CRISPOR

DNA break repair outcome predictions

Guide: ATCCCAGGGAGCCTGGGATT AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGA`	(Wild-type sequence)
447	`CTCAGCCAAGATCCCAGGGAGC--------------TTACTTGGGGGTTTTCCCCCTCCC`	`14 bp deleted → frameshift`
370	`CTCAGCCAAGATCCCAGGGAGCCTGGGA------GCTTACTTGGGGGTTTTCCCCCTCCC`	`6 bp deleted → no frameshift`
284	`CTCAGCCAAGATCCCAGGGAGCCTGGG------------------GGTTTTCCCCCTCCC`	`18 bp deleted → no frameshift`
282	`CTCAGCCAAGATCCCAGGGAGCCTGG-------AGCTTACTTGGGGGTTTTCCCCCTCCC`	`7 bp deleted → frameshift`
235	`CTCAGCCAAGATCCCAGG---------------AGCTTACTTGGGGGTTTTCCCCCTCCC`	`15 bp deleted → no frameshift`
232	`CTCAGCCAAGATCCCAGGGAGCCTGGG-------------------GTTTTCCCCCTCCC`	`19 bp deleted → frameshift`
220	`CTCAGCCAAGATCCCAGGGAGCCTGGG--------------------TTTTCCCCCTCCC`	`20 bp deleted → frameshift`
173	`CTCAGCCAAGATCCCAGGGAG-----------GAGCTTACTTGGGGGTTTTCCCCCTCCC`	`11 bp deleted → frameshift`
170	`CTCAGCCAAGATCCCAGGGAGCCTGGG-----------------GGGTTTTCCCCCTCCC`	`17 bp deleted → frameshift`
163	`CTCAGCCAAGATCCCAGGG--------------------------GGTTTTCCCCCTCCC`	`26 bp deleted → frameshift`
156	`CTCAGCCAAGATCCCAGGGAGCCT-------------TACTTGGGGGTTTTCCCCCTCCC`	`13 bp deleted → frameshift`
155	`CTCAGCCAAGATCCCAGGG---------------------------GTTTTCCCCCTCCC`	`27 bp deleted → no frameshift`
148	`CTCAGCCAAGATCCCAGGG----------------------------TTTTCCCCCTCCC`	`28 bp deleted → frameshift`
140	`CTCAGCCAAGATCCCAGGGAGCCTGG---------------------TTTTCCCCCTCCC`	`21 bp deleted → no frameshift`
134	`CTCAGCCAAGATCCCAGGGAGCCTGGGATT--------ACTTGGGGGTTTTCCCCCTCCC`	`8 bp deleted → frameshift`
128	`CTCAGCCAAGATCCCAGGGAGCCT-----------------TGGGGGTTTTCCCCCTCCC`	`17 bp deleted → frameshift`
122	`CTCAGCCAAGATCCCAG------------------CTTACTTGGGGGTTTTCCCCCTCCC`	`18 bp deleted → no frameshift`
114	`CTCAGCCAAGATCCCAGGG-------------------------GGGTTTTCCCCCTCCC`	`25 bp deleted → frameshift`
111	`CTCAGC------------------------------TTACTTGGGGGTTTTCCCCCTCCC`	`30 bp deleted → no frameshift`
109	`CTCAGCCAAGATCCCAGGGAGCCTGGGATT------------GGGGGTTTTCCCCCTCCC`	`12 bp deleted → no frameshift`
99	`CTCAGCCAAG----------------------GAGCTTACTTGGGGGTTTTCCCCCTCCC`	`22 bp deleted → frameshift`
99	`CTCAGCCAAGATCCC---------------------------------------CCTCCC`	`39 bp deleted → no frameshift`
96	`CTCAGCCAAGATCCCAGGGAGCCT---------------------------------CCC`	`33 bp deleted → no frameshift`
95	`CTCAGCCAAGA-----------------------GCTTACTTGGGGGTTTTCCCCCTCCC`	`23 bp deleted → frameshift`
94	`CTCAGCCAAGATCCCAGG-----------------------------TTTTCCCCCTCCC`	`29 bp deleted → frameshift`
89	`CTCAGCCAAGATCCCAGGGAGCC------------------------------CCCTCCC`	`30 bp deleted → no frameshift`
86	`CTCAGCCAAG-------------------------CTTACTTGGGGGTTTTCCCCCTCCC`	`25 bp deleted → frameshift`
84	`CTCAGCCAAGATCCCAGGGAGCC-------------------------------CCTCCC`	`31 bp deleted → frameshift`
81	`CTCAGCCAAGATCCC----------------------------------------CTCCC`	`40 bp deleted → frameshift`
80	`CTCAGCCAAGATCCCAGGGAGCC--------------------------------CTCCC`	`32 bp deleted → frameshift`
77	`CTCAG---------------------------GAGCTTACTTGGGGGTTTTCCCCCTCCC`	`27 bp deleted → no frameshift`
77	`CTCAGCCAAGATCCCAGGGAGCCTGGGATT-------------------TTCCCCCTCCC`	`19 bp deleted → frameshift`
77	`CTCAGCCAAGATCCC-----------------------------------------TCCC`	`41 bp deleted → frameshift`
73	`CTCAGCCAAGATCCCAGGGAGCCTGGGATT--------------------TCCCCCTCCC`	`20 bp deleted → frameshift`
73	`CTCAGCCAAGATCCC---------------------------------------------`	`45 bp deleted → no frameshift`
70	`CTCAGCCAAGATCCCAGGGAGCCTGGGATT---------------------CCCCCTCCC`	`21 bp deleted → no frameshift`
66	`CTCAGCCAAGATCCCAGGGAGCC------------------------------------C`	`36 bp deleted → no frameshift`
62	`CTCAGCCAAGATCCCAGGGAGCC-------------------------------------`	`37 bp deleted → frameshift`
60	`CTCAGCCAAGATCCC--------------------------------------CCCTCCC`	`38 bp deleted → frameshift`
52	`CT-----------------------------------TACTTGGGGGTTTTCCCCCTCCC`	`35 bp deleted → frameshift`
48	`CTCAGCCAAGATCC------------------------------------------TCCC`	`42 bp deleted → no frameshift`
44	`CTCAGCCAAGATCCC--------------------------------------------C`	`44 bp deleted → frameshift`
42	`CT---------------------------------------TGGGGGTTTTCCCCCTCCC`	`39 bp deleted → no frameshift`
40	`CTCAGCCAAGATCC----------------------------------------------`	`46 bp deleted → frameshift`
40	`CTCAGCC----------------------------------------------CCCTCCC`	`46 bp deleted → frameshift`
38	`CTCAGCC-----------------------------------------------CCTCCC`	`47 bp deleted → frameshift`
36	`CTCAGCC------------------------------------------------CTCCC`	`48 bp deleted → no frameshift`
34	`CTCAGCC-------------------------------------------------TCCC`	`49 bp deleted → frameshift`
32	`CTC-------------------------------------------------------CC`	`55 bp deleted → frameshift`
29	`CTCAGCC----------------------------------------------------C`	`52 bp deleted → frameshift`
28	`CTCAGCC-----------------------------------------------------`	`53 bp deleted → frameshift`
25	`CTC-------------------------------------------------CCCCTCCC`	`49 bp deleted → frameshift`

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