctccccccctctggagagtccggggATGgagagccgaaaggacatggttatgtttctggatgggggtcagcttggcactctggttggtaagagggtctctaatttgtccgaagccgtgagcagcccgctgcctgaaccgccagagaagatggtgccccacggttgcctgagcccgcgagccggccctccgacttcccgggagcgtggcgggggaggccaggaggaggagccggtcgatggactagcaggcagtgctgcagggctgggcgccgagccacggtctgctggagcggccatgcttggcccgggacccccagtcccctccgcggacagcctctctggccaagggcaacctagtagctcagacaccgaatcggatttctatgaagaaatcgaggtgagctgcaccccagactgcgccaccgggaacgccgagtaccagcacagcaaag
            CCT---                              CCA---       CCG---       CCA---                   −−−AGG                   −−−AGG  −−−GGG    CCT---  −−−CGG       −−−AGG            −−−AGG   CCC---  CCC---  CCA---         CCG---      −−−TGG     CCC---     CCT---  CCT---  CCT---     CCA---  CCA---  CCT---           −−−TGG       CCC---  CCC---         CCG---        CCG---  −−−TGG      −−−TGG          −−−TGG  −−−TGG     −−−AGG             CCG---          CCC---       CCG---    −−−TGG  CCC---     CCT---        CCG---  CCG---  CCG---   −−−CGG      CCA---       CCG---        −−−AGG      −−−AGG        CCG---       −−−TGG     CCA---    CCC---  CCC---  CCC---        CCT---   CCA---    CCT---          CCG---                  −−−AGG         CCC---      CCA---
                                                               −−−TGG      −−−CGG                     −−−AGG                 −−−GGG  −−−GGG      CCC---    −−−CGG                              CCT---  CCC---       CCG---         −−−CGG  −−−GGG    CCG---                                      CCC---  −−−TGG             −−−GGG       CCC---  CCC---      −−−CGG            −−−AGG                −−−TGG                  −−−TGG  −−−GGG                  CCG---     CCG---         CCA---          CCC---          CCC---      CCC---     CCC---     −−−GGG    −−−AGG      −−−CGG                       −−−GGG            CCA---       −−−CGG    −−−TGG       CCC---  CCC---            −−−TGG                             −−−CGG                               CCC---        CCG---
                                                                                                                                   −−−TGG         CCA---                                                CCA---      −−−CGG                −−−GGG   −−−CGG  CCT---                                −−−AGG   −−−GGG             −−−GGG       CCC---  CCC---      −−−GGG                                  −−−GGG                                                                  CCT---                    CCA---          CCG---      CCT---   −−−CGG  −−−TGG   −−−AGG  −−−AGG          −−−TGG                    −−−TGG        −−−CGG                        CCG---  CCC---  CCT---                −−−AGG                                                             CCA---     −−−CGG
                                                                                                                                                   −−−AGG                                                                                                                                         −−−GGG        −−−AGG        −−−GGG       CCT---  CCT---      −−−GGG                                  −−−GGG                                                                                           −−−CGG               −−−CGG           −−−GGG      −−−GGG         −−−AGG                                  −−−GGG                                   −−−CGG     CCA---    CCG---              −−−GGG                                                                        −−−GGG
                                                                                                                                                                                                                                                                                                                                              CCC---               −−−TGG                               −−−GGG                                                                                                                                             −−−GGG                                                                                          −−−GGG              −−−CGG
                                                                                                                                                                                                                                                                                                                                               CCC---
                                                                                                                                                                                                                                                                                                                                                    −−−TGG

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Predicted guide sequences for PAMs
Ranked by default from highest to lowest specificity score (Hsu et al., Nat Biot 2013). Click on a column title to rank by a score.
If you use this website, please cite our paper in NAR 2018. Too much information? Look at the CRISPOR manual.

Download as Excel tables: Guides /  Guides, all scores /  Off-targets /  Saturating mutagenesis assistant
Position/
Strand
Guide Sequence + PAM
+ Restriction Enzymes
+ Variants
Only G- Only GG- Only A-
MIT Specificity Score CFD Spec. score Predicted Efficiency
Show main scores
Outcome Off-targets for
0-1-2-3-4 mismatches
+ next to PAM
Genome Browser links to matches sorted by CFD off-target score
exons only chr6 only
39 / rev CGCCACTCGGCGAACGCAAG TGG
.................... ...
 Cloning / PCR primers
99 99 48 50 47 0.0 63 5 36 52 43 73 72 80 0 - 0 - 0 - 0 - 4
0 - 0 - 0 - 0 - 0

4 off-targets
show all...
57 / fw ATCCACTTGCGTTCGCCGAG TGG
.................... ...
Enzymes: NmeAIII, AcyI
 Cloning / PCR primers
99 99 61 57 62 0.2 70 45 33 33 45 67 81 87 0 - 0 - 0 - 1 - 7
0 - 0 - 0 - 0 - 0

8 off-targets
show all...
221 / fw CTCCGCGGTCGCCGTCGACA CGG
.................... ...
Enzymes: BfoI, HinP1I, Hpy166II, TaqI, BceAI, HincII, SalI, FblI
 Cloning / PCR primers
99 98 47 46 50 -0.0 0 13 33 42 39 65 64 76 0 - 0 - 0 - 1 - 14
0 - 0 - 0 - 0 - 0

15 off-targets
show all...
65 / rev AATCGTCATTACAGTACCGC TGG
.................... ...
Enzymes: AsuHPI, MspA1I, SsiI, MaeIII, Tsp45I
 Cloning / PCR primers
98 98 57 59 62 0.2 0 14 73 28 14 67 57 74 0 - 0 - 0 - 0 - 20
0 - 0 - 0 - 0 - 0

20 off-targets
show all...
212 / rev TAGCCCCAGCGCCGTGTCGA CGG
.................... ...
Enzymes: BceAI, Hpy166II, Hpy99I, HincII, TaqI, SalI, FblI
 Cloning / PCR primers
97 97 48 51 79 0.1 0 13 57 59 65 63 52 84 0 - 0 - 0 - 1 - 27
0 - 0 - 0 - 0 - 0

28 off-targets
show all...
228 / fw GTCGCCGTCGACACGGCGCT GGG
.................... ...
Enzymes: HinP1I, BfoI
 Cloning / PCR primers
97 98 39 35 60 -0.1 0 8 46 53 56 63 60 88 0 - 0 - 0 - 1 - 11
0 - 0 - 0 - 0 - 0

12 off-targets
show all...
229 / fw TCGCCGTCGACACGGCGCTG GGG
.................... ...
Enzymes: HinP1I, BfoI
 Cloning / PCR primers
97 99 63 66 92 0.6 0 24 75 70 64 73 65 85 0 - 0 - 0 - 1 - 14
0 - 0 - 0 - 1 - 0

15 off-targets
show all...
732 / rev GCGTTCCCGGTGGCGCAGTC TGG
.................... ...
 Cloning / PCR primers
97 99 26 22 37 -0.8 0 2 36 64 42 68 60 82 0 - 0 - 0 - 1 - 19
0 - 0 - 0 - 0 - 0

20 off-targets
show all...
52 / rev GTACCGCTGGTGACGCCACT CGG
.................... ...
 Cloning / PCR primers
96 97 56 59 73 -0.1 0 75 55 45 53 72 81 88 0 - 0 - 0 - 2 - 25
0 - 0 - 0 - 0 - 0

27 off-targets
show all...
203 / rev CGCCGTGTCGACGGCGACCG CGG
.................... ...
High GC content
Enzymes: BstDSI, SacII, BspFNI, MspA1I, BseDI, Bsh1285I, SsiI
 Cloning / PCR primers
96 99 52 47 76 0.3 0 52 53 67 60 74 51 66 0 - 0 - 0 - 1 - 16
0 - 0 - 0 - 0 - 0

17 off-targets
show all...
227 / fw GGTCGCCGTCGACACGGCGC TGG
.................... ...
High GC content
Enzymes: HinP1I, BceAI, BfoI
 Cloning / PCR primers
96 98 30 31 77 0.3 0 7 82 24 22 83 60 71 0 - 0 - 0 - 2 - 14
0 - 0 - 0 - 0 - 0

16 off-targets
show all...
478 / rev TCGCGGGCTCAGGCAACCGT GGG
.................... ...
Enzymes: BstDSI, BaeGI, NlaIV, BseDI, BanI, Bsp1286I, HpyCH4III
 Cloning / PCR primers
96 98 46 45 29 0.9 0 10 78 74 61 73 68 85 0 - 0 - 0 - 1 - 23
0 - 0 - 0 - 0 - 0

24 off-targets
show all...
742 / rev CTGGTACTCGGCGTTCCCGG TGG
.................... ...
Enzymes: MspI, HinP1I, LpnPI, StyD4I, NciI
 Cloning / PCR primers
96 97 65 66 88 1.0 82 29 86 74 60 81 52 64 0 - 0 - 0 - 2 - 19
0 - 0 - 0 - 0 - 0

21 off-targets
show all...
69 / fw TCGCCGAGTGGCGTCACCAG CGG
.................... ...
Enzymes: Csp6I, LpnPI, MspA1I, MwoI
 Cloning / PCR primers
95 98 61 60 97 0.8 82 49 80 98 76 78 57 54 0 - 0 - 0 - 1 - 20
0 - 0 - 0 - 1 - 0

21 off-targets
show all...
569 / fw GAGCCGGTCGATGGACTAGC AGG
.................... ...
Enzymes: BstC8I, BtsIMutI, BtsI, MaeI
 Cloning / PCR primers
95 96 51 48 65 0.0 0 28 55 67 68 50 62 79 0 - 0 - 0 - 3 - 21
0 - 0 - 0 - 0 - 0

24 off-targets
show all...
731 / rev CGTTCCCGGTGGCGCAGTCT GGG
.................... ...
Enzymes: HpyCH4V
 Cloning / PCR primers
95 98 34 36 26 -0.3 0 0 27 60 35 65 61 65 0 - 0 - 1 - 4 - 22
0 - 0 - 0 - 2 - 0

27 off-targets
show all...
745 / rev GTGCTGGTACTCGGCGTTCC CGG
.................... ...
Inefficient
Enzymes: MspI, NciI, LpnPI, StyD4I
 Cloning / PCR primers
95 97 39 34 16 -0.8 0 3 20 72 65 68 55 72 0 - 0 - 0 - 0 - 54
0 - 0 - 0 - 0 - 0

54 off-targets
show all...
93 / fw ACTGTAATGACGATTGCAGC AGG
.................... ...
Enzymes: EcoP15I, BstV1I, ApeKI, Fsp4HI, HpyCH4V
 Cloning / PCR primers
94 96 57 54 37 0.4 0 18 60 35 31 73 70 79 0 - 0 - 0 - 2 - 44
0 - 0 - 0 - 1 - 0

46 off-targets
show all...
494 / rev AAGTCGGAGGGCCGGCTCGC GGG
.................... ...
Enzymes: BspFNI, BspCNI, BstDEI, BslI, Bsp1286I, Eco24I, BstC8I
 Cloning / PCR primers
94 97 42 38 91 0.1 0 3 62 61 47 59 70 85 0 - 0 - 0 - 2 - 19
0 - 0 - 0 - 0 - 0

21 off-targets
show all...
697 / fw TAGTAGCTCAGACACCGAAT CGG
.................... ...
Enzymes: HinfI, Hpy188I, PfeI
 Cloning / PCR primers
94 95 63 57 52 0.1 0 52 66 57 42 61 55 72 0 - 0 - 0 - 5 - 54
0 - 0 - 0 - 0 - 0

59 off-targets
show all...
479 / rev CTCGCGGGCTCAGGCAACCG TGG
.................... ...
Enzymes: Bsp1286I, BstDSI, BaeGI, HpyCH4III, BseDI
 Cloning / PCR primers
93 97 52 49 96 0.6 64 16 79 54 62 74 67 82 0 - 0 - 0 - 1 - 42
0 - 0 - 0 - 0 - 0

43 off-targets
show all...
503 / fw GGTTGCCTGAGCCCGCGAGC CGG
.................... ...
Enzymes: BshFI, MnlI, MspI, PspPI, LpnPI, BslI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
93 95 53 48 71 0.5 0 14 70 39 39 69 67 82 0 - 0 - 0 - 3 - 53
0 - 0 - 0 - 0 - 0

56 off-targets
show all...
532 / fw GACTTCCCGGGAGCGTGGCG GGG
.................... ...
Enzymes: MwoI
 Cloning / PCR primers
93 95 44 42 88 0.4 0 23 78 55 55 75 65 85 0 - 0 - 0 - 3 - 47
0 - 0 - 0 - 0 - 1

50 off-targets
show all...
552 / rev CTGCCTGCTAGTCCATCGAC CGG
.................... ...
Enzymes: NlaIV, MspI, BslI, TaqI, LpnPI, Bsh1285I, LmnI, Cfr10I
 Cloning / PCR primers
93 94 69 62 6 0.5 0 29 41 58 44 53 72 79 0 - 0 - 0 - 4 - 61
0 - 0 - 0 - 0 - 0

65 off-targets
show all...
190 / rev GCGACCGCGGAGATGGCAGT GGG
.................... ...
Enzymes: AluBI, BtsIMutI, BtsI, TspRI
 Cloning / PCR primers
92 97 56 60 60 0.2 0 30 69 64 60 61 63 75 0 - 0 - 0 - 3 - 34
0 - 0 - 0 - 1 - 0

37 off-targets
show all...
197 / rev GTCGACGGCGACCGCGGAGA TGG
.................... ...
Enzymes: MwoI, BtsIMutI, BtsI
 Cloning / PCR primers
92 97 51 51 65 0.1 0 10 70 70 59 57 48 60 0 - 0 - 0 - 4 - 31
0 - 0 - 0 - 1 - 1

35 off-targets
show all...
477 / rev CGCGGGCTCAGGCAACCGTG GGG
.................... ...
Enzymes: BstDSI, BaeGI, NlaIV, BseDI, BanI, Bsp1286I, HpyCH4III
 Cloning / PCR primers
92 96 64 63 91 0.7 0 47 68 79 75 75 74 88 0 - 0 - 1 - 4 - 42
0 - 0 - 0 - 0 - 0

47 off-targets
show all...
506 / rev CACGCTCCCGGGAAGTCGGA GGG
.................... ...
Enzymes: BshFI, MspI, Hpy188I, PspPI, LpnPI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
92 95 54 54 75 0.4 63 54 61 56 51 67 65 78 0 - 0 - 0 - 6 - 33
0 - 0 - 0 - 0 - 0

39 off-targets
show all...
510 / rev CCGCCACGCTCCCGGGAAGT CGG
.................... ...
Enzymes: Hpy188I
 Cloning / PCR primers
92 97 43 53 54 -0.2 0 6 27 74 48 57 68 82 0 - 0 - 0 - 1 - 41
0 - 0 - 0 - 0 - 1

42 off-targets
show all...
597 / rev ATGGCCGCTCCAGCAGACCG TGG
.................... ...
Enzymes: BstDSI, BslI, HpyCH4III, BseDI
 Cloning / PCR primers
92 96 63 66 80 1.1 72 45 87 49 54 71 66 84 0 - 0 - 1 - 0 - 42
0 - 0 - 0 - 0 - 2

43 off-targets
show all...
495 / rev GAAGTCGGAGGGCCGGCTCG CGG
.................... ...
Enzymes: BspFNI, Bsp1286I, BslI, Eco24I, BstC8I
 Cloning / PCR primers
91 94 49 42 94 0.4 52 5 77 54 47 90 71 80 0 - 0 - 0 - 4 - 47
0 - 0 - 0 - 0 - 0

51 off-targets
show all...
531 / fw CGACTTCCCGGGAGCGTGGC GGG
.................... ...
 Cloning / PCR primers
91 96 34 30 28 -0.5 0 2 27 43 46 70 64 91 0 - 0 - 0 - 3 - 37
0 - 0 - 0 - 0 - 0

40 off-targets
show all...
533 / fw ACTTCCCGGGAGCGTGGCGG GGG
.................... ...
Enzymes: BshFI, MwoI
 Cloning / PCR primers
91 94 46 46 93 0.8 0 16 85 81 60 72 62 79 0 - 0 - 0 - 3 - 71
0 - 0 - 0 - 0 - 1

74 off-targets
show all...
642 / rev CCAGAGAGGCTGTCCGCGGA GGG
.................... ...
Enzymes: BstDSI, BslFI, SacII, BspFNI, MspA1I, BseDI, BslI
 Cloning / PCR primers
91 96 47 50 60 0.2 0 6 69 63 58 71 68 76 0 - 0 - 0 - 5 - 87
0 - 0 - 0 - 0 - 0

92 off-targets
show all...
133 / rev TGTGCCGCACCGCCTCTGGG AGG
.................... ...
Enzymes: BstENI, BslI
 Cloning / PCR primers
90 94 62 65 93 0.4 73 51 79 51 39 80 67 83 0 - 0 - 0 - 8 - 48
0 - 0 - 0 - 2 - 1

56 off-targets
show all...
430 / rev CAGCGGGCTGCTCACGGCTT CGG
.................... ...
Inefficient
Enzymes: BceAI, Hpy188I
 Cloning / PCR primers
90 95 42 36 3 0.1 0 5 41 80 50 78 61 67 0 - 0 - 1 - 4 - 54
0 - 0 - 0 - 0 - 0

59 off-targets
show all...
519 / fw GAGCCGGCCCTCCGACTTCC CGG
.................... ...
Inefficient
Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, Cfr9I
 Cloning / PCR primers
90 92 39 32 36 -0.6 0 3 43 51 31 57 77 85 0 - 0 - 1 - 5 - 67
0 - 0 - 0 - 0 - 0

73 off-targets
show all...
530 / fw CCGACTTCCCGGGAGCGTGG CGG
.................... ...
 Cloning / PCR primers
90 92 56 52 73 0.5 0 26 74 66 53 73 67 85 0 - 0 - 0 - 3 - 54
0 - 0 - 0 - 0 - 2

57 off-targets
show all...
643 / rev GCCAGAGAGGCTGTCCGCGG AGG
.................... ...
Enzymes: BstDSI, BslFI, SacII, BspFNI, MspA1I, BseDI, BslI, SsiI
 Cloning / PCR primers
90 91 47 55 96 1.0 0 5 90 79 72 75 68 81 0 - 0 - 1 - 8 - 77
0 - 0 - 0 - 0 - 0

86 off-targets
show all...
691 / rev TTCTTCATAGAAATCCGATT CGG
.................... ...
Enzymes: HinfI, Hpy188I, PfeI
 Cloning / PCR primers
90 94 45 50 25 -0.3 0 25 26 43 37 61 54 81 0 - 0 - 1 - 8 - 90
0 - 0 - 0 - 1 - 0

99 off-targets
show all...
746 / fw TGCACCCCAGACTGCGCCAC CGG
.................... ...
Enzymes: MspI, NciI, LpnPI, StyD4I
 Cloning / PCR primers
90 97 44 43 34 -0.2 0 2 33 63 48 54 57 63 0 - 0 - 1 - 4 - 88
0 - 0 - 0 - 0 - 0

93 off-targets
show all...
137 / rev CCTCTGTGCCGCACCGCCTC TGG
.................... ...
Enzymes: BstENI, BslI, MnlI
 Cloning / PCR primers
89 96 30 25 34 -0.3 0 1 28 49 34 81 71 72 0 - 0 - 0 - 1 - 107
0 - 0 - 0 - 0 - 0

108 off-targets
show all...
236 / rev TAAGGCGACAAGGCAGGCGC CGG
.................... ...
Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I, BstC8I
 Cloning / PCR primers
89 92 44 48 37 1.1 0 7 88 47 40 74 63 72 0 - 0 - 1 - 7 - 54
0 - 0 - 0 - 2 - 0

62 off-targets
show all...
527 / fw CCTCCGACTTCCCGGGAGCG TGG
.................... ...
 Cloning / PCR primers
89 93 56 59 87 0.3 0 9 49 38 43 69 71 85 0 - 0 - 0 - 7 - 64
0 - 0 - 0 - 0 - 2

71 off-targets
show all...
592 / rev CGCTCCAGCAGACCGTGGCT CGG
.................... ...
Enzymes: BstDSI, NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BslI, SspDI
 Cloning / PCR primers
89 93 43 43 44 0.2 0 7 47 44 34 60 66 75 0 - 0 - 3 - 3 - 65
0 - 0 - 0 - 0 - 0

71 off-targets
show all...
675 / rev GATTCGGTGTCTGAGCTACT AGG
.................... ...
Enzymes: MaeI
 Cloning / PCR primers
89 95 56 53 71 -0.4 0 49 36 58 41 63 72 87 0 - 0 - 0 - 6 - 41
0 - 0 - 0 - 1 - 1

47 off-targets
show all...
136 / rev CTCTGTGCCGCACCGCCTCT GGG
.................... ...
Inefficient
Enzymes: BstENI, BslI, MnlI
 Cloning / PCR primers
88 90 35 34 36 -0.2 0 2 19 40 24 73 68 79 0 - 0 - 0 - 4 - 113
0 - 0 - 0 - 0 - 0

117 off-targets
show all...
175 / fw AGAGGAGCGCTCGCATCACA AGG
.................... ...
Enzymes: AsuHPI, BstEII, DraIII, MaeIII, Tsp45I
 Cloning / PCR primers
88 90 61 61 39 0.3 82 24 68 51 34 67 64 78 0 - 0 - 0 - 8 - 86
0 - 0 - 0 - 0 - 0

94 off-targets
show all...
507 / rev CCACGCTCCCGGGAAGTCGG AGG
.................... ...
Enzymes: MspI, BshFI, LpnPI, Hpy188I, PspPI
 Cloning / PCR primers
88 93 53 54 79 0.7 0 21 70 49 44 84 63 88 0 - 0 - 0 - 3 - 46
0 - 0 - 0 - 0 - 0

49 off-targets
show all...
608 / fw GGCGCCGAGCCACGGTCTGC TGG
.................... ...
High GC content
Enzymes: BpmI
 Cloning / PCR primers
87 94 34 30 73 -0.3 0 2 58 15 31 65 62 80 0 - 0 - 1 - 5 - 41
0 - 0 - 0 - 0 - 1

47 off-targets
show all...
361 / fw TCCGGGGATGGAGAGCCGAA AGG
.................... ...
Enzymes: NlaIII
 Cloning / PCR primers
86 91 63 64 97 0.2 0 20 50 61 67 75 55 83 0 - 0 - 0 - 3 - 87
0 - 0 - 0 - 0 - 1

90 off-targets
show all...
560 / fw CAGGAGGAGGAGCCGGTCGA TGG
.................... ...
Enzymes: Bsh1285I, BslI, TaqI, MaeI
 Cloning / PCR primers
86 87 50 56 68 0.3 0 2 76 65 69 73 81 87 0 - 0 - 0 - 29 - 298
0 - 0 - 0 - 3 - 0

327 off-targets
show all...
189 / rev CGACCGCGGAGATGGCAGTG GGG
.................... ...
Enzymes: AluBI, BtsIMutI, BtsI, TspRI
 Cloning / PCR primers
85 93 73 76 99 0.0 0 2 64 60 67 72 68 88 0 - 0 - 0 - 4 - 58
0 - 0 - 0 - 2 - 4

62 off-targets
show all...
453 / rev ACCATCTTCTCTGGCGGTTC AGG
.................... ...
Inefficient
Enzymes: MwoI, ApeKI, Fsp4HI, BstV1I
 Cloning / PCR primers
85 94 29 37 1 -0.7 0 2 14 53 47 58 54 60 0 - 0 - 1 - 7 - 88
0 - 0 - 0 - 1 - 1

96 off-targets
show all...
520 / fw AGCCGGCCCTCCGACTTCCC GGG
.................... ...
Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, Cfr9I
 Cloning / PCR primers
85 92 51 51 32 -0.2 0 5 37 45 47 80 75 82 0 - 0 - 1 - 6 - 85
0 - 0 - 1 - 1 - 0

92 off-targets
show all...
309 / fw CCTGGGATTAGGAGCTTACT TGG
.................... ...
 Cloning / PCR primers
84 88 28 40 17 -0.7 0 1 28 40 50 70 64 85 0 - 0 - 0 - 11 - 131
0 - 0 - 0 - 0 - 1

142 off-targets
show all...
311 / fw TGGGATTAGGAGCTTACTTG GGG
.................... ...
Inefficient
 Cloning / PCR primers
84 94 53 47 57 0.1 0 7 64 44 50 75 64 74 0 - 0 - 0 - 12 - 83
0 - 0 - 0 - 3 - 0

95 off-targets
show all...
312 / fw GGGATTAGGAGCTTACTTGG GGG
.................... ...
 Cloning / PCR primers
84 88 61 61 89 0.4 62 29 80 67 65 64 67 84 0 - 0 - 0 - 9 - 94
0 - 0 - 0 - 0 - 2

103 off-targets
show all...
628 / fw TGGAGCGGCCATGCTTGGCC CGG
.................... ...
Inefficient
Enzymes: BshFI, BmeT110I, NlaIV, BslFI, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
 Cloning / PCR primers
84 88 36 36 8 0.2 0 4 50 62 64 65 64 82 0 - 0 - 0 - 11 - 92
0 - 0 - 0 - 1 - 5

103 off-targets
show all...
237 / fw GACACGGCGCTGGGGCTACC CGG
.................... ...
Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I
 Cloning / PCR primers
83 92 48 46 60 -0.6 0 5 36 47 53 58 69 88 0 - 0 - 1 - 2 - 82
0 - 0 - 0 - 1 - 3

85 off-targets
show all...
414 / fw TTGGCACTCTGGTTGGTAAG AGG
.................... ...
Enzymes: BspTNI, BcoDI
 Cloning / PCR primers
83 90 54 48 68 0.0 0 16 33 56 45 71 80 85 0 - 0 - 0 - 5 - 102
0 - 0 - 0 - 0 - 3

107 off-targets
show all...
502 / rev CTCCCGGGAAGTCGGAGGGC CGG
.................... ...
Enzymes: BshFI, MspI, PspPI, LpnPI, NaeI, Cfr10I, BstC8I
 Cloning / PCR primers
83 88 50 50 51 0.5 0 6 54 51 52 58 69 76 0 - 0 - 0 - 15 - 91
0 - 0 - 0 - 0 - 1

106 off-targets
show all...
600 / fw CAGGGCTGGGCGCCGAGCCA CGG
.................... ...
High GC content
Enzymes: BstDSI, BslI, HpyCH4III, BseDI
 Cloning / PCR primers
82 87 42 40 51 0.6 0 4 73 70 59 81 66 76 0 - 0 - 0 - 16 - 170
0 - 0 - 0 - 0 - 0

186 off-targets
show all...
662 / fw CCCTCCGCGGACAGCCTCTC TGG
.................... ...
Inefficient
Enzymes: BshFI, BalI, MwoI, MnlI, BglI, AcoI
 Cloning / PCR primers
82 90 35 34 35 -0.7 0 0 18 43 35 56 70 78 0 - 0 - 0 - 8 - 72
0 - 0 - 0 - 1 - 0

80 off-targets
show all...
747 / fw GCACCCCAGACTGCGCCACC GGG
.................... ...
Enzymes: MspI, NciI, LpnPI, StyD4I
 Cloning / PCR primers
82 91 58 56 89 0.0 0 24 57 48 44 67 60 77 0 - 0 - 0 - 10 - 108
0 - 0 - 0 - 0 - 1

118 off-targets
show all...
96 / fw GTAATGACGATTGCAGCAGG AGG
.................... ...
Enzymes: EcoP15I, BtsCI
 Cloning / PCR primers
81 93 65 68 79 0.9 78 78 88 75 35 66 70 78 0 - 0 - 0 - 12 - 56
0 - 0 - 0 - 2 - 5

68 off-targets
show all...
310 / fw CTGGGATTAGGAGCTTACTT GGG
.................... ...
Inefficient
 Cloning / PCR primers
81 89 38 38 14 -0.2 0 15 33 43 52 75 65 82 0 - 0 - 0 - 9 - 109
0 - 0 - 0 - 1 - 3

118 off-targets
show all...
488 / rev GAGGGCCGGCTCGCGGGCTC AGG
.................... ...
High GC content
Enzymes: Bsp1286I, BstDEI, BstC8I, Eco24I, BspCNI
 Cloning / PCR primers
81 93 36 37 54 0.1 0 0 58 80 54 77 64 73 0 - 0 - 1 - 8 - 76
0 - 0 - 0 - 2 - 0

85 off-targets
show all...
665 / rev CTGAGCTACTAGGTTGCCCT TGG
.................... ...
Enzymes: BshFI, BalI, BseDI, ErhI, LpnPI, AcoI
 Cloning / PCR primers
81 88 51 49 11 0.1 0 7 54 50 48 70 65 78 0 - 0 - 2 - 13 - 122
0 - 0 - 0 - 1 - 0

137 off-targets
show all...
157 / fw CCAGAGGCGGTGCGGCACAG AGG
.................... ...
Enzymes: HinP1I, BseRI
 Cloning / PCR primers
80 88 59 61 94 0.5 79 33 80 72 55 79 70 88 0 - 0 - 0 - 10 - 133
0 - 0 - 0 - 0 - 0

143 off-targets
show all...
235 / rev AAGGCGACAAGGCAGGCGCC GGG
.................... ...
Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I
 Cloning / PCR primers
80 90 52 51 65 0.2 0 11 65 70 61 68 68 70 0 - 0 - 1 - 12 - 93
0 - 0 - 0 - 0 - 0

106 off-targets
show all...
242 / rev AGGAGCTAAGGCGACAAGGC AGG
.................... ...
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI, BstC8I
 Cloning / PCR primers
80 88 49 42 73 0.4 0 5 60 42 37 63 64 72 0 - 0 - 1 - 13 - 149
0 - 0 - 0 - 0 - 0

163 off-targets
show all...
246 / rev GAAGAGGAGCTAAGGCGACA AGG
.................... ...
Enzymes: BstC8I
 Cloning / PCR primers
80 82 61 62 95 0.3 62 15 83 37 44 63 65 81 0 - 0 - 1 - 17 - 201
0 - 0 - 0 - 2 - 1

219 off-targets
show all...
613 / fw CGAGCCACGGTCTGCTGGAG CGG
.................... ...
Enzymes: BshFI, TauI, MwoI, BpmI, NlaIII, AcoI, Fsp4HI
 Cloning / PCR primers
79 91 49 52 47 0.2 0 5 56 62 52 67 68 77 0 - 0 - 1 - 10 - 58
0 - 0 - 0 - 3 - 10

69 off-targets
show all...
636 / rev AGGCTGTCCGCGGAGGGGAC TGG
.................... ...
Enzymes: BmuI, Bse1I, Eam1105I, BslFI, BslI
 Cloning / PCR primers
79 88 30 23 7 -0.3 0 0 47 59 46 77 69 76 0 - 0 - 2 - 6 - 99
0 - 0 - 0 - 0 - 1

107 off-targets
show all...
649 / fw GGGACCCCCAGTCCCCTCCG CGG
.................... ...
High GC content
Enzymes: BstDSI, SacII, MnlI, BspFNI, MspA1I, BseDI, BslI, SsiI
 Cloning / PCR primers
79 88 68 74 92 0.4 0 47 85 70 63 70 68 81 0 - 0 - 1 - 9 - 104
0 - 0 - 0 - 1 - 1

114 off-targets
show all...
415 / fw TGGCACTCTGGTTGGTAAGA GGG
.................... ...
Enzymes: BspTNI, BcoDI
 Cloning / PCR primers
78 84 45 50 13 -0.0 0 27 35 56 47 53 77 79 0 - 0 - 1 - 13 - 125
0 - 0 - 0 - 2 - 3

139 off-targets
show all...
518 / rev GGCCTCCCCCGCCACGCTCC CGG
.................... ...
High GC content
Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, LmnI, Cfr9I
 Cloning / PCR primers
78 88 32 27 27 -0.4 0 1 38 37 30 77 71 77 0 - 0 - 3 - 13 - 134
0 - 0 - 0 - 2 - 2

150 off-targets
show all...
616 / rev TGGGGGTCCCGGGCCAAGCA TGG
.................... ...
Enzymes: BshFI, TauI, NlaIII, AcoI, SsiI, Fsp4HI
 Cloning / PCR primers
78 87 45 46 28 0.3 0 4 62 53 58 78 72 89 0 - 0 - 0 - 13 - 128
0 - 0 - 0 - 1 - 0

141 off-targets
show all...
191 / rev GGCGACCGCGGAGATGGCAG TGG
.................... ...
Enzymes: MwoI, BtsIMutI, BtsI, TspRI
 Cloning / PCR primers
77 88 46 53 94 0.6 0 10 86 48 56 79 64 69 0 - 0 - 0 - 10 - 105
0 - 0 - 0 - 1 - 1

115 off-targets
show all...
633 / rev CTGTCCGCGGAGGGGACTGG GGG
.................... ...
Enzymes: Bse1I, NlaIV, Eam1105I, EcoO109I, PpuMI, PspPI, Bme18I, BmuI, KflI
 Cloning / PCR primers
77 86 44 43 77 0.5 0 20 65 67 70 67 65 78 0 - 0 - 0 - 12 - 107
0 - 0 - 0 - 6 - 5

119 off-targets
show all...
646 / rev TTGGCCAGAGAGGCTGTCCG CGG
.................... ...
Enzymes: BstDSI, SacII, BspFNI, MspA1I, BseDI, BslI, SsiI
 Cloning / PCR primers
77 86 63 62 83 0.4 54 63 64 70 66 78 68 87 0 - 0 - 0 - 17 - 130
0 - 0 - 0 - 0 - 2

147 off-targets
show all...
149 / fw GCTTCCTCCCAGAGGCGGTG CGG
.................... ...
Enzymes: TauI, Fsp4HI, BslI
 Cloning / PCR primers
76 80 61 63 84 0.1 0 34 77 61 63 64 71 85 0 - 0 - 3 - 24 - 165
0 - 0 - 0 - 2 - 1

192 off-targets
show all...
436 / rev TTCAGGCAGCGGGCTGCTCA CGG
.................... ...
Enzymes: BceAI
 Cloning / PCR primers
76 85 52 52 41 0.1 0 4 35 51 55 77 82 88 0 - 0 - 0 - 16 - 107
0 - 0 - 0 - 0 - 2

123 off-targets
show all...
462 / rev CCGTGGGGCACCATCTTCTC TGG
.................... ...
Inefficient
 Cloning / PCR primers
76 93 39 41 30 -0.2 0 2 36 57 44 72 66 67 0 - 0 - 2 - 22 - 81
0 - 0 - 0 - 2 - 2

105 off-targets
show all...
623 / fw TCTGCTGGAGCGGCCATGCT TGG
.................... ...
Enzymes: BshFI, MspI, PspPI, NciI, NlaIII, LpnPI, StyD4I
 Cloning / PCR primers
76 89 43 37 60 -0.2 0 4 27 50 51 72 68 90 0 - 0 - 1 - 11 - 112
0 - 0 - 0 - 0 - 3

124 off-targets
show all...
634 / rev GCTGTCCGCGGAGGGGACTG GGG
.................... ...
Enzymes: Bse1I, NlaIV, Eam1105I, BslFI, EcoO109I, PpuMI, PspPI, Bme18I, BmuI, KflI
 Cloning / PCR primers
75 86 39 34 99 0.2 0 7 72 64 63 83 66 81 0 - 0 - 1 - 9 - 105
0 - 0 - 0 - 1 - 1

115 off-targets
show all...
730 / rev GTTCCCGGTGGCGCAGTCTG GGG
.................... ...
Enzymes: BsgI, HpyCH4V
 Cloning / PCR primers
75 83 58 59 95 0.2 0 19 57 75 55 73 57 73 0 - 0 - 1 - 6 - 130
0 - 0 - 0 - 0 - 0

137 off-targets
show all...
289 / rev CCAAGTAAGCTCCTAATCCC AGG
.................... ...
Enzymes: NlaIV, BseDI, LpnPI, BstNI, LmnI, StyD4I
 Cloning / PCR primers
74 92 54 58 65 0.2 0 29 62 40 37 63 70 79 0 - 0 - 0 - 7 - 109
0 - 0 - 0 - 0 - 0

116 off-targets
show all...
447 / rev TTCTCTGGCGGTTCAGGCAG CGG
.................... ...
Enzymes: MwoI, MspA1I, ApeKI, BstV1I, Fsp4HI, BstC8I
 Cloning / PCR primers
74 81 39 39 49 0.9 0 27 57 54 43 60 67 82 0 - 0 - 1 - 15 - 147
0 - 0 - 0 - 1 - 0

163 off-targets
show all...
635 / rev GGCTGTCCGCGGAGGGGACT GGG
.................... ...
Enzymes: Bse1I, NlaIV, Eam1105I, BslFI, PspPI, Bme18I, BmuI
 Cloning / PCR primers
74 88 25 25 20 -0.4 0 1 40 58 56 61 67 77 0 - 0 - 0 - 14 - 97
0 - 0 - 0 - 0 - 1

111 off-targets
show all...
403 / fw TGGGGGTCAGCTTGGCACTC TGG
.................... ...
 Cloning / PCR primers
73 86 41 43 13 -0.4 0 1 37 59 50 68 69 72 0 - 0 - 3 - 13 - 139
0 - 0 - 0 - 1 - 2

155 off-targets
show all...
718 / fw GGATTTCTATGAAGAAATCG AGG
.................... ...
Enzymes: AsuHPI, TaqI
 Cloning / PCR primers
73 83 68 71 93 0.4 0 60 86 17 19 62 56 74 0 - 0 - 1 - 16 - 121
0 - 0 - 0 - 1 - 2

138 off-targets
show all...
329 / rev CCATCCCCGGACTCTCCAGA GGG
.................... ...
Enzymes: LpnPI, Hpy188III, BslI
 Cloning / PCR primers
72 86 50 51 69 0.3 81 72 68 36 42 62 73 80 0 - 0 - 1 - 21 - 103
0 - 0 - 0 - 3 - 2

125 off-targets
show all...
349 / fw CCCTCTGGAGAGTCCGGGGA TGG
.................... ...
Enzymes: NciI, BtsCI, BseDI, StyD4I
 Cloning / PCR primers
72 83 46 46 39 0.2 0 10 58 64 62 58 63 57 0 - 0 - 4 - 11 - 317
0 - 0 - 1 - 0 - 2

332 off-targets
show all...
328 / rev CATCCCCGGACTCTCCAGAG GGG
.................... ...
Enzymes: LpnPI, Hpy188III, BslI
 Cloning / PCR primers
71 85 62 58 96 0.6 83 3 68 61 36 75 75 83 0 - 0 - 1 - 20 - 108
0 - 0 - 1 - 2 - 6

129 off-targets
show all...
407 / fw GGTCAGCTTGGCACTCTGGT TGG
.................... ...
 Cloning / PCR primers
71 88 46 49 62 -0.4 0 25 66 35 46 68 70 69 0 - 0 - 2 - 22 - 103
0 - 0 - 0 - 0 - 4

127 off-targets
show all...
345 / fw CCCCCCCTCTGGAGAGTCCG GGG
.................... ...
Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
 Cloning / PCR primers
70 83 56 54 82 0.1 0 57 44 59 59 63 64 74 0 - 0 - 3 - 10 - 128
0 - 0 - 2 - 1 - 0

141 off-targets
show all...
629 / fw GGAGCGGCCATGCTTGGCCC GGG
.................... ...
Enzymes: BshFI, BmeT110I, NlaIV, BslFI, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
 Cloning / PCR primers
70 87 43 43 31 0.5 0 24 91 52 53 79 64 70 0 - 0 - 2 - 10 - 123
0 - 0 - 0 - 4 - 4

135 off-targets
show all...
343 / fw CTCCCCCCCTCTGGAGAGTC CGG
.................... ...
Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
 Cloning / PCR primers
69 82 32 32 7 -0.7 0 1 11 59 46 49 59 70 0 - 0 - 4 - 24 - 152
0 - 0 - 0 - 2 - 4

180 off-targets
show all...
384 / fw ACATGGTTATGTTTCTGGAT GGG
.................... ...
Enzymes: Hpy188III, BtsCI
 Cloning / PCR primers
69 80 42 52 3 0.1 0 12 36 25 46 66 65 77 0 - 0 - 4 - 18 - 179
0 - 0 - 2 - 2 - 3

201 off-targets
show all...
669 / fw CGGACAGCCTCTCTGGCCAA GGG
.................... ...
Enzymes: BshFI, ErhI, BalI, BseDI, AcoI
 Cloning / PCR primers
69 86 58 57 42 0.6 0 26 57 83 62 61 63 81 0 - 0 - 2 - 15 - 102
0 - 0 - 1 - 1 - 5

119 off-targets
show all...
141 / fw GCAATGGGGCTTCCTCCCAG AGG
.................... ...
Enzymes: BstENI, LpnPI, BslI
 Cloning / PCR primers
68 83 68 65 97 0.7 0 39 88 69 60 82 69 86 0 - 0 - 2 - 18 - 150
0 - 0 - 1 - 6 - 7

170 off-targets
show all...
539 / rev CATCGACCGGCTCCTCCTCC TGG
.................... ...
Enzymes: BshFI, MnlI, LpnPI, BstNI, BslI, StyD4I
 Cloning / PCR primers
68 88 38 39 11 -0.2 0 0 34 18 16 80 73 66 0 - 0 - 0 - 16 - 97
0 - 0 - 0 - 4 - 12

113 off-targets
show all...
582 / fw GACTAGCAGGCAGTGCTGCA GGG
.................... ...
Enzymes: PstI, BfmI, ApeKI, Fsp4HI, HpyCH4V
 Cloning / PCR primers
68 88 54 53 98 0.0 63 37 58 45 51 62 64 89 0 - 0 - 3 - 21 - 126
0 - 0 - 0 - 4 - 4

150 off-targets
show all...
206 / fw GCTCCCCACTGCCATCTCCG CGG
.................... ...
Enzymes: BstDSI, MwoI, SacII, BspFNI, MspA1I, BseDI, Bsh1285I, SsiI
 Cloning / PCR primers
67 81 70 72 99 0.2 87 92 79 37 43 80 51 64 0 - 0 - 1 - 17 - 193
0 - 0 - 0 - 0 - 2

211 off-targets
show all...
326 / rev TCCCCGGACTCTCCAGAGGG GGG
.................... ...
Enzymes: BslI
 Cloning / PCR primers
67 72 51 50 86 0.9 0 13 71 67 54 64 72 79 0 - 0 - 1 - 26 - 161
0 - 0 - 0 - 3 - 8

188 off-targets
show all...
379 / fw AAAGGACATGGTTATGTTTC TGG
.................... ...
Inefficient
Enzymes: Hpy188III, BtsCI
 Cloning / PCR primers
67 79 21 16 4 -0.9 0 1 18 36 31 76 55 67 0 - 0 - 3 - 32 - 234
0 - 0 - 0 - 2 - 2

269 off-targets
show all...
656 / rev TAGGTTGCCCTTGGCCAGAG AGG
.................... ...
Enzymes: LpnPI, MwoI, BglI
 Cloning / PCR primers
67 79 58 60 67 0.2 56 10 56 88 49 68 72 84 0 - 0 - 2 - 17 - 130
0 - 0 - 1 - 2 - 14

149 off-targets
show all...
144 / fw ATGGGGCTTCCTCCCAGAGG CGG
.................... ...
Enzymes: BstENI, BslI
 Cloning / PCR primers
66 79 63 60 61 1.1 0 11 86 83 81 79 64 81 0 - 0 - 7 - 24 - 132
0 - 0 - 3 - 6 - 8

163 off-targets
show all...
330 / rev TCCATCCCCGGACTCTCCAG AGG
.................... ...
Enzymes: LpnPI, Hpy188III, BslI
 Cloning / PCR primers
66 82 51 53 94 0.6 0 14 71 60 52 80 73 74 0 - 0 - 3 - 23 - 132
0 - 0 - 0 - 1 - 2

158 off-targets
show all...
321 / rev GGACTCTCCAGAGGGGGGGA GGG
.................... ...
Enzymes: BslI
 Cloning / PCR primers
65 66 46 45 70 0.3 0 13 82 54 59 69 65 74 0 - 0 - 4 - 22 - 231
0 - 0 - 0 - 0 - 3

257 off-targets
show all...
344 / fw TCCCCCCCTCTGGAGAGTCC GGG
.................... ...
Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
 Cloning / PCR primers
65 79 43 42 16 -0.3 0 2 31 40 33 72 59 65 0 - 0 - 1 - 22 - 154
0 - 0 - 0 - 6 - 8

177 off-targets
show all...
383 / fw GACATGGTTATGTTTCTGGA TGG
.................... ...
Enzymes: Hpy188III, BtsCI
 Cloning / PCR primers
65 74 47 56 47 -0.3 0 8 71 33 32 65 67 80 0 - 0 - 1 - 34 - 174
0 - 0 - 0 - 3 - 4

209 off-targets
show all...
446 / rev TCTCTGGCGGTTCAGGCAGC GGG
.................... ...
Enzymes: MspA1I, BstC8I, ApeKI, Fsp4HI, BstV1I
 Cloning / PCR primers
65 85 41 44 36 0.3 0 13 36 63 34 76 73 68 0 - 0 - 0 - 15 - 169
0 - 0 - 0 - 1 - 1

184 off-targets
show all...
367 / fw GATGGAGAGCCGAAAGGACA TGG
.................... ...
Enzymes: NlaIII, SmiMI
 Cloning / PCR primers
64 80 56 59 57 0.3 52 30 83 50 56 69 52 74 0 - 0 - 0 - 23 - 219
0 - 0 - 0 - 2 - 0

242 off-targets
show all...
517 / rev GCCTCCCCCGCCACGCTCCC GGG
.................... ...
High GC content
Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, LmnI, Cfr9I
 Cloning / PCR primers
64 81 39 36 79 -0.5 0 10 44 46 36 64 75 85 0 - 0 - 4 - 24 - 244
0 - 0 - 0 - 0 - 3

272 off-targets
show all...
283 / fw CTCTTCTCAGCCAAGATCCC AGG
.................... ...
Enzymes: NlaIV, NdeII, MflI, BseDI, LpnPI, BstNI, StyD4I, PasI
 Cloning / PCR primers
63 76 43 45 32 -0.3 0 7 28 31 50 67 66 81 0 - 0 - 3 - 17 - 285
0 - 0 - 0 - 1 - 2

305 off-targets
show all...
322 / rev CGGACTCTCCAGAGGGGGGG AGG
.................... ...
Enzymes: BslI
 Cloning / PCR primers
63 73 30 33 85 0.5 0 4 77 78 60 58 67 73 0 - 0 - 1 - 10 - 175
0 - 0 - 0 - 0 - 5

186 off-targets
show all...
342 / rev TCCTTTCGGCTCTCCATCCC CGG
.................... ...
Enzymes: MspI, BseDI, NciI, LpnPI, StyD4I, HinfI, BccI
 Cloning / PCR primers
63 82 45 44 25 -0.4 0 3 26 40 52 67 64 66 0 - 0 - 0 - 27 - 182
0 - 0 - 0 - 4 - 6

209 off-targets
show all...
386 / fw ATGGTTATGTTTCTGGATGG GGG
.................... ...
Enzymes: BtsCI
 Cloning / PCR primers
63 80 57 54 47 0.4 61 5 64 78 68 76 51 80 0 - 0 - 1 - 37 - 202
0 - 0 - 0 - 9 - 4

240 off-targets
show all...
395 / fw TTTCTGGATGGGGGTCAGCT TGG
.................... ...
Enzymes: AluBI
 Cloning / PCR primers
63 81 43 47 43 -0.3 0 13 37 43 38 72 64 80 0 - 0 - 9 - 50 - 159
0 - 0 - 2 - 5 - 5

218 off-targets
show all...
325 / rev CCCCGGACTCTCCAGAGGGG GGG
.................... ...
Enzymes: BslI
 Cloning / PCR primers
62 81 57 50 81 0.7 0 12 78 61 66 79 70 79 1 - 0 - 3 - 16 - 107
1 - 0 - 1 - 2 - 4

127 off-targets
show all...
281 / rev GCTCCTAATCCCAGGCTCCC TGG
.................... ...
Enzymes: NlaIV, NdeII, BspPI, BseDI, MflI, LpnPI, BstNI, LmnI, StyD4I, PasI
 Cloning / PCR primers
61 72 42 42 89 -0.6 0 5 45 9 24 62 66 85 0 - 0 - 5 - 23 - 246
0 - 0 - 1 - 6 - 13

274 off-targets
show all...
298 / fw ATCCCAGGGAGCCTGGGATT AGG
.................... ...
Enzymes: AluBI
 Cloning / PCR primers
61 78 39 43 38 -0.4 0 0 24 54 47 64 62 71 0 - 0 - 2 - 34 - 222
0 - 0 - 0 - 4 - 8

258 off-targets
show all...
668 / fw GCGGACAGCCTCTCTGGCCA AGG
.................... ...
Enzymes: BshFI, BalI, MwoI, BseDI, BglI, ErhI, AcoI
 Cloning / PCR primers
61 83 44 43 50 0.3 0 9 79 39 45 66 65 74 0 - 0 - 4 - 22 - 265
0 - 0 - 0 - 5 - 3

291 off-targets
show all...
262 / rev CTGGGATCTTGGCTGAGAAG AGG
.................... ...
Enzymes: AluBI, BseRI, MboII
 Cloning / PCR primers
60 75 52 58 39 0.6 55 28 69 64 38 78 70 73 0 - 0 - 7 - 33 - 251
0 - 0 - 2 - 4 - 2

291 off-targets
show all...
356 / rev GAAACATAACCATGTCCTTT CGG
.................... ...
Inefficient
 Cloning / PCR primers
60 82 39 42 53 -0.8 0 9 44 20 26 58 52 74 0 - 0 - 6 - 35 - 221
0 - 0 - 1 - 3 - 7

262 off-targets
show all...
472 / fw GCCTGAACCGCCAGAGAAGA TGG
.................... ...
Enzymes: Bsp1286I, BanI, BaeGI, NlaIV, MboII
 Cloning / PCR primers
60 82 46 47 52 0.0 0 16 60 45 41 48 65 63 0 - 0 - 1 - 30 - 148
0 - 0 - 0 - 3 - 0

179 off-targets
show all...
327 / rev ATCCCCGGACTCTCCAGAGG GGG
.................... ...
Enzymes: Hpy188III, BslI
 Cloning / PCR primers
59 63 52 49 81 0.6 50 8 63 60 59 70 74 83 0 - 0 - 4 - 16 - 176
0 - 0 - 4 - 6 - 3

196 off-targets
show all...
3 / rev TCTGCCACAGTGAAGATGAA AGG
.................... ...
Enzymes: TspDTI
 Cloning / PCR primers
57 75 57 62 79 -0.3 77 48 38 32 32 69 72 82 0 - 1 - 5 - 40 - 282
0 - 0 - 1 - 4 - 8

328 off-targets
show all...
126 / fw CTTAGAAAGAAGAGGGCAAT GGG
.................... ...
Enzymes: BseMI
 Cloning / PCR primers
57 77 43 42 41 -0.3 0 2 24 52 40 60 66 76 0 - 0 - 6 - 38 - 270
0 - 0 - 0 - 7 - 2

314 off-targets
show all...
280 / rev CTCCTAATCCCAGGCTCCCT GGG
.................... ...
Enzymes: NlaIV, NdeII, BspPI, BseDI, MflI, LpnPI, BstNI, LmnI, StyD4I, PasI
 Cloning / PCR primers
57 77 49 43 31 -0.3 0 7 31 40 48 68 66 73 0 - 0 - 6 - 32 - 198
0 - 0 - 1 - 9 - 6

236 off-targets
show all...
581 / fw GGACTAGCAGGCAGTGCTGC AGG
.................... ...
Enzymes: HpyCH4V, PstI, ApeKI, TspRI, BfmI, Fsp4HI
 Cloning / PCR primers
56 80 38 40 36 -0.1 0 2 59 15 32 70 64 48 0 - 0 - 4 - 32 - 196
0 - 0 - 0 - 2 - 14

232 off-targets
show all...
292 / fw GCCAAGATCCCAGGGAGCCT GGG
.................... ...
Inefficient
Enzymes: StyD4I, LpnPI, NlaIV, BstNI, BseDI
 Cloning / PCR primers
55 75 52 50 78 0.1 0 41 74 31 52 58 70 81 0 - 0 - 4 - 30 - 244
0 - 0 - 1 - 7 - 9

278 off-targets
show all...
482 / fw CCAGAGAAGATGGTGCCCCA CGG
.................... ...
Enzymes: Bsp1286I, BstDSI, BaeGI, HpyCH4III, BseDI
 Cloning / PCR primers
55 79 69 63 89 0.5 87 84 82 64 57 75 67 90 0 - 1 - 17 - 30 - 184
0 - 0 - 2 - 3 - 1

232 off-targets
show all...
273 / rev TCCCAGGCTCCCTGGGATCT TGG
.................... ...
Enzymes: BspPI, BstDEI, MflI, NdeII
 Cloning / PCR primers
54 72 34 33 5 -0.5 0 0 16 43 22 63 64 81 0 - 0 - 4 - 46 - 325
0 - 0 - 1 - 4 - 16

375 off-targets
show all...
626 / rev CGGAGGGGACTGGGGGTCCC GGG
.................... ...
High GC content
Enzymes: BshFI, BmeT110I, NlaIV, Eam1105I, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
 Cloning / PCR primers
54 69 39 41 48 -0.2 0 2 34 80 76 68 64 76 0 - 0 - 0 - 24 - 268
0 - 0 - 0 - 4 - 10

292 off-targets
show all...
284 / fw TCTTCTCAGCCAAGATCCCA GGG
.................... ...
Enzymes: NlaIV, MflI, BseDI, LpnPI, BstNI, StyD4I, PasI
 Cloning / PCR primers
52 76 63 56 92 0.3 66 70 63 35 44 52 66 84 0 - 1 - 6 - 38 - 286
0 - 0 - 1 - 1 - 1

331 off-targets
show all...
320 / rev GACTCTCCAGAGGGGGGGAG GGG
.................... ...
 Cloning / PCR primers
52 63 37 30 87 0.2 0 9 70 65 59 60 68 82 0 - 0 - 5 - 29 - 313
0 - 0 - 0 - 3 - 8

347 off-targets
show all...
587 / fw GCAGGCAGTGCTGCAGGGCT GGG
.................... ...
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI
 Cloning / PCR primers
52 70 30 33 33 0.4 0 9 67 72 63 66 60 85 0 - 0 - 1 - 51 - 354
0 - 0 - 1 - 3 - 12

406 off-targets
show all...
127 / fw TTAGAAAGAAGAGGGCAATG GGG
.................... ...
Enzymes: BseMI
 Cloning / PCR primers
51 53 73 74 99 0.4 0 41 60 42 45 66 68 92 0 - 0 - 4 - 64 - 490
0 - 0 - 0 - 4 - 7

558 off-targets
More than 500 off-targets, showing 7 with score >1.0 show all...
291 / fw AGCCAAGATCCCAGGGAGCC TGG
.................... ...
Inefficient
Enzymes: StyD4I, LpnPI, NlaIV, BstNI, BseDI
 Cloning / PCR primers
51 70 41 39 37 -0.6 0 0 25 16 50 53 66 81 0 - 0 - 2 - 43 - 315
0 - 0 - 0 - 7 - 10

360 off-targets
show all...
118 / fw GATGACAGCTTAGAAAGAAG AGG
.................... ...
Enzymes: MboII
 Cloning / PCR primers
50 68 61 61 96 0.8 0 27 89 30 25 66 72 71 0 - 0 - 6 - 33 - 297
0 - 0 - 1 - 5 - 13

336 off-targets
show all...
254 / rev TTGGCTGAGAAGAGGAGCTA AGG
.................... ...
Enzymes: AluBI, BstDEI
 Cloning / PCR primers
50 71 55 59 68 0.5 0 7 70 56 33 61 71 84 0 - 0 - 5 - 49 - 301
0 - 0 - 1 - 9 - 5

355 off-targets
show all...
544 / fw GCGTGGCGGGGGAGGCCAGG AGG
.................... ...
High GC content
Enzymes: StyD4I, BseRI, LpnPI, BstNI, BslI
 Cloning / PCR primers
47 65 43 47 99 0.6 0 7 80 83 74 72 62 88 0 - 0 - 6 - 46 - 427
0 - 0 - 3 - 9 - 8

479 off-targets
show all...
181 / rev GAGATGGCAGTGGGGAGCTA GGG
.................... ...
Enzymes: AluBI, Tsp45I, BstEII, MaeIII, MaeI
 Cloning / PCR primers
46 66 54 49 62 -0.0 0 37 79 68 59 67 66 76 0 - 0 - 11 - 44 - 439
0 - 0 - 3 - 4 - 7

494 off-targets
show all...
385 / fw CATGGTTATGTTTCTGGATG GGG
.................... ...
Enzymes: Hpy188III, BtsCI
 Cloning / PCR primers
46 71 37 44 16 0.1 0 2 40 54 44 75 58 76 0 - 0 - 3 - 30 - 369
0 - 0 - 0 - 1 - 5

402 off-targets
show all...
541 / fw GGAGCGTGGCGGGGGAGGCC AGG
.................... ...
Inefficient
High GC content
Enzymes: BshFI, MwoI, BseRI, LpnPI, BstNI, StyD4I
 Cloning / PCR primers
46 73 37 38 73 0.0 0 1 74 59 48 68 73 80 0 - 0 - 2 - 44 - 468
0 - 0 - 0 - 5 - 9

514 off-targets
More than 500 off-targets, showing 13 with score >1.0 show all...
125 / fw GCTTAGAAAGAAGAGGGCAA TGG
.................... ...
Enzymes: BseMI
 Cloning / PCR primers
45 61 55 59 93 0.3 0 47 83 32 29 67 66 78 0 - 0 - 5 - 50 - 442
0 - 0 - 0 - 6 - 18

497 off-targets
show all...
586 / fw AGCAGGCAGTGCTGCAGGGC TGG
.................... ...
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI
 Cloning / PCR primers
45 68 35 29 38 0.0 0 0 61 37 42 59 62 82 0 - 1 - 9 - 52 - 431
0 - 0 - 1 - 4 - 6

493 off-targets
show all...
319 / rev ACTCTCCAGAGGGGGGGAGG GGG
.................... ...
 Cloning / PCR primers
38 49 42 34 93 0.6 0 7 66 75 56 77 67 82 0 - 0 - 6 - 60 - 542
0 - 0 - 0 - 6 - 7

608 off-targets
More than 500 off-targets, showing 17 with score >1.0 show all...
119 / fw ATGACAGCTTAGAAAGAAGA GGG
.................... ...
Enzymes: BseMI, MboII
 Cloning / PCR primers
36 63 59 62 37 0.6 0 55 73 59 25 49 70 58 0 - 2 - 7 - 81 - 397
0 - 0 - 1 - 10 - 17

487 off-targets
show all...
334 / fw GTTTTCCCCCTCCCCCCCTC TGG
.................... ...
Not with U6/U3
Enzymes: BpmI, BslI, Hpy188III, MnlI
 Cloning / PCR primers
36 59 36 36 47 -0.5 0 7 47 44 38 74 68 72 0 - 1 - 7 - 67 - 471
0 - 0 - 2 - 3 - 12

546 off-targets
More than 500 off-targets, showing 23 with score >1.0 show all...
627 / rev GCGGAGGGGACTGGGGGTCC CGG
.................... ...
High GC content
Enzymes: BshFI, BmeT110I, NlaIV, Eam1105I, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
 Cloning / PCR primers
34 77 34 37 25 0.0 0 0 57 85 65 63 63 71 0 - 1 - 2 - 25 - 332
0 - 1 - 1 - 4 - 4

360 off-targets
show all...
536 / fw TCCCGGGAGCGTGGCGGGGG AGG
.................... ...
High GC content
Enzymes: BshFI, StyD4I, LpnPI, MwoI, BstNI
 Cloning / PCR primers
33 70 41 34 81 0.8 0 1 63 79 66 73 64 71 0 - 1 - 2 - 38 - 439
0 - 0 - 0 - 2 - 5

480 off-targets
show all...
182 / rev GGAGATGGCAGTGGGGAGCT AGG
.................... ...
Enzymes: AluBI, Tsp45I, MaeIII, MaeI
 Cloning / PCR primers
26 51 39 39 64 -0.1 0 2 56 57 66 58 66 79 0 - 3 - 12 - 78 - 964
0 - 0 - 3 - 7 - 13

1057 off-targets
More than 500 off-targets, showing 23 with score >1.0 show all...
459 / rev TGGGGCACCATCTTCTCTGG CGG
.................... ...
 Cloning / PCR primers
24 72 66 60 37 0.6 0 36 71 69 62 80 60 75 0 - 0 - 1241 - 21 - 138
0 - 0 - 1 - 3 - 1

1400 off-targets
More than 500 off-targets, showing 24 with score >0.5 show all...
547 / fw TGGCGGGGGAGGCCAGGAGG AGG
.................... ...
High GC content
Enzymes: BseRI, NlaIV, BslI
 Cloning / PCR primers
19 33 37 34 80 1.3 0 3 82 95 77 77 57 74 0 - 0 - 6 - 158 - 1190
0 - 0 - 1 - 18 - 30

1354 off-targets
More than 500 off-targets, showing 12 with score >2.0 show all...
641 / rev CAGAGAGGCTGTCCGCGGAG GGG
.................... ...
Enzymes: BstDSI, Bse1I, BslFI, SacII, MspA1I, BseDI, BslI
 Cloning / PCR primers
16 32 59 64 82 0.6 64 12 67 83 78 70 70 72 0 - 0 - 0 - 1338 - 568
0 - 0 - 0 - 0 - 1

1906 off-targets
More than 500 off-targets, showing 4 with score >0.5 show all...
553 / fw GGGAGGCCAGGAGGAGGAGC CGG
.................... ...
Enzymes: NlaIV, MspI, TaqI, BseRI, LpnPI, Bsh1285I, Cfr10I
 Cloning / PCR primers
5 15 48 48 55 0.5 0 18 78 71 54 55 66 56 0 - 2 - 35 - 378 - 4403
0 - 0 - 9 - 72 - 1098

4818 off-targets
More than 500 off-targets, showing 20 with score >2.0 show all...
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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.