CRISPOR

DNA break repair outcome predictions

Guide: CGGAGGGGACTGGGGGTCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGCTCGGCGC`	(Wild-type sequence)
423	`AGGCTGTCCGCGGAGGGGACTGGGGG-------CCAAGCATGGCCGCTCCAGCAGACCGT`	`7 bp deleted → frameshift`
402	`AGGCTGTCCGCGGAGGGGACTGGGG--------CCAAGCATGGCCGCTCCAGCAGACCGT`	`8 bp deleted → frameshift`
382	`AGGCTGTCCGCGGAGGGGACTGGG---------CCAAGCATGGCCGCTCCAGCAGACCGT`	`9 bp deleted → no frameshift`
311	`AGGCTGTCCGCGGAGGGGACTGGGGGTCCC-----AAGCATGGCCGCTCCAGCAGACCGT`	`5 bp deleted → frameshift`
296	`AGGCTGTCCGCGGAGGGGACTGGGGGTCC------AAGCATGGCCGCTCCAGCAGACCGT`	`6 bp deleted → no frameshift`
296	`AGGCTGTCCGCGGAGGGGACTGGGGG------GCCAAGCATGGCCGCTCCAGCAGACCGT`	`6 bp deleted → no frameshift`
283	`AGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCCAGCAGACCGT`	`15 bp deleted → no frameshift`
269	`AGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCCAGCAGACCGT`	`16 bp deleted → frameshift`
242	`AGGCTGTCCGCGGAGGGGACTGG----------CCAAGCATGGCCGCTCCAGCAGACCGT`	`10 bp deleted → frameshift`
198	`AGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCCAGCAGACCGT`	`14 bp deleted → frameshift`
188	`AGGCTGTCCGCGGAGGGGACTGGGGGTCCC---------------GCTCCAGCAGACCGT`	`15 bp deleted → no frameshift`
184	`AGGCTGTCCGCGGAGGGGACTGG--------------------CCGCTCCAGCAGACCGT`	`20 bp deleted → frameshift`
179	`AGGCTGTCCGCGGAGGGGACTGGGGGTCC----------------GCTCCAGCAGACCGT`	`16 bp deleted → frameshift`
175	`AGGCTGTCCGCGGAGGGGACTGGGGGTCC---------------------AGCAGACCGT`	`21 bp deleted → no frameshift`
170	`AGGCTGTCCGCGGAGGGGACTGGGGG-----------------CCGCTCCAGCAGACCGT`	`17 bp deleted → frameshift`
170	`AGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCCAGCAGACCGT`	`17 bp deleted → frameshift`
162	`AGGCTGTCCGCGGAGGGGACTGGGG------------------CCGCTCCAGCAGACCGT`	`18 bp deleted → no frameshift`
154	`AGGCTGTCCGCGGAGGGGACTGGG-------------------CCGCTCCAGCAGACCGT`	`19 bp deleted → frameshift`
154	`AGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCCAGCAGACCGT`	`19 bp deleted → frameshift`
147	`AGGCTGTCCGCGGAGGGGACTGGGGGTCCC--------------------AGCAGACCGT`	`20 bp deleted → frameshift`
147	`AGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCCAGCAGACCGT`	`20 bp deleted → frameshift`
133	`AGGC------------------------------CAAGCATGGCCGCTCCAGCAGACCGT`	`30 bp deleted → no frameshift`
132	`AGGCTGTCCGC------------------------------------TCCAGCAGACCGT`	`36 bp deleted → no frameshift`
126	`AGGCTGTCCGC-----------------------CAAGCATGGCCGCTCCAGCAGACCGT`	`23 bp deleted → frameshift`
114	`AGGCTGTCCGCGGAGGGG-------------------------CCGCTCCAGCAGACCGT`	`25 bp deleted → frameshift`
109	`AGGCTGTCCGCGGAGGG--------------------------CCGCTCCAGCAGACCGT`	`26 bp deleted → frameshift`
109	`AGGCTGTCC--------------------------AAGCATGGCCGCTCCAGCAGACCGT`	`26 bp deleted → frameshift`
103	`AGGCTGTCCGCGGAGG---------------------------CCGCTCCAGCAGACCGT`	`27 bp deleted → no frameshift`
98	`AGGCTGTCCGCGGAGGGGACTGGGGGTCCC----------------------------GT`	`28 bp deleted → frameshift`
98	`AGGCTGTCCGC----------------------------ATGGCCGCTCCAGCAGACCGT`	`28 bp deleted → frameshift`
95	`AGGCTGTCCGCGGAG-----------------------CATGGCCGCTCCAGCAGACCGT`	`23 bp deleted → frameshift`
94	`AGGCTGTCCGCGGAGGGGACTGGGGGTCC-----------------------------GT`	`29 bp deleted → frameshift`
94	`AGG-----------------------------GCCAAGCATGGCCGCTCCAGCAGACCGT`	`29 bp deleted → frameshift`
89	`AGGCTGTCCGCGG------------------------------CCGCTCCAGCAGACCGT`	`30 bp deleted → no frameshift`
81	`AGGC----------------------------------------CGCTCCAGCAGACCGT`	`40 bp deleted → frameshift`
78	`AGGCTGTCCGCGGAGGGGAC-------------------------------------CGT`	`37 bp deleted → frameshift`
77	`AGGCTGTCCGCGGAGGGGACT---------------------------CCAGCAGACCGT`	`27 bp deleted → no frameshift`
76	`AGGCTGTCCGC---------------------------------CGCTCCAGCAGACCGT`	`33 bp deleted → no frameshift`
73	`AGGCTGTCCGCG----------------------------------CTCCAGCAGACCGT`	`34 bp deleted → frameshift`
69	`AGGC-----------------------------------ATGGCCGCTCCAGCAGACCGT`	`35 bp deleted → frameshift`
64	`AGGCTGTCC-----------------------------------------AGCAGACCGT`	`41 bp deleted → frameshift`
58	`AGGCT-------------------------------------------CCAGCAGACCGT`	`43 bp deleted → frameshift`
57	`AGGCTGTCCGCGGAGGGGACTGGGGGT---------------------------------`	`33 bp deleted → no frameshift`
51	`AGGCTGTCCG-------------------------------------------------T`	`49 bp deleted → frameshift`
49	`AGGCTG------------------------------------GCCGCTCCAGCAGACCGT`	`36 bp deleted → no frameshift`
49	`AG------------------------------------CATGGCCGCTCCAGCAGACCGT`	`36 bp deleted → no frameshift`
48	`AGGCTGTCCGC------------------------------------------AGACCGT`	`42 bp deleted → no frameshift`
47	`AGGCTGTCCGCGGAG-------------------------------------CAGACCGT`	`37 bp deleted → frameshift`
40	`AGGCTGTCCGCGGAG----------------------------------------ACCGT`	`40 bp deleted → frameshift`
38	`AGGCTGTCCGCG-----------------------------------------------T`	`47 bp deleted → frameshift`
36	`AGGCTGTCCGCGGA------------------------------------------CCGT`	`42 bp deleted → no frameshift`
34	`AGGC-------------------------------------------------AGACCGT`	`49 bp deleted → frameshift`
24	`AG--------------------------------------------------CAGACCGT`	`50 bp deleted → frameshift`
21	`AGGCTGT-----------------------------------------------------`	`53 bp deleted → frameshift`
21	`AG-----------------------------------------------------ACCGT`	`53 bp deleted → frameshift`

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