CRISPOR

DNA break repair outcome predictions

Guide: GGTCAGCTTGGCACTCTGGT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAGCAGC`	(Wild-type sequence)
491	`TCTGGATGGGGGTCAGCTTGGCACTCTGGT----AAGAGGGTCTCTAATTTGTCCGAAGC`	`4 bp deleted → frameshift`
274	`TCTGGATGGGGGTCAGCTTGGCACTCTGGT------------CTCTAATTTGTCCGAAGC`	`12 bp deleted → no frameshift`
274	`TCTGGATGGGGGTCAGCTTGG------------TAAGAGGGTCTCTAATTTGTCCGAAGC`	`12 bp deleted → no frameshift`
232	`TCTGGATGGGGGTCAGCTTGGCACTCT-------------------AATTTGTCCGAAGC`	`19 bp deleted → frameshift`
230	`TCTGGATGGGGGTCAGCTTGGCACTCTGG-----------GTCTCTAATTTGTCCGAAGC`	`11 bp deleted → frameshift`
211	`TCTGGATGGGGGTC-----------------------------TCTAATTTGTCCGAAGC`	`29 bp deleted → frameshift`
175	`TCTGGATGGGGGT---------------------AAGAGGGTCTCTAATTTGTCCGAAGC`	`21 bp deleted → no frameshift`
170	`TCTGGATGGGGGTCAGCTTGGCACTCT-----------------CTAATTTGTCCGAAGC`	`17 bp deleted → frameshift`
154	`TCTGGATGGGGGTCAGCTTGG-------------------GTCTCTAATTTGTCCGAAGC`	`19 bp deleted → frameshift`
150	`TCTGGATGG------------------------TAAGAGGGTCTCTAATTTGTCCGAAGC`	`24 bp deleted → no frameshift`
147	`TCTGGATGGGGGTCAGCTTGG--------------------TCTCTAATTTGTCCGAAGC`	`20 bp deleted → frameshift`
133	`TCTGGATGGGG------------------------------TCTCTAATTTGTCCGAAGC`	`30 bp deleted → no frameshift`
133	`TCTGGATGGGG----------------------TAAGAGGGTCTCTAATTTGTCCGAAGC`	`22 bp deleted → frameshift`
127	`TCTGGATGGG-------------------------------TCTCTAATTTGTCCGAAGC`	`31 bp deleted → frameshift`
126	`TCTGGATGGG-----------------------TAAGAGGGTCTCTAATTTGTCCGAAGC`	`23 bp deleted → frameshift`
123	`TCTGG----------------------------TAAGAGGGTCTCTAATTTGTCCGAAGC`	`28 bp deleted → frameshift`
105	`TCTGGATGGGGGTCAGCTTGGCACT---------------------AATTTGTCCGAAGC`	`21 bp deleted → no frameshift`
105	`TCTGGATGGGGGTCAG---------------------AGGGTCTCTAATTTGTCCGAAGC`	`21 bp deleted → no frameshift`
98	`TCTGGATGGGGG----------------------------GTCTCTAATTTGTCCGAAGC`	`28 bp deleted → frameshift`
95	`TCTGGATGGGGGTCAGCTTGGCACTCTGGT-----------------------CCGAAGC`	`23 bp deleted → frameshift`
95	`TCTGGATGGGGGTCAG-----------------------GGTCTCTAATTTGTCCGAAGC`	`23 bp deleted → frameshift`
90	`TCTGGATGGGGGTCAGCTTGGCACTCTG------------------------TCCGAAGC`	`24 bp deleted → no frameshift`
81	`TCTGGATGGGGGTCAGCT--------------------------CTAATTTGTCCGAAGC`	`26 bp deleted → frameshift`
80	`TCTGGATGGGGGTCAGCTTG--------------------------------TCCGAAGC`	`32 bp deleted → frameshift`
80	`TCTGGATGG--------------------------------TCTCTAATTTGTCCGAAGC`	`32 bp deleted → frameshift`
74	`TCTGGATGGGGGTCAGCTTGGCACTC----------------------------CGAAGC`	`28 bp deleted → frameshift`
74	`TCTGGATGGGGGTCAGCT----------------------------AATTTGTCCGAAGC`	`28 bp deleted → frameshift`
69	`TCTGG-----------------------------------GTCTCTAATTTGTCCGAAGC`	`35 bp deleted → frameshift`
67	`TCTGGATGGGGGTC----------------------------------------CGAAGC`	`40 bp deleted → frameshift`
66	`TCTGG------------------------------------TCTCTAATTTGTCCGAAGC`	`36 bp deleted → no frameshift`
63	`TCTGGATGGGGGTC-------------------------------TAATTTGTCCGAAGC`	`31 bp deleted → frameshift`
60	`TCTGGA--------------------------------GGGTCTCTAATTTGTCCGAAGC`	`32 bp deleted → frameshift`
60	`TCTGGATGGGGGTCAGCTTGGC--------------------------------------`	`38 bp deleted → frameshift`
58	`TCTGGATGGGGGTCAGC-------------------------------------------`	`43 bp deleted → frameshift`
51	`TCT-----------------------------------------CTAATTTGTCCGAAGC`	`41 bp deleted → frameshift`
46	`TCT-------------------------------------------AATTTGTCCGAAGC`	`43 bp deleted → frameshift`
42	`TCTGGATGGGGGTCAGCTT-------------------------------TGTCCGAAGC`	`31 bp deleted → frameshift`
33	`TCTGGATG--------------------------------------------TCCGAAGC`	`44 bp deleted → frameshift`
27	`TCTG------------------------------------------------TCCGAAGC`	`48 bp deleted → no frameshift`
24	`TCTGGAT------------------------------------------TTGTCCGAAGC`	`42 bp deleted → no frameshift`
23	`TCTGGA---------------------------------------------------AGC`	`51 bp deleted → no frameshift`
22	`TC----------------------------------------------------CGAAGC`	`52 bp deleted → frameshift`

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