CRISPOR

DNA break repair outcome predictions

Guide: CCACGCTCCCGGGAAGTCGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATC`	(Wild-type sequence)
402	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG--------CTCGCGGGCTCAGGCAACCGTG`	`8 bp deleted → frameshift`
359	`GCCTCCCCCGCCACGCTCCCGG----------------CTCGCGGGCTCAGGCAACCGTG`	`16 bp deleted → frameshift`
346	`GCCTCCCCCGCCACGCTCCCGGG-----------CCGGCTCGCGGGCTCAGGCAACCGTG`	`11 bp deleted → frameshift`
344	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG---GCCGGCTCGCGGGCTCAGGCAACCGTG`	`3 bp deleted → no frameshift`
327	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG----CCGGCTCGCGGGCTCAGGCAACCGTG`	`4 bp deleted → frameshift`
283	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG---------------GCTCAGGCAACCGTG`	`15 bp deleted → no frameshift`
261	`GCCTCCCCCGCCACGCTCCCGGGAAGTCG-------------CGGGCTCAGGCAACCGTG`	`13 bp deleted → frameshift`
253	`GCCTCCCCCGCCACGCTCCCGGG-----------------------CTCAGGCAACCGTG`	`23 bp deleted → frameshift`
242	`GCCTCCCCCGCCACGCTCCCGGG----------GCCGGCTCGCGGGCTCAGGCAACCGTG`	`10 bp deleted → frameshift`
222	`GCCTCCCCCGCCACGCTCCCGGGAAG------GGCCGGCTCGCGGGCTCAGGCAACCGTG`	`6 bp deleted → no frameshift`
221	`GCCTCCCCCGCCACGCTC-----------------------GCGGGCTCAGGCAACCGTG`	`23 bp deleted → frameshift`
219	`GCCTCCCCCGCCACGCTCCCGG------------CCGGCTCGCGGGCTCAGGCAACCGTG`	`12 bp deleted → no frameshift`
188	`GCCTCCCCCGCCACGCTCCCGGG---------------CTCGCGGGCTCAGGCAACCGTG`	`15 bp deleted → no frameshift`
180	`GCCTCCCCCGCC------------------------GGCTCGCGGGCTCAGGCAACCGTG`	`24 bp deleted → no frameshift`
179	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG----------------CTCAGGCAACCGTG`	`16 bp deleted → frameshift`
170	`GCCTCCCCCGCCACGCTCC-----------------GGCTCGCGGGCTCAGGCAACCGTG`	`17 bp deleted → frameshift`
155	`GCCTCCCCCGCCACGC---------------------------GGGCTCAGGCAACCGTG`	`27 bp deleted → no frameshift`
155	`GCCTCCCCCG---------------------------GCTCGCGGGCTCAGGCAACCGTG`	`27 bp deleted → no frameshift`
154	`GCCTCCCCCGCCACGC-------------------CGGCTCGCGGGCTCAGGCAACCGTG`	`19 bp deleted → frameshift`
148	`GCCTCCCCCGCCACGCTC-------------------------------AGGCAACCGTG`	`31 bp deleted → frameshift`
140	`GCCTCCCCCGCCACGCTCCCG---------------------CGGGCTCAGGCAACCGTG`	`21 bp deleted → no frameshift`
133	`GCCTCCCCCGCCACGCTCCCGGGAAGTCGG----------------------CAACCGTG`	`22 bp deleted → frameshift`
133	`GCCTCCCCCGCCACGCTCCCGGG----------------------GCTCAGGCAACCGTG`	`22 bp deleted → frameshift`
133	`GCCTCCCCCGCCACG----------------------GCTCGCGGGCTCAGGCAACCGTG`	`22 bp deleted → frameshift`
121	`GCCTCCCCCGC--------------------------------GGGCTCAGGCAACCGTG`	`32 bp deleted → frameshift`
120	`GCCTCCCCCGCCACGCTCCCGG------------------------CTCAGGCAACCGTG`	`24 bp deleted → no frameshift`
115	`GCC---------------------------------GGCTCGCGGGCTCAGGCAACCGTG`	`33 bp deleted → no frameshift`
105	`GCCTCCCCCGCCACGCTCCCGGGAAGTC---------------------AGGCAACCGTG`	`21 bp deleted → no frameshift`
98	`GCCTCCCCCGC----------------------------TCGCGGGCTCAGGCAACCGTG`	`28 bp deleted → frameshift`
98	`GCCTCCCC----------------------------GGCTCGCGGGCTCAGGCAACCGTG`	`28 bp deleted → frameshift`
94	`GCCTCCCCCGCCACGCTCCCG-------------------------------------TG`	`37 bp deleted → frameshift`
94	`GCCTCCCCCGCCACGCTCCCGGGAAGTCG-----------------------------TG`	`29 bp deleted → frameshift`
94	`GCCTCCCCCGCCACGCTCCCGGG-----------------------------CAACCGTG`	`29 bp deleted → frameshift`
94	`GCCTCCCCCGCCACG-----------------------------GGCTCAGGCAACCGTG`	`29 bp deleted → frameshift`
94	`GCCTCCC-----------------------------GGCTCGCGGGCTCAGGCAACCGTG`	`29 bp deleted → frameshift`
89	`GCCTCCCCCGCCACGCTCCCGG------------------------------CAACCGTG`	`30 bp deleted → no frameshift`
89	`GCCTCC------------------------------GGCTCGCGGGCTCAGGCAACCGTG`	`30 bp deleted → no frameshift`
86	`GCCTCCCCCGCCACGCTCCCGGGAAG-------------------------GCAACCGTG`	`25 bp deleted → frameshift`
82	`GCCTC------------------------------------GCGGGCTCAGGCAACCGTG`	`36 bp deleted → no frameshift`
73	`GCCTCCCCCG----------------------------------GGCTCAGGCAACCGTG`	`34 bp deleted → frameshift`
66	`GCCTCCCCCGC------------------------------------TCAGGCAACCGTG`	`36 bp deleted → no frameshift`
62	`GCCTCCCCCGCCACGC-------------------------------------AACCGTG`	`37 bp deleted → frameshift`
62	`GC-------------------------------------TCGCGGGCTCAGGCAACCGTG`	`37 bp deleted → frameshift`
60	`GCCTCCCCCGCCACGCTCCCGGGAAGT--------------------------------G`	`32 bp deleted → frameshift`
60	`GCCTCCCCCGCCACGCTCC--------------------------------------GTG`	`38 bp deleted → frameshift`
55	`GCCTC--------------------------------------------AGGCAACCGTG`	`44 bp deleted → frameshift`
54	`GCCTCCCCCG------------------------------------------------TG`	`48 bp deleted → no frameshift`
51	`GC-----------------------------------------GGGCTCAGGCAACCGTG`	`41 bp deleted → frameshift`
48	`GCCTCCCCCGC------------------------------------------AACCGTG`	`42 bp deleted → no frameshift`
47	`GCCTCCCCCGCCA-------------------------------------GGCAACCGTG`	`37 bp deleted → frameshift`
46	`GCCTCCCCCGCCACG-------------------------------------------TG`	`43 bp deleted → frameshift`
44	`GCCTCCCCCGCCACGCTCCCGGGAA------------------------------CCGTG`	`30 bp deleted → no frameshift`
42	`GCCTCCCCCGCC---------------------------------------------GTG`	`45 bp deleted → no frameshift`
42	`GC---------------------------------------------TCAGGCAACCGTG`	`45 bp deleted → no frameshift`
38	`GCCTCCCCCGCCA-----------------------------------------ACCGTG`	`41 bp deleted → frameshift`
36	`GCCTCCCCCGCCAC------------------------------------------CGTG`	`42 bp deleted → no frameshift`
34	`GCCTCCCC-------------------------------------------------GTG`	`49 bp deleted → frameshift`
32	`GCCTCCC--------------------------------------------------GTG`	`50 bp deleted → frameshift`
31	`GCCTCC---------------------------------------------------GTG`	`51 bp deleted → no frameshift`
31	`GC---------------------------------------------------AACCGTG`	`51 bp deleted → no frameshift`
26	`GCC------------------------------------------------------GTG`	`54 bp deleted → no frameshift`

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