CRISPOR

DNA break repair outcome predictions

Guide: TAGCCCCAGCGCCGTGTCGA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCT`	(Wild-type sequence)
370	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCGA------CCGCGGAGATGGCAGTGGGGAGCT`	`6 bp deleted → no frameshift`
344	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCG---GCGACCGCGGAGATGGCAGTGGGGAGCT`	`3 bp deleted → no frameshift`
298	`AGGCGCCGGGTAGCCCCAGCG--------------ACCGCGGAGATGGCAGTGGGGAGCT`	`14 bp deleted → frameshift`
283	`AGGCGCCGGGTAGCCCCAGCGCCG---------------CGGAGATGGCAGTGGGGAGCT`	`15 bp deleted → no frameshift`
268	`AGGCGCCGGGTAGCCCCAGCGCCG--------GCGACCGCGGAGATGGCAGTGGGGAGCT`	`8 bp deleted → frameshift`
244	`AGGCGCCGGGTAGCCCCAGCGC------------------GGAGATGGCAGTGGGGAGCT`	`18 bp deleted → no frameshift`
242	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCG----------CGGAGATGGCAGTGGGGAGCT`	`10 bp deleted → frameshift`
230	`AGGCGCCGGGTAGCCCCAGCGCCG-----------ACCGCGGAGATGGCAGTGGGGAGCT`	`11 bp deleted → frameshift`
230	`AGGCGCCGGGTAGCCCCAGCG-----------GCGACCGCGGAGATGGCAGTGGGGAGCT`	`11 bp deleted → frameshift`
220	`AGGCGCCGGGTAGCCCCAGCG--------------------GAGATGGCAGTGGGGAGCT`	`20 bp deleted → frameshift`
219	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCG------------GAGATGGCAGTGGGGAGCT`	`12 bp deleted → no frameshift`
219	`AGGCGCCGGGTAGCCCCAGCGC------------GACCGCGGAGATGGCAGTGGGGAGCT`	`12 bp deleted → no frameshift`
180	`AGGCGCCGG------------------------CGACCGCGGAGATGGCAGTGGGGAGCT`	`24 bp deleted → no frameshift`
178	`AGGCG------------------------------ACCGCGGAGATGGCAGTGGGGAGCT`	`30 bp deleted → no frameshift`
170	`AGGCGCCGGGTAGCCCCAGCGCCG-----------------GAGATGGCAGTGGGGAGCT`	`17 bp deleted → frameshift`
156	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCGA-------------GATGGCAGTGGGGAGCT`	`13 bp deleted → frameshift`
147	`AGGCGCCGGGTAGC--------------------GACCGCGGAGATGGCAGTGGGGAGCT`	`20 bp deleted → frameshift`
141	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCGA---------------TGGCAGTGGGGAGCT`	`15 bp deleted → no frameshift`
140	`AGGCGCCGGGTAGCCCC---------------------GCGGAGATGGCAGTGGGGAGCT`	`21 bp deleted → no frameshift`
133	`AGGCGCCGGGTAGCCC----------------------GCGGAGATGGCAGTGGGGAGCT`	`22 bp deleted → frameshift`
129	`AGGCGCCGGGTAGCCCCAGCGCCGTG---------------------------GGGAGCT`	`27 bp deleted → no frameshift`
127	`AGGCGCCG-------------------------------CGGAGATGGCAGTGGGGAGCT`	`31 bp deleted → frameshift`
126	`AGGCGCCGGGTAGCC-----------------------GCGGAGATGGCAGTGGGGAGCT`	`23 bp deleted → frameshift`
126	`AGGCGCCGGG-----------------------CGACCGCGGAGATGGCAGTGGGGAGCT`	`23 bp deleted → frameshift`
115	`AGGCGCCGG---------------------------------AGATGGCAGTGGGGAGCT`	`33 bp deleted → no frameshift`
109	`AGGCGC----------------------------------GGAGATGGCAGTGGGGAGCT`	`34 bp deleted → frameshift`
109	`AGGCGCCGGGTAGC--------------------------GGAGATGGCAGTGGGGAGCT`	`26 bp deleted → frameshift`
105	`AGGCGCCGGGTAGCCCCAGCGCCGTG---------------------GCAGTGGGGAGCT`	`21 bp deleted → no frameshift`
103	`AGGCGCCGGGTAGCCCCAGCGC---------------------------AGTGGGGAGCT`	`27 bp deleted → no frameshift`
103	`AGGCGCCG---------------------------ACCGCGGAGATGGCAGTGGGGAGCT`	`27 bp deleted → no frameshift`
103	`AGGCG---------------------------GCGACCGCGGAGATGGCAGTGGGGAGCT`	`27 bp deleted → no frameshift`
101	`AGGCGCCGGGTAGCCCCAG--------------------------------TGGGGAGCT`	`32 bp deleted → frameshift`
99	`AGGCG------------------------------------GAGATGGCAGTGGGGAGCT`	`36 bp deleted → no frameshift`
98	`AGGCGC----------------------------GACCGCGGAGATGGCAGTGGGGAGCT`	`28 bp deleted → frameshift`
94	`AGGCGCCGGGTAGCCCCAGC-----------------------------AGTGGGGAGCT`	`29 bp deleted → frameshift`
86	`AGGCGCCGGGTAGCCCCAGCGCCGTGT-------------------------GGGGAGCT`	`25 bp deleted → frameshift`
86	`AGGCGCCGGGTAGCCCCAG-------------------------ATGGCAGTGGGGAGCT`	`25 bp deleted → frameshift`
80	`AGGCGCCGGG--------------------------------AGATGGCAGTGGGGAGCT`	`32 bp deleted → frameshift`
77	`AGGCGCCGGGTAGCCCCAGCGCCGTGTCGA---------------------------GCT`	`27 bp deleted → no frameshift`
71	`AGGCGCCGGGTAGCCCCAGC---------------------------------------T`	`39 bp deleted → no frameshift`
69	`AGGCGCCGGGTAGC-----------------------------------AGTGGGGAGCT`	`35 bp deleted → frameshift`
63	`AGGCGCCGGGTAG-------------------------------ATGGCAGTGGGGAGCT`	`31 bp deleted → frameshift`
63	`AGGCGCCGGG---------------------------------------------GAGCT`	`45 bp deleted → no frameshift`
63	`AGGC---------------------------------------------AGTGGGGAGCT`	`45 bp deleted → no frameshift`
62	`AGGCGCCGGGTAGCCCCAGCGC-------------------------------------T`	`37 bp deleted → frameshift`
60	`AGGCGCCGGG--------------------------------------CAGTGGGGAGCT`	`38 bp deleted → frameshift`
60	`AGGCGCCGGG----------------------------------------------AGCT`	`46 bp deleted → frameshift`
56	`AGGCGCCGG---------------------------------------CAGTGGGGAGCT`	`39 bp deleted → no frameshift`
56	`AGG---------------------------------------AGATGGCAGTGGGGAGCT`	`39 bp deleted → no frameshift`
52	`AGGCGCCGGGTAGC---------------------------------------------T`	`45 bp deleted → no frameshift`
46	`AGGCGC-------------------------------------------AGTGGGGAGCT`	`43 bp deleted → frameshift`
45	`AGGCGCCGGGTAG--------------------------------------TGGGGAGCT`	`38 bp deleted → frameshift`
44	`AGGCGCCGGG--------------------------------------------GGAGCT`	`44 bp deleted → frameshift`
38	`AGGCGCCGGGT-----------------------------------------GGGGAGCT`	`41 bp deleted → frameshift`
38	`AGGCGCCGG-----------------------------------------------AGCT`	`47 bp deleted → frameshift`
36	`AG------------------------------------------ATGGCAGTGGGGAGCT`	`42 bp deleted → no frameshift`
31	`AGG---------------------------------------------------GGAGCT`	`51 bp deleted → no frameshift`
29	`AGG----------------------------------------------------GAGCT`	`52 bp deleted → frameshift`
28	`AGGCGC-----------------------------------------------------T`	`53 bp deleted → frameshift`
28	`AGG-----------------------------------------------------AGCT`	`53 bp deleted → frameshift`
25	`AG-------------------------------------------------TGGGGAGCT`	`49 bp deleted → frameshift`
25	`AGGC-------------------------------------------------------T`	`55 bp deleted → frameshift`
18	`AG--------------------------------------------------------CT`	`56 bp deleted → frameshift`

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