← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313785_F | TCGTCGGCAGCGTCCCTTGTCGCCTTAGCTCCTC | 60.1 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313785_R | GTCTCGTGGGCTCGGGTCCTTTCGGCTCTCCATCC | 60.1 | 1.00 |
| mm4_intergenic_Myo18b|Adrbk2_chr5_112897929_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intergenic_Myo18b|Adrbk2_chr5_112897929_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Rgs7_chr1_175454406_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Rgs7_chr1_175454406_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Vrk1_chr12_106034049_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intron_Vrk1_chr12_106034049_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm23558|Galc_chr12_98009523_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm23558|Galc_chr12_98009523_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149356787_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149356787_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868440_F | TCGTCGGCAGCGTCCACTGACGTACTTAACAGTGAGGA | 60.0 | 0.32 |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868440_R | GTCTCGTGGGCTCGGGAACCTCGGTCATGCTGGAA | 60.0 | 0.32 |
| mm4_intergenic_Gab1|Gm27048/Gm4891_chr8_80932319_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gab1|Gm27048/Gm4891_chr8_80932319_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm3_intergenic_Gm10051|Gm15627_chr5_134067821_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_intergenic_Gm10051|Gm15627_chr5_134067821_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Zfp93|Zfp61_chr7_24280829_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Zfp93|Zfp61_chr7_24280829_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm7275|Gm7204_chr16_48104807_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm7275|Gm7204_chr16_48104807_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_exon_Olfr1154_chr2_87903223_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_exon_Olfr1154_chr2_87903223_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm22649|Usp29_chr7_6920914_F | TCGTCGGCAGCGTCGTCTGAGAACACAGGGGCAA | 59.8 | 0.26 |
| mm4_intergenic_Gm22649|Usp29_chr7_6920914_R | GTCTCGTGGGCTCGGGCAGAGTCCCTGTGAAGAGG | 59.7 | 0.26 |
| mm4_intergenic_5033403F01Rik|Gm23972_chr13_39684725_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_5033403F01Rik|Gm23972_chr13_39684725_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intergenic_Rgs6|Gm22149_chr12_82694809_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intergenic_Rgs6|Gm22149_chr12_82694809_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Opn5|Gpr115_chr17_42640816_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Opn5|Gpr115_chr17_42640816_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Tyw1_chr5_130319584_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Tyw1_chr5_130319584_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Cacna1e|Gm9530_chr1_155055774_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Cacna1e|Gm9530_chr1_155055774_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Pitx1|H2afy_chr13_55863084_F | TCGTCGGCAGCGTCTGTGATGCTTGGGTCTGGAC | 59.9 | 0.20 |
| mm4_intergenic_Pitx1|H2afy_chr13_55863084_R | GTCTCGTGGGCTCGGATACTCCCAGTGAGGAGCTG | 58.2 | 0.20 |
| mm3_intron_Gpr64_chrX_160409468_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intron_Gpr64_chrX_160409468_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Atp1a2_chr1_172289261_F | TCGTCGGCAGCGTCCAGTTTCAGGAGTCAGCCCC | 60.3 | 0.19 |
| mm4_intron_Atp1a2_chr1_172289261_R | GTCTCGTGGGCTCGGCTACCAACTGCGTGGAAGGT | 59.9 | 0.19 |
| mm4_intron_Thsd4_chr9_60231543_F | TCGTCGGCAGCGTCACACTGATGCTTAGAAAGGGAAGT | 59.9 | 0.19 |
| mm4_intron_Thsd4_chr9_60231543_R | GTCTCGTGGGCTCGGTCGTCTTGTTACACATCTTTGTTCA | 59.1 | 0.19 |
| mm4_intergenic_Cadm1|Nxpe2_chr9_48290502_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Cadm1|Nxpe2_chr9_48290502_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Dnah6_chr6_73150156_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Dnah6_chr6_73150156_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36857441_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36857441_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Col22a1_chr15_71950279_F | TCGTCGGCAGCGTCACCCAGATCGAAGGAGCAAA | 59.0 | 0.17 |
| mm4_intron_Col22a1_chr15_71950279_R | GTCTCGTGGGCTCGGCTGCCCAGGGAGAAGTCAAC | 60.3 | 0.17 |
| mm4_intergenic_Gm26755|Ssfa2_chr2_79582452_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26755|Ssfa2_chr2_79582452_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Dgkg|Crygs_chr16_22775524_F | TCGTCGGCAGCGTCGGGTTCACTGGCACTATCCC | 60.1 | 0.17 |
| mm4_intergenic_Dgkg|Crygs_chr16_22775524_R | GTCTCGTGGGCTCGGCATCGAGCTGGTTCCTACGA | 59.2 | 0.17 |
| mm3_intron_9430015G10Rik_chr4_156116376_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_9430015G10Rik_chr4_156116376_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47380934_F | TCGTCGGCAGCGTCAGTGGGGATGAGGACTGCTC | 60.9 | 0.15 |
| mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47380934_R | GTCTCGTGGGCTCGGTCTGTGCCCTATCTTGGTCC | 58.7 | 0.15 |
| mm4_intergenic_Ikzf2|Spag16_chr1_69788932_F | TCGTCGGCAGCGTCGACTGACAAAGAGGACCGGG | 60.0 | 0.14 |
| mm4_intergenic_Ikzf2|Spag16_chr1_69788932_R | GTCTCGTGGGCTCGGTCCATTCAATATATGCTTGCTGTGT | 59.1 | 0.14 |
| mm4_intergenic_9230019H11Rik|H60c_chr10_3228915_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_9230019H11Rik|H60c_chr10_3228915_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Fmnl3|Gm22951_chr15_99355851_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Fmnl3|Gm22951_chr15_99355851_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm12789|Gm12799_chr4_101996956_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm12789|Gm12799_chr4_101996956_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Syn3_chr10_86159584_F | TCGTCGGCAGCGTCGAGGGCTCTGAGAGGAGGAA | 60.0 | 0.13 |
| mm4_intron_Syn3_chr10_86159584_R | GTCTCGTGGGCTCGGCTTCTCCTTCCTCGATCGCC | 59.9 | 0.13 |
| mm4_intergenic_Trim29|Pvrl1_chr9_43427196_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Trim29|Pvrl1_chr9_43427196_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Lrrn1|Setmar_chr6_107623201_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Lrrn1|Setmar_chr6_107623201_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6531|Olfr371_chr8_85174380_F | TCGTCGGCAGCGTCGCACACTGGGACAAAGAGGA | 59.8 | 0.12 |
| mm4_intergenic_Gm6531|Olfr371_chr8_85174380_R | GTCTCGTGGGCTCGGTGAGAAAATGATAATGAAGGCCACT | 58.8 | 0.12 |
| mm4_intergenic_Fau-ps1|Macrod2_chr2_141944759_F | TCGTCGGCAGCGTCTGAGAGTTTTCTTATGGCAGCA | 57.9 | 0.12 |
| mm4_intergenic_Fau-ps1|Macrod2_chr2_141944759_R | GTCTCGTGGGCTCGGTCTTGCATTCGCTGACCCTT | 59.9 | 0.12 |
| mm4_intron_Eps8l2_chr7_141343473_F | TCGTCGGCAGCGTCGCCTTTCTCCTGGTCACCTC | 60.0 | 0.11 |
| mm4_intron_Eps8l2_chr7_141343473_R | GTCTCGTGGGCTCGGAGCTCAGTTTATGGCCAGAAA | 57.5 | 0.11 |
| mm3_intron_Lonp2_chr8_86705363_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intron_Lonp2_chr8_86705363_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Xrcc4|Gm4117_chr13_89856412_F | TCGTCGGCAGCGTCCAACAGTGCTTGTGCCTCAC | 59.9 | 0.11 |
| mm4_intergenic_Xrcc4|Gm4117_chr13_89856412_R | GTCTCGTGGGCTCGGGACAACTTTTGGGACGTGCC | 59.9 | 0.11 |
| mm4_intron_Sept8_chr11_53525599_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Sept8_chr11_53525599_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm24535|Gm14563_chrX_22054751_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm24535|Gm14563_chrX_22054751_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm22536|Gm6632_chr5_58462721_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm22536|Gm6632_chr5_58462721_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23848|Trps1_chr15_50581049_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23848|Trps1_chr15_50581049_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Veph1|Shox2_chr3_66423976_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Veph1|Shox2_chr3_66423976_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11999|Ikzf1_chr11_11658649_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11999|Ikzf1_chr11_11658649_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm25258|Hao1_chr2_133881233_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm25258|Hao1_chr2_133881233_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26614|Zcwpw2_chr9_117875061_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm26614|Zcwpw2_chr9_117875061_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Dync1i1_chr6_5893610_F | TCGTCGGCAGCGTCAGGGACTTCATGTTTGAGCCA | 59.5 | 0.07 |
| mm4_intron_Dync1i1_chr6_5893610_R | GTCTCGTGGGCTCGGTCGCAGTCTGATTCTGAACGA | 59.4 | 0.07 |
| mm4_intergenic_Gm23735|Fndc3a_chr14_72495185_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23735|Fndc3a_chr14_72495185_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Pacsin2|Ttll1_chr15_83470603_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Pacsin2|Ttll1_chr15_83470603_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Sh3rf3|AC153944.1_chr10_58891248_F | TCGTCGGCAGCGTCGCAGATCCATGTCCCCTGAG | 59.8 | 0.07 |
| mm4_intergenic_Sh3rf3|AC153944.1_chr10_58891248_R | GTCTCGTGGGCTCGGCAACTGCTTTGCCAACCCC | 59.9 | 0.07 |
| mm4_intergenic_Gm26029|Gm22225_chrX_129195807_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm26029|Gm22225_chrX_129195807_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm6564|AC158942.1_chr5_23038889_F | TCGTCGGCAGCGTCTCCTGGGAGGACACTAAGGG | 59.9 | 0.07 |
| mm3_intergenic_Gm6564|AC158942.1_chr5_23038889_R | GTCTCGTGGGCTCGGAGGTGAAAGCTCCAAGGTGG | 59.8 | 0.07 |
| mm4_intron_Cuedc1_chr11_88165499_F | TCGTCGGCAGCGTCTAAAGATGAGGGGCGCTTCG | 60.1 | 0.07 |
| mm4_intron_Cuedc1_chr11_88165499_R | GTCTCGTGGGCTCGGCATGAAAGAAAGGAGGCGCG | 59.9 | 0.07 |
| mm4_intergenic_Gm26360|Tmem108_chr9_103605069_F | TCGTCGGCAGCGTCGGAACTGGATGCAGAGGCTG | 60.7 | 0.07 |
| mm4_intergenic_Gm26360|Tmem108_chr9_103605069_R | GTCTCGTGGGCTCGGAGAGACCTGAGTCACTGGCT | 59.8 | 0.07 |
| mm4_intron_Camkk1_chr11_73036243_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Camkk1_chr11_73036243_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_C330008G21Rik|Ptprt_chr2_162626694_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_C330008G21Rik|Ptprt_chr2_162626694_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm25702|Gm5473_chr15_63579692_F | TCGTCGGCAGCGTCAGATTCCATCTGGATAGAATGGTGA | 59.1 | 0.06 |
| mm4_intergenic_Gm25702|Gm5473_chr15_63579692_R | GTCTCGTGGGCTCGGGCCCAAGTGGAAGAAAGGGA | 59.8 | 0.06 |
| mm4_intron_Col6a1_chr10_76712680_F | TCGTCGGCAGCGTCTGCTTCAGCCTGTTGATGGT | 59.8 | 0.06 |
| mm4_intron_Col6a1_chr10_76712680_R | GTCTCGTGGGCTCGGGTTTCCCTTCCCAGTGAGCA | 59.8 | 0.06 |
| mm4_intergenic_Tmem200c|Epb4.1l3_chr17_68931176_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tmem200c|Epb4.1l3_chr17_68931176_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ank3_chr10_70011786_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ank3_chr10_70011786_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Pja1|Gm14816_chrX_99527408_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Pja1|Gm14816_chrX_99527408_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm15233|Arhgap6_chrX_168794734_F | TCGTCGGCAGCGTCCCAAGATTTGGCAGCTGAGC | 59.8 | 0.06 |
| mm4_intergenic_Gm15233|Arhgap6_chrX_168794734_R | GTCTCGTGGGCTCGGAAAGTTAACTGGCCTCCGGG | 59.9 | 0.06 |
| mm4_intergenic_Pde7b|Ahi1_chr10_20908803_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Pde7b|Ahi1_chr10_20908803_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Kcna6|Kcna6/Gm26673_chr6_126732832_F | TCGTCGGCAGCGTCTTAAGGCAGCAGTTCCAGCA | 59.8 | 0.05 |
| mm4_intergenic_Kcna6|Kcna6/Gm26673_chr6_126732832_R | GTCTCGTGGGCTCGGGCTTCTGTGATGCAGGCTTG | 59.8 | 0.05 |
| mm3_intergenic_Gm12700|Cachd1_chr4_100749553_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm12700|Cachd1_chr4_100749553_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_1700001G01Rik|Gm26235_chr18_17908665_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_1700001G01Rik|Gm26235_chr18_17908665_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15397|Gm6407_chr10_73575048_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15397|Gm6407_chr10_73575048_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_AC102738.1|St6galnac3_chr3_153353487_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_AC102738.1|St6galnac3_chr3_153353487_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21943|Gm21724_chrY_58021491_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21943|Gm21724_chrY_58021491_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21918|Gm21832_chrY_61037318_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21918|Gm21832_chrY_61037318_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21916|Gm21756_chrY_33180599_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21916|Gm21756_chrY_33180599_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21914|Gm21852_chrY_40518214_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21914|Gm21852_chrY_40518214_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21861|Gm21758_chrY_58888303_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21861|Gm21758_chrY_58888303_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21842|Gm21744_chrY_32322058_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21842|Gm21744_chrY_32322058_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21822|Gm21763_chrY_18911680_F | TCGTCGGCAGCGTCGCACAGGCAATCCAAGGTTC | 59.7 | 0.05 |
| mm4_intergenic_Gm21822|Gm21763_chrY_18911680_R | GTCTCGTGGGCTCGGTCTGGGGTTATACATGGTGGT | 58.1 | 0.05 |
| mm4_intergenic_Gm21805|Gm21241_chrY_42873097_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21805|Gm21241_chrY_42873097_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21803|Gm20837_chrY_90336261_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21803|Gm20837_chrY_90336261_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21780|Gm21737_chrY_31307400_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21780|Gm21737_chrY_31307400_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21779|Gm21247_chrY_45891024_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21779|Gm21247_chrY_45891024_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21767|Gm20822_chrY_14199410_F | TCGTCGGCAGCGTCGCACAGGCAATCCAAGGTTC | 59.7 | 0.05 |
| mm4_intergenic_Gm21767|Gm20822_chrY_14199410_R | GTCTCGTGGGCTCGGTCTGGGGTTATACATGGTGGT | 58.1 | 0.05 |
| mm4_intergenic_Gm21758|Gm21843_chrY_59695244_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21758|Gm21843_chrY_59695244_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21751|Gm21271_chrY_48246923_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21751|Gm21271_chrY_48246923_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21745|Gm21184_chrY_34226362_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21745|Gm21184_chrY_34226362_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21739|Gm21856_chrY_44410439_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21739|Gm21856_chrY_44410439_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21739|Gm21856_chrY_44321891_F | TCGTCGGCAGCGTCACTTCTCTCATCGTCTGCAGT | 58.8 | 0.05 |
| mm4_intergenic_Gm21739|Gm21856_chrY_44321891_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21724|Gm21405_chrY_58508785_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21724|Gm21405_chrY_58508785_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21679|Gm21529_chrY_30812193_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21679|Gm21529_chrY_30812193_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21679|Gm21529_chrY_29749771_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21679|Gm21529_chrY_29749771_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21247|Gm21256_chrY_47385998_F | TCGTCGGCAGCGTCAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21247|Gm21256_chrY_47385998_R | GTCTCGTGGGCTCGGCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21247|Gm21256_chrY_46832972_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21247|Gm21256_chrY_46832972_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21170|Gm21751_chrY_47752955_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21170|Gm21751_chrY_47752955_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm21094|Gm21588_chrY_32685432_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm21094|Gm21588_chrY_32685432_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_Gm20901|Gm21865_chrY_39389564_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20901|Gm21865_chrY_39389564_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20901|Gm21865_chrY_38960203_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20901|Gm21865_chrY_38960203_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20838|Gm21626_chrY_33608101_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20838|Gm21626_chrY_33608101_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20810|Gm21853_chrY_29134561_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20810|Gm21853_chrY_29134561_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm20794|Gm21918_chrY_60543337_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_Gm20794|Gm21918_chrY_60543337_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intergenic_1700040F15Rik|Gm21562_chrY_31708385_F | TCGTCGGCAGCGTCCTTCTCTCATCGTCTGCGGT | 59.5 | 0.05 |
| mm4_intergenic_1700040F15Rik|Gm21562_chrY_31708385_R | GTCTCGTGGGCTCGGAAGGATCACCATGCTTCCCA | 58.9 | 0.05 |
| mm4_intron_AI481877_chr4_59106901_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_AI481877_chr4_59106901_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Sspn_chr6_145963250_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Sspn_chr6_145963250_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm11923|Gm11925_chr4_29800297_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm11923|Gm11925_chr4_29800297_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Zkscan2|Gm23788_chr7_123691921_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Zkscan2|Gm23788_chr7_123691921_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Cwf19l1_chr19_44127036_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Cwf19l1_chr19_44127036_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kalrn_chr16_34419550_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kalrn_chr16_34419550_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tgm7_chr2_121103534_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tgm7_chr2_121103534_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Grxcr1|Gm23605_chr5_68206915_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Grxcr1|Gm23605_chr5_68206915_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Uncx|Micall2_chr5_139638368_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Uncx|Micall2_chr5_139638368_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Auts2_chr5_131707749_F | TCGTCGGCAGCGTCGTTTTGGCAAGCGGTCTGG | 60.0 | 0.04 |
| mm3_intron_Auts2_chr5_131707749_R | GTCTCGTGGGCTCGGTCTGGAGCCTGTTCAAACTCA | 59.2 | 0.04 |
| mm4_intron_Slc9a2_chr1_40734902_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Slc9a2_chr1_40734902_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21966522_F | TCGTCGGCAGCGTCAGCTAGGCTCATCTGGGTCA | 60.0 | 0.03 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21966522_R | GTCTCGTGGGCTCGGGTTGGGGAGAGTGACCATGG | 60.0 | 0.03 |
| mm4_intergenic_Klf9|Mir1192_chr19_23143039_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Klf9|Mir1192_chr19_23143039_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24598|Gm14676_chrX_64666668_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24598|Gm14676_chrX_64666668_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_30476726_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_30476726_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sorbs3_chr14_70195852_F | TCGTCGGCAGCGTCTCTTTCATGGGATCGCACCA | 59.3 | 0.03 |
| mm4_intron_Sorbs3_chr14_70195852_R | GTCTCGTGGGCTCGGCTGGACCTTGGAAGACCCAC | 59.9 | 0.03 |
| mm3_intergenic_Nek7|Lhx9_chr1_138771300_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Nek7|Lhx9_chr1_138771300_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Adipor1_chr1_134427507_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Adipor1_chr1_134427507_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Pcx_chr19_4604260_F | TCGTCGGCAGCGTCTTCACCTAGACTTGCTGCGG | 60.0 | 0.02 |
| mm4_intron_Pcx_chr19_4604260_R | GTCTCGTGGGCTCGGGTGGCCGCAACCCAGAATAT | 60.7 | 0.02 |
| mm4_intron_Ago4_chr4_126495219_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ago4_chr4_126495219_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ptch1|Ercc6l2_chr13_63688100_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ptch1|Ercc6l2_chr13_63688100_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Zfp442|Gm14127_chr2_150458591_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Zfp442|Gm14127_chr2_150458591_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14139|Zfp937_chr2_150210741_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14139|Zfp937_chr2_150210741_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26651|Diras2_chr13_52189153_F | TCGTCGGCAGCGTCCCAGCCCTACGTGTTCTGAG | 60.1 | 0.01 |
| mm4_intergenic_Gm26651|Diras2_chr13_52189153_R | GTCTCGTGGGCTCGGGCTGATCTGGGTCAGTGGTG | 60.3 | 0.01 |
| mm4_intergenic_Rarb|Thrb_chr14_16797504_F | TCGTCGGCAGCGTCTTCTGCTCTGGTCTGTCTGC | 59.6 | 0.01 |
| mm4_intergenic_Rarb|Thrb_chr14_16797504_R | GTCTCGTGGGCTCGGGGGACTGGGAAATCACGACA | 59.6 | 0.01 |
| mm4_intron_Shank3_chr15_89544655_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Shank3_chr15_89544655_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14005|Gm14010_chr2_128398659_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14005|Gm14010_chr2_128398659_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm25423|Gm26054_chr7_116716283_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm25423|Gm26054_chr7_116716283_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Neurog1|Cxcl14_chr13_56259422_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Neurog1|Cxcl14_chr13_56259422_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A730085K08Rik|9530059O14Rik_chr9_122499548_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A730085K08Rik|9530059O14Rik_chr9_122499548_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Itih5_chr2_10225553_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Itih5_chr2_10225553_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22708|Zbtb46_chr2_181435692_F | TCGTCGGCAGCGTCCAGGACCAAGGGAGCAGAAA | 59.5 | 0.00 |
| mm4_intergenic_Gm22708|Zbtb46_chr2_181435692_R | GTCTCGTGGGCTCGGGAGATTAAGCAGGGCAGCCT | 59.8 | 0.00 |
| mm4_intergenic_Gm22506|RP23-448C3.1_chr19_22335062_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22506|RP23-448C3.1_chr19_22335062_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcna4|Gm9864_chr2_107383526_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcna4|Gm9864_chr2_107383526_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14215|Gm14216_chr2_154714027_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14215|Gm14216_chr2_154714027_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cntn6_chr6_104640396_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cntn6_chr6_104640396_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Map3k9_chr12_81732199_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Map3k9_chr12_81732199_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_1500017E21Rik_chr19_36648487_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_1500017E21Rik_chr19_36648487_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25602|Tbc1d32_chr10_54945126_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25602|Tbc1d32_chr10_54945126_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ercc6_chr14_32536440_F | TCGTCGGCAGCGTCTGAGACCTCCTGTGTGCCTA | 59.8 | 0.00 |
| mm4_intron_Ercc6_chr14_32536440_R | GTCTCGTGGGCTCGGTCCTTTGCCTTAGAGGAAGCTG | 60.0 | 0.00 |
| mm4_intron_4933436C20Rik_chr8_92335678_F | TCGTCGGCAGCGTCAGTTACACTCCACGCTCTGC | 60.0 | 0.00 |
| mm4_intron_4933436C20Rik_chr8_92335678_R | GTCTCGTGGGCTCGGGCAGCGCAACCATCATTGAT | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313785 |
CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTC CCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGAC |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868440 |
CACTGACGTACTTAACAGTGAGGAAAAACATGGGGTTTGGAGCTCACTTGGGGAAGCATTGGTGCTGTTCCAGCATGACC GAGGTTC |
| mm4_intergenic_Gm22649|Usp29_chr7_6920914 |
GTCTGAGAACACAGGGGCAAGCTCCTCACCCAGTAAGCTCCTCACACCAGCCTGGATTTCTCTTCCCCTCTTCACAGGGA CTCTGC |
| mm4_intergenic_Pitx1|H2afy_chr13_55863084 |
TGTGATGCTTGGGTCTGGACCCCAGAGCCTCACACATCTAGAAGAACGACTTTCCCATTAAGCTCCTACCCCCAGCTCTG CCCAGGCCCAGCTCCTCACTGGGAGTAT |
| mm4_intron_Atp1a2_chr1_172289261 |
CAGTTTCAGGAGTCAGCCCCTTCCCTGCACTATGCTGGGGTGAGGAGCTTTCTTGGGAGGCTAGCGTCTCACCTTCCACG CAGTTGGTAG |
| mm4_intron_Thsd4_chr9_60231543 |
ACACTGATGCTTAGAAAGGGAAGTATGTTGCCTATAATAAATAGTATTACTATAAATATACAAAAATAAAGACTAAAACT AAATAACCCTTTTAAGCTCCAAATCCCAAGGATTTGAACAAAGATGTGTAACAAGACGA |
| mm4_intron_Col22a1_chr15_71950279 |
ACCCAGATCGAAGGAGCAAATGAGAGCTAAGAAATTATATACCTTTCCAAGTAAGCTGTGAGTCCCAGGCACCAACTAGG CCTTTTGAGTTCTAATCAAGGGATCTTGGGTCTGGAAATGTTGACTTCTCCCTGGGCAG |
| mm4_intergenic_Dgkg|Crygs_chr16_22775524 |
GGGTTCACTGGCACTATCCCATAGCATCCCCTTAGGTTGTATGGGGGAATTAAGAGATCTGCCTGAGTGCCACAGTGGGC TCACCTAGCTAGCTCCAAATCCCAAGTCGTAGGAACCAGCTCGATG |
| mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47380934 |
AGTGGGGATGAGGACTGCTCCTTGCTTTATGGTTGCCCTGGAGTTATGAGCTTACAGGGACTCTTTTTCAAATTACTTTC TAGCTCATGAGCCAAAGAGTTATGCACTTTTCAGTGCTTAGGACCAAGATAGGGCACAGA |
| mm4_intergenic_Ikzf2|Spag16_chr1_69788932 |
GACTGACAAAGAGGACCGGGAGAACAATGCTTAAGCATTGCTCTGGGGGTTGGGTGGTTCATTTCCCTGGGGTCAGGAGC TAAATGGGGTAACACAGCAAGCATATATTGAATGGA |
| mm4_intron_Syn3_chr10_86159584 |
GAGGGCTCTGAGAGGAGGAACATGGACCCAGGAAGCTCCTATTCCTAGTCAGCAGGAACTGCTCTGCTGTACTGGAACCA GCAGGCGATCGAGGAAGGAGAAG |
| mm4_intergenic_Gm6531|Olfr371_chr8_85174380 |
GCACACTGGGACAAAGAGGAAGCACACTTTCTCAGCAGAGAATCTCGATGTTCTGCCAAGTAAGCTGCTTACCCAAGGAA GTGGCCTTCATTATCATTTTCTCA |
| mm4_intergenic_Fau-ps1|Macrod2_chr2_141944759 |
TGAGAGTTTTCTTATGGCAGCATAACTGGGAGCATAATTAACCATGAAGACACAGTGTATTTCCATTTTAGCTTCTAATC CCAGGAGATACCATGTTTTAGAGCTATGAGTAATTAAAGGGTCAGCGAATGCAAGA |
| mm4_intron_Eps8l2_chr7_141343473 |
GCCTTTCTCCTGGTCACCTCTAATTTGCTGAGAATTCAAGAACTATGTTTTAATGGCTTTCCTGGGACTGGGAGCTAGCT GGGTTTCTGGCCATAAACTGAGCT |
| mm4_intergenic_Xrcc4|Gm4117_chr13_89856412 |
CAACAGTGCTTGTGCCTCACTAGGTACCTGGGATTACTGATTTCTGTATTTCCTCCAAGGATGCTCCTACTCTCAGGAGT GTAACATTAATGTGGATATGGCACGTCCCAAAAGTTGTC |
| mm4_intron_Dync1i1_chr6_5893610 |
AGGGACTTCATGTTTGAGCCAATGGGAAAACATGAAGTTAACACTAGGGGAAATGACTTAGCCCCCAATAAAGCTGCTAA TGCCAGGATAAAAGCAAACAAATCTGTTAATGATCTTCGTTCAGAATCAGACTGCGA |
| mm4_intergenic_Sh3rf3|AC153944.1_chr10_58891248 |
GCAGATCCATGTCCCCTGAGGCTTGGGGAAATGAGTGCTGACAAGCGAGGAGTCCCAGCCACTCCGAGGCTGGGATCAGG AACTGACTTGGAGATTTCACAACCACCATCCAGGTGCAAGGGGGGTTGGCAAAGCAGTTG |
| mm3_intergenic_Gm6564|AC158942.1_chr5_23038889 |
TCCTGGGAGGACACTAAGGGTGTCCTTTTAGACACAGTCAGCATAGGAGGGCCTGCCAGGAGGAGACCCATAGCCTGTGA CCAGGAGCTTACTCAGACTACCCACCTTGGAGCTTTCACCT |
| mm4_intron_Cuedc1_chr11_88165499 |
TAAAGATGAGGGGCGCTTCGGTGGTGACAGGGGAAAGTGTCCCGTGTAAGTTCATAAGCCCAGGTCATCTGCGCGCCTCC TTTCTTTCATG |
| mm4_intergenic_Gm26360|Tmem108_chr9_103605069 |
GGAACTGGATGCAGAGGCTGCAGAGGCCATGGTCTTGGGTTTAGAAGCTTCCTGGGTTCTGGCCTGAAGAATCTCTTATA TCTACCACACATTTGTATCTATCATACAAAGCCAGTGACTCAGGTCTCT |
| mm4_intergenic_Gm25702|Gm5473_chr15_63579692 |
AGATTCCATCTGGATAGAATGGTGAAATTTTTTAATAAAATGTCTGGGATAAGTAGCTTCCTAGGTCCCTCTATAGATGG TGGCCATAAGAATACAAGCTCCTTCCCTTTCTTCCACTTGGGC |
| mm4_intron_Col6a1_chr10_76712680 |
TGCTTCAGCCTGTTGATGGTTACTTGAAACATTAGGGAGAAAGGCCAGGGATCAGCAGCTTCCTAGGAGGCCTGACCCCA GGGCACTGCTCACTGGGAAGGGAAAC |
| mm4_intergenic_Gm15233|Arhgap6_chrX_168794734 | CCAAGATTTGGCAGCTGAGCCCTGTGCTCAGGAGCTTTCTGGGGCGACCCCGGAGGCCAGTTAACTTT |
| mm4_intergenic_Kcna6|Kcna6/Gm26673_chr6_126732832 |
TTAAGGCAGCAGTTCCAGCAGACGGCTCAGGGAGCCTGGCATCTCCTGGGAATGGGATCTTTCTTGGCATTTACATGGAG ATGCAAAGTGAGAGAAATAGCACTGCCAGCCAAGCCTGCATCACAGAAGC |
| mm4_intergenic_Gm21943|Gm21724_chrY_58021491 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21861|Gm21758_chrY_58888303 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21822|Gm21763_chrY_18911680 |
GCACAGGCAATCCAAGGTTCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATG CTCCTAATCCTAGGGATCATTTAGAGGTCTCATAAATACCACCATGTATAACCCCAGA |
| mm4_intergenic_Gm21805|Gm21241_chrY_42873097 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21803|Gm20837_chrY_90336261 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21780|Gm21737_chrY_31307400 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21779|Gm21247_chrY_45891024 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21767|Gm20822_chrY_14199410 |
GCACAGGCAATCCAAGGTTCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATG CTCCTAATCCTAGGGATCATTTAGAGGTCTCATAAATACCACCATGTATAACCCCAGA |
| mm4_intergenic_Gm21758|Gm21843_chrY_59695244 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21751|Gm21271_chrY_48246923 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21745|Gm21184_chrY_34226362 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21739|Gm21856_chrY_44321891 |
ACTTCTCTCATCGTCTGCAGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGG TAGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21724|Gm21405_chrY_58508785 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21679|Gm21529_chrY_30812193 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21247|Gm21256_chrY_47385998 |
AAGGATCACCATGCTTCCCATTTTATCTCTCCAGTCTATATATCCACTCCCAACTCTACCATCTATGCTCCTAATCCTAG GGATCATTTAGAGGTCTCATAAATATCACCATGTATAACCGCAGACGATGAGAGAAG |
| mm4_intergenic_Gm21247|Gm21256_chrY_46832972 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21170|Gm21751_chrY_47752955 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm21094|Gm21588_chrY_32685432 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_Gm20794|Gm21918_chrY_60543337 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm4_intergenic_1700040F15Rik|Gm21562_chrY_31708385 |
CTTCTCTCATCGTCTGCGGTTATACATGGTGATATTTATGAGACCTCTAAATGATCCCTAGGATTAGGAGCATAGATGGT AGAGTTGGGAGTGGATATATAGACTGGAGAGATAAAATGGGAAGCATGGTGATCCTT |
| mm3_intron_Auts2_chr5_131707749 |
GTTTTGGCAAGCGGTCTGGTGTTCCCCTACCAAGCTCCTAATCCCAGCAGATCAGTTAAAATAAATGAAAGAGAGAATCA AAAGGCACTTAAAAAATTCAACTGTTCTCCAAATGAGTTTGAACAGGCTCCAGA |
| mm4_intergenic_Gm5633|Gm22908_chr12_21966522 |
AGCTAGGCTCATCTGGGTCAACCCACTCCAGTCACCCAGGAAGCTCCTACTCACAGCCTGCAGCCTGTTCAGACACAAAC ATCCCACCACTTTTTGGGGGCGAATTTCTCAGACTCCATGGTCACTCTCCCCAAC |
| mm4_intron_Sorbs3_chr14_70195852 |
TCTTTCATGGGATCGCACCACCCAGGAAGCCCCCAAACCCAGGCCACACGTCTCTAGACTCCAACCAATGAACCTACACC CCTCACTCACCTTTGGGTGGGTCTTCCAAGGTCCAG |
| mm4_intron_Pcx_chr19_4604260 |
TTCACCTAGACTTGCTGCGGGTAGCCTGGCCTTAGTAGCTTCCTGGGATCTTTGAGACTGTAGAGTGTAGGGGTGGAGGT ATATTCTGGGTTGCGGCCAC |
| mm4_intergenic_Gm26651|Diras2_chr13_52189153 |
CCAGCCCTACGTGTTCTGAGTTCTAGCCTCCCAGAAGCCTCCAAATCCCAGGAACAAAAGACACCACTGACCCAGATCAG C |
| mm4_intergenic_Rarb|Thrb_chr14_16797504 |
TTCTGCTCTGGTCTGTCTGCCCCTACCCCTTCTCCATGCCCCTACTTCCTTTTTGCCCAAATAACCTCCTTATACCAGGT GTATTGCATGTCGTGATTTCCCAGTCCC |
| mm4_intergenic_Gm22708|Zbtb46_chr2_181435692 |
CAGGACCAAGGGAGCAGAAAGTTCTGTGACTCACAAATTCAAGCTGTGGCATTTGTGACAGTCATACGACACTTCTACCA AGTAGCCACCTAATACCAGGGAAGCAAAGGCTGCCCTGCTTAATCTC |
| mm4_intron_Ercc6_chr14_32536440 |
TGAGACCTCCTGTGTGCCTAGGACGTCCTAGGATTAGGATGGTACTTGGTAGACCAGATGCCTAAGTCTACTTGACCAGG AACAGCTTCCTCTAAGGCAAAGGA |
| mm4_intron_4933436C20Rik_chr8_92335678 |
AGTTACACTCCACGCTCTGCAGCTGTGAGAAAGTACCTGGTATTATGAGCTTAGCAGGTCCTCCAATTAGGGCCAAGCTG AAAGCCGTGCACGGCCTTTCGGAGCCCATCAGTATCAATGATGGTTGCGCTGC |