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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314009_F | TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314009_R | GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC | 60.2 | 1.00 |
| mm4_intergenic_Gulp1|Col3a1_chr1_44857113_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gulp1|Col3a1_chr1_44857113_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intron_Ankrd27_chr7_35607003_F | TCGTCGGCAGCGTCCAGGACACTTTCTGCCTGGA | 59.6 | 0.39 |
| mm4_intron_Ankrd27_chr7_35607003_R | GTCTCGTGGGCTCGGTGCAAAGAGAAACAGGCCCA | 60.1 | 0.39 |
| mm4_intergenic_Ptpre|Mir1962_chr7_135557517_F | TCGTCGGCAGCGTCAGGGAGAGCGTAGAGTGAGT | 59.3 | 0.36 |
| mm4_intergenic_Ptpre|Mir1962_chr7_135557517_R | GTCTCGTGGGCTCGGGGTTTTAGCTCACCACAGGC | 59.1 | 0.36 |
| mm4_intron_Csmd2_chr4_128030229_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Csmd2_chr4_128030229_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Crbn|Gm22418_chr6_106800125_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Crbn|Gm22418_chr6_106800125_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_BC034090_chr1_155226592_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_BC034090_chr1_155226592_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Zfhx3|Gm20686_chr8_108915681_F | TCGTCGGCAGCGTCTTTGGAGGGAGGAGCTGAGT | 60.1 | 0.22 |
| mm4_intergenic_Zfhx3|Gm20686_chr8_108915681_R | GTCTCGTGGGCTCGGTGCTAAGACTCAGGTTGGCC | 59.6 | 0.22 |
| mm4_intron_Sipa1l1_chr12_82312091_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Sipa1l1_chr12_82312091_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Klhl29_chr12_5325959_F | TCGTCGGCAGCGTCACGCAAACTTCATGCACACC | 59.9 | 0.19 |
| mm4_intron_Klhl29_chr12_5325959_R | GTCTCGTGGGCTCGGTCCATTTGCCCAGTTGCTCT | 59.8 | 0.19 |
| mm4_intergenic_Nlrp2|Gm5065_chr7_5338828_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Nlrp2|Gm5065_chr7_5338828_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Mpp5|Gm25225_chr12_78749154_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Mpp5|Gm25225_chr12_78749154_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Hs6st2_chrX_51398282_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Hs6st2_chrX_51398282_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm16065|Tbx5_chr5_119749829_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm16065|Tbx5_chr5_119749829_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Prkce_chr17_86253445_F | TCGTCGGCAGCGTCGGTGCTCCCTTCCTTCTGC | 60.3 | 0.14 |
| mm4_intron_Prkce_chr17_86253445_R | GTCTCGTGGGCTCGGGCAATGAGCCAGAAGGGACT | 60.0 | 0.14 |
| mm4_intergenic_Gm15401|Tcf7l1_chr6_72643369_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm15401|Tcf7l1_chr6_72643369_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Mir1193|Mir666_chr12_109716456_F | TCGTCGGCAGCGTCGGGTGAAGGTGGCTTCCTTG | 60.8 | 0.13 |
| mm4_intergenic_Mir1193|Mir666_chr12_109716456_R | GTCTCGTGGGCTCGGTATGTCTCTGCCATGGCTGC | 60.1 | 0.13 |
| mm3_intergenic_St14|Aplp2_chr9_31131902_F | TCGTCGGCAGCGTCTAGTTTCAGGGTGTGGCCTC | 59.3 | 0.13 |
| mm3_intergenic_St14|Aplp2_chr9_31131902_R | GTCTCGTGGGCTCGGTCCTTTTCGGGGTTCTGGC | 59.9 | 0.13 |
| mm4_intergenic_Gabre|Magea10_chrX_72367459_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gabre|Magea10_chrX_72367459_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm15148|Phf8_chrX_151512564_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm15148|Phf8_chrX_151512564_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_4933402N22Rik|Sema3d_chr5_12240204_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_4933402N22Rik|Sema3d_chr5_12240204_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Ogfrl1|Gm7784_chr1_23383335_F | TCGTCGGCAGCGTCCACCAGCTCCACCGGGAC | 62.3 | 0.08 |
| mm4_intergenic_Ogfrl1|Gm7784_chr1_23383335_R | GTCTCGTGGGCTCGGGGATCAGGCTTCTCGCTTGG | 60.8 | 0.08 |
| mm4_intron_Zfp655_chr5_145232151_F | TCGTCGGCAGCGTCAAGAGAGAGGTAGGCGGAGG | 60.1 | 0.08 |
| mm4_intron_Zfp655_chr5_145232151_R | GTCTCGTGGGCTCGGGCCTGGTGTCCTTCAGATCC | 60.1 | 0.08 |
| mm4_intergenic_Gm25715|N6amt1_chr16_87001451_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25715|N6amt1_chr16_87001451_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Yipf1_chr4_107335190_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Yipf1_chr4_107335190_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tmem132d_chr5_128317049_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Tmem132d_chr5_128317049_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Gm3898_chr9_43832324_F | TCGTCGGCAGCGTCAGGGTGACTATGTGGGGAGC | 60.9 | 0.06 |
| mm4_intron_Gm3898_chr9_43832324_R | GTCTCGTGGGCTCGGAGCTCTAGGTGGAGACCCTG | 60.0 | 0.06 |
| mm4_intron_Zfp36l2_chr17_84187410_F | TCGTCGGCAGCGTCCGGGTCTAGCCATGTGATCC | 59.9 | 0.06 |
| mm4_intron_Zfp36l2_chr17_84187410_R | GTCTCGTGGGCTCGGGCGTGGAGAGAGCGAGTAG | 59.6 | 0.06 |
| mm4_intron_Batf_chr12_85698375_F | TCGTCGGCAGCGTCGCGTGGAGAGAGCGAGTAG | 59.6 | 0.06 |
| mm4_intron_Batf_chr12_85698375_R | GTCTCGTGGGCTCGGCGGGTCTAGCCATGTGATCC | 59.9 | 0.06 |
| mm4_intergenic_Ighv1-26|Ighv1-30_chr12_114802620_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Ighv1-26|Ighv1-30_chr12_114802620_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Tmem132d_chr5_128375396_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Tmem132d_chr5_128375396_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Dnah9_chr11_65909837_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Dnah9_chr11_65909837_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_AW554918_chr18_25455138_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_AW554918_chr18_25455138_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm25062|Gm15487_chr6_37672225_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm25062|Gm15487_chr6_37672225_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm14823|Gm14855_chrX_105659280_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm14823|Gm14855_chrX_105659280_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Eif1a|Cdo1_chr18_46661249_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Eif1a|Cdo1_chr18_46661249_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Fank1_chr7_133853426_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Fank1_chr7_133853426_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tjp1_chr7_65370535_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tjp1_chr7_65370535_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Wnt2|Asz1_chr6_18031521_F | TCGTCGGCAGCGTCAGCGACCTTGAACTGTTCCA | 59.5 | 0.05 |
| mm4_intergenic_Wnt2|Asz1_chr6_18031521_R | GTCTCGTGGGCTCGGGCGTGCCTGCTCGATATACA | 60.3 | 0.05 |
| mm4_intergenic_Gm25787|Gm23345_chr7_111835187_F | TCGTCGGCAGCGTCCTTCTGAGGAGCGGGTGTAC | 59.8 | 0.04 |
| mm4_intergenic_Gm25787|Gm23345_chr7_111835187_R | GTCTCGTGGGCTCGGGGAGGGAATGGCACTTTGGA | 59.9 | 0.04 |
| mm4_intron_Tcf4_chr18_69376616_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Tcf4_chr18_69376616_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Slc4a1|Bloodlinc_chr11_102373805_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Slc4a1|Bloodlinc_chr11_102373805_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prune2_chr19_16996743_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prune2_chr19_16996743_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Pcolce_chr5_137610686_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Pcolce_chr5_137610686_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pard3|mmu-mir-21c_chr8_127792312_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Pard3|mmu-mir-21c_chr8_127792312_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rapgef5|Cdca7l_chr12_117761379_F | TCGTCGGCAGCGTCCCCTCCACTGCCTTCCAAAG | 60.6 | 0.02 |
| mm4_intergenic_Rapgef5|Cdca7l_chr12_117761379_R | GTCTCGTGGGCTCGGGGTGTGGTTAGGCATCAGGG | 60.3 | 0.02 |
| mm4_intergenic_Elk3|mmu-mir-7688_chr10_93408611_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Elk3|mmu-mir-7688_chr10_93408611_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gas7_chr11_67672252_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gas7_chr11_67672252_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Nudt4_chr10_95563810_F | TCGTCGGCAGCGTCCGTCTGGTTGGGCTTGAACT | 60.5 | 0.02 |
| mm4_exon_Nudt4_chr10_95563810_R | GTCTCGTGGGCTCGGGAGCATCGCGACTTCCTTCG | 61.4 | 0.02 |
| mm4_intron_Nkx6-3_chr8_23154498_F | TCGTCGGCAGCGTCGCAGAACCAGAGCGCTAAGA | 60.1 | 0.02 |
| mm4_intron_Nkx6-3_chr8_23154498_R | GTCTCGTGGGCTCGGCCCCTCCACTCCAGTTCTCA | 60.5 | 0.02 |
| mm4_intergenic_Gm1968|4632428C04Rik_chr16_29974034_F | TCGTCGGCAGCGTCTCCTCAACCCTCCATGACCA | 60.1 | 0.02 |
| mm4_intergenic_Gm1968|4632428C04Rik_chr16_29974034_R | GTCTCGTGGGCTCGGGTCACTCACTCCAGCGTCTC | 60.1 | 0.02 |
| mm4_intergenic_Top2a|Igfbp4_chr11_99039390_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Top2a|Igfbp4_chr11_99039390_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_A830012C17Rik|Gm11780_chr4_4252354_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_A830012C17Rik|Gm11780_chr4_4252354_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cacna1h_chr17_25408613_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cacna1h_chr17_25408613_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_2900056M20Rik|Tspyl2_chrX_152330315_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_2900056M20Rik|Tspyl2_chrX_152330315_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Camk2g|Plau_chr14_20794152_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Camk2g|Plau_chr14_20794152_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Zeb2/Gm13476/ZEB2_AS1_3/ZEB2_AS1_4|Zeb2_chr2_45117172_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Zeb2/Gm13476/ZEB2_AS1_3/ZEB2_AS1_4|Zeb2_chr2_45117172_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Olfm1_chr2_28193659_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Olfm1_chr2_28193659_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cd200_chr16_45407353_F | TCGTCGGCAGCGTCCGGTAACCAGGGAACACTCC | 60.0 | 0.01 |
| mm4_intron_Cd200_chr16_45407353_R | GTCTCGTGGGCTCGGACCCTGAGATTAGTACTGATGTCT | 58.2 | 0.01 |
| mm4_intron_Fto_chr8_91369363_F | TCGTCGGCAGCGTCGGTGACCTGCTGAAGTACCC | 60.0 | 0.01 |
| mm4_intron_Fto_chr8_91369363_R | GTCTCGTGGGCTCGGCAGCTCACTTCAGGGCAGAA | 59.9 | 0.01 |
| mm4_intergenic_Ano2|Ntf3_chr6_126048190_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ano2|Ntf3_chr6_126048190_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fer1l5_chr1_36377127_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fer1l5_chr1_36377127_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vmn2r47|Vmn2r-ps51_chr7_9877900_F | TCGTCGGCAGCGTCAAGGAAAGACTCGAGCAGCA | 59.3 | 0.00 |
| mm4_intergenic_Vmn2r47|Vmn2r-ps51_chr7_9877900_R | GTCTCGTGGGCTCGGTGGACAGTGGAGAAGCAGTG | 59.6 | 0.00 |
| mm4_intergenic_Gm3912|Vmn2r31_chr7_7375567_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm3912|Vmn2r31_chr7_7375567_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm20482|Vmn2r-ps43_chr7_8863787_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm20482|Vmn2r-ps43_chr7_8863787_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2810047C21Rik1|Gm3665_chr7_8093973_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2810047C21Rik1|Gm3665_chr7_8093973_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bub3|Gm16477_chr7_131587354_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bub3|Gm16477_chr7_131587354_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bub1b|Pak6_chr2_118650766_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bub1b|Pak6_chr2_118650766_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17828757_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17828757_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ino80_chr2_119417688_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ino80_chr2_119417688_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55644347_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55644347_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tec_chr5_72846723_F | TCGTCGGCAGCGTCTCCAATGATTCTGCTCCGCC | 60.4 | 0.00 |
| mm4_intron_Tec_chr5_72846723_R | GTCTCGTGGGCTCGGACCATTTTATGAGGCACATGGC | 59.5 | 0.00 |
| mm4_intron_Gm15128_chrX_147801145_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm15128_chrX_147801145_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm15127_chrX_148675131_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm15127_chrX_148675131_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mirlet7i|Mon2_chr10_122985942_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mirlet7i|Mon2_chr10_122985942_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314009 |
AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA GGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG |
| mm4_intron_Ankrd27_chr7_35607003 |
CAGGACACTTTCTGCCTGGAGTGTGGTTTCCCGGGGGCGTGGCTGTGGTCTTGGTTGGTGGAGCAGGGCTCCGTCCTCCC GCTTGGGGCTCATGGAGGCTGTGGGCCTGTTTCTCTTTGCA |
| mm4_intergenic_Ptpre|Mir1962_chr7_135557517 |
AGGGAGAGCGTAGAGTGAGTCACTTCCTGAATCGATTGAACTTTCAGAAGAAAAGCAATCTCTCTTTCCCCGGAGCATGG CGGAGGACTCTTTATTATTGGTTACAGTTGCCTGTGGTGAGCTAAAACC |
| mm4_intergenic_Zfhx3|Gm20686_chr8_108915681 |
TTTGGAGGGAGGAGCTGAGTCATTTCTGAGAGGATTTACTGGGCAGATAAGTCTCACAGACACCACCACCACGCTAACAG GAAGTGCTGTGACAAGCAACAGACAGTATTGTTAGGCCAACCTGAGTCTTAGCA |
| mm4_intron_Klhl29_chr12_5325959 |
ACGCAAACTTCATGCACACCCTCATTCTGGGCAGTCTTTAAGCACAACGTCCAACTTCCAGGGACTGTGGCAGTGGAGGA CATGTCAGATGAAGAGAGCAACTGGGCAAATGGA |
| mm4_intron_Prkce_chr17_86253445 |
GGTGCTCCCTTCCTTCTGCCCACTGCCACTCCCCCAGGAAGTCTCTTCAAGTCTTTCTCATGCTGGTCTTTGTATTCTTC ATCCTTTCCAACTCCCAGTCCCTTCTGGCTCATTGC |
| mm4_intergenic_Mir1193|Mir666_chr12_109716456 | GGGTGAAGGTGGCTTCCTTGCCCCTCCTCCAGGCTCCTGGCAAGTGGTTCTTGCAGCCATGGCAGAGACATA |
| mm3_intergenic_St14|Aplp2_chr9_31131902 |
TAGTTTCAGGGTGTGGCCTCCAGGGTCGCCCCGCCCCTCCAGCCAGCCAGCACCGGCGCCCGCACACCTGTTGCGGCCGG TCCCGCCTTCCGCCACGCCCCCAGGAAGCTCTTTCCCTCCCGCCAGAACCCCGAAAAGGA |
| mm4_intergenic_Ogfrl1|Gm7784_chr1_23383335 |
CACCAGCTCCACCGGGACGCGCCCCCCTCCACGGTCCCCGGGAGTGGCCAGGGGTCAGCTGCCCCGCCCCCTGTGGACCG GGATCCGCCCCCTTGGGGCTCCTCCCAGCCAAGCGAGAAGCCTGATCC |
| mm4_intron_Zfp655_chr5_145232151 |
AAGAGAGAGGTAGGCGGAGGGTGGCTCTGGGCGAGGCCTGCGGACGGGCCGGGAGCCTGGCGGAGGGCGGTGGACGGTGG ACGGTGGACGGAACCCGGGCTCGTCCTTGGATCTGAAGGACACCAGGC |
| mm4_intron_Gm3898_chr9_43832324 |
AGGGTGACTATGTGGGGAGCACCCCCACCAAGCTCCAGGGCAGTCATCTGGAGCAGGGCGTGGGGTGGTCTGTACAGCTC CCTCTTTCTTTTCCAGGGTCTCCACCTAGAGCT |
| mm4_intron_Zfp36l2_chr17_84187410 |
CGGGTCTAGCCATGTGATCCTGGGCCCGTGGCCACGGGGTGGGCGGCGCAAACGCTGTCCGGGAAGTCCCGGCAGCGTGG CCGCGGTCGGTTCGGGAGGAGCACGGCTGCTCCAGCCGCTACTCGCTCTCTCCACGC |
| mm4_intron_Batf_chr12_85698375 |
GCGTGGAGAGAGCGAGTAGCGGCTGGAGCAGCCGTGCTCCTCCCGAACCGACCGCGGCCACGCTGCCGGGACTTCCCGGA CAGCGTTTGCGCCGCCCACCCCGTGGCCACGGGCCCAGGATCACATGGCTAGACCCG |
| mm4_intergenic_Wnt2|Asz1_chr6_18031521 |
AGCGACCTTGAACTGTTCCATTGCTCTTTTCCTGGGGCGGGGGCCCCACCGCCACCACCACTACCCAGGAGCGTGCCCGG GGGAATGGGGAGTATGAAGGGGTTGTATGGTCATTGTATATCGAGCAGGCACGC |
| mm4_intergenic_Gm25787|Gm23345_chr7_111835187 |
CTTCTGAGGAGCGGGTGTACTCCCCAGGCAAGCTCCTGGGAAGTAGATTTTGGACCAAGGTAAGGAGGGAAACCTCCGAG ATGAGTAAAAGAAAATGGATTTGGGGGGAAGTACCTGTTCCAAAGTGCCATTCCCTCC |
| mm4_intergenic_Rapgef5|Cdca7l_chr12_117761379 | CCCTCCACTGCCTTCCAAAGCCCCCCCCCCCCCCCACTCTCCAGGGAAGGGAATCTCCCTGATGCCTAACCACACC |
| mm4_exon_Nudt4_chr10_95563810 |
CGTCTGGTTGGGCTTGAACTTCATCCTAGAGCCGGGCTCCTGCAGCCGCTGCTCCGGCCGCCACCGTGGGGACCCGGCCG CCGCCACCCCGCCGGGGAGTCGGGCCGAAGGAAGTCGCGATGCTC |
| mm4_intron_Nkx6-3_chr8_23154498 |
GCAGAACCAGAGCGCTAAGATGACCTTGGCCCTGACTGCTAGGGANNNNNNNNNNNNNNNNNNNNNNTTACCCCACCCAT CCCTGAGACTCAGGTTTCTGAGAACTGGAGTGGAGGGG |
| mm4_intergenic_Gm1968|4632428C04Rik_chr16_29974034 |
TCCTCAACCCTCCATGACCAATTCTTCCTAAGCTATGGACGACTTGCCAGGAGCGGGGCAGTGGGCGCCACAGCTGGCTC CTCAAGGAGCTGCCTTTTGAGACGCTGGAGTGAGTGAC |
| mm4_intron_Cd200_chr16_45407353 |
CGGTAACCAGGGAACACTCCAGTCTAGAATGAATCCAAAATGTATTAGGTAAGACACATTATTTAACCTCCCTGGAGCCT GNNNNNNNNNNNNNNNNNNNNNNNNGGAAGACATCAGTACTAATCTCAGGGT |
| mm4_intron_Fto_chr8_91369363 |
GGTGACCTGCTGAAGTACCCAGTGCTTGGATTTCTGAGCCCTGAGCTACCCGGGAGCGGGGAGGGGGCAGACCTAAGGGT TGGGTGCTGCTCTTACTTCTGCCCTGAAGTGAGCTG |
| mm4_intergenic_Vmn2r47|Vmn2r-ps51_chr7_9877900 |
AAGGAAAGACTCGAGCAGCAGCTTNNNNNNNNNNNNNNNNNNNNNAAGGTAACACTAGAGTATATGTAACCGTATTTAGT CTGCAGGGAGTCCTGTTAACTCCTTAACACTGCTTCTCCACTGTCCA |
| mm4_intron_Tec_chr5_72846723 |
TCCAATGATTCTGCTCCGCCCTGAGAGTCAGAACTCAGAACTTCAATTGTTGAAACTGGAACTTCCCNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNAAGATAGCCATGTGCCTCATAAAATGGT |