CRISPOR

DNA break repair outcome predictions

Guide: CCAGAGAGGCTGTCCGCGGA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCA`	(Wild-type sequence)
389	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGGA-----CTGGGGGTCCCGGGCCAAGCATGGC`	`5 bp deleted → frameshift`
344	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG---GGACTGGGGGTCCCGGGCCAAGCATGGC`	`3 bp deleted → no frameshift`
327	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG----GACTGGGGGTCCCGGGCCAAGCATGGC`	`4 bp deleted → frameshift`
257	`TTGCCCTTGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGC`	`20 bp deleted → frameshift`
242	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG----------GGGTCCCGGGCCAAGCATGGC`	`10 bp deleted → frameshift`
232	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGC`	`19 bp deleted → frameshift`
230	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGGC`	`11 bp deleted → frameshift`
224	`TTGCCCTTGGCCAGAGAGGCTG----------------GGGGTCCCGGGCCAAGCATGGC`	`16 bp deleted → frameshift`
219	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGC`	`12 bp deleted → no frameshift`
210	`TTGCCCTTGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGC`	`21 bp deleted → no frameshift`
208	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGC`	`13 bp deleted → frameshift`
208	`TTGCCCTTGGCCAGAGAGG-------------GGACTGGGGGTCCCGGGCCAAGCATGGC`	`13 bp deleted → frameshift`
198	`TTGCCCTTGGCCAGAGAGG--------------GACTGGGGGTCCCGGGCCAAGCATGGC`	`14 bp deleted → frameshift`
188	`TTGCCCTTGGCCAGAGAGG---------------ACTGGGGGTCCCGGGCCAAGCATGGC`	`15 bp deleted → no frameshift`
147	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGC`	`20 bp deleted → frameshift`
147	`TTGCCCTTGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCATGGC`	`20 bp deleted → frameshift`
140	`TTGCCCTTGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGGC`	`21 bp deleted → no frameshift`
133	`TTGCCCTTGGCCAGAGAGGC------------------------------CAAGCATGGC`	`30 bp deleted → no frameshift`
133	`TTGCCCTTGGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGC`	`22 bp deleted → frameshift`
133	`TTGCCCTTGG----------------------GGACTGGGGGTCCCGGGCCAAGCATGGC`	`22 bp deleted → frameshift`
127	`TTGCCCTTGGCCA---------------------------------------AGCATGGC`	`39 bp deleted → no frameshift`
126	`TTGCCCTTGGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGC`	`23 bp deleted → frameshift`
126	`TTGCCCTTGGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGC`	`23 bp deleted → frameshift`
126	`TTGCCCTTGG-----------------------GACTGGGGGTCCCGGGCCAAGCATGGC`	`23 bp deleted → frameshift`
122	`TTGCCCTTGGCCAGAGA------------------CTGGGGGTCCCGGGCCAAGCATGGC`	`18 bp deleted → no frameshift`
120	`TTGCCCTTGG------------------------ACTGGGGGTCCCGGGCCAAGCATGGC`	`24 bp deleted → no frameshift`
117	`TTGCCCTTGG-----------------------------GGGTCCCGGGCCAAGCATGGC`	`29 bp deleted → frameshift`
110	`TTGCCCTTGGCCAGA--------------------CTGGGGGTCCCGGGCCAAGCATGGC`	`20 bp deleted → frameshift`
109	`TTGCCCTTGGCCAGAGAGGCTGTCC--------------------------AAGCATGGC`	`26 bp deleted → frameshift`
98	`TTGCCCTTGGCCAGAGAGGCTGTCCGC----------------------------ATGGC`	`28 bp deleted → frameshift`
94	`TTGCCCTTGGCCAGAGAGG-----------------------------GCCAAGCATGGC`	`29 bp deleted → frameshift`
89	`TTGCCCTTGGCCAGAGAGGCTGTCCGCGG------------------------------C`	`30 bp deleted → no frameshift`
89	`TTGCCCTTGG------------------------------GGTCCCGGGCCAAGCATGGC`	`30 bp deleted → no frameshift`
84	`TTGCCCTTGG-------------------------------GTCCCGGGCCAAGCATGGC`	`31 bp deleted → frameshift`
81	`TTGCCCTTGGCCAGAGAGGC----------------------------------------`	`40 bp deleted → frameshift`
81	`TTGCCC----------------------------------------GGGCCAAGCATGGC`	`40 bp deleted → frameshift`
80	`TTGCCCTTGG--------------------------------TCCCGGGCCAAGCATGGC`	`32 bp deleted → frameshift`
76	`TTGCCCTTGGCCAGAGAGGCTGTCCGC---------------------------------`	`33 bp deleted → no frameshift`
76	`TTGCCCTTGGCC---------------------------------CGGGCCAAGCATGGC`	`33 bp deleted → no frameshift`
73	`TTGCCCTTGGCC----------------------------------GGGCCAAGCATGGC`	`34 bp deleted → frameshift`
69	`TTGCCCTTGGCCAGAGAGGC-----------------------------------ATGGC`	`35 bp deleted → frameshift`
66	`TTGCCCT------------------------------GGGGGTCCCGGGCCAAGCATGGC`	`30 bp deleted → no frameshift`
60	`TTGCCCTTGGC-------------------------------------------------`	`49 bp deleted → frameshift`
60	`TTGCCCTTGG--------------------------------------GCCAAGCATGGC`	`38 bp deleted → frameshift`
60	`TTGCC----------------------------------------------AAGCATGGC`	`46 bp deleted → frameshift`
56	`TTGCCC---------------------------------------CGGGCCAAGCATGGC`	`39 bp deleted → no frameshift`
52	`TTG-----------------------------------GGGGTCCCGGGCCAAGCATGGC`	`35 bp deleted → frameshift`
51	`TTGCC-----------------------------------------GGGCCAAGCATGGC`	`41 bp deleted → frameshift`
49	`TTGCCCTTGGCCAGAGAGGCTG------------------------------------GC`	`36 bp deleted → no frameshift`
49	`TTGCCCTTGGCCAGAGAG------------------------------------CATGGC`	`36 bp deleted → no frameshift`
45	`TTGCCCTTGGCCAGAG--------------------------------------CATGGC`	`38 bp deleted → frameshift`
44	`TTGCCCTTGGC--------------------------------------------ATGGC`	`44 bp deleted → frameshift`
42	`TTGCCC---------------------------------------------AAGCATGGC`	`45 bp deleted → no frameshift`
40	`TTGCCCTTGGCCAG----------------------------------------CATGGC`	`40 bp deleted → frameshift`
34	`TTGCCCTTGGCCA-------------------------------------------TGGC`	`43 bp deleted → frameshift`
31	`TTGC---------------------------------------------------ATGGC`	`51 bp deleted → no frameshift`
24	`TTGC--------------------------------------------------------`	`56 bp deleted → frameshift`
19	`TTG-------------------------------------------------------GC`	`55 bp deleted → frameshift`

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