← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313883_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313883_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm2_intergenic_4930401O12Rik|Gm11376_chr13_31284420_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm2_intergenic_4930401O12Rik|Gm11376_chr13_31284420_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Gm23821|Tgfb2_chr1_186482325_F | TCGTCGGCAGCGTCTAGGCTGCTCTGTACACCCA | 60.2 | 0.40 |
| mm4_intergenic_Gm23821|Tgfb2_chr1_186482325_R | GTCTCGTGGGCTCGGACGGAGCTTCTGCCAAGTTT | 60.1 | 0.40 |
| mm4_intergenic_Rheb|Prkag2_chr5_24859418_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Rheb|Prkag2_chr5_24859418_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Gm12983|Gm12984_chr4_135303207_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Gm12983|Gm12984_chr4_135303207_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Sirt2_chr7_28781865_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Sirt2_chr7_28781865_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Slc22a29|Slc22a30_chr19_8323154_F | TCGTCGGCAGCGTCGCTGCCAGAGTCGCATTTTC | 60.1 | 0.35 |
| mm4_intergenic_Slc22a29|Slc22a30_chr19_8323154_R | GTCTCGTGGGCTCGGTCCTTGGCATTCCCTGTGTT | 59.5 | 0.35 |
| mm4_intron_Tbc1d32_chr10_56128840_F | TCGTCGGCAGCGTCTGGTAACATGCTTTCCTGATTGT | 58.5 | 0.34 |
| mm4_intron_Tbc1d32_chr10_56128840_R | GTCTCGTGGGCTCGGGGTTCAAGACATGTAGCAGTTCC | 59.5 | 0.34 |
| mm4_intergenic_Gm24202|Gm8100_chr8_27938930_F | TCGTCGGCAGCGTCAGCTCAGCGTGCCTCTTTAA | 59.6 | 0.33 |
| mm4_intergenic_Gm24202|Gm8100_chr8_27938930_R | GTCTCGTGGGCTCGGGATCTCTCCTCTCCTCTGCG | 58.7 | 0.33 |
| mm4_intergenic_Plekhb2|Gm25096_chr1_35115649_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Plekhb2|Gm25096_chr1_35115649_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_1810013L24Rik|Gm22224_chr16_8862841_F | TCGTCGGCAGCGTCCTAGGGCTTCAGGTCTGTGC | 60.1 | 0.30 |
| mm4_intergenic_1810013L24Rik|Gm22224_chr16_8862841_R | GTCTCGTGGGCTCGGATGCCGAGAAAAGCAAGGGA | 59.9 | 0.30 |
| mm4_intergenic_Gm17131|Tmem132d_chr5_127887879_F | TCGTCGGCAGCGTCTCCTGAGAAAGCCCCAAACA | 59.1 | 0.30 |
| mm4_intergenic_Gm17131|Tmem132d_chr5_127887879_R | GTCTCGTGGGCTCGGGAGTGGCTGGATCTGTGGAG | 59.8 | 0.30 |
| mm4_exon_Gm17230_chr10_20626610_F | TCGTCGGCAGCGTCGCCATTTTGGGGAGGACATG | 59.1 | 0.29 |
| mm4_exon_Gm17230_chr10_20626610_R | GTCTCGTGGGCTCGGAGGCCAGCATCATCGTAAACT | 59.7 | 0.29 |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10796220_F | TCGTCGGCAGCGTCCGACCAAACCACAGTGACCT | 60.1 | 0.27 |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10796220_R | GTCTCGTGGGCTCGGATGCGTGACACCAACTCACT | 59.8 | 0.27 |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10794089_F | TCGTCGGCAGCGTCATGCGTGACACCAACTCACT | 59.8 | 0.27 |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10794089_R | GTCTCGTGGGCTCGGGACCAGAGACACAGCCTCAC | 60.0 | 0.27 |
| mm4_intron_Cacna1a_chr8_84595288_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Cacna1a_chr8_84595288_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53889848_F | TCGTCGGCAGCGTCTTTACTCGCCTTCAGCCCTG | 60.0 | 0.23 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53889848_R | GTCTCGTGGGCTCGGACAGCACTCTCTGTACGCAG | 59.7 | 0.23 |
| mm3_intergenic_Ugcg|Gm12596_chr4_59224466_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intergenic_Ugcg|Gm12596_chr4_59224466_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Slc22a27|Slc22a28_chr19_8038044_F | TCGTCGGCAGCGTCCTGTATCAGTGGCAGGGTCC | 59.8 | 0.21 |
| mm4_intergenic_Slc22a27|Slc22a28_chr19_8038044_R | GTCTCGTGGGCTCGGTCCTTGGCATTCCCTGTGTT | 59.5 | 0.21 |
| mm4_intergenic_Gm26475|Gm24522_chrX_128529652_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm26475|Gm24522_chrX_128529652_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intergenic_Olfr459|Pip_chr6_41790091_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Olfr459|Pip_chr6_41790091_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Siah3|Spert_chr14_75545094_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Siah3|Spert_chr14_75545094_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Dnajc6|Gm22533_chr4_101551659_F | TCGTCGGCAGCGTCCGGCTCTACCTTTGTGGCTT | 60.3 | 0.19 |
| mm4_intergenic_Dnajc6|Gm22533_chr4_101551659_R | GTCTCGTGGGCTCGGTCCTACTTGGTTTCATCCCAACA | 59.6 | 0.19 |
| mm4_intergenic_Cntnap4|Gm25766_chr8_113482107_F | TCGTCGGCAGCGTCGTGGAGATGCATTTGCTCGC | 60.2 | 0.19 |
| mm4_intergenic_Cntnap4|Gm25766_chr8_113482107_R | GTCTCGTGGGCTCGGTCGGAGCTGACCTGACACTA | 59.9 | 0.19 |
| mm4_intron_Amz1_chr5_140742014_F | TCGTCGGCAGCGTCTGGCTTGAAGGAGATGGTCTG | 59.7 | 0.19 |
| mm4_intron_Amz1_chr5_140742014_R | GTCTCGTGGGCTCGGATGGCCAGGAATGAGAGCAC | 60.1 | 0.19 |
| mm4_intergenic_Adcy2|Gm26844_chr13_69034393_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Adcy2|Gm26844_chr13_69034393_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_2900026A02Rik_chr5_113101716_F | TCGTCGGCAGCGTCGAGACCAACACCCCAGTCTG | 59.9 | 0.18 |
| mm4_intron_2900026A02Rik_chr5_113101716_R | GTCTCGTGGGCTCGGATACCGGAACCAAGCCACAG | 60.0 | 0.18 |
| mm4_intron_Smyd1_chr6_71261640_F | TCGTCGGCAGCGTCGCACGGTCTCTTAGCCAAGT | 60.0 | 0.17 |
| mm4_intron_Smyd1_chr6_71261640_R | GTCTCGTGGGCTCGGCTGCTGGCATCTCTTTTCTGC | 59.8 | 0.17 |
| mm4_intergenic_Gm25800|Efna5_chr17_62494981_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25800|Efna5_chr17_62494981_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25781|Ipo5_chr14_120846440_F | TCGTCGGCAGCGTCGGTTCAGTTGCTTTGCTGGG | 59.9 | 0.15 |
| mm4_intergenic_Gm25781|Ipo5_chr14_120846440_R | GTCTCGTGGGCTCGGAGGACTGCTAGGGGAGGAAG | 60.0 | 0.15 |
| mm4_exon_Col16a1_chr4_130068379_F | TCGTCGGCAGCGTCACCCATTCTGACTTGCAGGG | 59.9 | 0.15 |
| mm4_exon_Col16a1_chr4_130068379_R | GTCTCGTGGGCTCGGCACCTCCAGGCCATAGGAGA | 60.3 | 0.15 |
| mm4_intron_Hivep3_chr4_119854933_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Hivep3_chr4_119854933_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Fgf11_chr11_69799393_F | TCGTCGGCAGCGTCATAGCCCCTCAGCGTTCATG | 59.8 | 0.15 |
| mm4_exon_Fgf11_chr11_69799393_R | GTCTCGTGGGCTCGGGATTCCTGTGGGACTCCGTG | 60.1 | 0.15 |
| mm4_intron_Trim27_chr13_21179670_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Trim27_chr13_21179670_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Pigc|Dnm3_chr1_162036003_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Pigc|Dnm3_chr1_162036003_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intron_Trio_chr15_27854718_F | TCGTCGGCAGCGTCTGCCCCTGGTACTCCATACA | 59.9 | 0.13 |
| mm3_intron_Trio_chr15_27854718_R | GTCTCGTGGGCTCGGACACTTCCTTGGCCATTGGG | 60.5 | 0.13 |
| mm4_intron_Fam19a4_chr6_96955268_F | TCGTCGGCAGCGTCCCATACAGTCAGCATGGGCA | 60.1 | 0.13 |
| mm4_intron_Fam19a4_chr6_96955268_R | GTCTCGTGGGCTCGGGCTCTAGTAGGCACCTTGGG | 59.5 | 0.13 |
| mm4_exon_Gm12601_chr4_88614002_F | TCGTCGGCAGCGTCAAGCATGGGTGGGGTTTTGA | 60.1 | 0.12 |
| mm4_exon_Gm12601_chr4_88614002_R | GTCTCGTGGGCTCGGAGTGGGGAAAGAAGCTAGATGA | 58.5 | 0.12 |
| mm3_intergenic_Gm4944|Zfp944_chr17_22249463_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Gm4944|Zfp944_chr17_22249463_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Spata18|Usp46_chr5_73764457_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Spata18|Usp46_chr5_73764457_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22959|Daam1_chr12_71819529_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm22959|Daam1_chr12_71819529_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Rp1l1|Prss55_chr14_64058520_F | TCGTCGGCAGCGTCACAGGGTGGTAGGCTACACT | 59.8 | 0.10 |
| mm4_intergenic_Rp1l1|Prss55_chr14_64058520_R | GTCTCGTGGGCTCGGCACCTAGCGCTGATCACCAT | 59.8 | 0.10 |
| mm4_intron_Sdk2_chr11_113782316_F | TCGTCGGCAGCGTCTGTCTTGCTTTCCTCGTCCC | 59.9 | 0.10 |
| mm4_intron_Sdk2_chr11_113782316_R | GTCTCGTGGGCTCGGGCTGGATTTTGTTGCCAGCA | 59.9 | 0.10 |
| mm4_exon_Gm5148_chr3_37724319_F | TCGTCGGCAGCGTCACGTGACTGTGTTGGCTAGG | 59.9 | 0.10 |
| mm4_exon_Gm5148_chr3_37724319_R | GTCTCGTGGGCTCGGTTGTCCCGATCAAAGCAGCA | 60.2 | 0.10 |
| mm3_intron_Ccdc169_chr3_55153951_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intron_Ccdc169_chr3_55153951_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Snx18_chr13_113595066_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Snx18_chr13_113595066_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm1604b|Rps6ka2_chr17_7170050_F | TCGTCGGCAGCGTCAAGGGGAAAAGAGCTCTGGC | 59.9 | 0.10 |
| mm4_intergenic_Gm1604b|Rps6ka2_chr17_7170050_R | GTCTCGTGGGCTCGGGCCCATAGACTACACCCAGC | 59.8 | 0.10 |
| mm3_exon_Fam179a_chr17_71714375_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_exon_Fam179a_chr17_71714375_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Gm22444|mmu-mir-6238_chr7_53332766_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Gm22444|mmu-mir-6238_chr7_53332766_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Gbf1_chr19_46259778_F | TCGTCGGCAGCGTCCCAAGGGAGATCACCACCAG | 59.7 | 0.09 |
| mm4_exon_Gbf1_chr19_46259778_R | GTCTCGTGGGCTCGGCCAGGCTCAGGTTCCTACAG | 59.4 | 0.09 |
| mm4_intron_Zfp618_chr4_63079635_F | TCGTCGGCAGCGTCTCCCAGTATCCAGAGGTGCC | 60.6 | 0.09 |
| mm4_intron_Zfp618_chr4_63079635_R | GTCTCGTGGGCTCGGGCAGCCCCATTTTACATCTGG | 59.5 | 0.09 |
| mm4_intron_Baiap2l1_chr5_144349091_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Baiap2l1_chr5_144349091_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Elk3_chr10_93280998_F | TCGTCGGCAGCGTCATTGCCAGCCTAATGTCGGT | 59.7 | 0.09 |
| mm4_intron_Elk3_chr10_93280998_R | GTCTCGTGGGCTCGGTTTTCTGGCCAACGCTTCCA | 60.7 | 0.09 |
| mm4_intron_Cers6_chr2_69097874_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Cers6_chr2_69097874_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm12600|Gm23154_chr4_87419290_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm12600|Gm23154_chr4_87419290_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Zfp943|Zfp947_chr17_22093661_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Zfp943|Zfp947_chr17_22093661_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm25653|Gm11251_chr4_72938189_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm25653|Gm11251_chr4_72938189_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm22308|Cbfa2t2-ps1_chr5_59407849_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm22308|Cbfa2t2-ps1_chr5_59407849_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Atp6v1h|Oprk1_chr1_5327770_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Atp6v1h|Oprk1_chr1_5327770_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ugt1a6a_chr1_88137513_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ugt1a6a_chr1_88137513_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Pga5|Vps37c_chr19_10684738_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Pga5|Vps37c_chr19_10684738_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Igsf21_chr4_140159194_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Igsf21_chr4_140159194_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_4933440J02Rik|Krr1_chr10_111827379_F | TCGTCGGCAGCGTCGAGGTGCCATCTGCCTCTTT | 60.0 | 0.07 |
| mm4_intergenic_4933440J02Rik|Krr1_chr10_111827379_R | GTCTCGTGGGCTCGGCTGGAGTGTGTCTCAGCCAG | 60.0 | 0.07 |
| mm3_intergenic_Gm13018|Prdm2_chr4_143029733_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm13018|Prdm2_chr4_143029733_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Tmem219|Kctd13_chr7_126927206_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tmem219|Kctd13_chr7_126927206_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Frmd4a_chr2_4566799_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Frmd4a_chr2_4566799_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sirt2_chr7_28780440_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sirt2_chr7_28780440_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ak5_chr3_152489000_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ak5_chr3_152489000_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190250573_F | TCGTCGGCAGCGTCCACAGGGTCAGCATGGCTC | 60.7 | 0.06 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190250573_R | GTCTCGTGGGCTCGGAACTTGAACTTGCTGCAGCG | 59.9 | 0.06 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190250497_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190250497_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Gm24388|Gm5205_chr14_40447229_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm24388|Gm5205_chr14_40447229_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Sec14l5|Nagpa_chr16_5186148_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Sec14l5|Nagpa_chr16_5186148_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Col2a1_chr15_97979159_F | TCGTCGGCAGCGTCTGGGTAGCAGGAGAAGCTCA | 60.2 | 0.05 |
| mm4_exon_Col2a1_chr15_97979159_R | GTCTCGTGGGCTCGGACACCGAGGTTTCACTGGAC | 59.8 | 0.05 |
| mm4_intron_Ttc24_chr3_88072920_F | TCGTCGGCAGCGTCCCACAGACCCTTCAGGCATT | 59.9 | 0.05 |
| mm4_intron_Ttc24_chr3_88072920_R | GTCTCGTGGGCTCGGGCTGCCCAAGACACTGGTAA | 60.2 | 0.05 |
| mm4_intergenic_Gm26018|BC018507_chr13_70221629_F | TCGTCGGCAGCGTCGCGCCAACCCATATTCGAGA | 60.5 | 0.05 |
| mm4_intergenic_Gm26018|BC018507_chr13_70221629_R | GTCTCGTGGGCTCGGGCAGCGGTGTTTGTTACTGT | 59.3 | 0.05 |
| mm4_intergenic_Pdgfd|Ddi1_chr9_6169309_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Pdgfd|Ddi1_chr9_6169309_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gpr115|Gpr111_chr17_42697519_F | TCGTCGGCAGCGTCCGCCCATATGAGGAGTGCAT | 59.9 | 0.05 |
| mm4_intergenic_Gpr115|Gpr111_chr17_42697519_R | GTCTCGTGGGCTCGGTGCAGACTGACTGGTAATGGT | 59.0 | 0.05 |
| mm4_intergenic_U6|Mylk4_chr13_32762051_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_U6|Mylk4_chr13_32762051_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm26006|Gm26139_chr14_98602544_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm26006|Gm26139_chr14_98602544_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Fndc3c1|Cysltr1_chrX_106511009_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Fndc3c1|Cysltr1_chrX_106511009_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gm10863_chr15_79179100_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gm10863_chr15_79179100_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Nxph2_chr2_23367553_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Nxph2_chr2_23367553_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_1500015L24Rik|Gm6684_chr19_20561204_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_1500015L24Rik|Gm6684_chr19_20561204_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Fchsd1_chr18_37963105_F | TCGTCGGCAGCGTCCACTCCACAAGTCCTCCCAC | 59.9 | 0.04 |
| mm4_exon_Fchsd1_chr18_37963105_R | GTCTCGTGGGCTCGGGAGCTGACCATCACAGAGGG | 59.8 | 0.04 |
| mm4_intergenic_Lims1|Gm23058_chr10_58333793_F | TCGTCGGCAGCGTCAGACATGCTTTCCTCCAGCC | 60.0 | 0.04 |
| mm4_intergenic_Lims1|Gm23058_chr10_58333793_R | GTCTCGTGGGCTCGGCCAGACACAGGACTGACCAC | 59.9 | 0.04 |
| mm4_intron_Npepl1_chr2_174117856_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Npepl1_chr2_174117856_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Bcl7c_chr7_127666219_F | TCGTCGGCAGCGTCCTGAAAGGTCACTGGGCCAT | 59.9 | 0.04 |
| mm4_exon_Bcl7c_chr7_127666219_R | GTCTCGTGGGCTCGGTCCACGAGCCTATGGACTCT | 59.7 | 0.04 |
| mm3_intergenic_Megf9|Gm11227_chr4_70773365_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Megf9|Gm11227_chr4_70773365_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Il1rapl2|Gm22057_chrX_138239124_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Il1rapl2|Gm22057_chrX_138239124_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Nox3|Gm23186_chr17_4138372_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nox3|Gm23186_chr17_4138372_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Pura|Cystm1_chr18_36325709_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Pura|Cystm1_chr18_36325709_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25094|Gm22312_chr6_93402773_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25094|Gm22312_chr6_93402773_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_4921524L21Rik|Rab18_chr18_6690416_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_4921524L21Rik|Rab18_chr18_6690416_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Slc9a9_chr9_95120601_F | TCGTCGGCAGCGTCAAGGCCCCAAAGTGGTACAG | 59.8 | 0.03 |
| mm4_intron_Slc9a9_chr9_95120601_R | GTCTCGTGGGCTCGGTCAACAAGCAAAGGTCCAACC | 59.2 | 0.03 |
| mm4_exon_Bace2_chr16_97438312_F | TCGTCGGCAGCGTCACCACTCCTGTGCCAAGTTC | 60.1 | 0.03 |
| mm4_exon_Bace2_chr16_97438312_R | GTCTCGTGGGCTCGGCACCAAAGACCCTCCTGTCA | 59.2 | 0.03 |
| mm4_intron_Tek_chr4_94850554_F | TCGTCGGCAGCGTCTGGACTGCAGCTTTCTGAGA | 58.9 | 0.02 |
| mm4_intron_Tek_chr4_94850554_R | GTCTCGTGGGCTCGGTTACACCTGATGCTGGGCTC | 59.7 | 0.02 |
| mm4_intergenic_Cldn14|Sim2_chr16_94019163_F | TCGTCGGCAGCGTCTTTTCTGGGATGCTCTGGGC | 60.3 | 0.02 |
| mm4_intergenic_Cldn14|Sim2_chr16_94019163_R | GTCTCGTGGGCTCGGTGCCTGGCCCTTTAAACCAA | 60.1 | 0.02 |
| mm4_exon_Cep78_chr19_15959673_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Cep78_chr19_15959673_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22146632_F | TCGTCGGCAGCGTCGAATGGTGGAGCTCAAGGGG | 60.3 | 0.02 |
| mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22146632_R | GTCTCGTGGGCTCGGTACATGCTTCTGAGGCGACC | 59.8 | 0.02 |
| mm4_intergenic_1700028N14Rik/Map3k14|Arhgap27_chr11_103289076_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_1700028N14Rik/Map3k14|Arhgap27_chr11_103289076_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12139|Gm12140_chr11_41258290_F | TCGTCGGCAGCGTCTGGTGGAAGGTTTGTTGTGGT | 60.2 | 0.02 |
| mm4_intergenic_Gm12139|Gm12140_chr11_41258290_R | GTCTCGTGGGCTCGGAGAGAAGAAAATCGGGAGTCTGA | 58.9 | 0.02 |
| mm4_intergenic_Gm11591|Gm11587_chr1_61221978_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm11591|Gm11587_chr1_61221978_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Pde4dip_chr3_97693429_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Pde4dip_chr3_97693429_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Adamtsl2_chr2_27092678_F | TCGTCGGCAGCGTCCTGCTGCAGCCTAACTCTGT | 60.0 | 0.01 |
| mm4_intron_Adamtsl2_chr2_27092678_R | GTCTCGTGGGCTCGGGCAAATGACTGCAGGTGTGG | 60.0 | 0.01 |
| mm4_intergenic_Gm13852|Chchd3_chr6_32650832_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13852|Chchd3_chr6_32650832_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ptprd_chr4_77586817_F | TCGTCGGCAGCGTCATGGTGAAGCACAGCTCTGT | 59.6 | 0.01 |
| mm4_intron_Ptprd_chr4_77586817_R | GTCTCGTGGGCTCGGGACAACTGGTGGCTGAGTCA | 59.8 | 0.01 |
| mm3_intergenic_Gm26514|Slc35b3_chr13_38954079_F | TCGTCGGCAGCGTCTGCACGTTTAGCAAGCTGAC | 59.4 | 0.01 |
| mm3_intergenic_Gm26514|Slc35b3_chr13_38954079_R | GTCTCGTGGGCTCGGTGGCCTAATCGAGCTACAGC | 59.6 | 0.01 |
| mm4_intergenic_Gm12518|Gm26144_chr4_56473500_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12518|Gm26144_chr4_56473500_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_6330403A02Rik|Parp1_chr1_180554225_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_6330403A02Rik|Parp1_chr1_180554225_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_30280440_F | TCGTCGGCAGCGTCGGCAGTAGAGGAAAAGCCCT | 59.3 | 0.01 |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_30280440_R | GTCTCGTGGGCTCGGCAGGTTCCATGTGTGCCTCT | 59.9 | 0.01 |
| mm4_intron_Rhpn1_chr15_75712483_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rhpn1_chr15_75712483_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hspa9|Ctnna1_chr18_35069324_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hspa9|Ctnna1_chr18_35069324_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AC112986.1|Tsn_chr1_118236564_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AC112986.1|Tsn_chr1_118236564_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rpap3_chr15_97684346_F | TCGTCGGCAGCGTCACAGACAACTCCTATCTCTCAGTG | 59.2 | 0.00 |
| mm4_intron_Rpap3_chr15_97684346_R | GTCTCGTGGGCTCGGTCTGTAAGTCAAGGGCGCAG | 60.0 | 0.00 |
| mm4_intergenic_Pcdh18|Slc7a11_chr3_50082790_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pcdh18|Slc7a11_chr3_50082790_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_H1f0|Gcat_chr15_79030627_F | TCGTCGGCAGCGTCGCGCCAGAGAATAGCAAAGC | 59.9 | 0.00 |
| mm4_intergenic_H1f0|Gcat_chr15_79030627_R | GTCTCGTGGGCTCGGGACTTCAACGCCTCTCCTCC | 60.1 | 0.00 |
| mm4_exon_Papd7_chr13_69506893_F | TCGTCGGCAGCGTCTTCCCACTGAGTTCTGCTGG | 59.6 | 0.00 |
| mm4_exon_Papd7_chr13_69506893_R | GTCTCGTGGGCTCGGCTGTGCATTGAGGACCCCTT | 59.9 | 0.00 |
| mm4_intron_Immp2l_chr12_41024172_F | TCGTCGGCAGCGTCCTTCCCCTGGGTTGAGCTG | 60.0 | 0.00 |
| mm4_intron_Immp2l_chr12_41024172_R | GTCTCGTGGGCTCGGCGGAGGAGAAGCCGGAGATA | 60.5 | 0.00 |
| mm4_intron_Zbtb7c_chr18_75933427_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zbtb7c_chr18_75933427_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tor3a_chr1_156666900_F | TCGTCGGCAGCGTCCTGTTTCCTAGGCCCTGGTG | 60.0 | 0.00 |
| mm4_intron_Tor3a_chr1_156666900_R | GTCTCGTGGGCTCGGCCAAGACCAGGGAGCATCAC | 60.3 | 0.00 |
| mm4_intron_Steap1_chr5_5749022_F | TCGTCGGCAGCGTCTCATCCAGCGAGGCAAACTT | 59.9 | 0.00 |
| mm4_intron_Steap1_chr5_5749022_R | GTCTCGTGGGCTCGGGAGCAGGTCTCCGCGGTC | 62.5 | 0.00 |
| mm4_intron_Rorc_chr3_94388396_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rorc_chr3_94388396_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168327867_F | TCGTCGGCAGCGTCGGAGTGCCTCTCTCATCTGC | 59.8 | 0.00 |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168327867_R | GTCTCGTGGGCTCGGCATTTCGCCTGGCAGAATCC | 59.6 | 0.00 |
| mm4_intergenic_Gm23896|Gm24902_chr5_57043863_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23896|Gm24902_chr5_57043863_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122773287_F | TCGTCGGCAGCGTCCTTTCCCTTCCCGAAACCTCA | 59.9 | 0.00 |
| mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122773287_R | GTCTCGTGGGCTCGGACAAACAGCCGAGGTGGAAA | 60.1 | 0.00 |
| mm4_intergenic_Fdx1|Gm6981_chr9_51992357_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fdx1|Gm6981_chr9_51992357_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Rnf31_chr14_55594444_F | TCGTCGGCAGCGTCGTAACCAAGTCTCGTGCCCA | 59.9 | 0.00 |
| mm4_exon_Rnf31_chr14_55594444_R | GTCTCGTGGGCTCGGTCCCCAAGAAGAGAAAGCCTC | 59.3 | 0.00 |
| mm2_intron_Ebf4_chr2_130333493_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intron_Ebf4_chr2_130333493_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Gm23821|Tgfb2_chr1_186482325 |
TAGGCTGCTCTGTACACCCAGCCCCCAAGCTCAAGGTCAGCCTGACACTCTGAATGGTTAAATCATCCTTGGCAAAGAGT CTAAACTTGGCAGAAGCTCCGT |
| mm4_intergenic_Slc22a29|Slc22a30_chr19_8323154 |
GCTGCCAGAGTCGCATTTTCAGTTTCACAATCACTTGCTTTTTTATCCTCATCGCCATTTTCTGTATCAGTGGCGGGGTC CTCCTCTGATGAGGAAGACACTTTGGCACTCTGGTGGGTTAACACAGGGAATGCCAAGGA |
| mm4_intron_Tbc1d32_chr10_56128840 | TGGTAACATGCTTTCCTGATTGTAGAGGGTTAGCTTTGCATTCTGATAGGTAAAGGAACTGCTACATGTCTTGAACC |
| mm4_intergenic_Gm24202|Gm8100_chr8_27938930 |
AGCTCAGCGTGCCTCTTTAATCTCATGTGTTACTTATTAAAAAACATTCCTCTTACCTTTGTATCGCAGCTAGGGCTTCT CCCTGCATCTAATCTCCCACCAGAGTGCAGAGCTGCCTCGCAGAGGAGAGGAGAGATC |
| mm4_intergenic_1810013L24Rik|Gm22224_chr16_8862841 |
CTAGGGCTTCAGGTCTGTGCATGCTCAGCCTGGCGCTCTTGTGGGTATGCTCTGTCTACTGAGCATTGCTCCCTTGCTTT TCTCGGCAT |
| mm4_intergenic_Gm17131|Tmem132d_chr5_127887879 |
TCCTGAGAAAGCCCCAAACATTGGAATCATCACTCATTTTCTCTTGCTTCTGCCAGATTTACCCATCAAGAGTGTCATGC TGACCCCCTCTCATTGCCACCATGCTCCACAGATCCAGCCACTC |
| mm4_exon_Gm17230_chr10_20626610 |
GCCATTTTGGGGAGGACATGTTCATTTCAAAGCCCCCAAACCTATTCAAGTTCATCCCAGAGATAAGTACATATTTGGGT ACCTTCCTAGAAGAGTGCCAGGTTGACCGTAAGCAGCAGTTTACGATGATGCTGGCCT |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10796220 |
CGACCAAACCACAGTGACCTTTTCCTTCCAAGGTAGCAAGCTGCAGGCTTCTCAGCATGAGTGACAACTTTCATCCCTTG GAAATGGGCTTGGCACTCTGGTTGGAATTTCTTATAAGTGAGTTGGTGTCACGCAT |
| mm4_intergenic_4930556M19Rik|C1qtnf3_chr15_10794089 |
ATGCGTGACACCAACTCACTTATAAGAAATTCCAACCAGAGTGCCAAGCCCATTTCCAAGGGATGAAAACTGTCTGGACT AATCATGGGGGAATGAGGTGAGGCTGTGTCTCTGGTC |
| mm4_intergenic_Gm5449|Drd1a_chr13_53889848 |
TTTACTCGCCTTCAGCCCTGGTGGCTCACTGGCTCACGAGTGTGAATACAGGAGTGTGATTTCCCACCAGAGTGCAGAGC ATACCCTGGTGCTGCTGCGTACAGAGAGTGCTGT |
| mm4_intergenic_Slc22a27|Slc22a28_chr19_8038044 | CTGTATCAGTGGCAGGGTCCTCCTCTGATGAGGAAGCCACTTTGGCACTCTGGTGGGTTAACACAGGGAATGCCAAGGA |
| mm4_intergenic_Dnajc6|Gm22533_chr4_101551659 | CGGCTCTACCTTTGTGGCTTTAATCCTGGGGCAGCTAAGGACTCTGGTGGGAGTCATGTTGGGATGAAACCAAGTAGGA |
| mm4_intergenic_Cntnap4|Gm25766_chr8_113482107 |
GTGGAGATGCATTTGCTCGCGCATGCGGTGGGCTTGGCACTAAGGTAGGGCTAAAGCAAGAAGGCAATGATGAAGAATTA TCTCTGCTTATTTGCTTAGTGTCAGGTCAGCTCCGA |
| mm4_intron_Amz1_chr5_140742014 |
TGGCTTGAAGGAGATGGTCTGGGTCAGCTAGGTACTCAGATTGGCAGAGTGACTTGTCTTTTTAGCATCCTGCCCCCTAC TGGTCTATACTGGTTATATAGTGTGTGCTCTCATTCCTGGCCAT |
| mm4_intron_2900026A02Rik_chr5_113101716 |
GAGACCAACACCCCAGTCTGCTGAGACAGGACAGGAAGCAGCACTTTCTGGGCTCAGATGGGCACTCTGGATGGACTGTG GCTTGGTTCCGGTAT |
| mm4_intron_Smyd1_chr6_71261640 |
GCACGGTCTCTTAGCCAAGTTCTCTGTAATTAGAAAAAGTGTCTAAAGGCCCTGCTCAGCTTTGGACTCTGATAGGGATC ATAGATAGTTAGAGACAATGACTCCTTGCAGAAAAGAGATGCCAGCAG |
| mm4_intergenic_Gm25781|Ipo5_chr14_120846440 |
GGTTCAGTTGCTTTGCTGGGAAAGGGATGTGTCTATCTCAAGCCAAGCAGAGTCCCAGGCTAACCTGTCCCTAGGCTTCC CTGTCGCACAGGTTTTACTCCAGGATCTTCCTCCCCTAGCAGTCCT |
| mm4_exon_Col16a1_chr4_130068379 | ACCCATTCTGACTTGCAGGGGGAGCCTGGAACCCAGGGTTTGCCTGGCACTCAGGTAGGTCTCCTATGGCCTGGAGGTG |
| mm4_exon_Fgf11_chr11_69799393 |
ATAGCCCCTCAGCGTTCATGGCCATGTAGTGACCCAGCTTGGCACTCTGGATGGTGACCACACGGAGTCCCACAGGAATC |
| mm3_intron_Trio_chr15_27854718 |
TGCCCCTGGTACTCCATACACTCTGTCCTTGCTGCCTGCACTGTCCCCCTGGTCTTCTCCCTGGCCCCCTGCAGTCTCCC ACCCAGAGTGCAGAGCTGACCACCGGGCCTCCCCAATGGCCAAGGAAGTGT |
| mm4_intron_Fam19a4_chr6_96955268 |
CCATACAGTCAGCATGGGCAGAAGTAGGATCATTCCTCTTGCCAACCAGATTCTAGCAGCCAACCAGATTGCCAATCTGC CAACCAGATTCTACCCAAGGTGCCTACTAGAGC |
| mm4_exon_Gm12601_chr4_88614002 |
AAGCATGGGTGGGGTTTTGATGCTCATCATGGCAATCTGGTAGGTAGATGCTATTGACATAGCCAGAGAAAGACATTATT TCAGGGAAAATACTTTTCATCTAGCTTCTTTCCCCACT |
| mm4_intergenic_Rp1l1|Prss55_chr14_64058520 |
ACAGGGTGGTAGGCTACACTCATGCTGGTTCCCACCTCTCCCTGCTATATGACACCAGGTCAACTTGACTATCTGGTTGG GATGAACATTTGAGGTAGAGGCCTCTCATGCTGATTTTTGATGGTGATCAGCGCTAGGTG |
| mm4_intron_Sdk2_chr11_113782316 |
TGTCTTGCTTTCCTCGTCCCTGCCCACCAGTGTCCCTAGCTGGCCAATTCACCACTTATATGAACCTAAGGAAGACATGT TTGTCAAGGAGAGTGCTGGCAACAAAATCCAGC |
| mm4_exon_Gm5148_chr3_37724319 |
ACGTGACTGTGTTGGCTAGGGCGCACCTTGCTCCAGCAGGGACAGGTGAGCTCTGCACCCTGGTGGGAGCATGCATTTTG CAAACTCAGGTCTGCACCGCCCCACTGCTGCTTTGATCGGGACAA |
| mm4_intergenic_Gm1604b|Rps6ka2_chr17_7170050 |
AAGGGGAAAAGAGCTCTGGCTGCGGGCACTGATTGGTGGGTGGGCGTGTAGGCGTGGCGCAGCGTCGCACTCTGGTTGGT CGGAACGACGCTGGGTGTAGTCTATGGGC |
| mm4_exon_Gbf1_chr19_46259778 |
CCAAGGGAGATCACCACCAGTGAGCCTGGGAGCAGTGAGATGGGCGCTTCTGATCAGCTTGACCCTCAGGTAGGACTGAC TCCTGAAGGCCCCTCTTCAGATCTACATGTCACTGTAGGAACCTGAGCCTGG |
| mm4_intron_Zfp618_chr4_63079635 |
TCCCAGTATCCAGAGGTGCCTTTTAAGGTCACTTGTAACTCCAATTGTGAAAGATACAAACAAATGACAGGAGGTGAGCT GGGTACTCTGGAAGGCTTTAGACCTTCACTTACCCAGATGTAAAATGGGGCTGC |
| mm4_intron_Elk3_chr10_93280998 |
ATTGCCAGCCTAATGTCGGTAGAGTTAAACTCGTTTAAACCAGTTTGACCGATGCAGCTCACCAGAGTGCAAGGCTGGCA AGGCTGGCAAAGCTGGAAGCGTTGGCCAGAAAA |
| mm4_intergenic_4933440J02Rik|Krr1_chr10_111827379 |
GAGGTGCCATCTGCCTCTTTACGTCTGCTGCAGAAAATTCTTTGCAGAGCTGAGATTCCTAAGTCACCCTCTCCAAAACC TCCCAGAGTGCTCAGCTGTCCCCTCCCCTGGCTGAGACACACTCCAG |
| mm4_intergenic_Gm17566|Gm23153_chr1_190250573 |
CACAGGGTCAGCATGGCTCGCTGCAGGGCCCACCTACCCACAGGGTCAGCATGGCTCTCTGCAGGGCCCACCTACCTGCC TGGCATGCACAGTGCGCTGCAGCAAGTTCAAGTT |
| mm4_exon_Col2a1_chr15_97979159 |
TGGGTAGCAGGAGAAGCTCAGGGGGTCTGAAAAGTAGGAGGTCTTGGGCAGCCCCCTCATGGACAAATGACATGCAGTGA CACCCACCGGAGGGCCAGGCAGACCCTGCAGTCCAGTGAAACCTCGGTGT |
| mm4_intron_Ttc24_chr3_88072920 |
CCACAGACCCTTCAGGCATTGTGTACCCTCGGTCTTTGTGTCCCCCTCCCTGAGTTCCAAGCTAACCCTCTCATTCTTAC CAGTGTCTTGGGCAGC |
| mm4_intergenic_Gm26018|BC018507_chr13_70221629 |
GCGCCAACCCATATTCGAGACTCTCATAACTTATTTAAATATTCCCAAAGCATCTGAAGAAAGATATGTCTGGGTGAATT ATTCCTATGGAGCTGCCAACCAGGGTGACAAGCTCACATTACAGTAACAAACACCGCTGC |
| mm4_intergenic_Gpr115|Gpr111_chr17_42697519 |
CGCCCATATGAGGAGTGCATCCCAATTTGTTAGCTTGGCCCTTTGGTTGGATGACAAGAGAATTCAGAAGAAAAGTCCTG TTGGAGACAAAATATAGCTGCATTCACCATTACCAGTCAGTCTGCA |
| mm4_exon_Fchsd1_chr18_37963105 |
CACTCCACAAGTCCTCCCACTGCCCCCAACCAGGGTCCCATGCTCACCTTAACCCACTCGTCAGCATCTCCTTCCTCTAT GACCTCCAGCCACTCACCCTCTGTGATGGTCAGCTC |
| mm4_intergenic_Lims1|Gm23058_chr10_58333793 |
AGACATGCTTTCCTCCAGCCATCTCCAGTTAGCCTACTTGATAGAGAATCGACACCGCCACACAGTGAGGTCTGAGGTTA TCTTGCCCCTCTGGTCGGGTGGTCAGTCCTGTGTCTGG |
| mm4_exon_Bcl7c_chr7_127666219 |
CTGAAAGGTCACTGGGCCATGTGCACCCCTCGATTGACCTGAGGCAAGGTTGGCACCCTGGTTGGCACATGTCTTCTGGA GCCAAGGGTTAATTAGCAGGCCCAGAGTCCATAGGCTCGTGGA |
| mm4_intron_Slc9a9_chr9_95120601 |
AAGGCCCCAAAGTGGTACAGCAATTGATGCTTGGGCATCTCCGCATCTCCATTCAATCTGCAGCCCACCAGGGTGCCCAG CAGCCCTGAGACAGGTTGGACCTTTGCTTGTTGA |
| mm4_exon_Bace2_chr16_97438312 |
ACCACTCCTGTGCCAAGTTCAGTGTTCACTTTGTGATGCTACCTGTTACCAGGATAGGTTGGACAAAAAGCATTCCACCC AGAGTGCTAAGCAGCCCCTCTGACAGGAGGGTCTTTGGTG |
| mm4_intron_Tek_chr4_94850554 |
TGGACTGCAGCTTTCTGAGATAGTTTGAGGTCCTCCCAGAGTGCCAAGCACACTATGCTCACTGCTTGGTGAAATCTGTC AAACATAATTGCATGCTCTGGAGCCCAGCATCAGGTGTAA |
| mm4_intergenic_Cldn14|Sim2_chr16_94019163 |
TTTTCTGGGATGCTCTGGGCCCTGGTTTGTTCAGCTCTGAGCGCCCGAGTCAGCTTGGCTCTGTAGTTGGGGACTCACTC CAGAGCCTTTTTCTTACTTCACTGTCTCTCTTGGTTTAAAGGGCCAGGCA |
| mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22146632 |
GAATGGTGGAGCTCAAGGGGGTGGTCCAGGGGAGTTGAGTCATGAGTCAGCTAGGCCCTGTGGTTGGAGTGGGAATCTGG ATGAGATTTTCACCCATGTCTTTGATTACTGAGGGTCGCCTCAGAAGCATGTA |
| mm4_intergenic_Gm12139|Gm12140_chr11_41258290 |
TGGTGGAAGGTTTGTTGTGGTTTGCATAATAGGTCAGCTTGCTACTGTAGTGGGCATCAGATAGATTAGCAAATCTGCAA TTTGGGACTGAAAAGATTGATCAGACTCCCGATTTTCTTCTCT |
| mm4_intron_Adamtsl2_chr2_27092678 |
CTGCTGCAGCCTAACTCTGTCTGGTGGAGCAGACCTAGCAGGGTGAGAAGCTGACCCAGGCTCATTCTTCTCTTCTGCCT TCTCTGTCCTGAAGCTTTAAGCATCAGAGTTTCTTGACCACACCTGCAGTCATTTGC |
| mm4_intron_Ptprd_chr4_77586817 |
ATGGTGAAGCACAGCTCTGTGAATCCTGTAATTGAGTCAGTCCTGCCAAAGTGCTAAGCTGAGCCATGAAAACGCTGGCT TCCTAAGCCTAACAAGGCTTTTTGATTGACTCAGCCACCAGTTGTC |
| mm3_intergenic_Gm26514|Slc35b3_chr13_38954079 |
TGCACGTTTAGCAAGCTGACTTAGTTTTTCCAGATTCAACTGATGAGAGTACCGCTCACTCCCCAGAGTGCCAAGCCCAC CACATACCTTCTCCTTCTGTCCTGAGTGAGCTGTAGCTCGATTAGGCCA |
| mm4_intergenic_Unc5d|4933433F19Rik_chr8_30280440 |
GGCAGTAGAGGAAAAGCCCTATCCTGTTCTCTAACTCCAAATGTTTTTGTGGCTTTCACTTCAGGGAAGGTGCCCACACA TGTGCCAAGCTGACCTCAACCTTGTCCTGTATAAGAGGCACACATGGAACCTG |
| mm4_intron_Rpap3_chr15_97684346 |
ACAGACAACTCCTATCTCTCAGTGAGGTCTGCTTGGGACCCTGGGAGGGGAGCAATAGTGAGAACCAGGTTTGTTAAGCT TTCACACGGACTTTCTCGGACCTCTTACTGCGCCCTTGACTTACAGA |
| mm4_intergenic_H1f0|Gcat_chr15_79030627 |
GCGCCAGAGAATAGCAAAGCTTTGGAGTGACAGGGACGCTCCAACAGGAGGGTCAGCTGGGCACGTAGGTGGGTCTTCGG GAGAGGTAGGTGTGTAGGCAAGGTCTCGGGAGGAGAGGCGTTGAAGTC |
| mm4_exon_Papd7_chr13_69506893 |
TTCCCACTGAGTTCTGCTGGTAGAGATAAGCCAGCACTGTGCTTCCAGCCAGAGGGGCAAGCTCACCAGGCAGTAAGGGG TCCTCAATGCACAG |
| mm4_intron_Immp2l_chr12_41024172 |
CTTCCCCTGGGTTGAGCTGCGCAGCGGGTGCGCGGGGAATGGGGCGGCGGGTCCGGGCGGCTTGGCACGCGGGTGGGCTT AAGCAGACCCTCTGTGTCCCCGGCAGGGTCTCTTATCTCCGGCTTCTCCTCCG |
| mm4_intron_Tor3a_chr1_156666900 |
CTGTTTCCTAGGCCCTGGTGCTGTGGACGACCCAGGCCTGGGCTCCACCCACCACAATCACCGAACCCGGCCTTGGCCCC ACCAGGCTGCCAAGCTCCCCAAGTGATGCTCCCTGGTCTTGG |
| mm4_intron_Steap1_chr5_5749022 |
TCATCCAGCGAGGCAAACTTGCGGAAACTGCCAAGTTGGCAAACTTACCGGGGGAACCCAACCTGACTGCAAAGTTGACC TAAGCTCCAGAGCCGGGACCGCGGAGACCTGCTC |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168327867 |
GGAGTGCCTCTCTCATCTGCGGGAATCGTAACACAGGGCCTTCCTACCAGACTCCCAAGCAGACGTGTGCTAAAATCTAA ACGGATTCTGCCAGGCGAAATG |
| mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122773287 |
CTTTCCCTTCCCGAAACCTCAAATCCTGCCCACCAGACTTCCCAGCAGACCCAGCCAATCTCTTCCCCCTCGATTTCCAC CTCGGCTGTTTGT |
| mm4_exon_Rnf31_chr14_55594444 |
GTAACCAAGTCTCGTGCCCAGCTTGTGACATTTTGTTCCATGGGCATCCGTCCCGTGCACATCACCTTCGCCAAGCCCTG CCTGGGTCCCACCAGACTGCCAGCCTGAGCTCTAGGTGAGAGGCTTTCTCTTCTTGGGGA |