CRISPOR

DNA break repair outcome predictions

Guide: TCCCGGGAGCGTGGCGGGGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGT`	(Wild-type sequence)
382	`CCCTCCGACTTCCCGGGAGCGTGGC---------CAGGAGGAGGAGCCGGTCGATGGACT`	`9 bp deleted → no frameshift`
344	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG---CCAGGAGGAGGAGCCGGTCGATGGACT`	`3 bp deleted → no frameshift`
327	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG----CCAGGAGGAGGAGCCGGTCGATGGACT`	`4 bp deleted → frameshift`
311	`CCCTCCGACTTCCCGGGAGCGTGGCGGG-----CCAGGAGGAGGAGCCGGTCGATGGACT`	`5 bp deleted → frameshift`
296	`CCCTCCGACTTCCCGGGAGCGTGGCGG------CCAGGAGGAGGAGCCGGTCGATGGACT`	`6 bp deleted → no frameshift`
268	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG--------AGGAGGAGCCGGTCGATGGACT`	`8 bp deleted → frameshift`
255	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG---------AGGAGGAGCCGGTCGATGGACT`	`9 bp deleted → no frameshift`
245	`CCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGTCGATGGACT`	`21 bp deleted → no frameshift`
242	`CCCTCCGACTTCCCGGGAGCGTGGCGGG----------AGGAGGAGCCGGTCGATGGACT`	`10 bp deleted → frameshift`
233	`CCCTCCGACTTCCCGGGAGC---------------------------CGGTCGATGGACT`	`27 bp deleted → no frameshift`
230	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG-----------AGGAGCCGGTCGATGGACT`	`11 bp deleted → frameshift`
230	`CCCTCCGACTTCCCGGGAGCGTGGCGG-----------AGGAGGAGCCGGTCGATGGACT`	`11 bp deleted → frameshift`
219	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG------------AGGAGCCGGTCGATGGACT`	`12 bp deleted → no frameshift`
210	`CCCTCCGACTTCCCGGGAG------------------------GAGCCGGTCGATGGACT`	`24 bp deleted → no frameshift`
208	`CCCTCCGACTTCCCGGGAGCGTGGCGGG-------------AGGAGCCGGTCGATGGACT`	`13 bp deleted → frameshift`
198	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG--------------AGCCGGTCGATGGACT`	`14 bp deleted → frameshift`
198	`CCCTCCGACTTCCCGGGAGCGTGGCGG--------------AGGAGCCGGTCGATGGACT`	`14 bp deleted → frameshift`
198	`CCCTCCGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGATGGACT`	`14 bp deleted → frameshift`
198	`CCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGTCGATGGACT`	`14 bp deleted → frameshift`
190	`CCCTCCGACTTCCCGGGAGCGTGGCGG-----------------------TCGATGGACT`	`23 bp deleted → frameshift`
188	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG---------------AGCCGGTCGATGGACT`	`15 bp deleted → no frameshift`
179	`CCCTCCGACTTCCCGGGAGCGTGGCGGG----------------AGCCGGTCGATGGACT`	`16 bp deleted → frameshift`
179	`CCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGTCGATGGACT`	`16 bp deleted → frameshift`
170	`CCCTCCGACTTCCCGGGAGCGTGGCGG-----------------AGCCGGTCGATGGACT`	`17 bp deleted → frameshift`
170	`CCCTCCGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGATGGACT`	`17 bp deleted → frameshift`
170	`CCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGTCGATGGACT`	`17 bp deleted → frameshift`
156	`CCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGTCGATGGACT`	`13 bp deleted → frameshift`
147	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG--------------------TCGATGGACT`	`20 bp deleted → frameshift`
147	`CCCTCCGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGATGGACT`	`20 bp deleted → frameshift`
146	`CCCTCCGACTTCCCGG----------------------------------TCGATGGACT`	`34 bp deleted → frameshift`
140	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG---------------------TCGATGGACT`	`21 bp deleted → no frameshift`
140	`CCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGTCGATGGACT`	`21 bp deleted → no frameshift`
133	`CCCTCCGACTTCCCGGGAGCGTGGCGGG----------------------TCGATGGACT`	`22 bp deleted → frameshift`
133	`CCCTCCGACTTCCCGGGAGCGTGGC----------------------CGGTCGATGGACT`	`22 bp deleted → frameshift`
133	`CCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGTCGATGGACT`	`22 bp deleted → frameshift`
133	`CCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGTCGATGGACT`	`22 bp deleted → frameshift`
122	`CCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGATGGACT`	`18 bp deleted → no frameshift`
114	`CCCTCCGACTTCCCGG-------------------------AGGAGCCGGTCGATGGACT`	`25 bp deleted → frameshift`
109	`CCCTCCGACTTCCCGGGAGCGTGG--------------------------TCGATGGACT`	`26 bp deleted → frameshift`
103	`CCCTCCGACTTCCCGGGAGCGTGGCGGGGG---------------------------ACT`	`27 bp deleted → no frameshift`
103	`CCCTCCGACTTCCCGGGAGCGTGGCG---------------------------ATGGACT`	`27 bp deleted → no frameshift`
98	`CCCTCCGACTTCCCGGGAGCGTGGCGGGG----------------------------ACT`	`28 bp deleted → frameshift`
98	`CCCTCCGACTTCCCGGGAGCG----------------------------GTCGATGGACT`	`28 bp deleted → frameshift`
98	`CCCTCCGACTTCCCGG----------------------------AGCCGGTCGATGGACT`	`28 bp deleted → frameshift`
96	`CCCTCCGACTTCCCGGGAGCGTGG---------------------------------ACT`	`33 bp deleted → no frameshift`
94	`CCCTCCGACTTCCCGGGAGCGTGGCGGG-----------------------------ACT`	`29 bp deleted → frameshift`
94	`CCCTCC-----------------------------AGGAGGAGGAGCCGGTCGATGGACT`	`29 bp deleted → frameshift`
89	`CCCTCCGACTTCCCGGGAGCGTGGCGG------------------------------ACT`	`30 bp deleted → no frameshift`
80	`CCCTCCGACTTCCCGGGAGCG--------------------------------ATGGACT`	`32 bp deleted → frameshift`
80	`CCC--------------------------------AGGAGGAGGAGCCGGTCGATGGACT`	`32 bp deleted → frameshift`
76	`CCCTCCGACTTCCCGGG---------------------------------TCGATGGACT`	`33 bp deleted → no frameshift`
76	`CC---------------------------------AGGAGGAGGAGCCGGTCGATGGACT`	`33 bp deleted → no frameshift`
73	`CCCTCCG------------------------------------------GTCGATGGACT`	`42 bp deleted → no frameshift`
70	`CCCTCCGACTTCCCGGGAGCGT-----------------------------CGATGGACT`	`29 bp deleted → frameshift`
69	`CCCTCCGACTTCC-----------------------------------GGTCGATGGACT`	`35 bp deleted → frameshift`
67	`CCCTCCGACTTCCCGGGA----------------------------------------CT`	`40 bp deleted → frameshift`
63	`CCCTCCGA-------------------------------GGAGGAGCCGGTCGATGGACT`	`31 bp deleted → frameshift`
60	`CCCTCCGACTTCCCG--------------------------------------ATGGACT`	`38 bp deleted → frameshift`
54	`CCCTCCGA----------------------------------GGAGCCGGTCGATGGACT`	`34 bp deleted → frameshift`
51	`CCCTCCGACTTCCCGG-----------------------------------------ACT`	`41 bp deleted → frameshift`
50	`CCCTCCGA----------------------------------------------TGGACT`	`46 bp deleted → frameshift`
49	`CCCTCCGACTTCCCGGGA------------------------------------TGGACT`	`36 bp deleted → no frameshift`
49	`CCCTCCGACT--------------------------------------------------`	`50 bp deleted → frameshift`
47	`CCCTCCGA-------------------------------------GCCGGTCGATGGACT`	`37 bp deleted → frameshift`
42	`CCC---------------------------------------------GGTCGATGGACT`	`45 bp deleted → no frameshift`
40	`CCCTCCGACTTC----------------------------------------GATGGACT`	`40 bp deleted → frameshift`
40	`CC----------------------------------------------GGTCGATGGACT`	`46 bp deleted → frameshift`
28	`CCCTC-----------------------------------------------GATGGACT`	`47 bp deleted → frameshift`
18	`CCCT--------------------------------------------------------`	`56 bp deleted → frameshift`

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