CRISPOR

DNA break repair outcome predictions

Guide: GGAGCGTGGCGGGGGAGGCC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGC`	(Wild-type sequence)
603	`CGACTTCCCGGGAGCGTGGCGGGGGAGG--------AGGAGCCGGTCGATGGACTAGCAG`	`8 bp deleted → frameshift`
519	`CGACTTCCCGGGAGCGTGGCGGGGGAGG-----------AGCCGGTCGATGGACTAGCAG`	`11 bp deleted → frameshift`
389	`CGACTTCCCGGGAGCGTGGCGGGGGAGG-----AGGAGGAGCCGGTCGATGGACTAGCAG`	`5 bp deleted → frameshift`
347	`CGACTTCCCGGGAGCGTGGCGGGGGAG--------------CCGGTCGATGGACTAGCAG`	`14 bp deleted → frameshift`
313	`CGACTTCCCGGGAGCGTGGCGGGGGAGGCC-------------GGTCGATGGACTAGCAG`	`13 bp deleted → frameshift`
255	`CGACTTCCCGGGAGCGTGGCGGGG---------AGGAGGAGCCGGTCGATGGACTAGCAG`	`9 bp deleted → no frameshift`
245	`CGACTTCCCGGGAG---------------------GAGGAGCCGGTCGATGGACTAGCAG`	`21 bp deleted → no frameshift`
242	`CGACTTCCCGGGAGCGTGGCGGG----------AGGAGGAGCCGGTCGATGGACTAGCAG`	`10 bp deleted → frameshift`
233	`CGACTTCCCGGGAGC---------------------------CGGTCGATGGACTAGCAG`	`27 bp deleted → no frameshift`
230	`CGACTTCCCGGGAGCGTGGCGG-----------AGGAGGAGCCGGTCGATGGACTAGCAG`	`11 bp deleted → frameshift`
219	`CGACTTCCCGGGAGCGTGGCGGGG------------AGGAGCCGGTCGATGGACTAGCAG`	`12 bp deleted → no frameshift`
210	`CGACTTCCCGGGAG------------------------GAGCCGGTCGATGGACTAGCAG`	`24 bp deleted → no frameshift`
208	`CGACTTCCCGGGAGCGTGGCGGG-------------AGGAGCCGGTCGATGGACTAGCAG`	`13 bp deleted → frameshift`
198	`CGACTTCCCGGGAGCGTGGCGG--------------AGGAGCCGGTCGATGGACTAGCAG`	`14 bp deleted → frameshift`
198	`CGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGATGGACTAGCAG`	`14 bp deleted → frameshift`
190	`CGACTTCCCGGGAGCGTGGCGG-----------------------TCGATGGACTAGCAG`	`23 bp deleted → frameshift`
188	`CGACTTCCCGGGAGCGTGGCGGGG---------------AGCCGGTCGATGGACTAGCAG`	`15 bp deleted → no frameshift`
179	`CGACTTCCCGGGAGCGTGGCGGG----------------AGCCGGTCGATGGACTAGCAG`	`16 bp deleted → frameshift`
170	`CGACTTCCCGGGAGCGTGGCGGGGGAGG-----------------TCGATGGACTAGCAG`	`17 bp deleted → frameshift`
170	`CGACTTCCCGGGAGCGTGGCGG-----------------AGCCGGTCGATGGACTAGCAG`	`17 bp deleted → frameshift`
170	`CGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGATGGACTAGCAG`	`17 bp deleted → frameshift`
147	`CGACTTCCCGGGAGCGTGGCGGGGG--------------------TCGATGGACTAGCAG`	`20 bp deleted → frameshift`
147	`CGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGATGGACTAGCAG`	`20 bp deleted → frameshift`
146	`CGACTTCCCGG----------------------------------TCGATGGACTAGCAG`	`34 bp deleted → frameshift`
140	`CGACTTCCCGGGAGCGTGGCGGGG---------------------TCGATGGACTAGCAG`	`21 bp deleted → no frameshift`
133	`CGACTTCCCGGGAGCGTGGCGGG----------------------TCGATGGACTAGCAG`	`22 bp deleted → frameshift`
133	`CGACTTCCCGGGAGCGTGGC----------------------CGGTCGATGGACTAGCAG`	`22 bp deleted → frameshift`
133	`CGACTTCCCGG----------------------AGGAGGAGCCGGTCGATGGACTAGCAG`	`22 bp deleted → frameshift`
129	`CGACTTCCCGGGAGCGTGGCGGGGGA---------------------------CTAGCAG`	`27 bp deleted → no frameshift`
122	`CGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGATGGACTAGCAG`	`18 bp deleted → no frameshift`
120	`CGACTTCCCGGGAGCGTGGCGGGGGAGG------------------------ACTAGCAG`	`24 bp deleted → no frameshift`
114	`CGACTTCCCGG-------------------------AGGAGCCGGTCGATGGACTAGCAG`	`25 bp deleted → frameshift`
109	`CGACTTCCCGGGAGCGTGG--------------------------TCGATGGACTAGCAG`	`26 bp deleted → frameshift`
103	`CGACTTCCCGGGAGCGTGGCG---------------------------ATGGACTAGCAG`	`27 bp deleted → no frameshift`
98	`CGACTTCCCGGGAGCGTGGCGGGG----------------------------ACTAGCAG`	`28 bp deleted → frameshift`
98	`CGACTTCCCGGGAGCG----------------------------GTCGATGGACTAGCAG`	`28 bp deleted → frameshift`
98	`CGACTTCCCGG----------------------------AGCCGGTCGATGGACTAGCAG`	`28 bp deleted → frameshift`
96	`CGACTTCCCGGGAGCGTGG---------------------------------ACTAGCAG`	`33 bp deleted → no frameshift`
95	`CGACTTCCCGGGAGCGTGGCGGGGGA-----------------------TGGACTAGCAG`	`23 bp deleted → frameshift`
94	`CGACTTCCCGGGAGCGTGGCGGGGGAGGC-----------------------------AG`	`29 bp deleted → frameshift`
94	`CGACTTCCCGGGAGCGTGGCGGG-----------------------------ACTAGCAG`	`29 bp deleted → frameshift`
89	`CGACTTCCCGGGAGCGTGGCGG------------------------------ACTAGCAG`	`30 bp deleted → no frameshift`
80	`CGACTTCCCGGGAGCG--------------------------------ATGGACTAGCAG`	`32 bp deleted → frameshift`
76	`CGACTTCCCGGG---------------------------------TCGATGGACTAGCAG`	`33 bp deleted → no frameshift`
70	`CGACTTCCCGGGAGCGT-----------------------------CGATGGACTAGCAG`	`29 bp deleted → frameshift`
69	`CGACTTCC-----------------------------------GGTCGATGGACTAGCAG`	`35 bp deleted → frameshift`
67	`CGACTTCCCGGGA----------------------------------------CTAGCAG`	`40 bp deleted → frameshift`
66	`CGACTTCCCGGGAGCGTGGCGGGGGAG------------------------------CAG`	`30 bp deleted → no frameshift`
63	`CGA-------------------------------GGAGGAGCCGGTCGATGGACTAGCAG`	`31 bp deleted → frameshift`
60	`CGACTTCCCGGGAGCGTGGC--------------------------------------AG`	`38 bp deleted → frameshift`
60	`CGACTTCCCG--------------------------------------ATGGACTAGCAG`	`38 bp deleted → frameshift`
58	`CGACTTCCCGGGAGC-------------------------------------------AG`	`43 bp deleted → frameshift`
57	`CGACTTCCCGGGAGCGTGGCGGGGGAG---------------------------------`	`33 bp deleted → no frameshift`
54	`CGA----------------------------------GGAGCCGGTCGATGGACTAGCAG`	`34 bp deleted → frameshift`
51	`CGACTTCCCGG-----------------------------------------ACTAGCAG`	`41 bp deleted → frameshift`
50	`CGA----------------------------------------------TGGACTAGCAG`	`46 bp deleted → frameshift`
49	`CGACTTCCCGGGA------------------------------------TGGACTAGCAG`	`36 bp deleted → no frameshift`
49	`CGACT--------------------------------------------------AGCAG`	`50 bp deleted → frameshift`
48	`CG------------------------------------------GTCGATGGACTAGCAG`	`42 bp deleted → no frameshift`
47	`CGA-------------------------------------GCCGGTCGATGGACTAGCAG`	`37 bp deleted → frameshift`
40	`CGACTTC----------------------------------------GATGGACTAGCAG`	`40 bp deleted → frameshift`
30	`CGACTTCCCGGGAG----------------------------------------------`	`46 bp deleted → frameshift`

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