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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| CCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCC | (Wild-type sequence) | |
| 815 | CTCCATCCCCGGACTCTCCAGAGGGGGGGA------AAACCCCCAAGTAAGCTCCTAATC | 6 bp deleted → no frameshift |
| 705 | CTCCATCCCCGGACTCTCCAGAGGGGGG-------AAAACCCCCAAGTAAGCTCCTAATC | 7 bp deleted → frameshift |
| 670 | CTCCATCCCCGGACTCTCCAGAGGGGG--------AAAACCCCCAAGTAAGCTCCTAATC | 8 bp deleted → frameshift |
| 623 | CTCCATCCCCGGACTCTCCAGAGGGGGGG-----GAAAACCCCCAAGTAAGCTCCTAATC | 5 bp deleted → frameshift |
| 510 | CTCCATCCCCGGACTCTCCAGAGGGG---------AAAACCCCCAAGTAAGCTCCTAATC | 9 bp deleted → no frameshift |
| 491 | CTCCATCCCCGGACTCTCCAGAGGGGGGG----GGAAAACCCCCAAGTAAGCTCCTAATC | 4 bp deleted → frameshift |
| 364 | CTCCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCCTAATC | 10 bp deleted → frameshift |
| 344 | CTCCATCCCCGGACTCTCCAGAGGGGGGG---GGGAAAACCCCCAAGTAAGCTCCTAATC | 3 bp deleted → no frameshift |
| 230 | CTCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCCTAATC | 11 bp deleted → frameshift |
| 158 | CTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCCTAATC | 23 bp deleted → frameshift |
| 153 | CTCCATCCCC---------------------------------CAAGTAAGCTCCTAATC | 33 bp deleted → no frameshift |
| 149 | CTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCCTAATC | 14 bp deleted → frameshift |
| 147 | CTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCTCCTAATC | 20 bp deleted → frameshift |
| 146 | CTCCATCCCC----------------------------------AAGTAAGCTCCTAATC | 34 bp deleted → frameshift |
| 143 | CTCCATCCCCGGACTCTCCA-------------------------AGTAAGCTCCTAATC | 25 bp deleted → frameshift |
| 140 | CTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTCCTAATC | 21 bp deleted → no frameshift |
| 133 | CTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCCTAATC | 22 bp deleted → frameshift |
| 133 | CTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCCTAATC | 22 bp deleted → frameshift |
| 126 | CTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCCTAATC | 23 bp deleted → frameshift |
| 121 | CTCCATCCCC--------------------------------CCAAGTAAGCTCCTAATC | 32 bp deleted → frameshift |
| 120 | CTCCATCCCCGGACTCTCC------------------------CAAGTAAGCTCCTAATC | 24 bp deleted → no frameshift |
| 115 | CTCCATCCCCGGACTCTCC------------------------------------TAATC | 36 bp deleted → no frameshift |
| 104 | CTCCATCCC-----------------------------------AAGTAAGCTCCTAATC | 35 bp deleted → frameshift |
| 90 | CTCCATCCCCGGACTCTCCAGAG------------------------TAAGCTCCTAATC | 24 bp deleted → no frameshift |
| 84 | CTCCATCCCC-------------------------------CCCAAGTAAGCTCCTAATC | 31 bp deleted → frameshift |
| 81 | CTCCATCCCCGGACTCTCCAG--------------------------TAAGCTCCTAATC | 26 bp deleted → frameshift |
| 81 | CTCCATCCCCGGAC--------------------------CCCCAAGTAAGCTCCTAATC | 26 bp deleted → frameshift |
| 75 | CTCCATCCCCGGACTC--------------------------------------CTAATC | 38 bp deleted → frameshift |
| 74 | CTCCATCCCCGGACTCTCCAGAG----------------------------CTCCTAATC | 28 bp deleted → frameshift |
| 67 | CTCCA----------------------------------------AGTAAGCTCCTAATC | 40 bp deleted → frameshift |
| 66 | CTCCATCCCCGGACTCTCCAG------------------------------CTCCTAATC | 30 bp deleted → no frameshift |
| 66 | CTCCATCC------------------------------------AAGTAAGCTCCTAATC | 36 bp deleted → no frameshift |
| 62 | CTCC-------------------------------------CCCAAGTAAGCTCCTAATC | 37 bp deleted → frameshift |
| 60 | CTCC--------------------------------------CCAAGTAAGCTCCTAATC | 38 bp deleted → frameshift |
| 56 | CTCC---------------------------------------CAAGTAAGCTCCTAATC | 39 bp deleted → no frameshift |
| 54 | CTCC---------------------------------------------------TAATC | 51 bp deleted → no frameshift |
| 47 | CTCCATCC-----------------------------------------------TAATC | 47 bp deleted → frameshift |
| 42 | CTCCATCCCCGGACTCT---------------------------------------AATC | 39 bp deleted → no frameshift |
| 42 | CTCCATCCCC---------------------------------------------TAATC | 45 bp deleted → no frameshift |
| 40 | CTCCATCCC----------------------------------------------TAATC | 46 bp deleted → frameshift |
| 38 | CTCCATCCCCGGACT-----------------------------------------AATC | 41 bp deleted → frameshift |
| 36 | CTCCATCCCCGGACTCTC------------------------------------------ | 42 bp deleted → no frameshift |
| 33 | CTCCATCCCCGGACTC-------------------------------------------- | 44 bp deleted → frameshift |
| 28 | CTCCATC----------------------------------------------------- | 53 bp deleted → frameshift |
| 20 | CT------------------------------------------------------AATC | 54 bp deleted → no frameshift |
| 17 | CTC--------------------------------------------------------- | 57 bp deleted → no frameshift |