Position 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 Variants ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Sequence ctcctttcatcttcactgtggcagacgtttctatttatccacttgcgttcgccgagtggcgtcaccagcggtactgtaatgacgattgcagcaggaggatgacagcttagaaagaagagggcaatggggcttcctcccagaggcggtgcggcacagaggagcgctcgcatcacaaggtgaccctagctccccactgccatctccgcggtcgccgtcgacacggcgctggggctacccggcgcctgccttgtcgccttagctcctcttctcagccaagatcccagggagcctgggattaggagcttacttgggggttttccccctccccccctctggagagtccggggATGgagagccgaaaggacatggttatgtttctggatgggggtcagcttggcactctggttggtaagagggtctctaatttgtccgaagccgtgagcagcccgctgcctgaaccgccagagaagatggtgccccacggttgcctgagcccgcgagccggccctccgacttcccgggagcgtggcgggggaggccaggaggaggagccggtcgatggactagcaggcagtgctgcagggctgggcgccgagccacggtctgctggagcggccatgcttggcccgggacccccagtcccctccgcggacagcctctctggccaagggcaacctagtagctcagacaccgaatcggatttctatgaagaaatcgaggtgagctgcaccccagactgcgccaccgggaacgccgagtaccagcacagcaaag CCT--- CCA--- CCG--- CCA--- −−−AGG −−−AGG −−−GGG CCT--- −−−CGG −−−AGG −−−AGG CCC--- CCC--- CCA--- CCG--- −−−TGG CCC--- CCT--- CCT--- CCT--- CCA--- CCA--- CCT--- −−−TGG CCC--- CCC--- CCG--- CCG--- −−−TGG −−−TGG −−−TGG −−−TGG −−−AGG CCG--- CCC--- CCG--- −−−TGG CCC--- CCT--- CCG--- CCG--- CCG--- −−−CGG CCA--- CCG--- −−−AGG −−−AGG CCG--- −−−TGG CCA--- CCC--- CCC--- CCC--- CCT--- CCA--- CCT--- CCG--- −−−AGG CCC--- CCA--- −−−TGG −−−CGG −−−AGG −−−GGG −−−GGG CCC--- −−−CGG CCT--- CCC--- CCG--- −−−CGG −−−GGG CCG--- CCC--- −−−TGG −−−GGG CCC--- CCC--- −−−CGG −−−AGG −−−TGG −−−TGG −−−GGG CCG--- CCG--- CCA--- CCC--- CCC--- CCC--- CCC--- −−−GGG −−−AGG −−−CGG −−−GGG CCA--- −−−CGG −−−TGG CCC--- CCC--- −−−TGG −−−CGG CCC--- CCG--- −−−TGG CCA--- CCA--- −−−CGG −−−GGG −−−CGG CCT--- −−−AGG −−−GGG −−−GGG CCC--- CCC--- −−−GGG −−−GGG CCT--- CCA--- CCG--- CCT--- −−−CGG −−−TGG −−−AGG −−−AGG −−−TGG −−−TGG −−−CGG CCG--- CCC--- CCT--- −−−AGG CCA--- −−−CGG −−−AGG −−−GGG −−−AGG −−−GGG CCT--- CCT--- −−−GGG −−−GGG −−−CGG −−−CGG −−−GGG −−−GGG −−−AGG −−−GGG −−−CGG CCA--- CCG--- −−−GGG −−−GGG CCC--- −−−TGG −−−GGG −−−GGG −−−GGG −−−CGG CCC--- −−−TGG
Position/ Strand |
Guide Sequence + PAM + Restriction Enzymes + Variants Only G- Only GG- Only A- | MIT Specificity Score | CFD Spec. score | Predicted Efficiency
Show all scores |
Outcome | Off-targets for 0-1-2-3-4 mismatches + next to PAM |
Genome Browser links to matches sorted by CFD off-target score
exons only chr6 only |
||
---|---|---|---|---|---|---|---|---|---|
39 / rev |
CGCCACTCGGCGAACGCAAG TGG
.................... ... Cloning / PCR primers |
99 | 99 | 48 | 52 | 72 | 80 |
0 - 0 - 0 - 0 - 4
0 - 0 - 0 - 0 - 0 4 off-targets |
show all... show less... |
57 / fw |
ATCCACTTGCGTTCGCCGAG TGG
.................... ... Enzymes: NmeAIII, AcyI
Cloning / PCR primers
|
99 | 99 | 61 | 33 | 81 | 87 |
0 - 0 - 0 - 1 - 7
0 - 0 - 0 - 0 - 0 8 off-targets |
show all... show less... |
221 / fw |
CTCCGCGGTCGCCGTCGACA CGG
.................... ... Enzymes: BfoI, HinP1I, Hpy166II, TaqI, BceAI, HincII, SalI, FblI
Cloning / PCR primers
|
99 | 98 | 47 | 42 | 64 | 76 |
0 - 0 - 0 - 1 - 14
0 - 0 - 0 - 0 - 0 15 off-targets |
show all... show less... |
65 / rev |
AATCGTCATTACAGTACCGC TGG
.................... ... Enzymes: AsuHPI, MspA1I, SsiI, MaeIII, Tsp45I
Cloning / PCR primers
|
98 | 98 | 57 | 28 | 57 | 74 |
0 - 0 - 0 - 0 - 20
0 - 0 - 0 - 0 - 0 20 off-targets |
show all... show less... |
212 / rev |
TAGCCCCAGCGCCGTGTCGA CGG
.................... ... Enzymes: BceAI, Hpy166II, Hpy99I, HincII, TaqI, SalI, FblI
Cloning / PCR primers
|
97 | 97 | 48 | 59 | 52 | 84 |
0 - 0 - 0 - 1 - 27
0 - 0 - 0 - 0 - 0 28 off-targets |
show all... show less... |
228 / fw |
GTCGCCGTCGACACGGCGCT GGG
.................... ... Enzymes: HinP1I, BfoI
Cloning / PCR primers
|
97 | 98 | 39 | 53 | 60 | 88 |
0 - 0 - 0 - 1 - 11
0 - 0 - 0 - 0 - 0 12 off-targets |
show all... show less... |
229 / fw |
TCGCCGTCGACACGGCGCTG GGG
.................... ... Enzymes: HinP1I, BfoI
Cloning / PCR primers
|
97 | 99 | 63 | 70 | 65 | 85 |
0 - 0 - 0 - 1 - 14
0 - 0 - 0 - 1 - 0 15 off-targets |
show all... show less... |
732 / rev |
GCGTTCCCGGTGGCGCAGTC TGG
.................... ... Cloning / PCR primers |
97 | 99 | 26 | 64 | 60 | 82 |
0 - 0 - 0 - 1 - 19
0 - 0 - 0 - 0 - 0 20 off-targets |
show all... show less... |
52 / rev |
GTACCGCTGGTGACGCCACT CGG
.................... ... Cloning / PCR primers |
96 | 97 | 56 | 45 | 81 | 88 |
0 - 0 - 0 - 2 - 25
0 - 0 - 0 - 0 - 0 27 off-targets |
show all... show less... |
203 / rev |
CGCCGTGTCGACGGCGACCG CGG
.................... ... High GC content Enzymes: BstDSI, SacII, BspFNI, MspA1I, BseDI, Bsh1285I, SsiI
Cloning / PCR primers
|
96 | 99 | 52 | 67 | 51 | 66 |
0 - 0 - 0 - 1 - 16
0 - 0 - 0 - 0 - 0 17 off-targets |
show all... show less... |
227 / fw |
GGTCGCCGTCGACACGGCGC TGG
.................... ... High GC content Enzymes: HinP1I, BceAI, BfoI
Cloning / PCR primers
|
96 | 98 | 30 | 24 | 60 | 71 |
0 - 0 - 0 - 2 - 14
0 - 0 - 0 - 0 - 0 16 off-targets |
show all... show less... |
478 / rev |
TCGCGGGCTCAGGCAACCGT GGG
.................... ... Enzymes: BstDSI, BaeGI, NlaIV, BseDI, BanI, Bsp1286I, HpyCH4III
Cloning / PCR primers
|
96 | 98 | 46 | 74 | 68 | 85 |
0 - 0 - 0 - 1 - 23
0 - 0 - 0 - 0 - 0 24 off-targets |
show all... show less... |
742 / rev |
CTGGTACTCGGCGTTCCCGG TGG
.................... ... Enzymes: MspI, HinP1I, LpnPI, StyD4I, NciI
Cloning / PCR primers
|
96 | 97 | 65 | 74 | 52 | 64 |
0 - 0 - 0 - 2 - 19
0 - 0 - 0 - 0 - 0 21 off-targets |
show all... show less... |
69 / fw |
TCGCCGAGTGGCGTCACCAG CGG
.................... ... Enzymes: Csp6I, LpnPI, MspA1I, MwoI
Cloning / PCR primers
|
95 | 98 | 61 | 98 | 57 | 54 |
0 - 0 - 0 - 1 - 20
0 - 0 - 0 - 1 - 0 21 off-targets |
show all... show less... |
569 / fw |
GAGCCGGTCGATGGACTAGC AGG
.................... ... Enzymes: BstC8I, BtsIMutI, BtsI, MaeI
Cloning / PCR primers
|
95 | 96 | 51 | 67 | 62 | 79 |
0 - 0 - 0 - 3 - 21
0 - 0 - 0 - 0 - 0 24 off-targets |
show all... show less... |
731 / rev |
CGTTCCCGGTGGCGCAGTCT GGG
.................... ... Enzymes: HpyCH4V
Cloning / PCR primers
|
95 | 98 | 34 | 60 | 61 | 65 |
0 - 0 - 1 - 4 - 22
0 - 0 - 0 - 2 - 0 27 off-targets |
show all... show less... |
745 / rev |
GTGCTGGTACTCGGCGTTCC CGG
.................... ... Inefficient Enzymes: MspI, NciI, LpnPI, StyD4I
Cloning / PCR primers
|
95 | 97 | 39 | 72 | 55 | 72 |
0 - 0 - 0 - 0 - 54
0 - 0 - 0 - 0 - 0 54 off-targets |
show all... show less... |
93 / fw |
ACTGTAATGACGATTGCAGC AGG
.................... ... Enzymes: EcoP15I, BstV1I, ApeKI, Fsp4HI, HpyCH4V
Cloning / PCR primers
|
94 | 96 | 57 | 35 | 70 | 79 |
0 - 0 - 0 - 2 - 44
0 - 0 - 0 - 1 - 0 46 off-targets |
show all... show less... |
494 / rev |
AAGTCGGAGGGCCGGCTCGC GGG
.................... ... Enzymes: BspFNI, BspCNI, BstDEI, BslI, Bsp1286I, Eco24I, BstC8I
Cloning / PCR primers
|
94 | 97 | 42 | 61 | 70 | 85 |
0 - 0 - 0 - 2 - 19
0 - 0 - 0 - 0 - 0 21 off-targets |
show all... show less... |
697 / fw |
TAGTAGCTCAGACACCGAAT CGG
.................... ... Enzymes: HinfI, Hpy188I, PfeI
Cloning / PCR primers
|
94 | 95 | 63 | 57 | 55 | 72 |
0 - 0 - 0 - 5 - 54
0 - 0 - 0 - 0 - 0 59 off-targets |
show all... show less... |
479 / rev |
CTCGCGGGCTCAGGCAACCG TGG
.................... ... Enzymes: Bsp1286I, BstDSI, BaeGI, HpyCH4III, BseDI
Cloning / PCR primers
|
93 | 97 | 52 | 54 | 67 | 82 |
0 - 0 - 0 - 1 - 42
0 - 0 - 0 - 0 - 0 43 off-targets |
show all... show less... |
503 / fw |
GGTTGCCTGAGCCCGCGAGC CGG
.................... ... Enzymes: BshFI, MnlI, MspI, PspPI, LpnPI, BslI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
93 | 95 | 53 | 39 | 67 | 82 |
0 - 0 - 0 - 3 - 53
0 - 0 - 0 - 0 - 0 56 off-targets |
show all... show less... |
532 / fw |
GACTTCCCGGGAGCGTGGCG GGG
.................... ... Enzymes: MwoI
Cloning / PCR primers
|
93 | 95 | 44 | 55 | 65 | 85 |
0 - 0 - 0 - 3 - 47
0 - 0 - 0 - 0 - 1 50 off-targets |
show all... show less... |
552 / rev |
CTGCCTGCTAGTCCATCGAC CGG
.................... ... Enzymes: NlaIV, MspI, BslI, TaqI, LpnPI, Bsh1285I, LmnI, Cfr10I
Cloning / PCR primers
|
93 | 94 | 69 | 58 | 72 | 79 |
0 - 0 - 0 - 4 - 61
0 - 0 - 0 - 0 - 0 65 off-targets |
show all... show less... |
190 / rev |
GCGACCGCGGAGATGGCAGT GGG
.................... ... Enzymes: AluBI, BtsIMutI, BtsI, TspRI
Cloning / PCR primers
|
92 | 97 | 56 | 64 | 63 | 75 |
0 - 0 - 0 - 3 - 34
0 - 0 - 0 - 1 - 0 37 off-targets |
show all... show less... |
197 / rev |
GTCGACGGCGACCGCGGAGA TGG
.................... ... Enzymes: MwoI, BtsIMutI, BtsI
Cloning / PCR primers
|
92 | 97 | 51 | 70 | 48 | 60 |
0 - 0 - 0 - 4 - 31
0 - 0 - 0 - 1 - 1 35 off-targets |
show all... show less... |
477 / rev |
CGCGGGCTCAGGCAACCGTG GGG
.................... ... Enzymes: BstDSI, BaeGI, NlaIV, BseDI, BanI, Bsp1286I, HpyCH4III
Cloning / PCR primers
|
92 | 96 | 64 | 79 | 74 | 88 |
0 - 0 - 1 - 4 - 42
0 - 0 - 0 - 0 - 0 47 off-targets |
show all... show less... |
506 / rev |
CACGCTCCCGGGAAGTCGGA GGG
.................... ... Enzymes: BshFI, MspI, Hpy188I, PspPI, LpnPI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
92 | 95 | 54 | 56 | 65 | 78 |
0 - 0 - 0 - 6 - 33
0 - 0 - 0 - 0 - 0 39 off-targets |
show all... show less... |
510 / rev |
CCGCCACGCTCCCGGGAAGT CGG
.................... ... Enzymes: Hpy188I
Cloning / PCR primers
|
92 | 97 | 43 | 74 | 68 | 82 |
0 - 0 - 0 - 1 - 41
0 - 0 - 0 - 0 - 1 42 off-targets |
show all... show less... |
597 / rev |
ATGGCCGCTCCAGCAGACCG TGG
.................... ... Enzymes: BstDSI, BslI, HpyCH4III, BseDI
Cloning / PCR primers
|
92 | 96 | 63 | 49 | 66 | 84 |
0 - 0 - 1 - 0 - 42
0 - 0 - 0 - 0 - 2 43 off-targets |
show all... show less... |
495 / rev |
GAAGTCGGAGGGCCGGCTCG CGG
.................... ... Enzymes: BspFNI, Bsp1286I, BslI, Eco24I, BstC8I
Cloning / PCR primers
|
91 | 94 | 49 | 54 | 71 | 80 |
0 - 0 - 0 - 4 - 47
0 - 0 - 0 - 0 - 0 51 off-targets |
show all... show less... |
531 / fw |
CGACTTCCCGGGAGCGTGGC GGG
.................... ... Cloning / PCR primers |
91 | 96 | 34 | 43 | 64 | 91 |
0 - 0 - 0 - 3 - 37
0 - 0 - 0 - 0 - 0 40 off-targets |
show all... show less... |
533 / fw |
ACTTCCCGGGAGCGTGGCGG GGG
.................... ... Enzymes: BshFI, MwoI
Cloning / PCR primers
|
91 | 94 | 46 | 81 | 62 | 79 |
0 - 0 - 0 - 3 - 71
0 - 0 - 0 - 0 - 1 74 off-targets |
show all... show less... |
642 / rev |
CCAGAGAGGCTGTCCGCGGA GGG
.................... ... Enzymes: BstDSI, BslFI, SacII, BspFNI, MspA1I, BseDI, BslI
Cloning / PCR primers
|
91 | 96 | 47 | 63 | 68 | 76 |
0 - 0 - 0 - 5 - 87
0 - 0 - 0 - 0 - 0 92 off-targets |
show all... show less... |
133 / rev |
TGTGCCGCACCGCCTCTGGG AGG
.................... ... Enzymes: BstENI, BslI
Cloning / PCR primers
|
90 | 94 | 62 | 51 | 67 | 83 |
0 - 0 - 0 - 8 - 48
0 - 0 - 0 - 2 - 1 56 off-targets |
show all... show less... |
430 / rev |
CAGCGGGCTGCTCACGGCTT CGG
.................... ... Inefficient Enzymes: BceAI, Hpy188I
Cloning / PCR primers
|
90 | 95 | 42 | 80 | 61 | 67 |
0 - 0 - 1 - 4 - 54
0 - 0 - 0 - 0 - 0 59 off-targets |
show all... show less... |
519 / fw |
GAGCCGGCCCTCCGACTTCC CGG
.................... ... Inefficient Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, Cfr9I
Cloning / PCR primers
|
90 | 92 | 39 | 51 | 77 | 85 |
0 - 0 - 1 - 5 - 67
0 - 0 - 0 - 0 - 0 73 off-targets |
show all... show less... |
530 / fw |
CCGACTTCCCGGGAGCGTGG CGG
.................... ... Cloning / PCR primers |
90 | 92 | 56 | 66 | 67 | 85 |
0 - 0 - 0 - 3 - 54
0 - 0 - 0 - 0 - 2 57 off-targets |
show all... show less... |
643 / rev |
GCCAGAGAGGCTGTCCGCGG AGG
.................... ... Enzymes: BstDSI, BslFI, SacII, BspFNI, MspA1I, BseDI, BslI, SsiI
Cloning / PCR primers
|
90 | 91 | 47 | 79 | 68 | 81 |
0 - 0 - 1 - 8 - 77
0 - 0 - 0 - 0 - 0 86 off-targets |
show all... show less... |
691 / rev |
TTCTTCATAGAAATCCGATT CGG
.................... ... Enzymes: HinfI, Hpy188I, PfeI
Cloning / PCR primers
|
90 | 94 | 45 | 43 | 54 | 81 |
0 - 0 - 1 - 8 - 90
0 - 0 - 0 - 1 - 0 99 off-targets |
show all... show less... |
746 / fw |
TGCACCCCAGACTGCGCCAC CGG
.................... ... Enzymes: MspI, NciI, LpnPI, StyD4I
Cloning / PCR primers
|
90 | 97 | 44 | 63 | 57 | 63 |
0 - 0 - 1 - 4 - 88
0 - 0 - 0 - 0 - 0 93 off-targets |
show all... show less... |
137 / rev |
CCTCTGTGCCGCACCGCCTC TGG
.................... ... Enzymes: BstENI, BslI, MnlI
Cloning / PCR primers
|
89 | 96 | 30 | 49 | 71 | 72 |
0 - 0 - 0 - 1 - 107
0 - 0 - 0 - 0 - 0 108 off-targets |
show all... show less... |
236 / rev |
TAAGGCGACAAGGCAGGCGC CGG
.................... ... Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I, BstC8I
Cloning / PCR primers
|
89 | 92 | 44 | 47 | 63 | 72 |
0 - 0 - 1 - 7 - 54
0 - 0 - 0 - 2 - 0 62 off-targets |
show all... show less... |
527 / fw |
CCTCCGACTTCCCGGGAGCG TGG
.................... ... Cloning / PCR primers |
89 | 93 | 56 | 38 | 71 | 85 |
0 - 0 - 0 - 7 - 64
0 - 0 - 0 - 0 - 2 71 off-targets |
show all... show less... |
592 / rev |
CGCTCCAGCAGACCGTGGCT CGG
.................... ... Enzymes: BstDSI, NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BslI, SspDI
Cloning / PCR primers
|
89 | 93 | 43 | 44 | 66 | 75 |
0 - 0 - 3 - 3 - 65
0 - 0 - 0 - 0 - 0 71 off-targets |
show all... show less... |
675 / rev |
GATTCGGTGTCTGAGCTACT AGG
.................... ... Enzymes: MaeI
Cloning / PCR primers
|
89 | 95 | 56 | 58 | 72 | 87 |
0 - 0 - 0 - 6 - 41
0 - 0 - 0 - 1 - 1 47 off-targets |
show all... show less... |
136 / rev |
CTCTGTGCCGCACCGCCTCT GGG
.................... ... Inefficient Enzymes: BstENI, BslI, MnlI
Cloning / PCR primers
|
88 | 90 | 35 | 40 | 68 | 79 |
0 - 0 - 0 - 4 - 113
0 - 0 - 0 - 0 - 0 117 off-targets |
show all... show less... |
175 / fw |
AGAGGAGCGCTCGCATCACA AGG
.................... ... Enzymes: AsuHPI, BstEII, DraIII, MaeIII, Tsp45I
Cloning / PCR primers
|
88 | 90 | 61 | 51 | 64 | 78 |
0 - 0 - 0 - 8 - 86
0 - 0 - 0 - 0 - 0 94 off-targets |
show all... show less... |
507 / rev |
CCACGCTCCCGGGAAGTCGG AGG
.................... ... Enzymes: MspI, BshFI, LpnPI, Hpy188I, PspPI
Cloning / PCR primers
|
88 | 93 | 53 | 49 | 63 | 88 |
0 - 0 - 0 - 3 - 46
0 - 0 - 0 - 0 - 0 49 off-targets |
show all... show less... |
608 / fw |
GGCGCCGAGCCACGGTCTGC TGG
.................... ... High GC content Enzymes: BpmI
Cloning / PCR primers
|
87 | 94 | 34 | 15 | 62 | 80 |
0 - 0 - 1 - 5 - 41
0 - 0 - 0 - 0 - 1 47 off-targets |
show all... show less... |
361 / fw |
TCCGGGGATGGAGAGCCGAA AGG
.................... ... Enzymes: NlaIII
Cloning / PCR primers
|
86 | 91 | 63 | 61 | 55 | 83 |
0 - 0 - 0 - 3 - 87
0 - 0 - 0 - 0 - 1 90 off-targets |
show all... show less... |
560 / fw |
CAGGAGGAGGAGCCGGTCGA TGG
.................... ... Enzymes: Bsh1285I, BslI, TaqI, MaeI
Cloning / PCR primers
|
86 | 87 | 50 | 65 | 81 | 87 |
0 - 0 - 0 - 29 - 298
0 - 0 - 0 - 3 - 0 327 off-targets |
show all... show less... |
189 / rev |
CGACCGCGGAGATGGCAGTG GGG
.................... ... Enzymes: AluBI, BtsIMutI, BtsI, TspRI
Cloning / PCR primers
|
85 | 93 | 73 | 60 | 68 | 88 |
0 - 0 - 0 - 4 - 58
0 - 0 - 0 - 2 - 4 62 off-targets |
show all... show less... |
453 / rev |
ACCATCTTCTCTGGCGGTTC AGG
.................... ... Inefficient Enzymes: MwoI, ApeKI, Fsp4HI, BstV1I
Cloning / PCR primers
|
85 | 94 | 29 | 53 | 54 | 60 |
0 - 0 - 1 - 7 - 88
0 - 0 - 0 - 1 - 1 96 off-targets |
show all... show less... |
520 / fw |
AGCCGGCCCTCCGACTTCCC GGG
.................... ... Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, Cfr9I
Cloning / PCR primers
|
85 | 92 | 51 | 45 | 75 | 82 |
0 - 0 - 1 - 6 - 85
0 - 0 - 1 - 1 - 0 92 off-targets |
show all... show less... |
309 / fw |
CCTGGGATTAGGAGCTTACT TGG
.................... ... Cloning / PCR primers |
84 | 88 | 28 | 40 | 64 | 85 |
0 - 0 - 0 - 11 - 131
0 - 0 - 0 - 0 - 1 142 off-targets |
show all... show less... |
311 / fw |
TGGGATTAGGAGCTTACTTG GGG
.................... ... Inefficient Cloning / PCR primers |
84 | 94 | 53 | 44 | 64 | 74 |
0 - 0 - 0 - 12 - 83
0 - 0 - 0 - 3 - 0 95 off-targets |
show all... show less... |
312 / fw |
GGGATTAGGAGCTTACTTGG GGG
.................... ... Cloning / PCR primers |
84 | 88 | 61 | 67 | 67 | 84 |
0 - 0 - 0 - 9 - 94
0 - 0 - 0 - 0 - 2 103 off-targets |
show all... show less... |
628 / fw |
TGGAGCGGCCATGCTTGGCC CGG
.................... ... Inefficient Enzymes: BshFI, BmeT110I, NlaIV, BslFI, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
Cloning / PCR primers
|
84 | 88 | 36 | 62 | 64 | 82 |
0 - 0 - 0 - 11 - 92
0 - 0 - 0 - 1 - 5 103 off-targets |
show all... show less... |
237 / fw |
GACACGGCGCTGGGGCTACC CGG
.................... ... Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I
Cloning / PCR primers
|
83 | 92 | 48 | 47 | 69 | 88 |
0 - 0 - 1 - 2 - 82
0 - 0 - 0 - 1 - 3 85 off-targets |
show all... show less... |
414 / fw |
TTGGCACTCTGGTTGGTAAG AGG
.................... ... Enzymes: BspTNI, BcoDI
Cloning / PCR primers
|
83 | 90 | 54 | 56 | 80 | 85 |
0 - 0 - 0 - 5 - 102
0 - 0 - 0 - 0 - 3 107 off-targets |
show all... show less... |
502 / rev |
CTCCCGGGAAGTCGGAGGGC CGG
.................... ... Enzymes: BshFI, MspI, PspPI, LpnPI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
83 | 88 | 50 | 51 | 69 | 76 |
0 - 0 - 0 - 15 - 91
0 - 0 - 0 - 0 - 1 106 off-targets |
show all... show less... |
600 / fw |
CAGGGCTGGGCGCCGAGCCA CGG
.................... ... High GC content Enzymes: BstDSI, BslI, HpyCH4III, BseDI
Cloning / PCR primers
|
82 | 87 | 42 | 70 | 66 | 76 |
0 - 0 - 0 - 16 - 170
0 - 0 - 0 - 0 - 0 186 off-targets |
show all... show less... |
662 / fw |
CCCTCCGCGGACAGCCTCTC TGG
.................... ... Inefficient Enzymes: BshFI, BalI, MwoI, MnlI, BglI, AcoI
Cloning / PCR primers
|
82 | 90 | 35 | 43 | 70 | 78 |
0 - 0 - 0 - 8 - 72
0 - 0 - 0 - 1 - 0 80 off-targets |
show all... show less... |
747 / fw |
GCACCCCAGACTGCGCCACC GGG
.................... ... Enzymes: MspI, NciI, LpnPI, StyD4I
Cloning / PCR primers
|
82 | 91 | 58 | 48 | 60 | 77 |
0 - 0 - 0 - 10 - 108
0 - 0 - 0 - 0 - 1 118 off-targets |
show all... show less... |
96 / fw |
GTAATGACGATTGCAGCAGG AGG
.................... ... Enzymes: EcoP15I, BtsCI
Cloning / PCR primers
|
81 | 93 | 65 | 75 | 70 | 78 |
0 - 0 - 0 - 12 - 56
0 - 0 - 0 - 2 - 5 68 off-targets |
show all... show less... |
310 / fw |
CTGGGATTAGGAGCTTACTT GGG
.................... ... Inefficient Cloning / PCR primers |
81 | 89 | 38 | 43 | 65 | 82 |
0 - 0 - 0 - 9 - 109
0 - 0 - 0 - 1 - 3 118 off-targets |
show all... show less... |
488 / rev |
GAGGGCCGGCTCGCGGGCTC AGG
.................... ... High GC content Enzymes: Bsp1286I, BstDEI, BstC8I, Eco24I, BspCNI
Cloning / PCR primers
|
81 | 93 | 36 | 80 | 64 | 73 |
0 - 0 - 1 - 8 - 76
0 - 0 - 0 - 2 - 0 85 off-targets |
show all... show less... |
665 / rev |
CTGAGCTACTAGGTTGCCCT TGG
.................... ... Enzymes: BshFI, BalI, BseDI, ErhI, LpnPI, AcoI
Cloning / PCR primers
|
81 | 88 | 51 | 50 | 65 | 78 |
0 - 0 - 2 - 13 - 122
0 - 0 - 0 - 1 - 0 137 off-targets |
show all... show less... |
157 / fw |
CCAGAGGCGGTGCGGCACAG AGG
.................... ... Enzymes: HinP1I, BseRI
Cloning / PCR primers
|
80 | 88 | 59 | 72 | 70 | 88 |
0 - 0 - 0 - 10 - 133
0 - 0 - 0 - 0 - 0 143 off-targets |
show all... show less... |
235 / rev |
AAGGCGACAAGGCAGGCGCC GGG
.................... ... Enzymes: NlaIV, AcyI, NciI, MspI, BfoI, HinP1I, BanI, LpnPI, SspDI, StyD4I
Cloning / PCR primers
|
80 | 90 | 52 | 70 | 68 | 70 |
0 - 0 - 1 - 12 - 93
0 - 0 - 0 - 0 - 0 106 off-targets |
show all... show less... |
242 / rev |
AGGAGCTAAGGCGACAAGGC AGG
.................... ... Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI, BstC8I
Cloning / PCR primers
|
80 | 88 | 49 | 42 | 64 | 72 |
0 - 0 - 1 - 13 - 149
0 - 0 - 0 - 0 - 0 163 off-targets |
show all... show less... |
246 / rev |
GAAGAGGAGCTAAGGCGACA AGG
.................... ... Enzymes: BstC8I
Cloning / PCR primers
|
80 | 82 | 61 | 37 | 65 | 81 |
0 - 0 - 1 - 17 - 201
0 - 0 - 0 - 2 - 1 219 off-targets |
show all... show less... |
613 / fw |
CGAGCCACGGTCTGCTGGAG CGG
.................... ... Enzymes: BshFI, TauI, MwoI, BpmI, NlaIII, AcoI, Fsp4HI
Cloning / PCR primers
|
79 | 91 | 49 | 62 | 68 | 77 |
0 - 0 - 1 - 10 - 58
0 - 0 - 0 - 3 - 10 69 off-targets |
show all... show less... |
636 / rev |
AGGCTGTCCGCGGAGGGGAC TGG
.................... ... Enzymes: BmuI, Bse1I, Eam1105I, BslFI, BslI
Cloning / PCR primers
|
79 | 88 | 30 | 59 | 69 | 76 |
0 - 0 - 2 - 6 - 99
0 - 0 - 0 - 0 - 1 107 off-targets |
show all... show less... |
649 / fw |
GGGACCCCCAGTCCCCTCCG CGG
.................... ... High GC content Enzymes: BstDSI, SacII, MnlI, BspFNI, MspA1I, BseDI, BslI, SsiI
Cloning / PCR primers
|
79 | 88 | 68 | 70 | 68 | 81 |
0 - 0 - 1 - 9 - 104
0 - 0 - 0 - 1 - 1 114 off-targets |
show all... show less... |
415 / fw |
TGGCACTCTGGTTGGTAAGA GGG
.................... ... Enzymes: BspTNI, BcoDI
Cloning / PCR primers
|
78 | 84 | 45 | 56 | 77 | 79 |
0 - 0 - 1 - 13 - 125
0 - 0 - 0 - 2 - 3 139 off-targets |
show all... show less... |
518 / rev |
GGCCTCCCCCGCCACGCTCC CGG
.................... ... High GC content Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, LmnI, Cfr9I
Cloning / PCR primers
|
78 | 88 | 32 | 37 | 71 | 77 |
0 - 0 - 3 - 13 - 134
0 - 0 - 0 - 2 - 2 150 off-targets |
show all... show less... |
616 / rev |
TGGGGGTCCCGGGCCAAGCA TGG
.................... ... Enzymes: BshFI, TauI, NlaIII, AcoI, SsiI, Fsp4HI
Cloning / PCR primers
|
78 | 87 | 45 | 53 | 72 | 89 |
0 - 0 - 0 - 13 - 128
0 - 0 - 0 - 1 - 0 141 off-targets |
show all... show less... |
191 / rev |
GGCGACCGCGGAGATGGCAG TGG
.................... ... Enzymes: MwoI, BtsIMutI, BtsI, TspRI
Cloning / PCR primers
|
77 | 88 | 46 | 48 | 64 | 69 |
0 - 0 - 0 - 10 - 105
0 - 0 - 0 - 1 - 1 115 off-targets |
show all... show less... |
633 / rev |
CTGTCCGCGGAGGGGACTGG GGG
.................... ... Enzymes: Bse1I, NlaIV, Eam1105I, EcoO109I, PpuMI, PspPI, Bme18I, BmuI, KflI
Cloning / PCR primers
|
77 | 86 | 44 | 67 | 65 | 78 |
0 - 0 - 0 - 12 - 107
0 - 0 - 0 - 6 - 5 119 off-targets |
show all... show less... |
646 / rev |
TTGGCCAGAGAGGCTGTCCG CGG
.................... ... Enzymes: BstDSI, SacII, BspFNI, MspA1I, BseDI, BslI, SsiI
Cloning / PCR primers
|
77 | 86 | 63 | 70 | 68 | 87 |
0 - 0 - 0 - 17 - 130
0 - 0 - 0 - 0 - 2 147 off-targets |
show all... show less... |
149 / fw |
GCTTCCTCCCAGAGGCGGTG CGG
.................... ... Enzymes: TauI, Fsp4HI, BslI
Cloning / PCR primers
|
76 | 80 | 61 | 61 | 71 | 85 |
0 - 0 - 3 - 24 - 165
0 - 0 - 0 - 2 - 1 192 off-targets |
show all... show less... |
436 / rev |
TTCAGGCAGCGGGCTGCTCA CGG
.................... ... Enzymes: BceAI
Cloning / PCR primers
|
76 | 85 | 52 | 51 | 82 | 88 |
0 - 0 - 0 - 16 - 107
0 - 0 - 0 - 0 - 2 123 off-targets |
show all... show less... |
462 / rev |
CCGTGGGGCACCATCTTCTC TGG
.................... ... Inefficient Cloning / PCR primers |
76 | 93 | 39 | 57 | 66 | 67 |
0 - 0 - 2 - 22 - 81
0 - 0 - 0 - 2 - 2 105 off-targets |
show all... show less... |
623 / fw |
TCTGCTGGAGCGGCCATGCT TGG
.................... ... Enzymes: BshFI, MspI, PspPI, NciI, NlaIII, LpnPI, StyD4I
Cloning / PCR primers
|
76 | 89 | 43 | 50 | 68 | 90 |
0 - 0 - 1 - 11 - 112
0 - 0 - 0 - 0 - 3 124 off-targets |
show all... show less... |
634 / rev |
GCTGTCCGCGGAGGGGACTG GGG
.................... ... Enzymes: Bse1I, NlaIV, Eam1105I, BslFI, EcoO109I, PpuMI, PspPI, Bme18I, BmuI, KflI
Cloning / PCR primers
|
75 | 86 | 39 | 64 | 66 | 81 |
0 - 0 - 1 - 9 - 105
0 - 0 - 0 - 1 - 1 115 off-targets |
show all... show less... |
730 / rev |
GTTCCCGGTGGCGCAGTCTG GGG
.................... ... Enzymes: BsgI, HpyCH4V
Cloning / PCR primers
|
75 | 83 | 58 | 75 | 57 | 73 |
0 - 0 - 1 - 6 - 130
0 - 0 - 0 - 0 - 0 137 off-targets |
show all... show less... |
289 / rev |
CCAAGTAAGCTCCTAATCCC AGG
.................... ... Enzymes: NlaIV, BseDI, LpnPI, BstNI, LmnI, StyD4I
Cloning / PCR primers
|
74 | 92 | 54 | 40 | 70 | 79 |
0 - 0 - 0 - 7 - 109
0 - 0 - 0 - 0 - 0 116 off-targets |
show all... show less... |
447 / rev |
TTCTCTGGCGGTTCAGGCAG CGG
.................... ... Enzymes: MwoI, MspA1I, ApeKI, BstV1I, Fsp4HI, BstC8I
Cloning / PCR primers
|
74 | 81 | 39 | 54 | 67 | 82 |
0 - 0 - 1 - 15 - 147
0 - 0 - 0 - 1 - 0 163 off-targets |
show all... show less... |
635 / rev |
GGCTGTCCGCGGAGGGGACT GGG
.................... ... Enzymes: Bse1I, NlaIV, Eam1105I, BslFI, PspPI, Bme18I, BmuI
Cloning / PCR primers
|
74 | 88 | 25 | 58 | 67 | 77 |
0 - 0 - 0 - 14 - 97
0 - 0 - 0 - 0 - 1 111 off-targets |
show all... show less... |
403 / fw |
TGGGGGTCAGCTTGGCACTC TGG
.................... ... Cloning / PCR primers |
73 | 86 | 41 | 59 | 69 | 72 |
0 - 0 - 3 - 13 - 139
0 - 0 - 0 - 1 - 2 155 off-targets |
show all... show less... |
718 / fw |
GGATTTCTATGAAGAAATCG AGG
.................... ... Enzymes: AsuHPI, TaqI
Cloning / PCR primers
|
73 | 83 | 68 | 17 | 56 | 74 |
0 - 0 - 1 - 16 - 121
0 - 0 - 0 - 1 - 2 138 off-targets |
show all... show less... |
329 / rev |
CCATCCCCGGACTCTCCAGA GGG
.................... ... Enzymes: LpnPI, Hpy188III, BslI
Cloning / PCR primers
|
72 | 86 | 50 | 36 | 73 | 80 |
0 - 0 - 1 - 21 - 103
0 - 0 - 0 - 3 - 2 125 off-targets |
show all... show less... |
349 / fw |
CCCTCTGGAGAGTCCGGGGA TGG
.................... ... Enzymes: NciI, BtsCI, BseDI, StyD4I
Cloning / PCR primers
|
72 | 83 | 46 | 64 | 63 | 57 |
0 - 0 - 4 - 11 - 317
0 - 0 - 1 - 0 - 2 332 off-targets |
show all... show less... |
328 / rev |
CATCCCCGGACTCTCCAGAG GGG
.................... ... Enzymes: LpnPI, Hpy188III, BslI
Cloning / PCR primers
|
71 | 85 | 62 | 61 | 75 | 83 |
0 - 0 - 1 - 20 - 108
0 - 0 - 1 - 2 - 6 129 off-targets |
show all... show less... |
407 / fw |
GGTCAGCTTGGCACTCTGGT TGG
.................... ... Cloning / PCR primers |
71 | 88 | 46 | 35 | 70 | 69 |
0 - 0 - 2 - 22 - 103
0 - 0 - 0 - 0 - 4 127 off-targets |
show all... show less... |
345 / fw |
CCCCCCCTCTGGAGAGTCCG GGG
.................... ... Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
Cloning / PCR primers
|
70 | 83 | 56 | 59 | 64 | 74 |
0 - 0 - 3 - 10 - 128
0 - 0 - 2 - 1 - 0 141 off-targets |
show all... show less... |
629 / fw |
GGAGCGGCCATGCTTGGCCC GGG
.................... ... Enzymes: BshFI, BmeT110I, NlaIV, BslFI, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
Cloning / PCR primers
|
70 | 87 | 43 | 52 | 64 | 70 |
0 - 0 - 2 - 10 - 123
0 - 0 - 0 - 4 - 4 135 off-targets |
show all... show less... |
343 / fw |
CTCCCCCCCTCTGGAGAGTC CGG
.................... ... Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
Cloning / PCR primers
|
69 | 82 | 32 | 59 | 59 | 70 |
0 - 0 - 4 - 24 - 152
0 - 0 - 0 - 2 - 4 180 off-targets |
show all... show less... |
384 / fw |
ACATGGTTATGTTTCTGGAT GGG
.................... ... Enzymes: Hpy188III, BtsCI
Cloning / PCR primers
|
69 | 80 | 42 | 25 | 65 | 77 |
0 - 0 - 4 - 18 - 179
0 - 0 - 2 - 2 - 3 201 off-targets |
show all... show less... |
669 / fw |
CGGACAGCCTCTCTGGCCAA GGG
.................... ... Enzymes: BshFI, ErhI, BalI, BseDI, AcoI
Cloning / PCR primers
|
69 | 86 | 58 | 83 | 63 | 81 |
0 - 0 - 2 - 15 - 102
0 - 0 - 1 - 1 - 5 119 off-targets |
show all... show less... |
141 / fw |
GCAATGGGGCTTCCTCCCAG AGG
.................... ... Enzymes: BstENI, LpnPI, BslI
Cloning / PCR primers
|
68 | 83 | 68 | 69 | 69 | 86 |
0 - 0 - 2 - 18 - 150
0 - 0 - 1 - 6 - 7 170 off-targets |
show all... show less... |
539 / rev |
CATCGACCGGCTCCTCCTCC TGG
.................... ... Enzymes: BshFI, MnlI, LpnPI, BstNI, BslI, StyD4I
Cloning / PCR primers
|
68 | 88 | 38 | 18 | 73 | 66 |
0 - 0 - 0 - 16 - 97
0 - 0 - 0 - 4 - 12 113 off-targets |
show all... show less... |
582 / fw |
GACTAGCAGGCAGTGCTGCA GGG
.................... ... Enzymes: PstI, BfmI, ApeKI, Fsp4HI, HpyCH4V
Cloning / PCR primers
|
68 | 88 | 54 | 45 | 64 | 89 |
0 - 0 - 3 - 21 - 126
0 - 0 - 0 - 4 - 4 150 off-targets |
show all... show less... |
206 / fw |
GCTCCCCACTGCCATCTCCG CGG
.................... ... Enzymes: BstDSI, MwoI, SacII, BspFNI, MspA1I, BseDI, Bsh1285I, SsiI
Cloning / PCR primers
|
67 | 81 | 70 | 37 | 51 | 64 |
0 - 0 - 1 - 17 - 193
0 - 0 - 0 - 0 - 2 211 off-targets |
show all... show less... |
326 / rev |
TCCCCGGACTCTCCAGAGGG GGG
.................... ... Enzymes: BslI
Cloning / PCR primers
|
67 | 72 | 51 | 67 | 72 | 79 |
0 - 0 - 1 - 26 - 161
0 - 0 - 0 - 3 - 8 188 off-targets |
show all... show less... |
379 / fw |
AAAGGACATGGTTATGTTTC TGG
.................... ... Inefficient Enzymes: Hpy188III, BtsCI
Cloning / PCR primers
|
67 | 79 | 21 | 36 | 55 | 67 |
0 - 0 - 3 - 32 - 234
0 - 0 - 0 - 2 - 2 269 off-targets |
show all... show less... |
656 / rev |
TAGGTTGCCCTTGGCCAGAG AGG
.................... ... Enzymes: LpnPI, MwoI, BglI
Cloning / PCR primers
|
67 | 79 | 58 | 88 | 72 | 84 |
0 - 0 - 2 - 17 - 130
0 - 0 - 1 - 2 - 14 149 off-targets |
show all... show less... |
144 / fw |
ATGGGGCTTCCTCCCAGAGG CGG
.................... ... Enzymes: BstENI, BslI
Cloning / PCR primers
|
66 | 79 | 63 | 83 | 64 | 81 |
0 - 0 - 7 - 24 - 132
0 - 0 - 3 - 6 - 8 163 off-targets |
show all... show less... |
330 / rev |
TCCATCCCCGGACTCTCCAG AGG
.................... ... Enzymes: LpnPI, Hpy188III, BslI
Cloning / PCR primers
|
66 | 82 | 51 | 60 | 73 | 74 |
0 - 0 - 3 - 23 - 132
0 - 0 - 0 - 1 - 2 158 off-targets |
show all... show less... |
321 / rev |
GGACTCTCCAGAGGGGGGGA GGG
.................... ... Enzymes: BslI
Cloning / PCR primers
|
65 | 66 | 46 | 54 | 65 | 74 |
0 - 0 - 4 - 22 - 231
0 - 0 - 0 - 0 - 3 257 off-targets |
show all... show less... |
344 / fw |
TCCCCCCCTCTGGAGAGTCC GGG
.................... ... Enzymes: PpsI, MspI, BtsCI, BseDI, NciI, LpnPI, StyD4I, HinfI
Cloning / PCR primers
|
65 | 79 | 43 | 40 | 59 | 65 |
0 - 0 - 1 - 22 - 154
0 - 0 - 0 - 6 - 8 177 off-targets |
show all... show less... |
383 / fw |
GACATGGTTATGTTTCTGGA TGG
.................... ... Enzymes: Hpy188III, BtsCI
Cloning / PCR primers
|
65 | 74 | 47 | 33 | 67 | 80 |
0 - 0 - 1 - 34 - 174
0 - 0 - 0 - 3 - 4 209 off-targets |
show all... show less... |
446 / rev |
TCTCTGGCGGTTCAGGCAGC GGG
.................... ... Enzymes: MspA1I, BstC8I, ApeKI, Fsp4HI, BstV1I
Cloning / PCR primers
|
65 | 85 | 41 | 63 | 73 | 68 |
0 - 0 - 0 - 15 - 169
0 - 0 - 0 - 1 - 1 184 off-targets |
show all... show less... |
367 / fw |
GATGGAGAGCCGAAAGGACA TGG
.................... ... Enzymes: NlaIII, SmiMI
Cloning / PCR primers
|
64 | 80 | 56 | 50 | 52 | 74 |
0 - 0 - 0 - 23 - 219
0 - 0 - 0 - 2 - 0 242 off-targets |
show all... show less... |
517 / rev |
GCCTCCCCCGCCACGCTCCC GGG
.................... ... High GC content Enzymes: BmeT110I, MspI, BseDI, NciI, LpnPI, StyD4I, LmnI, Cfr9I
Cloning / PCR primers
|
64 | 81 | 39 | 46 | 75 | 85 |
0 - 0 - 4 - 24 - 244
0 - 0 - 0 - 0 - 3 272 off-targets |
show all... show less... |
283 / fw |
CTCTTCTCAGCCAAGATCCC AGG
.................... ... Enzymes: NlaIV, NdeII, MflI, BseDI, LpnPI, BstNI, StyD4I, PasI
Cloning / PCR primers
|
63 | 76 | 43 | 31 | 66 | 81 |
0 - 0 - 3 - 17 - 285
0 - 0 - 0 - 1 - 2 305 off-targets |
show all... show less... |
322 / rev |
CGGACTCTCCAGAGGGGGGG AGG
.................... ... Enzymes: BslI
Cloning / PCR primers
|
63 | 73 | 30 | 78 | 67 | 73 |
0 - 0 - 1 - 10 - 175
0 - 0 - 0 - 0 - 5 186 off-targets |
show all... show less... |
342 / rev |
TCCTTTCGGCTCTCCATCCC CGG
.................... ... Enzymes: MspI, BseDI, NciI, LpnPI, StyD4I, HinfI, BccI
Cloning / PCR primers
|
63 | 82 | 45 | 40 | 64 | 66 |
0 - 0 - 0 - 27 - 182
0 - 0 - 0 - 4 - 6 209 off-targets |
show all... show less... |
386 / fw |
ATGGTTATGTTTCTGGATGG GGG
.................... ... Enzymes: BtsCI
Cloning / PCR primers
|
63 | 80 | 57 | 78 | 51 | 80 |
0 - 0 - 1 - 37 - 202
0 - 0 - 0 - 9 - 4 240 off-targets |
show all... show less... |
395 / fw |
TTTCTGGATGGGGGTCAGCT TGG
.................... ... Enzymes: AluBI
Cloning / PCR primers
|
63 | 81 | 43 | 43 | 64 | 80 |
0 - 0 - 9 - 50 - 159
0 - 0 - 2 - 5 - 5 218 off-targets |
show all... show less... |
325 / rev |
CCCCGGACTCTCCAGAGGGG GGG
.................... ... Enzymes: BslI
Cloning / PCR primers
|
62 | 81 | 57 | 61 | 70 | 79 |
1 - 0 - 3 - 16 - 107
1 - 0 - 1 - 2 - 4 127 off-targets |
show all... show less... |
281 / rev |
GCTCCTAATCCCAGGCTCCC TGG
.................... ... Enzymes: NlaIV, NdeII, BspPI, BseDI, MflI, LpnPI, BstNI, LmnI, StyD4I, PasI
Cloning / PCR primers
|
61 | 72 | 42 | 9 | 66 | 85 |
0 - 0 - 5 - 23 - 246
0 - 0 - 1 - 6 - 13 274 off-targets |
show all... show less... |
298 / fw |
ATCCCAGGGAGCCTGGGATT AGG
.................... ... Enzymes: AluBI
Cloning / PCR primers
|
61 | 78 | 39 | 54 | 62 | 71 |
0 - 0 - 2 - 34 - 222
0 - 0 - 0 - 4 - 8 258 off-targets |
show all... show less... |
668 / fw |
GCGGACAGCCTCTCTGGCCA AGG
.................... ... Enzymes: BshFI, BalI, MwoI, BseDI, BglI, ErhI, AcoI
Cloning / PCR primers
|
61 | 83 | 44 | 39 | 65 | 74 |
0 - 0 - 4 - 22 - 265
0 - 0 - 0 - 5 - 3 291 off-targets |
show all... show less... |
262 / rev |
CTGGGATCTTGGCTGAGAAG AGG
.................... ... Enzymes: AluBI, BseRI, MboII
Cloning / PCR primers
|
60 | 75 | 52 | 64 | 70 | 73 |
0 - 0 - 7 - 33 - 251
0 - 0 - 2 - 4 - 2 291 off-targets |
show all... show less... |
356 / rev |
GAAACATAACCATGTCCTTT CGG
.................... ... Inefficient Cloning / PCR primers |
60 | 82 | 39 | 20 | 52 | 74 |
0 - 0 - 6 - 35 - 221
0 - 0 - 1 - 3 - 7 262 off-targets |
show all... show less... |
472 / fw |
GCCTGAACCGCCAGAGAAGA TGG
.................... ... Enzymes: Bsp1286I, BanI, BaeGI, NlaIV, MboII
Cloning / PCR primers
|
60 | 82 | 46 | 45 | 65 | 63 |
0 - 0 - 1 - 30 - 148
0 - 0 - 0 - 3 - 0 179 off-targets |
show all... show less... |
327 / rev |
ATCCCCGGACTCTCCAGAGG GGG
.................... ... Enzymes: Hpy188III, BslI
Cloning / PCR primers
|
59 | 63 | 52 | 60 | 74 | 83 |
0 - 0 - 4 - 16 - 176
0 - 0 - 4 - 6 - 3 196 off-targets |
show all... show less... |
3 / rev |
TCTGCCACAGTGAAGATGAA AGG
.................... ... Enzymes: TspDTI
Cloning / PCR primers
|
57 | 75 | 57 | 32 | 72 | 82 |
0 - 1 - 5 - 40 - 282
0 - 0 - 1 - 4 - 8 328 off-targets |
show all... show less... |
126 / fw |
CTTAGAAAGAAGAGGGCAAT GGG
.................... ... Enzymes: BseMI
Cloning / PCR primers
|
57 | 77 | 43 | 52 | 66 | 76 |
0 - 0 - 6 - 38 - 270
0 - 0 - 0 - 7 - 2 314 off-targets |
show all... show less... |
280 / rev |
CTCCTAATCCCAGGCTCCCT GGG
.................... ... Enzymes: NlaIV, NdeII, BspPI, BseDI, MflI, LpnPI, BstNI, LmnI, StyD4I, PasI
Cloning / PCR primers
|
57 | 77 | 49 | 40 | 66 | 73 |
0 - 0 - 6 - 32 - 198
0 - 0 - 1 - 9 - 6 236 off-targets |
4:exon:A2m
show all...
show less...
|
581 / fw |
GGACTAGCAGGCAGTGCTGC AGG
.................... ... Enzymes: HpyCH4V, PstI, ApeKI, TspRI, BfmI, Fsp4HI
Cloning / PCR primers
|
56 | 80 | 38 | 15 | 64 | 48 |
0 - 0 - 4 - 32 - 196
0 - 0 - 0 - 2 - 14 232 off-targets |
show all... show less... |
292 / fw |
GCCAAGATCCCAGGGAGCCT GGG
.................... ... Inefficient Enzymes: StyD4I, LpnPI, NlaIV, BstNI, BseDI
Cloning / PCR primers
|
55 | 75 | 52 | 31 | 70 | 81 |
0 - 0 - 4 - 30 - 244
0 - 0 - 1 - 7 - 9 278 off-targets |
show all... show less... |
482 / fw |
CCAGAGAAGATGGTGCCCCA CGG
.................... ... Enzymes: Bsp1286I, BstDSI, BaeGI, HpyCH4III, BseDI
Cloning / PCR primers
|
55 | 79 | 69 | 64 | 67 | 90 |
0 - 1 - 17 - 30 - 184
0 - 0 - 2 - 3 - 1 232 off-targets |
show all... show less... |
273 / rev |
TCCCAGGCTCCCTGGGATCT TGG
.................... ... Enzymes: BspPI, BstDEI, MflI, NdeII
Cloning / PCR primers
|
54 | 72 | 34 | 43 | 64 | 81 |
0 - 0 - 4 - 46 - 325
0 - 0 - 1 - 4 - 16 375 off-targets |
show all... show less... |
626 / rev |
CGGAGGGGACTGGGGGTCCC GGG
.................... ... High GC content Enzymes: BshFI, BmeT110I, NlaIV, Eam1105I, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
Cloning / PCR primers
|
54 | 69 | 39 | 80 | 64 | 76 |
0 - 0 - 0 - 24 - 268
0 - 0 - 0 - 4 - 10 292 off-targets |
show all... show less... |
284 / fw |
TCTTCTCAGCCAAGATCCCA GGG
.................... ... Enzymes: NlaIV, MflI, BseDI, LpnPI, BstNI, StyD4I, PasI
Cloning / PCR primers
|
52 | 76 | 63 | 35 | 66 | 84 |
0 - 1 - 6 - 38 - 286
0 - 0 - 1 - 1 - 1 331 off-targets |
show all... show less... |
320 / rev |
GACTCTCCAGAGGGGGGGAG GGG
.................... ... Cloning / PCR primers |
52 | 63 | 37 | 65 | 68 | 82 |
0 - 0 - 5 - 29 - 313
0 - 0 - 0 - 3 - 8 347 off-targets |
show all... show less... |
587 / fw |
GCAGGCAGTGCTGCAGGGCT GGG
.................... ... Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI
Cloning / PCR primers
|
52 | 70 | 30 | 72 | 60 | 85 |
0 - 0 - 1 - 51 - 354
0 - 0 - 1 - 3 - 12 406 off-targets |
show all... show less... |
127 / fw |
TTAGAAAGAAGAGGGCAATG GGG
.................... ... Enzymes: BseMI
Cloning / PCR primers
|
51 | 53 | 73 | 42 | 68 | 92 |
0 - 0 - 4 - 64 - 490
0 - 0 - 0 - 4 - 7 558 off-targets |
More than 500 off-targets, showing 7 with score >1.0 show all... show less... |
291 / fw |
AGCCAAGATCCCAGGGAGCC TGG
.................... ... Inefficient Enzymes: StyD4I, LpnPI, NlaIV, BstNI, BseDI
Cloning / PCR primers
|
51 | 70 | 41 | 16 | 66 | 81 |
0 - 0 - 2 - 43 - 315
0 - 0 - 0 - 7 - 10 360 off-targets |
show all... show less... |
118 / fw |
GATGACAGCTTAGAAAGAAG AGG
.................... ... Enzymes: MboII
Cloning / PCR primers
|
50 | 68 | 61 | 30 | 72 | 71 |
0 - 0 - 6 - 33 - 297
0 - 0 - 1 - 5 - 13 336 off-targets |
show all... show less... |
254 / rev |
TTGGCTGAGAAGAGGAGCTA AGG
.................... ... Enzymes: AluBI, BstDEI
Cloning / PCR primers
|
50 | 71 | 55 | 56 | 71 | 84 |
0 - 0 - 5 - 49 - 301
0 - 0 - 1 - 9 - 5 355 off-targets |
show all... show less... |
544 / fw |
GCGTGGCGGGGGAGGCCAGG AGG
.................... ... High GC content Enzymes: StyD4I, BseRI, LpnPI, BstNI, BslI
Cloning / PCR primers
|
47 | 65 | 43 | 83 | 62 | 88 |
0 - 0 - 6 - 46 - 427
0 - 0 - 3 - 9 - 8 479 off-targets |
show all... show less... |
181 / rev |
GAGATGGCAGTGGGGAGCTA GGG
.................... ... Enzymes: AluBI, Tsp45I, BstEII, MaeIII, MaeI
Cloning / PCR primers
|
46 | 66 | 54 | 68 | 66 | 76 |
0 - 0 - 11 - 44 - 439
0 - 0 - 3 - 4 - 7 494 off-targets |
show all... show less... |
385 / fw |
CATGGTTATGTTTCTGGATG GGG
.................... ... Enzymes: Hpy188III, BtsCI
Cloning / PCR primers
|
46 | 71 | 37 | 54 | 58 | 76 |
0 - 0 - 3 - 30 - 369
0 - 0 - 0 - 1 - 5 402 off-targets |
show all... show less... |
541 / fw |
GGAGCGTGGCGGGGGAGGCC AGG
.................... ... Inefficient High GC content Enzymes: BshFI, MwoI, BseRI, LpnPI, BstNI, StyD4I
Cloning / PCR primers
|
46 | 73 | 37 | 59 | 73 | 80 |
0 - 0 - 2 - 44 - 468
0 - 0 - 0 - 5 - 9 514 off-targets |
More than 500 off-targets, showing 13 with score >1.0 show all... show less... |
125 / fw |
GCTTAGAAAGAAGAGGGCAA TGG
.................... ... Enzymes: BseMI
Cloning / PCR primers
|
45 | 61 | 55 | 32 | 66 | 78 |
0 - 0 - 5 - 50 - 442
0 - 0 - 0 - 6 - 18 497 off-targets |
show all... show less... |
586 / fw |
AGCAGGCAGTGCTGCAGGGC TGG
.................... ... Enzymes: NlaIV, AcyI, BfoI, HinP1I, BanI, SspDI
Cloning / PCR primers
|
45 | 68 | 35 | 37 | 62 | 82 |
0 - 1 - 9 - 52 - 431
0 - 0 - 1 - 4 - 6 493 off-targets |
show all... show less... |
319 / rev |
ACTCTCCAGAGGGGGGGAGG GGG
.................... ... Cloning / PCR primers |
38 | 49 | 42 | 75 | 67 | 82 |
0 - 0 - 6 - 60 - 542
0 - 0 - 0 - 6 - 7 608 off-targets |
More than 500 off-targets, showing 17 with score >1.0 show all... show less... |
119 / fw |
ATGACAGCTTAGAAAGAAGA GGG
.................... ... Enzymes: BseMI, MboII
Cloning / PCR primers
|
36 | 63 | 59 | 59 | 70 | 58 |
0 - 2 - 7 - 81 - 397
0 - 0 - 1 - 10 - 17 487 off-targets |
show all... show less... |
334 / fw |
GTTTTCCCCCTCCCCCCCTC TGG
.................... ... Not with U6/U3 Enzymes: BpmI, BslI, Hpy188III, MnlI
Cloning / PCR primers
|
36 | 59 | 36 | 44 | 68 | 72 |
0 - 1 - 7 - 67 - 471
0 - 0 - 2 - 3 - 12 546 off-targets |
More than 500 off-targets, showing 23 with score >1.0 show all... show less... |
627 / rev |
GCGGAGGGGACTGGGGGTCC CGG
.................... ... High GC content Enzymes: BshFI, BmeT110I, NlaIV, Eam1105I, MspI, PpuMI, EcoO109I, BseDI, PspPI, Bme18I, NciI, LpnPI, StyD4I, KflI, Cfr9I
Cloning / PCR primers
|
34 | 77 | 34 | 85 | 63 | 71 |
0 - 1 - 2 - 25 - 332
0 - 1 - 1 - 4 - 4 360 off-targets |
show all... show less... |
536 / fw |
TCCCGGGAGCGTGGCGGGGG AGG
.................... ... High GC content Enzymes: BshFI, StyD4I, LpnPI, MwoI, BstNI
Cloning / PCR primers
|
33 | 70 | 41 | 79 | 64 | 71 |
0 - 1 - 2 - 38 - 439
0 - 0 - 0 - 2 - 5 480 off-targets |
show all... show less... |
182 / rev |
GGAGATGGCAGTGGGGAGCT AGG
.................... ... Enzymes: AluBI, Tsp45I, MaeIII, MaeI
Cloning / PCR primers
|
26 | 51 | 39 | 57 | 66 | 79 |
0 - 3 - 12 - 78 - 964
0 - 0 - 3 - 7 - 13 1057 off-targets |
More than 500 off-targets, showing 23 with score >1.0 show all... show less... |
459 / rev |
TGGGGCACCATCTTCTCTGG CGG
.................... ... Cloning / PCR primers |
24 | 72 | 66 | 69 | 60 | 75 |
0 - 0 - 1241 - 21 - 138
0 - 0 - 1 - 3 - 1 1400 off-targets |
More than 500 off-targets, showing 24 with score >0.5 show all... show less... |
547 / fw |
TGGCGGGGGAGGCCAGGAGG AGG
.................... ... High GC content Enzymes: BseRI, NlaIV, BslI
Cloning / PCR primers
|
19 | 33 | 37 | 95 | 57 | 74 |
0 - 0 - 6 - 158 - 1190
0 - 0 - 1 - 18 - 30 1354 off-targets |
More than 500 off-targets, showing 12 with score >2.0 show all... show less... |
641 / rev |
CAGAGAGGCTGTCCGCGGAG GGG
.................... ... Enzymes: BstDSI, Bse1I, BslFI, SacII, MspA1I, BseDI, BslI
Cloning / PCR primers
|
16 | 32 | 59 | 83 | 70 | 72 |
0 - 0 - 0 - 1338 - 568
0 - 0 - 0 - 0 - 1 1906 off-targets |
More than 500 off-targets, showing 4 with score >0.5 show all... show less... |
553 / fw |
GGGAGGCCAGGAGGAGGAGC CGG
.................... ... Enzymes: NlaIV, MspI, TaqI, BseRI, LpnPI, Bsh1285I, Cfr10I
Cloning / PCR primers
|
5 | 15 | 48 | 71 | 66 | 56 |
0 - 2 - 35 - 378 - 4403
0 - 0 - 9 - 72 - 1098 4818 off-targets |
More than 500 off-targets, showing 20 with score >2.0 show all... show less... |