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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314089_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314089_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm3_intergenic_Gm12036|Aftph_chr11_20733209_F | Primer3: not found at this Tm | N.d. | 0.60 |
| mm3_intergenic_Gm12036|Aftph_chr11_20733209_R | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4_intron_Bcl11b_chr12_107934038_F | TCGTCGGCAGCGTCTGGAGCCAAAGCACAAGAGT | 59.8 | 0.43 |
| mm4_intron_Bcl11b_chr12_107934038_R | GTCTCGTGGGCTCGGTTTCTCCTTCTGCGCTCTCC | 59.7 | 0.43 |
| mm3_intron_Npas3_chr12_53562882_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm3_intron_Npas3_chr12_53562882_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_29570537_F | TCGTCGGCAGCGTCTCACGGTCACCATTCTAATCAGT | 59.4 | 0.35 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_29570537_R | GTCTCGTGGGCTCGGTTCAGGTGCACGTTTCACCT | 60.1 | 0.35 |
| mm3_intergenic_Prkg1|A1cf_chr19_31829513_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm3_intergenic_Prkg1|A1cf_chr19_31829513_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Fscb|Gm24066_chr12_64588533_F | TCGTCGGCAGCGTCACCCCTTCTTTTCTCGTTGCT | 59.8 | 0.29 |
| mm4_intergenic_Fscb|Gm24066_chr12_64588533_R | GTCTCGTGGGCTCGGGCATAGGTACATTTCTGCTTCGA | 58.8 | 0.29 |
| mm4_intron_Dhrs3_chr4_144909557_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Dhrs3_chr4_144909557_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm22802|Slitrk5_chr14_111492822_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Gm22802|Slitrk5_chr14_111492822_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Sytl3_chr17_6719336_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Sytl3_chr17_6719336_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Hba-ps4|Neurl1b_chr17_26339059_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Hba-ps4|Neurl1b_chr17_26339059_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm26848|Dynlt1c_chr17_6532106_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm26848|Dynlt1c_chr17_6532106_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Tcof1|Arsi_chr18_60869690_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Tcof1|Arsi_chr18_60869690_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Myh6_chr14_54941957_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Myh6_chr14_54941957_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Asb4_chr6_5416514_F | TCGTCGGCAGCGTCGCCTAGGCTTACACTCCGTC | 59.8 | 0.21 |
| mm4_intron_Asb4_chr6_5416514_R | GTCTCGTGGGCTCGGGCACAACCTGCAATACCAGC | 60.1 | 0.21 |
| mm4_exon_Sptbn4_chr7_27414727_F | TCGTCGGCAGCGTCAATCTCAGGGCTGCAGCATT | 60.0 | 0.21 |
| mm4_exon_Sptbn4_chr7_27414727_R | GTCTCGTGGGCTCGGCAGACTCAGCTGCTGTCTCG | 60.4 | 0.21 |
| mm4_intergenic_Efr3b|Dnajc27_chr12_4054937_F | TCGTCGGCAGCGTCAGCATGCAGGACAGAGTCAC | 60.0 | 0.21 |
| mm4_intergenic_Efr3b|Dnajc27_chr12_4054937_R | GTCTCGTGGGCTCGGGGAGAGGCTGTGAGATGCTG | 60.1 | 0.21 |
| mm4_intergenic_Asb1|Twist2_chr1_91571563_F | TCGTCGGCAGCGTCCCTTGGTCAGGTCAGTGCTT | 59.8 | 0.21 |
| mm4_intergenic_Asb1|Twist2_chr1_91571563_R | GTCTCGTGGGCTCGGGCCTCTCCAAAGCACCAAAG | 59.4 | 0.21 |
| mm4_exon_Rnf14_chr18_38297176_F | TCGTCGGCAGCGTCGCCGGGAAGATGAATCGGAT | 59.9 | 0.20 |
| mm4_exon_Rnf14_chr18_38297176_R | GTCTCGTGGGCTCGGAGCTACTGTTGCAAACCGGA | 59.8 | 0.20 |
| mm4_intergenic_Usp54/1810062O18Rik|Usp54_chr14_20570950_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Usp54/1810062O18Rik|Usp54_chr14_20570950_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm4868|Gm23151_chr5_126273334_F | TCGTCGGCAGCGTCGGCACAACACAACCCAGAAA | 59.1 | 0.18 |
| mm4_intergenic_Gm4868|Gm23151_chr5_126273334_R | GTCTCGTGGGCTCGGGGAATTGTGCCACTGCGAAC | 60.3 | 0.18 |
| mm4_exon_Rgs13_chr1_144140708_F | TCGTCGGCAGCGTCGTCTTCCAAGTGATGCCCCA | 59.9 | 0.17 |
| mm4_exon_Rgs13_chr1_144140708_R | GTCTCGTGGGCTCGGGGGGCAGAATTTCTAGGGCA | 59.7 | 0.17 |
| mm4_intron_Atp10a_chr7_58677533_F | TCGTCGGCAGCGTCCCCCAAAACCAAGAAGCCCA | 60.7 | 0.17 |
| mm4_intron_Atp10a_chr7_58677533_R | GTCTCGTGGGCTCGGGCTCTGAGTTCCCCTAGTTAGC | 59.8 | 0.17 |
| mm4_intron_Best3_chr10_117005874_F | TCGTCGGCAGCGTCGCCTGGCTCTGTTCGAGATA | 59.2 | 0.17 |
| mm4_intron_Best3_chr10_117005874_R | GTCTCGTGGGCTCGGGGAGGCCCCATTTGCAGTAA | 60.3 | 0.17 |
| mm4_intergenic_Gm9992|Gm25872_chr17_7582773_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm9992|Gm25872_chr17_7582773_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Spock1_chr13_57541003_F | TCGTCGGCAGCGTCTCACCACCGAGTAAAAGACCA | 58.9 | 0.16 |
| mm4_intron_Spock1_chr13_57541003_R | GTCTCGTGGGCTCGGGACAGCAGCAGGGAACTCTT | 59.9 | 0.16 |
| mm4_intron_Lrch3_chr16_32974534_F | TCGTCGGCAGCGTCGGACTGGACACTGATGGGTG | 60.0 | 0.15 |
| mm4_intron_Lrch3_chr16_32974534_R | GTCTCGTGGGCTCGGGAACTCACTGAGCTGTGCCA | 60.2 | 0.15 |
| mm4_intergenic_Gdf15|Pgpep1_chr8_70636867_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gdf15|Pgpep1_chr8_70636867_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Gm17212|Gm17213_chr3_69374680_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Gm17212|Gm17213_chr3_69374680_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Trmt1_chr8_84690910_F | TCGTCGGCAGCGTCATGAAAATCTGGCCCCAGGG | 60.0 | 0.13 |
| mm4_exon_Trmt1_chr8_84690910_R | GTCTCGTGGGCTCGGCACTTCTTTCTGAGGCCCGT | 59.9 | 0.13 |
| mm4_intron_Pdcd6_chr13_74304481_F | TCGTCGGCAGCGTCCATCATGCAGGGATCAGCCT | 59.8 | 0.13 |
| mm4_intron_Pdcd6_chr13_74304481_R | GTCTCGTGGGCTCGGTGCTCTTCGACTTCTGACCTG | 59.7 | 0.13 |
| mm4_exon_Tonsl_chr15_76639663_F | TCGTCGGCAGCGTCGCCTCCAGCTAGTCCTCTCA | 60.3 | 0.13 |
| mm4_exon_Tonsl_chr15_76639663_R | GTCTCGTGGGCTCGGCCCAGAGGAATGGACAGCTG | 60.1 | 0.13 |
| mm4_intron_Zfp536_chr7_37728130_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Zfp536_chr7_37728130_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Cdk19|Slc22a16_chr10_40499822_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Cdk19|Slc22a16_chr10_40499822_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Cnnm2|Nt5c2_chr19_46879693_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Cnnm2|Nt5c2_chr19_46879693_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intron_Acvr1_chr2_58550494_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intron_Acvr1_chr2_58550494_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm2_intron_Podn_chr4_108020270_F | TCGTCGGCAGCGTCTGCAGGTATTGTGCTTCCAGT | 59.9 | 0.11 |
| mm2_intron_Podn_chr4_108020270_R | GTCTCGTGGGCTCGGGGGGTTTCAAGCCTCTGTCT | 59.5 | 0.11 |
| mm4_exon_Gm15676_chr7_126808089_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Gm15676_chr7_126808089_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_2310065F04Rik|Myh2_chr11_67143408_F | TCGTCGGCAGCGTCTCTCTCAGCAGAGGTTGGGA | 59.8 | 0.10 |
| mm4_intergenic_2310065F04Rik|Myh2_chr11_67143408_R | GTCTCGTGGGCTCGGTGGAAGTTGCTCCTGTTGCT | 59.8 | 0.10 |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191337_F | TCGTCGGCAGCGTCTGAATGTCTGATCTTCTGATAGGGA | 58.8 | 0.10 |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191337_R | GTCTCGTGGGCTCGGAGCATAGCAAGAAGAATGGAGCT | 60.1 | 0.10 |
| mm4_intergenic_Nkx2-2os|AI646519_chr2_147352640_F | TCGTCGGCAGCGTCGCCTTTCCCTCTCTGAACCC | 60.0 | 0.10 |
| mm4_intergenic_Nkx2-2os|AI646519_chr2_147352640_R | GTCTCGTGGGCTCGGTGGGTAGTGACAAGCAAGGG | 59.6 | 0.10 |
| mm4_exon_Baiap3_chr17_25245947_F | TCGTCGGCAGCGTCCCTCTCACCTGCTCACTCAC | 59.7 | 0.09 |
| mm4_exon_Baiap3_chr17_25245947_R | GTCTCGTGGGCTCGGACACGGAACTGCTGAGGAAG | 59.9 | 0.09 |
| mm4_intron_Kcnn3_chr3_89610740_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Kcnn3_chr3_89610740_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Zmiz1|Mir3075_chr14_25533150_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Zmiz1|Mir3075_chr14_25533150_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Il1rap_chr16_26582218_F | TCGTCGGCAGCGTCGGTCCTTGTGCCTAGGGTTC | 60.0 | 0.08 |
| mm4_intron_Il1rap_chr16_26582218_R | GTCTCGTGGGCTCGGTCTCCCCAGGGCTGTCATAG | 60.3 | 0.08 |
| mm4_intron_1700101E01Rik_chr2_29003080_F | TCGTCGGCAGCGTCACATACACACAAACAGCCCC | 58.3 | 0.08 |
| mm4_intron_1700101E01Rik_chr2_29003080_R | GTCTCGTGGGCTCGGCACACTGAGGTACAAGGGCC | 60.3 | 0.08 |
| mm4_intergenic_Ppp2r5c|Dync1h1_chr12_110595890_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ppp2r5c|Dync1h1_chr12_110595890_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Chn2_chr6_54040215_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Chn2_chr6_54040215_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Slc1a2_chr2_102664672_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Slc1a2_chr2_102664672_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_exon_Nsmce1_chr7_125486383_F | TCGTCGGCAGCGTCGGACACAGCACTTACGGTCA | 59.9 | 0.06 |
| mm3_exon_Nsmce1_chr7_125486383_R | GTCTCGTGGGCTCGGCAGGAGCCATGACGGATGTG | 60.8 | 0.06 |
| mm4_intergenic_Gm8521|Gm20918_chrY_4350062_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm8521|Gm20918_chrY_4350062_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm8521|Gm20918_chrY_4234749_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm8521|Gm20918_chrY_4234749_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Asxl1_chr2_153394046_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Asxl1_chr2_153394046_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20851|Gm21660_chrY_18503873_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20851|Gm21660_chrY_18503873_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20795|Gm20827_chrY_40927909_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20795|Gm20827_chrY_40927909_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20773|Gm20836_chrY_13791150_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20773|Gm20836_chrY_13791150_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Grhl3_chr4_135573352_F | TCGTCGGCAGCGTCCTTGCTCTTCCCTCTGCAGG | 60.3 | 0.05 |
| mm4_intron_Grhl3_chr4_135573352_R | GTCTCGTGGGCTCGGTAAAGCCACGGATGTCTGGC | 60.3 | 0.05 |
| mm4_intron_Dtd1_chr2_144697911_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Dtd1_chr2_144697911_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm11737|Cyth1_chr11_118206273_F | TCGTCGGCAGCGTCCTCACCTGCTAACCCCATCC | 59.8 | 0.04 |
| mm4_intergenic_Gm11737|Cyth1_chr11_118206273_R | GTCTCGTGGGCTCGGGCTCACTCTTGTCCAAGGCT | 59.9 | 0.04 |
| mm4_intron_Gm2694_chr8_87512797_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm2694_chr8_87512797_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Setbp1|Gm25824_chr18_79128573_F | TCGTCGGCAGCGTCGCAGACAGAGATCAGAGGGG | 59.2 | 0.03 |
| mm4_intergenic_Setbp1|Gm25824_chr18_79128573_R | GTCTCGTGGGCTCGGTGTGTCTGCTTCCCAGTTCC | 59.8 | 0.03 |
| mm3_intergenic_Gm24696|Myc_chr15_61864783_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm24696|Myc_chr15_61864783_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Plekha7|Rps13_chr7_116319087_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Plekha7|Rps13_chr7_116319087_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Fam78b_chr1_167008158_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Fam78b_chr1_167008158_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Whrn_chr4_63424929_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Whrn_chr4_63424929_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Wipf1_chr2_73500718_F | TCGTCGGCAGCGTCATCTCTTCTTGCCCGGGTTC | 59.7 | 0.01 |
| mm4_intron_Wipf1_chr2_73500718_R | GTCTCGTGGGCTCGGCATCTTTGCCGCGCATAAGT | 59.6 | 0.01 |
| mm4_intron_Snph_chr2_151620529_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Snph_chr2_151620529_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm24254|4933436C20Rik_chr8_92049634_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm24254|4933436C20Rik_chr8_92049634_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Mir3092|Fndc3b_chr3_27615620_F | TCGTCGGCAGCGTCTGAAAAGAGATGGCAGCCAGT | 59.9 | 0.01 |
| mm4_intergenic_Mir3092|Fndc3b_chr3_27615620_R | GTCTCGTGGGCTCGGTGAGCAGGATTCACGTTGGA | 59.3 | 0.01 |
| mm4_intergenic_Gm22032|Gm22895_chr15_17593025_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22032|Gm22895_chr15_17593025_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314089 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intron_Bcl11b_chr12_107934038 |
TGGAGCCAAAGCACAAGAGTGAGGCTTACTTAACGAACACCATTGACAGGGTTTCTAGTGGAACGGAGCCCGCCCTTCCC ACTCTAGCTGGCCGTGGCTCAGAATCTCTCCCTGGAGAGCGCAGAAGGAGAAA |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_29570537 |
TCACGGTCACCATTCTAATCAGTCCTCTCCAGCAGACCATGACTAATACAGTGAATAGGCTTTTGCTGCGAAAATTTGGT GACTGATTTAATATCATCAGGTGAAACGTGCACCTGAA |
| mm4_intergenic_Fscb|Gm24066_chr12_64588533 |
ACCCCTTCTTTTCTCGTTGCTAATCAAAAATGATCATACTTGCCAACTGGAGGTTATGAGACCTCTCCAGCAGACCATAG CGTGGCAGAAAAAAGGCTGAATATATTGTAGCTACTTTCGAAGCAGAAATGTACCTATGC |
| mm4_intron_Asb4_chr6_5416514 |
GCCTAGGCTTACACTCCGTCCGCCTCTCCAGCTGACCTTGTCTTGTGCTCCCCATCCTCAAGGAGCTGGTATTGCAGGTT GTGC |
| mm4_exon_Sptbn4_chr7_27414727 |
AATCTCAGGGCTGCAGCATTGAGGGCCTCCACTCGCTCACTCTGGGCTGCGATGTCCCCCTCCAGCAGCCCGTGCTTCTG CAGCAGGTCATCGGCCTCCACCAGGTGCTGCCCACATTCCCGAGACAGCAGCTGAGTCTG |
| mm4_intergenic_Efr3b|Dnajc27_chr12_4054937 |
AGCATGCAGGACAGAGTCACCAGATCACCTCGCTTTCTCCCCCTCCCCTCCAGCACTCCCTGGCTCAGGCTCACAGCATC TCACAGCCTCTCC |
| mm4_intergenic_Asb1|Twist2_chr1_91571563 |
CCTTGGTCAGGTCAGTGCTTTGTGAGCCTGGAAAATCCACATGACTCTTTGGTAGGGATTGTCACAGTACTAAGAATGGG ACTTGAACTATGGAAAGAGCCCCTGTCTGCAGGAGTGGCTTTGGTGCTTTGGAGAGGC |
| mm4_exon_Rnf14_chr18_38297176 |
GCCGGGAAGATGAATCGGATTTTCCCAAGCTAGGAAAACCCGGCGGAGGAGGGGCGTCACGTTCTGCTGGAGTGGCTTCC GGTTTGCAACAGTAGCT |
| mm4_intergenic_Gm4868|Gm23151_chr5_126273334 |
GGCACAACACAACCCAGAAATAGATTTTTTTTTTTCCTGTTTCAGCCACGGGCTGCTGTAGAGGTGGCAGCATTGTTAGT TACCAGATTCTAAAATAGACTTGGTACGTTCGCAGTGGCACAATTCC |
| mm4_exon_Rgs13_chr1_144140708 |
GTCTTCCAAGTGATGCCCCAACTGCCATGTCCCATTGTTACCTCTCTAGGAGACTGTGGCTGGATGTAGATATTGTAAAG CTTTTTTGCCCTAGAAATTCTGCCCC |
| mm4_intron_Atp10a_chr7_58677533 |
CCCCAAAACCAAGAAGCCCAATGTCACTTCTGTGACAGAATACCAGACTTCTTATACTTGAGTCCCCTCCAGCACTCAGT GGCTCAGAAAATGTGCTAACTAGGGGAACTCAGAGC |
| mm4_intron_Best3_chr10_117005874 |
GCCTGGCTCTGTTCGAGATAAGCCAGGGTAATAAAAGTGCAGTGCCCACACCAGCAGACCGTGTCCCCGTTTCCAGAGAA CTGCTCTATCCAAAGTGTAGGAAACACGCCTTGATCTTTCTTACTGCAAATGGGGCCTCC |
| mm4_intron_Spock1_chr13_57541003 |
TCACCACCGAGTAAAAGACCATTCTTACCCCAGCCAAGGACTGCTGCAGAGGATGTCCTCAATAAGAGTTCCCTGCTGCT GTC |
| mm4_intron_Lrch3_chr16_32974534 |
GGACTGGACACTGATGGGTGAGGAAGGGGAACCTGAGCTTTAAGTGACAGCTCAAAAGTGGAGTCCTCTCCAGCAGGCAC TGGCTGGAGCCCCACAGAAGGTGGCACAGCTCAGTGAGTTC |
| mm4_exon_Trmt1_chr8_84690910 |
ATGAAAATCTGGCCCCAGGGGACTGGCCACGGACTGCTGCAGTGGGTGAGATCTGTGAGGTACGGGCCTCAGAAAGAAGT G |
| mm4_intron_Pdcd6_chr13_74304481 |
CATCATGCAGGGATCAGCCTTTATAGCCCACTTCCCCTCCAGCAGACTTTGGATCCAGACAGGTCAGAAGTCGAAGAGCA |
| mm4_exon_Tonsl_chr15_76639663 |
GCCTCCAGCTAGTCCTCTCACCGCTCCGGCTCACCGTGGCTAGCCAGCAGCTCCCCCAGCTGGTGGCAGTAGGCCGCCTC CTCGCGCAGCTGTCCATTCCTCTGGG |
| mm2_intron_Podn_chr4_108020270 | TGCAGGTATTGTGCTTCCAGTAGAAGAGCCACGGTGTGCTGGAGCAGGAGCAGACAGAGGCTTGAAACCCC |
| mm4_intergenic_2310065F04Rik|Myh2_chr11_67143408 |
TCTCTCAGCAGAGGTTGGGAACAAGTAAGGAGCCAAGGGGTGCTGGAGGGGTATCTCCCCCCACCCCCAAGGTGCACTTC CTGCCTCTTATTTAATAAGCCACAGCAACAGGAGCAACTTCCA |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191337 |
TGAATGTCTGATCTTCTGATAGGGATACTTACTCCTTTTAATGAAGACCAAGCCAAGGTCTTCTGGATAGGCTATATGCT TTAAGAACTAAGCTCCATTCTTCTTGCTATGCT |
| mm4_intergenic_Nkx2-2os|AI646519_chr2_147352640 |
GCCTTTCCCTCTCTGAACCCTGATGTCTCAGTGTTGAGCTGACGTAGAAGGAGGCATGTTCTGCTGGAGAAGCTCAAACT CATGAAAACTGAAGGTGAACAAAAGGGGTTCTACTCACAGCCCTTGCTTGTCACTACCCA |
| mm4_exon_Baiap3_chr17_25245947 | CCTCTCACCTGCTCACTCACTGCCTCACCAGCAGCCCCTGGCTGGGTGTCCACCTTCTTCCTCAGCAGTTCCGTGT |
| mm4_intron_Il1rap_chr16_26582218 |
GGTCCTTGTGCCTAGGGTTCTCAGGAGTGCCGCTGTAAGCTTGAGGCGAGCAGGAGGCTGTGTCCCAGCACACCCACTCC ATCACATCGTAGCTCGCAGTCCCAGCTATGACAGCCCTGGGGAGA |
| mm4_intron_1700101E01Rik_chr2_29003080 |
ACATACACACAAACAGCCCCCACCCCCAGTGCAGCAGACCCTGGCTAGAAAAAACTGAGACGTGTAGAGCACAGTAGGTC CATAGATCCCAGGCGGCGGGGGCCCTTGTACCTCAGTGTG |
| mm3_exon_Nsmce1_chr7_125486383 |
GGACACAGCACTTACGGTCATGGACCTGGTAGCAGTGGTTCTGCAGACGCCGAACCTCCCACTCCTCCAGCACACCGTGG GTCATGAGCAGCTGGAGGAAGCGCCGGTGCACATCCGTCATGGCTCCTG |
| mm4_intron_Grhl3_chr4_135573352 | CTTGCTCTTCCCTCTGCAGGGAATCCCAGCCAGCCTCGGTGTGCTGGGGAGGCCGGGAGGCCAGACATCCGTGGCTTTA |
| mm4_intergenic_Gm11737|Cyth1_chr11_118206273 | CTCACCTGCTAACCCCATCCCTCTCTCCTGTCAGGCACACGGTCTGCTGGAGCAGAGAAGCCTTGGACAAGAGTGAGC |
| mm4_intergenic_Setbp1|Gm25824_chr18_79128573 |
GCAGACAGAGATCAGAGGGGGGAATGACATCACCATGACGTCACCAGCCACGGGCTGCGGGGGGGGGGGGGGGGCAGTCA GCTCGTGCCCTGAGGGGCGCTGTGAAGGAACTGGGAAGCAGACACA |
| mm4_intron_Wipf1_chr2_73500718 |
ATCTCTTCTTGCCCGGGTTCTGAGTCCACGCTCCACGGTCTTCTGGGGTGGATATCTCTAGGCTTCTTTTACAAACAAGG AAACTGAGTTTATGGCAAATTAACAGGTAAAGGAGCCAAAACTTATGCGCGGCAAAGATG |
| mm4_intergenic_Mir3092|Fndc3b_chr3_27615620 |
TGAAAAGAGATGGCAGCCAGTGCTTCACCTAACAAATACTCTGAAAGGACAAATGCTTTGGAAGAAACAGTAGGTTCTGC AGGCTGAGCCACGGCCTGGGGGAGGGGACAAATGAGGAAATCCAACGTGAATCCTGCTCA |