CRISPOR

DNA break repair outcome predictions

Guide: TCGCCGTCGACACGGCGCTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTGCCTTGTCGCCTTAGCTCCTCTTC`	(Wild-type sequence)
538	`ATCTCCGCGGTCGCCGTCGACACGGCGC----------------CTGCCTTGTCGCCTTA`	`16 bp deleted → frameshift`
402	`ATCTCCGCGGTCGCCGTCGACACGGC--------TACCCGGCGCCTGCCTTGTCGCCTTA`	`8 bp deleted → frameshift`
370	`ATCTCCGCGGTCGCCGTCGACACGGCGCT------ACCCGGCGCCTGCCTTGTCGCCTTA`	`6 bp deleted → no frameshift`
282	`ATCTCCGCGGTCGCCGTCGACACGG-------GCTACCCGGCGCCTGCCTTGTCGCCTTA`	`7 bp deleted → frameshift`
213	`ATCTCCGCGGTCGCCGTCGACACGGCGCTG-----------------CCTTGTCGCCTTA`	`17 bp deleted → frameshift`
208	`ATCTCCGCGGTCGCCGTCGACACGGCG-------------GCGCCTGCCTTGTCGCCTTA`	`13 bp deleted → frameshift`
198	`ATCTCCGCGGTCGCCGTCGACACGGCGC--------------GCCTGCCTTGTCGCCTTA`	`14 bp deleted → frameshift`
180	`ATCTCCGCGGTCGCCG------------------------GCGCCTGCCTTGTCGCCTTA`	`24 bp deleted → no frameshift`
178	`ATCTCCGCGGTCGCC------------------------------TGCCTTGTCGCCTTA`	`30 bp deleted → no frameshift`
162	`ATCTCCGCGGTCGCCGTCGACACGGC------------------CTGCCTTGTCGCCTTA`	`18 bp deleted → no frameshift`
154	`ATCTCCGCGGTCGCCGTCGACACG-------------------CCTGCCTTGTCGCCTTA`	`19 bp deleted → frameshift`
149	`ATCTCCGCGGTCGCCGTCGACAC--------------CCGGCGCCTGCCTTGTCGCCTTA`	`14 bp deleted → frameshift`
148	`ATCTCCGCGGTCGCCGTCGACACGGCGC----------------------------CTTA`	`28 bp deleted → frameshift`
147	`ATCTCCGCGGTCGCCGTCGACACGGCGC--------------------CTTGTCGCCTTA`	`20 bp deleted → frameshift`
147	`ATCTCCGCGGTCGC--------------------TACCCGGCGCCTGCCTTGTCGCCTTA`	`20 bp deleted → frameshift`
140	`ATCTCCGCGGTCGCCGTCG---------------------GCGCCTGCCTTGTCGCCTTA`	`21 bp deleted → no frameshift`
134	`ATCTCCGCGGTCGCCGTCGAC----------------CCGGCGCCTGCCTTGTCGCCTTA`	`16 bp deleted → frameshift`
134	`ATCTCCGCGGTCGCC------------------------------------------TTA`	`42 bp deleted → no frameshift`
133	`ATCTCCGCGGTCGCCGTCGACACGGC----------------------CTTGTCGCCTTA`	`22 bp deleted → frameshift`
133	`ATCTCCGCGG----------------------GCTACCCGGCGCCTGCCTTGTCGCCTTA`	`22 bp deleted → frameshift`
127	`ATCTCCGCGG-------------------------------CGCCTGCCTTGTCGCCTTA`	`31 bp deleted → frameshift`
126	`ATCTCCGCGGTCGCC-----------------------CGGCGCCTGCCTTGTCGCCTTA`	`23 bp deleted → frameshift`
126	`ATCTCCGCGG-----------------------CTACCCGGCGCCTGCCTTGTCGCCTTA`	`23 bp deleted → frameshift`
120	`ATCTCCGCGGTCGCCGTCG------------------------CCTGCCTTGTCGCCTTA`	`24 bp deleted → no frameshift`
115	`ATCTCCGCGGTCGCCGTCG------------------------------------CCTTA`	`36 bp deleted → no frameshift`
115	`ATCTCCG---------------------------------GCGCCTGCCTTGTCGCCTTA`	`33 bp deleted → no frameshift`
109	`ATCTCCGCGGTCGCC----------------------------------TTGTCGCCTTA`	`34 bp deleted → frameshift`
109	`ATCTCCGCG----------------------------------CCTGCCTTGTCGCCTTA`	`34 bp deleted → frameshift`
109	`ATCTCCGC--------------------------TACCCGGCGCCTGCCTTGTCGCCTTA`	`26 bp deleted → frameshift`
105	`ATCTCCGCGGTCGCCGTCGACACGGCGCT---------------------TGTCGCCTTA`	`21 bp deleted → no frameshift`
103	`ATCTCCGCGGTCGCCG---------------------------CCTGCCTTGTCGCCTTA`	`27 bp deleted → no frameshift`
103	`ATCTCCGCGGTCG---------------------------GCGCCTGCCTTGTCGCCTTA`	`27 bp deleted → no frameshift`
99	`ATCTCCGCGGTCGCCGTCGACACGGCGCTG----------------------TCGCCTTA`	`22 bp deleted → frameshift`
99	`ATCTCCGC------------------------------------CTGCCTTGTCGCCTTA`	`36 bp deleted → no frameshift`
98	`ATCTCCGCGGTCGC----------------------------GCCTGCCTTGTCGCCTTA`	`28 bp deleted → frameshift`
89	`ATCTCCGCGGTCGCCGTCGACACGGC------------------------------CTTA`	`30 bp deleted → no frameshift`
84	`ATCTCCGCGGTCGCCGTCGACACG-------------------------------CCTTA`	`31 bp deleted → frameshift`
80	`ATCTCC--------------------------------CGGCGCCTGCCTTGTCGCCTTA`	`32 bp deleted → frameshift`
70	`ATCTCCGCGGTCGCCGTCGACACGGCGCT-----------------------------TA`	`29 bp deleted → frameshift`
63	`ATCT-------------------------------ACCCGGCGCCTGCCTTGTCGCCTTA`	`31 bp deleted → frameshift`
56	`ATCTCCGCGGTCGCCG---------------------------------------CCTTA`	`39 bp deleted → no frameshift`
56	`ATCTCC---------------------------------------TGCCTTGTCGCCTTA`	`39 bp deleted → no frameshift`
54	`ATCTCCGC------------------------------------------------CTTA`	`48 bp deleted → no frameshift`
54	`ATCTCCGC----------------------------------------CTTGTCGCCTTA`	`40 bp deleted → frameshift`
46	`ATCTCC-------------------------------------------TTGTCGCCTTA`	`43 bp deleted → frameshift`
40	`ATCTCCGCG----------------------------------------------CCTTA`	`46 bp deleted → frameshift`
36	`ATCT------------------------------------------GCCTTGTCGCCTTA`	`42 bp deleted → no frameshift`
31	`ATCTCC---------------------------------------------------TTA`	`51 bp deleted → no frameshift`
30	`ATCT----------------------------------------------TGTCGCCTTA`	`46 bp deleted → frameshift`
25	`ATCTC-------------------------------------------------GCCTTA`	`49 bp deleted → frameshift`
23	`ATC---------------------------------------------------GCCTTA`	`51 bp deleted → no frameshift`
20	`ATCT------------------------------------------------------TA`	`54 bp deleted → no frameshift`

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