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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314131_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314131_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intron_Lrrfip1_chr1_91044536_F | Primer3: not found at this Tm | N.d. | 0.71 |
| mm4_intron_Lrrfip1_chr1_91044536_R | Primer3: not found at this Tm | N.d. | 0.71 |
| mm4_intergenic_Gm23665|Csmd3_chr15_47139645_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Gm23665|Csmd3_chr15_47139645_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Dcx|Gm15048_chrX_143981415_F | Primer3: not found at this Tm | N.d. | 0.49 |
| mm4_intergenic_Dcx|Gm15048_chrX_143981415_R | Primer3: not found at this Tm | N.d. | 0.49 |
| mm4_exon_Proser2_chr2_6130045_F | TCGTCGGCAGCGTCCTCAGTAGGGCGGAACTCAC | 59.8 | 0.49 |
| mm4_exon_Proser2_chr2_6130045_R | GTCTCGTGGGCTCGGGTCTCAGTCGGCGTGGAC | 60.1 | 0.49 |
| mm3_intergenic_Zmynd8|Gm22692_chr2_165906508_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm3_intergenic_Zmynd8|Gm22692_chr2_165906508_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327976_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327976_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm3_intergenic_Cadm2|Gm15828_chr16_67242556_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm3_intergenic_Cadm2|Gm15828_chr16_67242556_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Ptgs2|Gm26687_chr1_150145766_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Ptgs2|Gm26687_chr1_150145766_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_1700082M22Rik|Ces2a_chr8_104673716_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_1700082M22Rik|Ces2a_chr8_104673716_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_RP23-302F9.3_chr8_87235430_F | TCGTCGGCAGCGTCCCCAGTGCAGAACTCTCCTC | 59.7 | 0.30 |
| mm4_intron_RP23-302F9.3_chr8_87235430_R | GTCTCGTGGGCTCGGATCTGCATTCCTGGAAGGCC | 60.1 | 0.30 |
| mm4_intron_Mdga1_chr17_29966728_F | TCGTCGGCAGCGTCCTTGATGTGAGGAGGGGCTG | 60.1 | 0.28 |
| mm4_intron_Mdga1_chr17_29966728_R | GTCTCGTGGGCTCGGACCAACCACAGTCCTCTCTG | 58.9 | 0.28 |
| mm4_intron_Ispd_chr12_36517778_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Ispd_chr12_36517778_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Foxa2|AL845297.1_chr2_148052873_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Foxa2|AL845297.1_chr2_148052873_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Gm10247|Grin2a_chr16_9440032_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Gm10247|Grin2a_chr16_9440032_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Plxnc1_chr10_94832963_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Plxnc1_chr10_94832963_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Meis3_chr7_16178029_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Meis3_chr7_16178029_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ano1_chr7_144678048_F | TCGTCGGCAGCGTCGCTAAGGGCCTGGAAAGGAG | 60.1 | 0.25 |
| mm4_intron_Ano1_chr7_144678048_R | GTCTCGTGGGCTCGGTAGAGTGTCTCAGGGTCCCC | 59.6 | 0.25 |
| mm4_intron_Ros1_chr10_52154094_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ros1_chr10_52154094_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_Katnal1_chr5_148876226_F | TCGTCGGCAGCGTCGCTGAGACAGACTTGGCGAT | 60.1 | 0.24 |
| mm4_exon_Katnal1_chr5_148876226_R | GTCTCGTGGGCTCGGGCGCATCAATGGCTTGAGTC | 60.2 | 0.24 |
| mm4_intergenic_1700116B05Rik|Gm24925_chr16_50848040_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_1700116B05Rik|Gm24925_chr16_50848040_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Exosc8_chr3_54734805_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Exosc8_chr3_54734805_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Tg_chr15_66720749_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Tg_chr15_66720749_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm12930|Rspo1_chr4_124965177_F | TCGTCGGCAGCGTCACAGGTGCTTTGAGGGTCAG | 59.8 | 0.22 |
| mm4_intergenic_Gm12930|Rspo1_chr4_124965177_R | GTCTCGTGGGCTCGGGGACTCACAAGAGGTCAGGC | 60.0 | 0.22 |
| mm4_intergenic_Gm24256|Grik2_chr10_48666508_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm24256|Grik2_chr10_48666508_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Clpb_chr7_101751537_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Clpb_chr7_101751537_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intergenic_Gtf3c2|Eif2b4_chr5_31182965_F | TCGTCGGCAGCGTCGGAGACCCAGTACATAGAAGCA | 58.9 | 0.20 |
| mm3_intergenic_Gtf3c2|Eif2b4_chr5_31182965_R | GTCTCGTGGGCTCGGAGTCCTACTGGAAAGGAAGGA | 57.4 | 0.20 |
| mm4_intergenic_Gpr151|Ppp2r2b_chr18_42621917_F | TCGTCGGCAGCGTCTTAACAAGGTGCTGCGGAGT | 59.8 | 0.19 |
| mm4_intergenic_Gpr151|Ppp2r2b_chr18_42621917_R | GTCTCGTGGGCTCGGAGCCTGAGGGTGAGAAGGAT | 59.9 | 0.19 |
| mm3_intergenic_Lcn2|Ptges2_chr2_32395649_F | TCGTCGGCAGCGTCTTCCACGCCCACTATAAGGC | 59.8 | 0.17 |
| mm3_intergenic_Lcn2|Ptges2_chr2_32395649_R | GTCTCGTGGGCTCGGACTTGTCTTAACGGAGCGCC | 60.6 | 0.17 |
| mm4_intron_Fer1l5_chr1_36396918_F | TCGTCGGCAGCGTCTTTCCATCCCGATCACAGCC | 60.1 | 0.17 |
| mm4_intron_Fer1l5_chr1_36396918_R | GTCTCGTGGGCTCGGAGACAATTCACTGAGGCACA | 57.0 | 0.17 |
| mm3_exon_Mospd3_chr5_137599738_F | TCGTCGGCAGCGTCCTGGGGCTCGAAGAACAGAG | 60.1 | 0.17 |
| mm3_exon_Mospd3_chr5_137599738_R | GTCTCGTGGGCTCGGCAGCCACTATGACGTCCAGG | 60.1 | 0.17 |
| mm4_intron_Faim2_chr15_99525142_F | TCGTCGGCAGCGTCTCCACCAGAGTGCCTATGGA | 59.9 | 0.16 |
| mm4_intron_Faim2_chr15_99525142_R | GTCTCGTGGGCTCGGAGGTCTCCTATGGGTCAGGC | 60.3 | 0.16 |
| mm4_intron_Tjp2_chr19_24206747_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Tjp2_chr19_24206747_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Sv2c_chr13_95973060_F | TCGTCGGCAGCGTCGAGTGCATGGGTTGGTGACA | 60.5 | 0.15 |
| mm3_intron_Sv2c_chr13_95973060_R | GTCTCGTGGGCTCGGGACCACTCAGCTAGCCTGTG | 60.1 | 0.15 |
| mm3_intron_Myo7a_chr7_98078646_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_Myo7a_chr7_98078646_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477295_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477295_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_H1f0_chr15_79028388_F | TCGTCGGCAGCGTCGAAGAAACACAGATGGCGGC | 59.8 | 0.15 |
| mm4_exon_H1f0_chr15_79028388_R | GTCTCGTGGGCTCGGTTTTCCCAGCTCTGCGTCTG | 60.6 | 0.15 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442367_F | TCGTCGGCAGCGTCCCAGCCTCCAAAACCTAAGC | 58.8 | 0.15 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442367_R | GTCTCGTGGGCTCGGACAGGAAGAAACCGGCTGAG | 59.9 | 0.15 |
| mm3_intron_Pparg_chr6_115361421_F | TCGTCGGCAGCGTCGTGACAGACAAGGTGAGGGG | 59.9 | 0.15 |
| mm3_intron_Pparg_chr6_115361421_R | GTCTCGTGGGCTCGGTACGCAGCCCTGTCAGAATG | 60.1 | 0.15 |
| mm4_intron_Otud7a_chr7_63753331_F | TCGTCGGCAGCGTCACAAGACAGGCTGAGCTCAG | 59.6 | 0.13 |
| mm4_intron_Otud7a_chr7_63753331_R | GTCTCGTGGGCTCGGGGTTTTCCTTGTGTGTGGCC | 59.8 | 0.13 |
| mm4_intergenic_Gm14647|Gm25851_chrX_58326549_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm14647|Gm25851_chrX_58326549_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Asic4_chr1_75473279_F | TCGTCGGCAGCGTCCTGCAAGGTCTCCTGGGATC | 59.8 | 0.13 |
| mm4_exon_Asic4_chr1_75473279_R | GTCTCGTGGGCTCGGTGTTCCTTCAGCTCCTGCAG | 59.9 | 0.13 |
| mm4_intergenic_Gm10087|Sbf2_chr7_110557755_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm10087|Sbf2_chr7_110557755_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Kcnma1|Gm6158_chr14_23945497_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Kcnma1|Gm6158_chr14_23945497_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563867_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563867_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm25845|Celf4_chr18_25622806_F | TCGTCGGCAGCGTCTGGGGAGCCATAGAGGGAAA | 59.9 | 0.11 |
| mm4_intergenic_Gm25845|Celf4_chr18_25622806_R | GTCTCGTGGGCTCGGGGCTATAGTGAGGTGGGTGG | 59.2 | 0.11 |
| mm4_intergenic_Gm4294|Gm26171_chr12_12024002_F | TCGTCGGCAGCGTCCAGAAGAACGGGTAGGCGTT | 60.0 | 0.10 |
| mm4_intergenic_Gm4294|Gm26171_chr12_12024002_R | GTCTCGTGGGCTCGGTTGCAGCAGTCACGAATCCA | 60.2 | 0.10 |
| mm4_intergenic_Gm25278|Csmd1_chr8_16966799_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm25278|Csmd1_chr8_16966799_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Prrt2_chr7_127021021_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Prrt2_chr7_127021021_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Acoxl_chr2_127926811_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Acoxl_chr2_127926811_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pamr1_chr2_102633653_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pamr1_chr2_102633653_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340809_F | TCGTCGGCAGCGTCTTCTAGGGAAGCAGGGACCA | 59.8 | 0.09 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340809_R | GTCTCGTGGGCTCGGGGTGGGCTTTCTGTCTCTGG | 60.3 | 0.09 |
| mm4_intergenic_Rb1|Itm2b_chr14_73327847_F | TCGTCGGCAGCGTCAAATAAATGGGGCAGCGCTG | 59.5 | 0.09 |
| mm4_intergenic_Rb1|Itm2b_chr14_73327847_R | GTCTCGTGGGCTCGGTCCCTGCCTCCATTTCTCCT | 60.2 | 0.09 |
| mm4_exon_Katnal1_chr5_148926798_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Katnal1_chr5_148926798_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Syn2|Gm17482_chr6_115208373_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Syn2|Gm17482_chr6_115208373_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Mpl|Tie1_chr4_118457525_F | TCGTCGGCAGCGTCATCTTCTCCGGCACTGTGTG | 60.0 | 0.09 |
| mm4_intergenic_Mpl|Tie1_chr4_118457525_R | GTCTCGTGGGCTCGGGACGTGGGGCTGTATCTGAC | 60.1 | 0.09 |
| mm4_intergenic_Bre|Gm10463_chr5_32089196_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Bre|Gm10463_chr5_32089196_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740728_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740728_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154619323_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154619323_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13403|Gm13404_chr2_33974429_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13403|Gm13404_chr2_33974429_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm23943|4930558J18Rik_chr1_57250010_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm23943|4930558J18Rik_chr1_57250010_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_exon_Gramd2_chr9_59680149_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_exon_Gramd2_chr9_59680149_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Kifc3|Cngb1_chr8_95174496_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Kifc3|Cngb1_chr8_95174496_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Foxi3|Gm23485_chr6_70985308_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Foxi3|Gm23485_chr6_70985308_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Rnf38_chr4_44233164_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Rnf38_chr4_44233164_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Llgl2_chr11_115824678_F | TCGTCGGCAGCGTCTGTGCCTCCTAATCCTCCGA | 60.0 | 0.07 |
| mm4_intron_Llgl2_chr11_115824678_R | GTCTCGTGGGCTCGGGTGTCCAAAGTCGCTCTGGA | 59.9 | 0.07 |
| mm4_intergenic_Hlcs|Ripply3_chr16_94328373_F | TCGTCGGCAGCGTCGAGCCTTTCTGGTCTCCACC | 60.0 | 0.06 |
| mm4_intergenic_Hlcs|Ripply3_chr16_94328373_R | GTCTCGTGGGCTCGGACTCGGCGCCAGGTTTAAAA | 60.5 | 0.06 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109034501_F | TCGTCGGCAGCGTCGGATACCTAGGCCTTCCCTCT | 59.8 | 0.06 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109034501_R | GTCTCGTGGGCTCGGAGAGCCACTGACCTTTGGT | 58.4 | 0.06 |
| mm4_exon_Zbtb7b_chr3_89378879_F | TCGTCGGCAGCGTCGATGGCTCCGTCCTTACAGG | 59.8 | 0.05 |
| mm4_exon_Zbtb7b_chr3_89378879_R | GTCTCGTGGGCTCGGGGGTGGCAAAGGAAGGGTAA | 59.8 | 0.05 |
| mm4_intergenic_Gm26919|Gm6600_chr6_130706173_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26919|Gm6600_chr6_130706173_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Sema4g_chr19_45001624_F | TCGTCGGCAGCGTCGTGGCTTGTCTGAAGGGAGG | 60.3 | 0.04 |
| mm4_exon_Sema4g_chr19_45001624_R | GTCTCGTGGGCTCGGCACTGGCCTGAGACAGTCTG | 60.0 | 0.04 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118553039_F | TCGTCGGCAGCGTCGAGGCAAACATGCGCAGAAA | 60.0 | 0.04 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118553039_R | GTCTCGTGGGCTCGGAACTGGGGTCTCTGCAAAGC | 60.5 | 0.04 |
| mm3_intron_Zfhx3_chr8_108900930_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Zfhx3_chr8_108900930_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Syt7_chr19_10424209_F | TCGTCGGCAGCGTCCACAAGCCCACTTCCTCCTG | 60.6 | 0.04 |
| mm4_intron_Syt7_chr19_10424209_R | GTCTCGTGGGCTCGGCCAGTGAGCAAATGCCATGG | 59.8 | 0.04 |
| mm4_intergenic_Casr|Cd86_chr16_36592639_F | TCGTCGGCAGCGTCCCCAGGACTCTCAACAGTGT | 58.9 | 0.04 |
| mm4_intergenic_Casr|Cd86_chr16_36592639_R | GTCTCGTGGGCTCGGAGGAATGTCACAATGGCCCA | 59.5 | 0.04 |
| mm4_intergenic_Timm10|Slc43a1_chr2_84831373_F | TCGTCGGCAGCGTCCCACCCACCCAGGTAAAGG | 59.6 | 0.03 |
| mm4_intergenic_Timm10|Slc43a1_chr2_84831373_R | GTCTCGTGGGCTCGGGCTTCATTCTGAAAGCTGCAGT | 59.7 | 0.03 |
| mm4_exon_Rassf1_chr9_107554737_F | TCGTCGGCAGCGTCCGCGACCTGACACACTCA | 59.6 | 0.03 |
| mm4_exon_Rassf1_chr9_107554737_R | GTCTCGTGGGCTCGGAGGAAGGTGTTTCAGCCTCG | 59.9 | 0.03 |
| mm4_intron_Colgalt1_chr8_71611529_F | TCGTCGGCAGCGTCACTAGGCGGGACTCAAGTCT | 59.9 | 0.03 |
| mm4_intron_Colgalt1_chr8_71611529_R | GTCTCGTGGGCTCGGGTGCGTAATCCCTGGGGTAC | 60.1 | 0.03 |
| mm4_intron_Sntb2_chr8_106943037_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sntb2_chr8_106943037_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pik3ip1/Gm12592|Pik3ip1_chr11_3331336_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pik3ip1/Gm12592|Pik3ip1_chr11_3331336_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172627571_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172627571_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm14300|Cdh4_chr2_179704836_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm14300|Cdh4_chr2_179704836_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540511_F | TCGTCGGCAGCGTCTGGGCTGATAATGGCTGGAG | 59.5 | 0.02 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540511_R | GTCTCGTGGGCTCGGCCGTCAGGTTGTGTTCGTTG | 59.6 | 0.02 |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12652660_F | TCGTCGGCAGCGTCACAACTCAACACGGGCACA | 60.0 | 0.02 |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12652660_R | GTCTCGTGGGCTCGGCAGCCCAGATGGAGCTCTC | 59.5 | 0.02 |
| mm4_intergenic_Tab1|Mgat3_chr15_80173476_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tab1|Mgat3_chr15_80173476_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fnta|Hook3_chr8_26015615_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fnta|Hook3_chr8_26015615_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Gm3764_chr3_88224950_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Gm3764_chr3_88224950_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gsx2|Pdgfra_chr5_75085052_F | TCGTCGGCAGCGTCTTGGGTTTGAAAGGCTGGGT | 60.0 | 0.02 |
| mm4_intergenic_Gsx2|Pdgfra_chr5_75085052_R | GTCTCGTGGGCTCGGAGCAGCTGATCTGAAGGACG | 59.8 | 0.02 |
| mm4_intron_Sdr42e1_chr8_117664139_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Sdr42e1_chr8_117664139_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lcor_chr19_41483375_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lcor_chr19_41483375_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_1700071K01Rik|Gm11418_chr11_81582666_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_1700071K01Rik|Gm11418_chr11_81582666_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460429_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460429_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm4258|Ptprc_chr1_138057591_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm4258|Ptprc_chr1_138057591_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Rapgef3/Gm16256_chr15_97767735_F | TCGTCGGCAGCGTCACAGGGCGTTCAAGCGAAG | 60.9 | 0.02 |
| mm4_exon_Rapgef3/Gm16256_chr15_97767735_R | GTCTCGTGGGCTCGGTACCGTAAAGCTCGTGCCTG | 60.1 | 0.02 |
| mm4_intron_Hsf2bp_chr17_32018676_F | TCGTCGGCAGCGTCGAGTTGGGACTGGACACCTG | 59.9 | 0.02 |
| mm4_intron_Hsf2bp_chr17_32018676_R | GTCTCGTGGGCTCGGGCCTGTACCACAGCTCTGAG | 60.1 | 0.02 |
| mm4_intron_Ppp3ca_chr3_136730177_F | TCGTCGGCAGCGTCGCTGAAGCTGACAGGGATGT | 60.0 | 0.01 |
| mm4_intron_Ppp3ca_chr3_136730177_R | GTCTCGTGGGCTCGGAGGGCCTCTTCACGAGTTTG | 59.9 | 0.01 |
| mm4_intron_4930506M07Rik_chr19_58986158_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_4930506M07Rik_chr19_58986158_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Syt12_chr19_4476936_F | TCGTCGGCAGCGTCGAAGAGGGACTGAGTGCGTC | 60.1 | 0.01 |
| mm4_exon_Syt12_chr19_4476936_R | GTCTCGTGGGCTCGGGGGAGCAGAGATAGAACCAGG | 59.3 | 0.01 |
| mm4_intron_Prickle2_chr6_92706048_F | TCGTCGGCAGCGTCGCTTACCTGGGGCCACAG | 60.0 | 0.01 |
| mm4_intron_Prickle2_chr6_92706048_R | GTCTCGTGGGCTCGGCTCGGTGAGTGTCCTCCAC | 59.4 | 0.01 |
| mm4_exon_Cdh15_chr8_122861997_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Cdh15_chr8_122861997_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Col4a2_chr8_11350110_F | TCGTCGGCAGCGTCCCAAGCTGATGGCAAGGAGA | 60.0 | 0.01 |
| mm4_intron_Col4a2_chr8_11350110_R | GTCTCGTGGGCTCGGTAGCCCATCCAATCATCCGC | 59.9 | 0.01 |
| mm4_intergenic_Vps45|Otud7b_chr3_96082162_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Vps45|Otud7b_chr3_96082162_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Rgma_chr7_73409351_F | TCGTCGGCAGCGTCGGAGTTCTGTGCAGCCCTG | 60.6 | 0.00 |
| mm4_exon_Rgma_chr7_73409351_R | GTCTCGTGGGCTCGGCATGGACAGCCGAGTGGTAA | 59.7 | 0.00 |
| mm4_intron_Eif3h_chr15_51793086_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Eif3h_chr15_51793086_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kalrn_chr16_33990771_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kalrn_chr16_33990771_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Clstn2_chr9_97664756_F | TCGTCGGCAGCGTCAAAAGCCACAAAGCAGCAGG | 59.8 | 0.00 |
| mm4_intron_Clstn2_chr9_97664756_R | GTCTCGTGGGCTCGGTCCCTGGGCAAGCATTCTAC | 59.7 | 0.00 |
| mm4_intergenic_Id2|Gm22527_chr12_25374329_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Id2|Gm22527_chr12_25374329_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23786|Rbbp7_chrX_162760181_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23786|Rbbp7_chrX_162760181_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594432_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594432_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Elac1|Me2_chr18_73758012_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Elac1|Me2_chr18_73758012_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Atg2b_chr12_105684859_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Atg2b_chr12_105684859_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_9630013D21Rik/Gm12811_chr4_109657168_F | TCGTCGGCAGCGTCGGGAGGAAGGGAGAGCTACT | 59.7 | 0.00 |
| mm4_exon_9630013D21Rik/Gm12811_chr4_109657168_R | GTCTCGTGGGCTCGGGCGAGCTGAGGAGAGCAG | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314131 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_exon_Proser2_chr2_6130045 | CTCAGTAGGGCGGAACTCACCGAGCCAAGTCCCCTCCGCGGGTCGCAGGTGTCCACGCCGACTGAGAC |
| mm4_intron_RP23-302F9.3_chr8_87235430 |
CCCAGTGCAGAACTCTCCTCATCCCAGTTCCCTCCAGTGACAGCCTCACCTCACATGCTAAGGAAGGAACTGCACTAACC ACTTATCAACCCTCCCCCAGGGGCCTTCCAGGAATGCAGAT |
| mm4_intron_Mdga1_chr17_29966728 |
CTTGATGTGAGGAGGGGCTGGGGGGGGGGGGGAGAATGGCTGGGGAGGGTGGGCATATGGCTGTCCCAGGGGAGGACTGG GTACAGAGAGGACTGTGGTTGGT |
| mm4_intron_Ano1_chr7_144678048 |
GCTAAGGGCCTGGAAAGGAGGGTCTCATCCAGGCTTGGGCTGTATGCGTAGGAGACTGGGAACCCCCGGACTGGGCACTG TGGGGACCCTGAGACACTCTA |
| mm4_exon_Katnal1_chr5_148876226 |
GCTGAGACAGACTTGGCGATTTTCTTCAGAGCCAACTCCAAGTCCCCTCTGGTGACAGGCATCTGCAGCTCCTCCTTGGA CAGGGCCCGGATCTCTTCCGGACTCAAGCCATTGATGCGC |
| mm4_intergenic_Gm12930|Rspo1_chr4_124965177 |
ACAGGTGCTTTGAGGGTCAGGATGGGGGTGGTCGGAGGCCTAGCCTCCTCCGCGGATGGCCTCCTGGAAGACTGGCTTGG ATATAGCTAGGCAGGGGGCAGTGCTGCCTGACCTCTTGTGAGTCC |
| mm3_intergenic_Gtf3c2|Eif2b4_chr5_31182965 |
GGAGACCCAGTACATAGAAGCACTTGGAAGGCCAAGTGCCCTTCACGGACAGCCTCGCCCTGGCTGAAAAACAAAAACTA GAGAGTATAAAAGAACAGTGAAGTATTTATCCTTCCTTTCCAGTAGGACT |
| mm4_intergenic_Gpr151|Ppp2r2b_chr18_42621917 |
TTAACAAGGTGCTGCGGAGTCCAAGTCCCCTCCTCGCCCAGTCCCTTCCTCGCCCCTACACGGTTCTGGAGACACTAATT TGACACCGTTATATCCTTCTCACCCTCAGGCT |
| mm3_intergenic_Lcn2|Ptges2_chr2_32395649 |
TTCCACGCCCACTATAAGGCCCCGCCCTCTTTCGGCTTCGGGGCGGGGTCAAGTCAGGGCTGTCCGCGGAGAAGCCTAGG CAGTGGAGGTCGGGCGCTCCGTTAAGACAAGT |
| mm4_intron_Fer1l5_chr1_36396918 |
TTTCCATCCCGATCACAGCCCCCCTCCTCCCAGACCCCTCCTCACACAGCCCCCCCTCCCCACCCCCACTCACTTTTTGA TTTTGTATTTTCATTTCATTTTGGTCACTTGAGAAGTTTTTGTGCCTCAGTGAATTGTCT |
| mm3_exon_Mospd3_chr5_137599738 |
CTGGGGCTCGAAGAACAGAGGTGATGTCTTTTCTTCCAACCACTCGTCCCTCAGTCCCCTCCTCAGACAGCTCAATGCGG AATCTGTCCTGGACGTCATAGTGGCTG |
| mm4_intron_Faim2_chr15_99525142 |
TCCACCAGAGTGCCTATGGAGGCCATAGGGCCATCCGGGGAAGGGACTGGGCCTCTGTGGTCCTACAGTTGCCTGACCCA TAGGAGACCT |
| mm3_intron_Sv2c_chr13_95973060 |
GAGTGCATGGGTTGGTGACAAGAAGGCTGTCAGTAGAGGGGACTCAGCATGACATTTTCTTCATCTTCATCCCACATCTC AAACAATCACAGCACAGGCTAGCTGAGTGGTC |
| mm4_exon_H1f0_chr15_79028388 |
GAAGAAACACAGATGGCGGCAGCGCGGCGCCATTCCGGGCCGGGAGCAGGCAGCCAGCAGCCCAGTCCTCACCGCGGTCC GCCCGCCGCCGCTAAATACCCGGATGCGCCGCCCGAGCGCCAGACGCAGAGCTGGGAAAA |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442367 |
CCAGCCTCCAAAACCTAAGCTTTATAAAAAGCCATTAGCCATAGTGTCCCCAAAGAAGCTTCTAGAGGCACGAGCCTGAC AGGGGAGGGGACTGGGGTTTGGGTCATAGGAGCATCTCCTCAGCCGGTTTCTTCCTGT |
| mm3_intron_Pparg_chr6_115361421 |
GTGACAGACAAGGTGAGGGGGGGTCTCGGCTTGTGGGGTCTGGATCTGACTGGCTAGGTGACTGGGTCTCCGCTGAGGGG ACTCGGGATGAGAAGTCACATTCTGACAGGGCTGCGTA |
| mm4_intron_Otud7a_chr7_63753331 |
ACAAGACAGGCTGAGCTCAGCCCTGGAAACGAAAGCCACATCAAAGCAGAATGAGATGTCCCAGTCCCCTCCCCCGTCAG ACCTTACCTACACTGCTCCATGGCTGGCCACACACAAGGAAAACC |
| mm4_exon_Asic4_chr1_75473279 |
CTGCAAGGTCTCCTGGGATCGGCTCAAGAGGGTGTGGCGACGGCCCAAGACCCCACTGCGGACATCCACCGGGGGCATCT CCACTCTGGGGCTGCAGGAGCTGAAGGAACA |
| mm4_intergenic_Gm25845|Celf4_chr18_25622806 |
TGGGGAGCCATAGAGGGAAACCAACCTGGCTTAGTACTTGGTGCTCTCCCAGTGTCCTCCTGGGACAGCCCCACCCACCT CACTATAGCC |
| mm4_intergenic_Gm4294|Gm26171_chr12_12024002 |
CAGAAGAACGGGTAGGCGTTTATAGGAGGCTCTGGTATAATCATTACCCATTCTCCTCCGAGCACAGCCTGGACCAAATG CCTGGATTCGTGACTGCTGCAA |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340809 |
TTCTAGGGAAGCAGGGACCACAGGAGCTCACATGGGGGAGGAGCTTCCCCTCTTTTTCCTGCTGCAGGCTGGGAAGTGGC CCGCGGCTGCCAGAGGAGGGGCCTGGGGAAGGAGACCAGAGACAGAAAGCCCACC |
| mm4_intergenic_Rb1|Itm2b_chr14_73327847 |
AAATAAATGGGGCAGCGCTGGGAATGTAGCCCAGGAGAGGGGGTGTGTGCGGAGGGGATTTGGTGGTGAAGGAGAAATGG AGGCAGGGA |
| mm4_intergenic_Mpl|Tie1_chr4_118457525 |
ATCTTCTCCGGCACTGTGTGCCTGCCTTAGCCCATCCTCCCTTCAGGAAGCTGGGCCCCAATCCCCCCCGGGGCCAGCCC CTCAGGTTCCTGTCAGATACAGCCCCACGTC |
| mm4_intron_Llgl2_chr11_115824678 |
TGTGCCTCCTAATCCTCCGACTCAGGAAGTCCGCGGGTGGGACTTGGTTTTCTCGTAGAAAGGGGAGCCTTTATATTTCC AGAGCGACTTTGGACAC |
| mm4_intergenic_Hlcs|Ripply3_chr16_94328373 |
GAGCCTTTCTGGTCTCCACCCTCTGCTTCCTCCGTCCCTTCCCGAGGTCCCTTGCCCTCCCCCTCCCCACGGGGCGCAGT TTCCCCGAGTCCCCTCCCCGCCCCGCCCCAGTTTTTAAACCTGGCGCCGAGT |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109034501 |
GGATACCTAGGCCTTCCCTCTTTGTTTTTTCAAGAGGGCAGAATGCAGTAATAACTCAAATGCCCAGGCCCCTCCCCGGA CATCATTTTTCTAACAGTCCAAGAGGAGTTGATTCAAGACCAAAGGTCAGTGGCTCT |
| mm4_exon_Zbtb7b_chr3_89378879 |
GATGGCTCCGTCCTTACAGGCCCAGTCCCCACCGCCGCCACCCCAGTCTTTCAAGCCTCTACCTTGTTTCCCCCCTCTTA CCCTTCCTTTGCCACCC |
| mm4_exon_Sema4g_chr19_45001624 |
GTGGCTTGTCTGAAGGGAGGCAGACGAGGTCGAAGACGAAAATACTCATTGGGTCGGGCTGGCCGGGCAGGGGGCTCGGC CGTGCAGCTGCAGACTGTCTCAGGCCAGTG |
| mm4_intergenic_Gm25577|Gm22537_chr12_118553039 |
GAGGCAAACATGCGCAGAAAACTCACCATTGGTCAAATGGTGTTCAATTACAGAAGCCAGGCCGGGCTGCAGCCAATGGG TGCTCTCCACGCCCCCTCCTCGTACAGCCGCTTTGCAGAGACCCCAGTT |
| mm4_intron_Syt7_chr19_10424209 |
CACAAGCCCACTTCCTCCTGATACATGGTCTCAGGAATGTCCCTAGAAGTGGATGCCTCACATCCCATCGGAGAGCCAAG GCCCCTCCCCGGGCGGCCCCGGCCCAGCTTATTGGGCCTCCATGGCATTTGCTCACTGG |
| mm4_intergenic_Casr|Cd86_chr16_36592639 |
CCCAGGACTCTCAACAGTGTTTATCCCATGCCCCCTCCGCGTCCCCTCCCCCGACAGCTTATTTCTTCTCAGTTTGGTTA GGGAAGCATCTTTCCTCCAGAAAATAATGAATGGGCCATTGTGACATTCCT |
| mm4_intergenic_Timm10|Slc43a1_chr2_84831373 |
CCACCCACCCAGGTAAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNTACTGCTGTCCGAGGAGGGCCCTGGGACTGCAGCTTTCAGAATGAAGC |
| mm4_exon_Rassf1_chr9_107554737 |
CGCGACCTGACACACTCANNNNNNNNNNNNNNNNNNNNNGCCCGAGGCGGTGGTTAGAGGCTCTCCACGCAGTGGACTAG GGATCGGGCGGGTAAGGCGATGGGCGAGGCTGAAACACCTTCCT |
| mm4_intron_Colgalt1_chr8_71611529 |
ACTAGGCGGGACTCAAGTCTCTACCGGAGGGAGGTTGCTATGCACTTGGGCTCGGCTGTGCGCTGCGGAGACTCGGTCCT CTTTCTGAGGACCTGAGTTATGTACCCCAGGGATTACGCAC |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540511 |
TGGGCTGATAATGGCTGGAGAATCCAGAGATATGCGGTCAGAATAAGTAAGGGAAGGCTGACCAGGGAGGGGAGTGGGAC TCTAGAATAAGACTGCAACGAACACAACCTGACGG |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12652660 |
ACAACTCAACACGGGCACAGCTGTCCCAGTGCCCTCGGAGGACTGCCAACATCTTTCACTTCAGGTAAATTGTTTCTGTT ATCAGATTCTTCAGACATGGAAAAAAAAAGAGTTTAAAGCGGAGAGCTCCATCTGGGCTG |
| mm4_intergenic_Gsx2|Pdgfra_chr5_75085052 |
TTGGGTTTGAAAGGCTGGGTCTAGAATAAGCGTAGGGGATGCTTCTAGGGCCTAGGGTGGCCCGAGGCCGCTCCGAGGTC AGCCGCGCCCTGCTGGCCTCCAGCTTCGCGTCCTTCAGATCAGCTGCT |
| mm4_exon_Rapgef3/Gm16256_chr15_97767735 |
ACAGGGCGTTCAAGCGAAGCTGCAGCTGGCACTGGTCCGGGCGGGAGCTGTCACACCCCCTGCGCTGCCCGCCCCTTCCC CTCCCCGGACAGGCCGAGGGGGCGGGGAGCAGGCACGAGCTTTACGGTA |
| mm4_intron_Hsf2bp_chr17_32018676 |
GAGTTGGGACTGGACACCTGCTCTTGGAGTGCAGAGGCTGGCCGGGGAGGAGACCTGGCAACAGCAAAAGCTCTCAGAGC TGTGGTACAGGC |
| mm4_intron_Ppp3ca_chr3_136730177 |
GCTGAAGCTGACAGGGATGTGGGTGTGGGCGGAGGGGAGTTGGGATTCTCTACCGGTCAGAAACAAAAACCGAAACAAAC TCGTGAAGAGGCCCT |
| mm4_exon_Syt12_chr19_4476936 |
GAAGAGGGACTGAGTGCGTCCGCCCGTCCATGCCCAGGGCGCTTGTGCCTGCCGCGCTCTGTGTGGGGGCCAGGGTGCCT ACTCACCCGGGGTTCCGCGGAGGGGACGCGGGTCCGACTCCTGGTTCTATCTCTGCTCCC |
| mm4_intron_Prickle2_chr6_92706048 |
GCTTACCTGGGGCCACAGGTGCGCGGCCGCGGGGTGGAGAGGGGCTGGGCCCGGGCGCTGGCTGCCGGCGGTGGGGACCG GGCGGGCGGCTCCGCTCGGGTGGAGGACACTCACCGAG |
| mm4_intron_Col4a2_chr8_11350110 |
CCAAGCTGATGGCAAGGAGACGTTAGTGCCCCCTCCCCTCCGCTGATAGCCACAGCCAGTCTTCTGTCTTGTTTGCAGAG CTGATCTAGTAGGCAGGGTTCGGCATCTCATATGGCGGATGATTGGATGGGCTA |
| mm4_exon_Rgma_chr7_73409351 |
GGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGCACTCGGCGGACAGCCCGCACCTGCCGGGGCGACCTGGCTTACC ACTCGGCTGTCCATG |
| mm4_intron_Clstn2_chr9_97664756 |
AAAAGCCACAAAGCAGCAGGGAAGGGTTTGTAGTGTAAGTCTTTCCTCGGAGGTGACTGGGTTGCCACTTTTCCAAAGCA TCAGTAGAATGCTTGCCCAGGGA |
| mm4_exon_9630013D21Rik/Gm12811_chr4_109657168 |
GGGAGGAAGGGAGAGCTACTCTGAGTAGGGCACCGAGTGGTGCAGCGCGCCCGCGGCTCCGGCCGCCAGCTCACCTCCGC GGCCAGCCAGGAACTCACCGGCCTGCTCTCCTCAGCTCGC |