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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| ACCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCA | (Wild-type sequence) | |
| 335 | TTCGGCTCTCCATCCCCGGACTCTCCAGAG--------GGGGAAAACCCCCAAGTAAGCT | 8 bp deleted → frameshift |
| 213 | TTCGGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAGCT | 17 bp deleted → frameshift |
| 208 | TTCGGCTCTCCATCCCCGG-------------GGGGAGGGGGAAAACCCCCAAGTAAGCT | 13 bp deleted → frameshift |
| 198 | TTCGGCTCTCCATCCCCGG--------------GGGAGGGGGAAAACCCCCAAGTAAGCT | 14 bp deleted → frameshift |
| 188 | TTCGGCTCTCCATCCCCGG---------------GGAGGGGGAAAACCCCCAAGTAAGCT | 15 bp deleted → no frameshift |
| 182 | TTCGGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGCT | 10 bp deleted → frameshift |
| 179 | TTCGGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAGCT | 16 bp deleted → frameshift |
| 158 | TTCGGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCT | 23 bp deleted → frameshift |
| 153 | TTCGGCTCTCCATCCCC---------------------------------CAAGTAAGCT | 33 bp deleted → no frameshift |
| 149 | TTCGGCTCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCT | 14 bp deleted → frameshift |
| 147 | TTCGGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCT | 20 bp deleted → frameshift |
| 146 | TTCGGCTCTCCATCCCC----------------------------------AAGTAAGCT | 34 bp deleted → frameshift |
| 143 | TTCGGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAGCT | 25 bp deleted → frameshift |
| 140 | TTCGGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCT | 21 bp deleted → no frameshift |
| 133 | TTCGGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCT | 22 bp deleted → frameshift |
| 133 | TTCGGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCT | 22 bp deleted → frameshift |
| 126 | TTCGGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCT | 23 bp deleted → frameshift |
| 121 | TTCGGCTCTCCATCCCC--------------------------------CCAAGTAAGCT | 32 bp deleted → frameshift |
| 120 | TTCGGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAGCT | 24 bp deleted → no frameshift |
| 104 | TTCGGCTCTCCATCCC-----------------------------------AAGTAAGCT | 35 bp deleted → frameshift |
| 103 | TTCGG---------------------------GGGGAGGGGGAAAACCCCCAAGTAAGCT | 27 bp deleted → no frameshift |
| 98 | TTCGG----------------------------GGGAGGGGGAAAACCCCCAAGTAAGCT | 28 bp deleted → frameshift |
| 94 | TTCGG-----------------------------GGAGGGGGAAAACCCCCAAGTAAGCT | 29 bp deleted → frameshift |
| 90 | TTCGGCTCTCCATCCCCGGACTCTCCAGAG------------------------TAAGCT | 24 bp deleted → no frameshift |
| 89 | TTCGG------------------------------GAGGGGGAAAACCCCCAAGTAAGCT | 30 bp deleted → no frameshift |
| 84 | TTCGGCTCTCCATCCCC-------------------------------CCCAAGTAAGCT | 31 bp deleted → frameshift |
| 84 | TTCGG-------------------------------AGGGGGAAAACCCCCAAGTAAGCT | 31 bp deleted → frameshift |
| 81 | TTCGGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAGCT | 26 bp deleted → frameshift |
| 81 | TTCGGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAGCT | 26 bp deleted → frameshift |
| 74 | TTCGGCTCTCCATCCCCGGACTCTCCAGAG----------------------------CT | 28 bp deleted → frameshift |
| 73 | TTCGG----------------------------------GGGAAAACCCCCAAGTAAGCT | 34 bp deleted → frameshift |
| 69 | TTCGG-----------------------------------GGAAAACCCCCAAGTAAGCT | 35 bp deleted → frameshift |
| 67 | TTCGGCTCTCCA----------------------------------------AGTAAGCT | 40 bp deleted → frameshift |
| 66 | TTCGGCTCTCCATCCCCGGACTCTCCAG------------------------------CT | 30 bp deleted → no frameshift |
| 66 | TTCGGCTCTCCATCC------------------------------------AAGTAAGCT | 36 bp deleted → no frameshift |
| 66 | TTCGG------------------------------------GAAAACCCCCAAGTAAGCT | 36 bp deleted → no frameshift |
| 62 | TTCGGCTCTCC-------------------------------------CCCAAGTAAGCT | 37 bp deleted → frameshift |
| 62 | TTCGG-------------------------------------AAAACCCCCAAGTAAGCT | 37 bp deleted → frameshift |
| 60 | TTCGGCTCTCC--------------------------------------CCAAGTAAGCT | 38 bp deleted → frameshift |
| 56 | TTCGGCTCTCC---------------------------------------CAAGTAAGCT | 39 bp deleted → no frameshift |
| 49 | TTCGGCTCTCCATCCCCGGACTCT------------------------------------ | 36 bp deleted → no frameshift |
| 45 | TTCGGCTCTCCATCCCCGGACT-------------------------------------- | 38 bp deleted → frameshift |
| 35 | TTCGGCT----------------------------------------------------- | 53 bp deleted → frameshift |
| 23 | TTCGGCTCT--------------------------------------------------- | 51 bp deleted → no frameshift |