CRISPOR

DNA break repair outcome predictions

Guide: TCCATCCCCGGACTCTCCAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TAACCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCC`	(Wild-type sequence)
271	`CTTTCGGCTCTCCATCCCCGGACTCTCCAG--GGGGGGAGGGGGAAAACCCCCAAGTAAG`	`2 bp deleted → frameshift`
219	`CTTTCGGCTCTCCATCCCCGG------------GGGGGAGGGGGAAAACCCCCAAGTAAG`	`12 bp deleted → no frameshift`
213	`CTTTCGGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAG`	`17 bp deleted → frameshift`
208	`CTTTCGGCTCTCCATCCCCGG-------------GGGGAGGGGGAAAACCCCCAAGTAAG`	`13 bp deleted → frameshift`
198	`CTTTCGGCTCTCCATCCCCGG--------------GGGAGGGGGAAAACCCCCAAGTAAG`	`14 bp deleted → frameshift`
188	`CTTTCGGCTCTCCATCCCCGG---------------GGAGGGGGAAAACCCCCAAGTAAG`	`15 bp deleted → no frameshift`
182	`CTTTCGGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAG`	`10 bp deleted → frameshift`
179	`CTTTCGGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAG`	`16 bp deleted → frameshift`
158	`CTTTCGGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAG`	`23 bp deleted → frameshift`
153	`CTTTCGGCTCTCCATCCCC---------------------------------CAAGTAAG`	`33 bp deleted → no frameshift`
147	`CTTTCGGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAG`	`20 bp deleted → frameshift`
146	`CTTTCGGCTCTCCATCCCC----------------------------------AAGTAAG`	`34 bp deleted → frameshift`
143	`CTTTCGGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAG`	`25 bp deleted → frameshift`
140	`CTTTCGGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAG`	`21 bp deleted → no frameshift`
133	`CTTTCGGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAG`	`22 bp deleted → frameshift`
133	`CTTTCGGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAG`	`22 bp deleted → frameshift`
126	`CTTTCGGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAG`	`23 bp deleted → frameshift`
121	`CTTTCGGCTCTCCATCCCC--------------------------------CCAAGTAAG`	`32 bp deleted → frameshift`
120	`CTTTCGGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAG`	`24 bp deleted → no frameshift`
109	`CTTTCGG--------------------------GGGGGAGGGGGAAAACCCCCAAGTAAG`	`26 bp deleted → frameshift`
104	`CTTTCGGCTCTCCATCCC-----------------------------------AAGTAAG`	`35 bp deleted → frameshift`
103	`CTTTCGG---------------------------GGGGAGGGGGAAAACCCCCAAGTAAG`	`27 bp deleted → no frameshift`
98	`CTTTCGG----------------------------GGGAGGGGGAAAACCCCCAAGTAAG`	`28 bp deleted → frameshift`
94	`CTTTCGG-----------------------------GGAGGGGGAAAACCCCCAAGTAAG`	`29 bp deleted → frameshift`
89	`CTTTCGG------------------------------GAGGGGGAAAACCCCCAAGTAAG`	`30 bp deleted → no frameshift`
84	`CTTTCGGCTCTCCATCCCC-------------------------------CCCAAGTAAG`	`31 bp deleted → frameshift`
84	`CTTTCGG-------------------------------AGGGGGAAAACCCCCAAGTAAG`	`31 bp deleted → frameshift`
81	`CTTTCGGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAG`	`26 bp deleted → frameshift`
81	`CTTTCGGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAG`	`26 bp deleted → frameshift`
73	`CTTTCGG----------------------------------GGGAAAACCCCCAAGTAAG`	`34 bp deleted → frameshift`
69	`CTTTCGG-----------------------------------GGAAAACCCCCAAGTAAG`	`35 bp deleted → frameshift`
67	`CTTTCGGCTCTCCA----------------------------------------AGTAAG`	`40 bp deleted → frameshift`
66	`CTTTCGGCTCTCCATCCCCGGACTCTCCAG------------------------------`	`30 bp deleted → no frameshift`
66	`CTTTCGGCTCTCCATCC------------------------------------AAGTAAG`	`36 bp deleted → no frameshift`
66	`CTTTCGG------------------------------------GAAAACCCCCAAGTAAG`	`36 bp deleted → no frameshift`
62	`CTTTCGGCTCTCC-------------------------------------CCCAAGTAAG`	`37 bp deleted → frameshift`
62	`CTTTCGG-------------------------------------AAAACCCCCAAGTAAG`	`37 bp deleted → frameshift`
60	`CTTTCGGCTCTCC--------------------------------------CCAAGTAAG`	`38 bp deleted → frameshift`
56	`CTTTCGGCTCTCC---------------------------------------CAAGTAAG`	`39 bp deleted → no frameshift`

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