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Contents:

PCR primers for off-targets of TTTCTGGATGGGGGTCAGCT TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313871_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313871_R Primer3: not found at this Tm N.d. 1.00
mm4_intergenic_Myh9|Gm22107_chr15_77824847_F TCGTCGGCAGCGTCCCCAAGACCTTGTCTCGCTT 59.9 0.63
mm4_intergenic_Myh9|Gm22107_chr15_77824847_R GTCTCGTGGGCTCGGCGTTACATTTCACCAGCACCA 59.1 0.63
mm3_intergenic_Gm23011|Gm26691_chr3_133690625_F Primer3: not found at this Tm N.d. 0.51
mm3_intergenic_Gm23011|Gm26691_chr3_133690625_R Primer3: not found at this Tm N.d. 0.51
mm4_intergenic_Cxxc4|Gm26820_chr3_134296743_F Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_Cxxc4|Gm26820_chr3_134296743_R Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_Gm831|Gm12962_chr4_131632431_F Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_Gm831|Gm12962_chr4_131632431_R Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_Gm14647|Gm25851_chrX_58295591_F Primer3: not found at this Tm N.d. 0.38
mm4_intergenic_Gm14647|Gm25851_chrX_58295591_R Primer3: not found at this Tm N.d. 0.38
mm3_exon_Ttll9_chr2_153006976_F TCGTCGGCAGCGTCCTTCAAGCGCAGAGGAAAGC 59.8 0.36
mm3_exon_Ttll9_chr2_153006976_R GTCTCGTGGGCTCGGCCTGCCAGGGCCTCATTT 59.6 0.36
mm4_intron_Sept4_chr11_87585050_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_Sept4_chr11_87585050_R Primer3: not found at this Tm N.d. 0.36
mm4_intron_Gm14616_chr2_174858740_F Primer3: not found at this Tm N.d. 0.35
mm4_intron_Gm14616_chr2_174858740_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Msln|Narfl_chr17_25765968_F Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_Msln|Narfl_chr17_25765968_R Primer3: not found at this Tm N.d. 0.34
mm4_intron_Nos1_chr5_117901780_F TCGTCGGCAGCGTCGCATGCAAGGGGTTGTAAGC 60.1 0.34
mm4_intron_Nos1_chr5_117901780_R GTCTCGTGGGCTCGGTTTTGTGGCCACCTAGGAGA 58.5 0.34
mm4_intron_Epb4.1l4b_chr4_57029969_F Primer3: not found at this Tm N.d. 0.32
mm4_intron_Epb4.1l4b_chr4_57029969_R Primer3: not found at this Tm N.d. 0.32
mm4_intron_Atp8b5_chr4_43271402_F TCGTCGGCAGCGTCAAGCAGCCCTGGTATTGTCC 60.0 0.31
mm4_intron_Atp8b5_chr4_43271402_R GTCTCGTGGGCTCGGGCAGATCCCAGGTTATACGGT 59.3 0.31
mm4_exon_Zfp292_chr4_34811035_F TCGTCGGCAGCGTCTGGCCTTTCACATGAGCAAT 58.0 0.30
mm4_exon_Zfp292_chr4_34811035_R GTCTCGTGGGCTCGGCGACAAGGACAAGTCTTATGAGC 59.3 0.30
mm4_intergenic_Ccdc13|Gm17163_chr9_121820102_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Ccdc13|Gm17163_chr9_121820102_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm23752|Gm24815_chr12_6005925_F TCGTCGGCAGCGTCCCAGGAAAATCGTGTGCTGC 60.1 0.29
mm4_intergenic_Gm23752|Gm24815_chr12_6005925_R GTCTCGTGGGCTCGGGGCACAGATGATCCCCACAA 60.0 0.29
mm4_intron_E130309F12Rik_chr4_49068024_F TCGTCGGCAGCGTCGGGGACAAGCTTTGTGGCTA 60.2 0.26
mm4_intron_E130309F12Rik_chr4_49068024_R GTCTCGTGGGCTCGGTCGTGTGTGCATGTGACTCT 59.6 0.26
mm4_intergenic_Ap3b1|Tbca_chr13_94654331_F TCGTCGGCAGCGTCGCCACTGAGCATCTGTCAGT 60.0 0.26
mm4_intergenic_Ap3b1|Tbca_chr13_94654331_R GTCTCGTGGGCTCGGCTCTCAGTGGGATAGCGCTG 59.9 0.26
mm3_intergenic_Cnpy1|Rbm33_chr5_28288660_F TCGTCGGCAGCGTCTCCCTGTCCTTTAAAATGCCA 57.4 0.24
mm3_intergenic_Cnpy1|Rbm33_chr5_28288660_R GTCTCGTGGGCTCGGTTGTCAGCTGAGCAGGAGTG 59.9 0.24
mm4_intergenic_Gm15323|Ankrd55_chr13_112271515_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm15323|Ankrd55_chr13_112271515_R Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_Pcdh20|Gm25886_chr14_89296491_F Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_Pcdh20|Gm25886_chr14_89296491_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Arid5b|1700040L02Rik_chr10_68372482_F TCGTCGGCAGCGTCGACCACTTGTGAGGAACCCC 60.2 0.23
mm4_intergenic_Arid5b|1700040L02Rik_chr10_68372482_R GTCTCGTGGGCTCGGCATACCGAGAAGTGTGCTGGT 60.0 0.23
mm3_intron_Tspan11_chr6_127911954_F TCGTCGGCAGCGTCCCCCGGCTAAAACTGAAGGT 59.9 0.23
mm3_intron_Tspan11_chr6_127911954_R GTCTCGTGGGCTCGGGGAACACAAAGCAAGGAGGC 59.6 0.23
mm3_intergenic_Gm26651|Diras2_chr13_52037477_F TCGTCGGCAGCGTCTCTGCTGTGACAAGGACTCT 58.2 0.23
mm3_intergenic_Gm26651|Diras2_chr13_52037477_R GTCTCGTGGGCTCGGGCGTGTGCAGAGGATACACT 60.1 0.23
mm4_intergenic_Gm25684|Gm9966_chr7_95946774_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm25684|Gm9966_chr7_95946774_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm14940|Gm17521_chrX_122776409_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm14940|Gm17521_chrX_122776409_R Primer3: not found at this Tm N.d. 0.22
mm3_intron_Obfc1_chr19_47532822_F TCGTCGGCAGCGTCAGATCCATCTCAAGAAACAAGATCA 58.0 0.22
mm3_intron_Obfc1_chr19_47532822_R GTCTCGTGGGCTCGGGCTTTGCAGGATACCTTCCCT 60.0 0.22
mm3_intergenic_1700092K14Rik|Gm11690_chr11_114218182_F TCGTCGGCAGCGTCTCCACATCCCCGATCTAGCA 60.1 0.21
mm3_intergenic_1700092K14Rik|Gm11690_chr11_114218182_R GTCTCGTGGGCTCGGCTCCTCTGATGAAAGCCCGG 60.1 0.21
mm4_intron_A730017C20Rik_chr18_59074874_F TCGTCGGCAGCGTCGCTGTGGAAGGAACCCTGAA 59.8 0.21
mm4_intron_A730017C20Rik_chr18_59074874_R GTCTCGTGGGCTCGGTCTCATCTCTGTGTCAGGGGT 59.9 0.21
mm2_intergenic_Gm5633|Gm22908_chr12_21835456_F Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm5633|Gm22908_chr12_21835456_R Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm3993|Gm10479_chr12_20368451_F Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm3993|Gm10479_chr12_20368451_R Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm10480|5730507C01Rik_chr12_18454945_F Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm10480|5730507C01Rik_chr12_18454945_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Tjp1_chr7_65333475_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Tjp1_chr7_65333475_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm26396|Gm13795_chr2_95064329_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm26396|Gm13795_chr2_95064329_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Mir1199|Palm3_chr8_84012898_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Mir1199|Palm3_chr8_84012898_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm5432|Lpin1_chr12_16362874_F TCGTCGGCAGCGTCGCCCAGAAATCGCCCATCAT 60.8 0.20
mm4_intergenic_Gm5432|Lpin1_chr12_16362874_R GTCTCGTGGGCTCGGGTGGAGAAGTTGAGACCCGG 60.0 0.20
mm4_intron_Abcd2_chr15_91158886_F Primer3: not found at this Tm N.d. 0.20
mm4_intron_Abcd2_chr15_91158886_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Cntln_chr4_85066653_F TCGTCGGCAGCGTCGAACGCGATGCAGATTGACC 59.9 0.20
mm4_intron_Cntln_chr4_85066653_R GTCTCGTGGGCTCGGTGCTACTTCGCTGCACTTCA 59.9 0.20
mm4_exon_Gm16938_chr7_98185042_F Primer3: not found at this Tm N.d. 0.19
mm4_exon_Gm16938_chr7_98185042_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Bcas3_chr11_85488593_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Bcas3_chr11_85488593_R Primer3: not found at this Tm N.d. 0.19
mm4_exon_Atp5b_chr10_128085621_F Primer3: not found at this Tm N.d. 0.19
mm4_exon_Atp5b_chr10_128085621_R Primer3: not found at this Tm N.d. 0.19
mm3_intergenic_Ankfn1|Pctp_chr11_89786729_F Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_Ankfn1|Pctp_chr11_89786729_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm13662/Gm13663/Gm17250|Gm13662_chr2_71755461_F TCGTCGGCAGCGTCCAACGGTTCCATCCTAGTGCT 60.0 0.18
mm4_intergenic_Gm13662/Gm13663/Gm17250|Gm13662_chr2_71755461_R GTCTCGTGGGCTCGGCAGGTTTCCTGCCAAGCATG 59.7 0.18
mm4_intergenic_Pitx1|H2afy_chr13_55965871_F TCGTCGGCAGCGTCTCCCCACTCTGTGATCACCT 59.8 0.18
mm4_intergenic_Pitx1|H2afy_chr13_55965871_R GTCTCGTGGGCTCGGCTCCCACAGAGCCAGCAG 59.7 0.18
mm4_intergenic_Gm11493|Akap1_chr11_88774231_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm11493|Akap1_chr11_88774231_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Auts2_chr5_132370419_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Auts2_chr5_132370419_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Lingo1|Odf3l1_chr9_56782482_F TCGTCGGCAGCGTCCTGAAGGGCCTGCTCTACAC 60.1 0.18
mm4_intergenic_Lingo1|Odf3l1_chr9_56782482_R GTCTCGTGGGCTCGGCACTGAGTGAGGGTGAGCTC 59.7 0.18
mm4_exon_Hipk2_chr6_38818347_F Primer3: not found at this Tm N.d. 0.18
mm4_exon_Hipk2_chr6_38818347_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_D19Bwg1357e_chr19_27408949_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_D19Bwg1357e_chr19_27408949_R Primer3: not found at this Tm N.d. 0.17
mm3_intron_Tcf7l2_chr19_55846167_F Primer3: not found at this Tm N.d. 0.17
mm3_intron_Tcf7l2_chr19_55846167_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Cdk1|Ank3_chr10_69517804_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Cdk1|Ank3_chr10_69517804_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Plec_chr15_76190787_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Plec_chr15_76190787_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Etl4|Gm13335_chr2_20408628_F TCGTCGGCAGCGTCGAGGCATCCTGTTTCCTCCC 60.1 0.17
mm4_intergenic_Etl4|Gm13335_chr2_20408628_R GTCTCGTGGGCTCGGAAAAGACAGCCCACCCTCTG 59.8 0.17
mm3_intron_Ssc5d_chr7_4941130_F Primer3: not found at this Tm N.d. 0.16
mm3_intron_Ssc5d_chr7_4941130_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_A830092H15Rik|Gm25773_chr3_26421027_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_A830092H15Rik|Gm25773_chr3_26421027_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Pla2r1_chr2_60498880_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Pla2r1_chr2_60498880_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm25460|Gm5263_chr1_145555486_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm25460|Gm5263_chr1_145555486_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Smad7_chr18_75386685_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Smad7_chr18_75386685_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Pgrmc2|Gm25487_chr3_41375132_F TCGTCGGCAGCGTCTGGGATGGATGGAATCAAGCA 59.4 0.14
mm4_intergenic_Pgrmc2|Gm25487_chr3_41375132_R GTCTCGTGGGCTCGGTGGCTGAGTTCTCCAGCAAG 59.9 0.14
mm4_intergenic_Abca12|Atic_chr1_71473034_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Abca12|Atic_chr1_71473034_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm24792|Epha5_chr5_83771875_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm24792|Epha5_chr5_83771875_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Chrm3|Gm26861_chr13_9880557_F TCGTCGGCAGCGTCTCCCAAGGCTTCTTGTCTGTC 59.9 0.13
mm4_intergenic_Chrm3|Gm26861_chr13_9880557_R GTCTCGTGGGCTCGGCCAGGTGACAAATGCCTGTC 59.1 0.13
mm4_intergenic_Gm23104|Hsp25-ps1/Gm9817_chr13_45072887_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm23104|Hsp25-ps1/Gm9817_chr13_45072887_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Tmem59l|Crlf1_chr8_70489843_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Tmem59l|Crlf1_chr8_70489843_R Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Gm14920|Gm14918_chrX_117238941_F Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Gm14920|Gm14918_chrX_117238941_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Bai2_chr4_130013732_F TCGTCGGCAGCGTCTGCTGAACTCTGCTGTTGGG 60.5 0.12
mm4_exon_Bai2_chr4_130013732_R GTCTCGTGGGCTCGGGGCGACACCTCCGTATCATT 59.8 0.12
mm4_exon_Jakmip1_chr5_37085451_F Primer3: not found at this Tm N.d. 0.12
mm4_exon_Jakmip1_chr5_37085451_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Kdm2b_chr5_122881263_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Kdm2b_chr5_122881263_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Adc|Ak2_chr4_128967344_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Adc|Ak2_chr4_128967344_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_4930401O12Rik|Gm11376_chr13_31284016_F TCGTCGGCAGCGTCCAGCAGGTCATGAGGGGAAG 60.1 0.11
mm4_intergenic_4930401O12Rik|Gm11376_chr13_31284016_R GTCTCGTGGGCTCGGAGGGCCATTAACAGTGCCTG 60.3 0.11
mm4_intergenic_Zfp280d/BC065403|RP24-282D16.2_chr9_72368414_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Zfp280d/BC065403|RP24-282D16.2_chr9_72368414_R Primer3: not found at this Tm N.d. 0.11
mm2_intergenic_Gm16102|Gulp1_chr1_44650724_F Primer3: not found at this Tm N.d. 0.10
mm2_intergenic_Gm16102|Gulp1_chr1_44650724_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_M5C1000I18Rik/C2cd4b|C2cd4a_chr9_67815651_F TCGTCGGCAGCGTCACTTGTCTGTGCCAGAAACA 57.5 0.10
mm4_intergenic_M5C1000I18Rik/C2cd4b|C2cd4a_chr9_67815651_R GTCTCGTGGGCTCGGAGAAAATAGGTTGCATTGATTTGC 57.0 0.10
mm4_intergenic_Gm25937|Cxcl13_chr5_95901734_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm25937|Cxcl13_chr5_95901734_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm20828|Gm20812_chrY_11412102_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm20828|Gm20812_chrY_11412102_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm20822|Gm21809_chrY_16097452_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm20822|Gm21809_chrY_16097452_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Rheb|Prkag2_chr5_24846127_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Rheb|Prkag2_chr5_24846127_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_4930596D02Rik|Gm7853_chr14_35845921_F TCGTCGGCAGCGTCCGGAAAGGGCAGAGGCTATG 60.5 0.09
mm4_intergenic_4930596D02Rik|Gm7853_chr14_35845921_R GTCTCGTGGGCTCGGACATTAGGGAAAACAGGGCCC 60.2 0.09
mm4_intergenic_Gm16685|Gm24614_chr3_8411271_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm16685|Gm24614_chr3_8411271_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sulf2_chr2_166110475_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sulf2_chr2_166110475_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sergef_chr7_46637407_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sergef_chr7_46637407_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Pld5_chr1_176166233_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Pld5_chr1_176166233_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ccnd2_chr6_127145313_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ccnd2_chr6_127145313_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Wdr11|Gm23847_chr7_129822522_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Wdr11|Gm23847_chr7_129822522_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Prickle1|Adamts20_chr15_93730344_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Prickle1|Adamts20_chr15_93730344_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Palm2/Gm20459|Gm20459/Palm2_chr4_57621989_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Palm2/Gm20459|Gm20459/Palm2_chr4_57621989_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Il17rd|Arhgef3_chr14_27132025_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Il17rd|Arhgef3_chr14_27132025_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm6984|Htr2a_chr14_74602643_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm6984|Htr2a_chr14_74602643_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cidea|Tubb6_chr18_67368586_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cidea|Tubb6_chr18_67368586_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Chst3|Gm23488_chr10_60221178_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Chst3|Gm23488_chr10_60221178_R Primer3: not found at this Tm N.d. 0.09
mm2_intron_Gmpr_chr13_45531582_F Primer3: not found at this Tm N.d. 0.09
mm2_intron_Gmpr_chr13_45531582_R Primer3: not found at this Tm N.d. 0.09
mm2_intergenic_Zfand5|Gda_chr19_21372430_F Primer3: not found at this Tm N.d. 0.09
mm2_intergenic_Zfand5|Gda_chr19_21372430_R Primer3: not found at this Tm N.d. 0.09
mm3_intron_Mei1_chr15_82120010_F Primer3: not found at this Tm N.d. 0.09
mm3_intron_Mei1_chr15_82120010_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Prex1_chr2_166616685_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Prex1_chr2_166616685_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Loxl4|Gm25216_chr19_42655072_F TCGTCGGCAGCGTCCCAGATTTGCCCACCAGTCA 60.2 0.08
mm4_intergenic_Loxl4|Gm25216_chr19_42655072_R GTCTCGTGGGCTCGGGGGGAGGGAAGAGAGGAAGA 59.6 0.08
mm3_intron_Cyfip2_chr11_46242064_F Primer3: not found at this Tm N.d. 0.08
mm3_intron_Cyfip2_chr11_46242064_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Parp16|Cilp_chr9_65258292_F TCGTCGGCAGCGTCATCCCTTCAGAGTCGAGCCT 60.0 0.08
mm4_intergenic_Parp16|Cilp_chr9_65258292_R GTCTCGTGGGCTCGGGGAAAGGCTGCTCTGAAGGT 59.9 0.08
mm4_intron_Npas2_chr1_39342009_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Npas2_chr1_39342009_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Abcc5_chr16_20392660_F TCGTCGGCAGCGTCCAGCTGCCTCGACTTCTCTT 59.7 0.08
mm4_exon_Abcc5_chr16_20392660_R GTCTCGTGGGCTCGGGGACTCCTCCCACTCCAGTA 59.6 0.08
mm4_intergenic_5430435G22Rik|Slc26a9_chr1_131739128_F TCGTCGGCAGCGTCACAGACATGCACAGCCTTCT 59.6 0.08
mm4_intergenic_5430435G22Rik|Slc26a9_chr1_131739128_R GTCTCGTGGGCTCGGGTCCTGTCTCCACATCTGGC 60.1 0.08
mm4_intron_B3galt1_chr2_68013352_F TCGTCGGCAGCGTCGTGATCTCGTTGTTGGTCTCT 57.6 0.07
mm4_intron_B3galt1_chr2_68013352_R GTCTCGTGGGCTCGGGCTTCTTTGACAGAGTAGCCT 57.6 0.07
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88906542_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88906542_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_AC112986.1|Tsn_chr1_117578699_F TCGTCGGCAGCGTCACAACCTCTTCATGCACAAGC 59.3 0.07
mm4_intergenic_AC112986.1|Tsn_chr1_117578699_R GTCTCGTGGGCTCGGGAGCAGAGACCAGCACTGAG 60.1 0.07
mm4_intergenic_Gm12130|Gm23520_chr11_38566841_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12130|Gm23520_chr11_38566841_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Cda_chr4_138351230_F TCGTCGGCAGCGTCCTGCTATAACTGGCCCACCC 60.1 0.07
mm4_exon_Cda_chr4_138351230_R GTCTCGTGGGCTCGGAAACGCCTGCTACCCACTAG 59.7 0.07
mm4_intron_9530036O11Rik_chr5_28893799_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_9530036O11Rik_chr5_28893799_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12494339_F TCGTCGGCAGCGTCCTCAGCTTGACAGGCCTCTC 60.1 0.07
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12494339_R GTCTCGTGGGCTCGGTACGGGCCTAAGCAAGAAGC 60.1 0.07
mm4_intron_Slc10a7_chr8_78589961_F TCGTCGGCAGCGTCGCCTGTCATTTGAGCTGGGA 60.3 0.07
mm4_intron_Slc10a7_chr8_78589961_R GTCTCGTGGGCTCGGAGGGAGTCGATCAAGGGGAA 59.9 0.07
mm4_intergenic_Slc39a8|Bank1_chr3_135971612_F TCGTCGGCAGCGTCTTCCTGAGCTGCCTCCAAAG 59.9 0.06
mm4_intergenic_Slc39a8|Bank1_chr3_135971612_R GTCTCGTGGGCTCGGTGCCACTGACCAGGTTTTCT 59.4 0.06
mm4_intron_Dapp1_chr3_137961371_F TCGTCGGCAGCGTCATGCAATGTTCTCAGCCCCA 59.9 0.06
mm4_intron_Dapp1_chr3_137961371_R GTCTCGTGGGCTCGGTGGAAGCGAGACAGGTAAGC 59.7 0.06
mm4_intergenic_Gm21874|Gm20821_chrY_9712585_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm21874|Gm20821_chrY_9712585_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm21820|AC132591.1_chrY_5455548_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm21820|AC132591.1_chrY_5455548_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm20828|Gm20812_chrY_11350449_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm20828|Gm20812_chrY_11350449_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm20822|Gm21809_chrY_16035857_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm20822|Gm21809_chrY_16035857_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mrpl36|Lpcat1_chr13_73336010_F TCGTCGGCAGCGTCTAGCAGGCCAAGACAAAGGG 59.9 0.06
mm4_intergenic_Mrpl36|Lpcat1_chr13_73336010_R GTCTCGTGGGCTCGGCTGTGCTCTTCAGCCCTTCA 59.9 0.06
mm4_intron_Rims1_chr1_22385871_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Rims1_chr1_22385871_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Ephx2|Chrna2_chr14_66135949_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Ephx2|Chrna2_chr14_66135949_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Polr3k_chr2_181869112_F TCGTCGGCAGCGTCGGTCTACAGTAGAGCTTGTGC 58.1 0.06
mm4_exon_Polr3k_chr2_181869112_R GTCTCGTGGGCTCGGCACTCTCATCTGAAGTTGGTCCA 59.9 0.06
mm4_intergenic_Rpap2|Gm26692_chr5_107665816_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rpap2|Gm26692_chr5_107665816_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Synm_chr7_67757850_F TCGTCGGCAGCGTCGACCAACTTCCTGAGAGCCC 60.0 0.06
mm4_intron_Synm_chr7_67757850_R GTCTCGTGGGCTCGGGCTCAGTTTGGCCCTAGTGT 59.9 0.06
mm4_intron_Herc2_chr7_56188312_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Herc2_chr7_56188312_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Asap2|Gm21989/Asap2_chr12_21148775_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Asap2|Gm21989/Asap2_chr12_21148775_R Primer3: not found at this Tm N.d. 0.06
mm3_intron_Mertk_chr2_128770959_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Mertk_chr2_128770959_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26106|Gm7729_chr18_27340339_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26106|Gm7729_chr18_27340339_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26686|Gm23056_chr1_128860783_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26686|Gm23056_chr1_128860783_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Amer2|Spata13_chr14_60439894_F TCGTCGGCAGCGTCTCACTCAGTGGCAAAGGACC 59.8 0.05
mm4_intergenic_Amer2|Spata13_chr14_60439894_R GTCTCGTGGGCTCGGAGGTTGATGGACCCTGGTCT 60.1 0.05
mm3_intergenic_Gm9081|Avpr1a_chr10_122376681_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm9081|Avpr1a_chr10_122376681_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Prdm6_chr18_53509487_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Prdm6_chr18_53509487_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Pafah2_chr4_134417051_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Pafah2_chr4_134417051_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Lrig1_chr6_94610913_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Lrig1_chr6_94610913_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Ints6_chr14_62729395_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Ints6_chr14_62729395_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_D15Ertd621e_chr15_58440773_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_D15Ertd621e_chr15_58440773_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Ntsr2|Greb1_chr12_16670278_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Ntsr2|Greb1_chr12_16670278_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Jmjd1c|Nrbf2_chr10_67265345_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Jmjd1c|Nrbf2_chr10_67265345_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm5530|mmu-mir-6344_chr1_81788968_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm5530|mmu-mir-6344_chr1_81788968_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm5335|A730056A06Rik_chr7_73285709_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm5335|A730056A06Rik_chr7_73285709_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26181|Triml1_chr8_43038093_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm26181|Triml1_chr8_43038093_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm16036|Sdk1_chr5_141764042_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm16036|Sdk1_chr5_141764042_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm15401|Tcf7l1_chr6_72644375_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm15401|Tcf7l1_chr6_72644375_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm12661|Olfr1377_chr11_50983473_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm12661|Olfr1377_chr11_50983473_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Col6a3|Mlph_chr1_90844420_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Col6a3|Mlph_chr1_90844420_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_B930082K07Rik/Lrrc49|Larp6_chr9_60709379_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_B930082K07Rik/Lrrc49|Larp6_chr9_60709379_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Agpat4|Tdgf1-ps2_chr17_12160500_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Agpat4|Tdgf1-ps2_chr17_12160500_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_4933440J02Rik|Krr1_chr10_111872379_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_4933440J02Rik|Krr1_chr10_111872379_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Arhgap24_chr5_102573277_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Arhgap24_chr5_102573277_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Thsd4_chr9_60484280_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Thsd4_chr9_60484280_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm13935|Gm10794_chr2_110593703_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm13935|Gm10794_chr2_110593703_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ccdc171|Gm11415_chr4_83867899_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ccdc171|Gm11415_chr4_83867899_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ttc17_chr2_94375022_F TCGTCGGCAGCGTCTGGGAAGGAAACAGTCTGAGA 58.3 0.04
mm4_intron_Ttc17_chr2_94375022_R GTCTCGTGGGCTCGGACCAGCTATTACACACTGGGG 59.4 0.04
mm4_intron_Camta1_chr4_151270871_F TCGTCGGCAGCGTCAATTCTGCAGACGGGCTTGT 60.2 0.04
mm4_intron_Camta1_chr4_151270871_R GTCTCGTGGGCTCGGCATTATGCGCTGTTGAGGCC 59.9 0.04
mm3_intron_Rnd1_chr15_98669122_F Primer3: not found at this Tm N.d. 0.04
mm3_intron_Rnd1_chr15_98669122_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Zfp536_chr7_37602286_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Zfp536_chr7_37602286_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23694|Igkv7-33_chr6_70035735_F TCGTCGGCAGCGTCAAGCCACTCACATCCTCGTG 60.0 0.04
mm4_intergenic_Gm23694|Igkv7-33_chr6_70035735_R GTCTCGTGGGCTCGGTGGAGTGAACTCTTGGCCTG 59.6 0.04
mm4_intron_Prkca_chr11_108017302_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Prkca_chr11_108017302_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm5161|Ephb1_chr9_101527177_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm5161|Ephb1_chr9_101527177_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Lin7b|Snrnp70_chr7_45376197_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Lin7b|Snrnp70_chr7_45376197_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm6990|A630007B06Rik_chr19_56769262_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm6990|A630007B06Rik_chr19_56769262_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Cachd1_chr4_100897753_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Cachd1_chr4_100897753_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Tgm5_chr2_121048304_F TCGTCGGCAGCGTCGGGATGTCCTCTTGAGCAGG 59.8 0.03
mm4_intron_Tgm5_chr2_121048304_R GTCTCGTGGGCTCGGGACTTTCCCGGGCATCATGA 60.1 0.03
mm4_exon_Phlda3_chr1_135768891_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Phlda3_chr1_135768891_R Primer3: not found at this Tm N.d. 0.03
mm2_intron_Col12a1_chr9_79641261_F Primer3: not found at this Tm N.d. 0.03
mm2_intron_Col12a1_chr9_79641261_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm7719|Gm24377_chr12_59551411_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm7719|Gm24377_chr12_59551411_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25761|Magi2_chr5_20118646_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25761|Magi2_chr5_20118646_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_1700080O16Rik|Gpc4_chrX_52017857_F TCGTCGGCAGCGTCATTCTTGGCAGTCTGGCTCC 60.0 0.03
mm4_intergenic_1700080O16Rik|Gpc4_chrX_52017857_R GTCTCGTGGGCTCGGGGCCCATGGAGGTTTCTTGA 59.9 0.03
mm4_intergenic_1700017N19Rik|Gm26278_chr10_100829313_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_1700017N19Rik|Gm26278_chr10_100829313_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Olfr251|Olfr147_chr9_38399664_F TCGTCGGCAGCGTCAGACGATGATATTACCCATGTGGA 59.1 0.02
mm4_intergenic_Olfr251|Olfr147_chr9_38399664_R GTCTCGTGGGCTCGGGGTCTCGATATAGATTTGTGGAAGT 58.2 0.02
mm4_intergenic_Id3|E2f2_chr4_136162297_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Id3|E2f2_chr4_136162297_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm10295|Mctp2_chr7_71457299_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm10295|Mctp2_chr7_71457299_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11922|Map3k7_chr4_31731707_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11922|Map3k7_chr4_31731707_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rnf114_chr2_167512664_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rnf114_chr2_167512664_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45958616_F TCGTCGGCAGCGTCCAGGAGGAGAGGGACCAAGA 59.9 0.02
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45958616_R GTCTCGTGGGCTCGGAGCCAGTGGGTTCCTTTGAA 59.4 0.02
mm4_intergenic_Osmr|Lifr_chr15_7041175_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Osmr|Lifr_chr15_7041175_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sh3gl2|Gm12413_chr4_85225497_F TCGTCGGCAGCGTCGTGTTGAGCATTGGCAGCTC 60.1 0.02
mm4_intergenic_Sh3gl2|Gm12413_chr4_85225497_R GTCTCGTGGGCTCGGGACAAAAGGGTGAGGAGGGG 59.9 0.02
mm4_intron_Xylt1_chr7_117476793_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Xylt1_chr7_117476793_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Sardh_chr2_27242646_F TCGTCGGCAGCGTCTTTACACCCCACCTTTCCCC 59.5 0.02
mm4_intron_Sardh_chr2_27242646_R GTCTCGTGGGCTCGGACAAGAGGCTCATGTCGGTG 60.0 0.02
mm4_intergenic_Gm17212|Gm17213_chr3_69393254_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm17212|Gm17213_chr3_69393254_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Erc2_chr14_28335082_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Erc2_chr14_28335082_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gns_chr10_121387903_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Gns_chr10_121387903_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Dennd1a_chr2_37984245_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Dennd1a_chr2_37984245_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tas2r140|2700089E24Rik_chr6_133066446_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tas2r140|2700089E24Rik_chr6_133066446_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Runx1|AC124326.1_chr16_93055636_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Runx1|AC124326.1_chr16_93055636_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Smad2|Skor2_chr18_76718548_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Smad2|Skor2_chr18_76718548_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cyp2f2_chr7_27132058_F TCGTCGGCAGCGTCACCATTCTTGGAGGACACCC 59.3 0.01
mm4_intron_Cyp2f2_chr7_27132058_R GTCTCGTGGGCTCGGCCCTTGGTCCTGAGCTTTGT 59.8 0.01
mm2_intergenic_Zc3h12a|1700041M05Rik_chr4_125225750_F Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Zc3h12a|1700041M05Rik_chr4_125225750_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Slc12a8_chr16_33522337_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Slc12a8_chr16_33522337_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Mlph_chr1_90940873_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Mlph_chr1_90940873_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Kctd1_chr18_15086619_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Kctd1_chr18_15086619_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ipmk_chr10_71369069_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ipmk_chr10_71369069_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm2373|5330416C01Rik_chr13_97576027_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm2373|5330416C01Rik_chr13_97576027_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm20646|Mrps9_chr1_42777228_F TCGTCGGCAGCGTCCCCAGCTTCTACAGAACTCGG 60.1 0.01
mm4_intergenic_Gm20646|Mrps9_chr1_42777228_R GTCTCGTGGGCTCGGATGGCCGAAGAAGAAAGGGT 59.3 0.01
mm4_intergenic_Gm25722|Gm10051_chr5_132826276_F TCGTCGGCAGCGTCACACCCAGATTTGGTAGGGC 59.6 0.01
mm4_intergenic_Gm25722|Gm10051_chr5_132826276_R GTCTCGTGGGCTCGGAGCCAGCTCTTTCCCAACTC 59.9 0.01
mm4_intergenic_Stmn4|Gm23899_chr14_66455265_F TCGTCGGCAGCGTCAGCATAGATACCCCTCTGGCT 59.8 0.01
mm4_intergenic_Stmn4|Gm23899_chr14_66455265_R GTCTCGTGGGCTCGGGAACTTTGGAGGCCTGGACA 59.8 0.01
mm4_intron_Grm7_chr6_111252489_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Grm7_chr6_111252489_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26291|Gm17377_chr17_55165252_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26291|Gm17377_chr17_55165252_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cdh13_chr8_118505925_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cdh13_chr8_118505925_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Galnt18_chr7_111744506_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Galnt18_chr7_111744506_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gfra2|Gm9192_chr14_71694369_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gfra2|Gm9192_chr14_71694369_R Primer3: not found at this Tm N.d. 0.01
mm2_intron_Iqgap2_chr13_95733699_F Primer3: not found at this Tm N.d. 0.01
mm2_intron_Iqgap2_chr13_95733699_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ldlrap1_chr4_134759592_F TCGTCGGCAGCGTCGAGCCTGGGTTCACAGAGAG 59.7 0.01
mm4_intron_Ldlrap1_chr4_134759592_R GTCTCGTGGGCTCGGAAGGAGGAATTGGGGCATGG 60.0 0.01
mm3_intron_B4galnt4_chr7_141063761_F TCGTCGGCAGCGTCCGGAAGTGTTCAGCAGTCCT 59.9 0.01
mm3_intron_B4galnt4_chr7_141063761_R GTCTCGTGGGCTCGGCTTCTCCCCATCTGCAGACG 60.1 0.01
mm4_intergenic_Aida|Mia3_chr1_183325514_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Aida|Mia3_chr1_183325514_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_9230115E21Rik|Fdx1_chr9_51914678_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_9230115E21Rik|Fdx1_chr9_51914678_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Rfx1_chr8_84091361_F TCGTCGGCAGCGTCAGCACCCCACTACCCCTTC 60.9 0.00
mm4_exon_Rfx1_chr8_84091361_R GTCTCGTGGGCTCGGTGATGTCTGACAGCCCTGTG 59.6 0.00
mm4_intergenic_Tmem132e|Gm24612_chr11_82453635_F TCGTCGGCAGCGTCTGGCTGCCAGCTCTTCTAAC 60.0 0.00
mm4_intergenic_Tmem132e|Gm24612_chr11_82453635_R GTCTCGTGGGCTCGGGCCATTGCTGCAGAAATCCC 60.1 0.00
mm4_intergenic_Gm9885|Mrpl46_chr7_78759222_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9885|Mrpl46_chr7_78759222_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ctnna3|Gm22829_chr10_65291155_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ctnna3|Gm22829_chr10_65291155_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Stox2|4930448N21Rik/Gm10083_chr8_47406021_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Stox2|4930448N21Rik/Gm10083_chr8_47406021_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25183|Spats2_chr15_99147124_F TCGTCGGCAGCGTCACCTTCTCATTACCTTTCTCCACC 60.0 0.00
mm4_intergenic_Gm25183|Spats2_chr15_99147124_R GTCTCGTGGGCTCGGGGAACCTTGAAGTGTTTCACCG 59.9 0.00
mm4_intergenic_F3|Abcd3_chr3_121756433_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_F3|Abcd3_chr3_121756433_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tsku|Gucy2d_chr7_98404813_F TCGTCGGCAGCGTCAGACTCCCAGCCCTGTATGT 59.9 0.00
mm4_intergenic_Tsku|Gucy2d_chr7_98404813_R GTCTCGTGGGCTCGGAGGGATTGTGTGTTCTCTGGA 58.6 0.00
mm4_intron_Fhad1_chr4_141920661_F TCGTCGGCAGCGTCCCGAGGGCTTCTAGCATCG 60.0 0.00
mm4_intron_Fhad1_chr4_141920661_R GTCTCGTGGGCTCGGTGGGGCTGCAGAAGAAACTC 60.2 0.00
mm4_intergenic_Imp4|Ptpn18_chr1_34447579_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Imp4|Ptpn18_chr1_34447579_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24932|Syt9_chr7_107353016_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24932|Syt9_chr7_107353016_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam172a_chr13_77804238_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam172a_chr13_77804238_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pcdh15_chr10_73724816_F TCGTCGGCAGCGTCAGAGTCTTGCTTGTGAGGTGT 59.2 0.00
mm4_intron_Pcdh15_chr10_73724816_R GTCTCGTGGGCTCGGCCAGACGTTGTCCTCTCACC 60.0 0.00
mm4_intergenic_Wnt9b|Wnt3_chr11_103753156_F TCGTCGGCAGCGTCGGGGCTCTTCGGTTTGTCTT 60.2 0.00
mm4_intergenic_Wnt9b|Wnt3_chr11_103753156_R GTCTCGTGGGCTCGGTCTTCTGGGTGAGCTCTTGC 59.6 0.00
mm4_intron_Vipr1_chr9_121666701_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Vipr1_chr9_121666701_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9949|Htr4_chr18_62322615_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9949|Htr4_chr18_62322615_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Camsap2_chr1_136343821_F TCGTCGGCAGCGTCAAGCACACCAGACTCCAGTG 59.8 0.00
mm4_intron_Camsap2_chr1_136343821_R GTCTCGTGGGCTCGGCTTCTGAGCAGAAACGCCCT 60.3 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm4_intergenic_Myh9|Gm22107_chr15_77824847 CCCAAGACCTTGTCTCGCTTTCTGTATCATTACAGAAAACATAGGTCCCAGCTGACTTCCACTCAGAAAAAACTGGTGCT
GGTGAAATGTAACG
mm3_exon_Ttll9_chr2_153006976 CTTCAAGCGCAGAGGAAAGCCCCTAGCTGACCCCCAGTCAGAAGAAATGAGGCCCTGGCAGG
mm4_intron_Nos1_chr5_117901780 GCATGCAAGGGGTTGTAAGCATGGTTCCCAGCTGACCCCAACCAAGAGATCTCCTAGGTGGCCACAAAA
mm4_intron_Atp8b5_chr4_43271402 AAGCAGCCCTGGTATTGTCCCATCCTGCACTGTACTGTACTTCAGTTTGTGCTTCCAGGACTTCTGTTCAAGCACCAAAC
TGACCCCCTCCTAGAAA
CACCGTATAACCTGGGATCTGC
mm4_exon_Zfp292_chr4_34811035 TGGCCTTTCACATGAGCAATTAAATTTTTGAAGTACTTAAAGCCCTTTTTACAAAAGGTCACAGGACAATTAAACTCATT
AACAGGTACTGGTTTCTGGACAGGGATCACCTCGGGCTCATAAGACTTGTCCTTGTCG
mm4_intergenic_Gm23752|Gm24815_chr12_6005925 CCAGGAAAATCGTGTGCTGCATGTGATCCAGGATGACTCTCAAAGGTCAATTTCAATAGATGGCAATCAGCTGGCGATCC
TGGATGGGAATCAGCTGGG
GAGCATTGTGGGGATCATCTGTGCC
mm4_intron_E130309F12Rik_chr4_49068024 GGGGACAAGCTTTGTGGCTAGTCAGTGTTTACTGGCAGGGAGTCAGCTTGGCATCCATGCAGGTAGTCCTTCAGCTATGG
CAAATTTATGTTTTTCATCCATCACTAACAACCTGGAACAGAGTCACATGCACACACGA
mm4_intergenic_Ap3b1|Tbca_chr13_94654331 GCCACTGAGCATCTGTCAGTATGGAAATCAGCACTCCTAGCTGACCCCGATTGAGAACTCAGTGACAGCCCCACAATGCA
AGGCGTCCCCTCTATCACCCGGAGGAGCAGGTGCCACCCAGCGCTATCCCACTGAGAG
mm3_intergenic_Cnpy1|Rbm33_chr5_28288660 TCCCTGTCCTTTAAAATGCCAAACAAATCATGAGACATGCCTTCCTAACCCCAGCTGACCCCCAGCAAGAAGTCTCCTTA
AGACAAGCTGATTCCCTGATATCAGATTAGAATTAACCCACTCCTGCTCAGCTGACAA
mm4_intergenic_Arid5b|1700040L02Rik_chr10_68372482 GACCACTTGTGAGGAACCCCCAGTTACTAGTATTTAAATGAAAATCTGCAGATTTGTGATTTCTGGGGAGGGGTCAGCAT
GG
ACCAGCACACTTCTCGGTATG
mm3_intron_Tspan11_chr6_127911954 CCCCGGCTAAAACTGAAGGTTTCCAAGAGAACAGAGTGTGTACATTTCCCCAGCTGTCCTCCATCCAGGAACTACTGCAA
AGCCTCCTTGCTTTGTGTTCC
mm3_intergenic_Gm26651|Diras2_chr13_52037477 TCTGCTGTGACAAGGACTCTATTGTTTATCACTGAACCTTGACCGATACAGTGAGTGACACACTCGATTTCTTAACACGA
AACTATCGTTGCATTTCTGGACGGAGTTCAGCTTGGTCAGAGTGTATCCTCTGCACACGC
mm3_intron_Obfc1_chr19_47532822 AGATCCATCTCAAGAAACAAGATCAAAAACCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNN
NNNNNNNNNNNNNNNNNNNNNNTCAGGGAAGGTATCCTGCAAAGC
mm3_intergenic_1700092K14Rik|Gm11690_chr11_114218182 TCCACATCCCCGATCTAGCAAGAAACTCCTTCAGGGTCAACCAAACGGCCACCTGGGTTCTCTAGGAAGCCCTAATGGCA
AATGGCTTTCAGAATGGGGCTCAGCTGGGGCGACTCAAGGCCGGGCTTTCATCAGAGGAG
mm4_intron_A730017C20Rik_chr18_59074874 GCTGTGGAAGGAACCCTGAACACCTAGCGTTATCCTTCCTGTATGGAAGTCAGCTGGGGATCTCTTGAAACTTAACCAAA
TTTTTACCCCTGACACAGAGATGAGA
mm4_intergenic_Gm5432|Lpin1_chr12_16362874 GCCCAGAAATCGCCCATCATCCCAGCTTAGCCCCATACAGACAGTCATGTCTCATCTCCGGGTCTCAACTTCTCCAC
mm4_intron_Cntln_chr4_85066653 GAACGCGATGCAGATTGACCCCCAGCTGACGCCAATGCAGAGAAGCTAGAACTCAAGCTTGCTGGTTTGACTATGAAGTG
CAGCGAAGTAGCA
mm4_intergenic_Gm13662/Gm13663/Gm17250|Gm13662_chr2_71755461 CAACGGTTCCATCCTAGTGCTATTTAGAGTGTTATAAAGGCCTAGTGCACACCCAAGTGACCCCCATCTAGAAGTTAAAG
TCTTTACATCAGTGGAATGTGGCTTAGGGATTCTTGGTCCATGCTTGGCAGGAAACCTG
mm4_intergenic_Pitx1|H2afy_chr13_55965871 TCCCCACTCTGTGATCACCTTCAAGACAAACAGAGCAGTTCCTCAGTGCCCCAGCTGATCCCCACCGAGCAAGCTGCTGG
CTCTGTGGGAG
mm4_intergenic_Lingo1|Odf3l1_chr9_56782482 CTGAAGGGCCTGCTCTACACTTTCTAGATGGAAGATGGCTTTCACTCATTAGGCCCCATCTGGCCCCTTGGAACCCATTC
CTAGCTGACCCTCAGCCACAAG
CCAGGTCCCAGGGCCACCGAGCTCACCCTCACTCAGTG
mm4_intergenic_Etl4|Gm13335_chr2_20408628 GAGGCATCCTGTTTCCTCCCAGACTCCTGTCTTCTATCATCCACCCCCACCCCAGTGTCACTCAGGGAAGAAGATGGCTG
CTTGTACTGGCTGGGGGACAGCTGGGCAGAGGGTGGGCTGTCTTTT
mm4_intergenic_Pgrmc2|Gm25487_chr3_41375132 TGGGATGGATGGAATCAAGCAGAAGGATCAGAGGTAGACATCCAACATCTGGATGTCGGTCAGCTTGGCGAGCAGCCAGA
GCCACAGGCAGGTGGGCTGGCCCTCTGTGGCAGCTTGCTGGAGAACTCAGCCA
mm4_intergenic_Chrm3|Gm26861_chr13_9880557 TCCCAAGGCTTCTTGTCTGTCGTCTCTCATCCTTTCTTGCTAAAGGGTGACAGATTCCTATGACTGGCTTTCTTTTGTGT
CCAAGCTGACACTCTTCCAGCAAGGTTTGAGACAGGCATTTGTCACCTGG
mm4_exon_Bai2_chr4_130013732 TGCTGAACTCTGCTGTTGGGCCTGGAGTGGCAGGGAAAGCCAGGATGTGCTGCTGCCTGGACTCCGGCCCAGCTCTCTGG
GGATGGGGGTCAGTTGGG
TTGGAGAATGATACGGAGGTGTCGCC
mm4_intergenic_4930401O12Rik|Gm11376_chr13_31284016 CAGCAGGTCATGAGGGGAAGAAAACGAACAGGAAGACACTAGACTCAAGAGAAACTATAGGCACTGCATACAGCCTTCTG
GAAAGGGGTGAGCTTGG
ACAGGAGCCGATGCAGGCACTGTTAATGGCCCT
mm4_intergenic_M5C1000I18Rik/C2cd4b|C2cd4a_chr9_67815651 ACTTGTCTGTGCCAGAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNAACGGTTTCTGGGTGGAGTTCAGCATGGATGCAAATCAATGCAACCTATTTTCT
mm4_intergenic_4930596D02Rik|Gm7853_chr14_35845921 CGGAAAGGGCAGAGGCTATGATTTCCAAGCTGACCTGGCTCCAGAAACATGTCCTGCCCCACAGGGCCCTGTTTTCCCTA
ATGT
mm4_intergenic_Loxl4|Gm25216_chr19_42655072 CCAGATTTGCCCACCAGTCAGTGAACTAAATCTCACGCTTTCAGGAGGTGGATCAGCTGGGAGTCCCATGAACAGGTCAG
TTCTTTTGGGATCTGTCCATCAGTGTGGTATTCCATCCTCTTCCTCTCTTCCCTCCCC
mm4_intergenic_Parp16|Cilp_chr9_65258292 ATCCCTTCAGAGTCGAGCCTGAGCTGAGCCCAGGCTGTGGTTCTATGGTTGGGGGCCAGCTTGGAACCTATCATCCATTC
ATCATTGCCCAAACACTTGAGATGGGGGGCTGCATGCAACCTTCAGAGCAGCCTTTCC
mm4_exon_Abcc5_chr16_20392660 CAGCTGCCTCGACTTCTCTTTCTTGCCCCTGGTAGCCCTCTTGTCTTTTTTCATTTTGGGGGTCAGCTTGGGCGAGTTCT
GTATACTGGAGTGGGAGGAGTCC
mm4_intergenic_5430435G22Rik|Slc26a9_chr1_131739128 ACAGACATGCACAGCCTTCTCCCAACTGACCCCAAACCACAAACAACACCCGCACTGCCCAAGCCAGATGTGGAGACAGG
AC
mm4_intron_B3galt1_chr2_68013352 GTGATCTCGTTGTTGGTCTCTTATTTTGTTTTCTGTTTGGTTTTAATTTTTATCTTGATATGGATGTCTTCTTTGTATTT
CTGAATGCGGTTCAACTGGG
CTAACAGGTCTCCTTACTTAGGCTACTCTGTCAAAGAAGC
mm4_intergenic_AC112986.1|Tsn_chr1_117578699 ACAACCTCTTCATGCACAAGCTTTCTAACATTTCCAAAGTTTCTCTCAGAGAAAAAGGAAAATATCATATTTATGCTGTC
TCTGCATGGGGGCCACCTGGG
CTTTTTCAAGGGCTCAGTGCTGGTCTCTGCTC
mm4_exon_Cda_chr4_138351230 CTGCTATAACTGGCCCACCCCACTCACCTAGAGATGGCAATAGCCCTGAAATCCTTGTACCCTTCGGAGATGGCCTTCTG
GATGGCAGTCCGCTCGG
CACACACACCTAGTGGGTAGCAGGCGTTT
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12494339 CTCAGCTTGACAGGCCTCTCTGACTCTCAAAACCAGAAAGCTCGCCCATATGAACCCCTTCCAGAAACAGCAAAAGCATG
TTAGACTTTCCATAGCCGTCTGTCTTCATGCTTCTTGCTTAGGCCCGTA
mm4_intron_Slc10a7_chr8_78589961 GCCTGTCATTTGAGCTGGGATCTTCTTTCTAGATGGTGCTCAGATAGGTGAACTTAGCAGTCATTTGCTCCCGAGTCCTC
TGCTTCTGTTGCAGATTGCTTTTCCCCTTGATCGACTCCCT
mm4_intergenic_Slc39a8|Bank1_chr3_135971612 TTCCTGAGCTGCCTCCAAAGCTGAAGGTGAGATGCTACACTGAGGCTTCGCAGCTGGGGAATGGAAGCCCACCTGACCCC
AGCCCAGAAA
GGAAGCTCTGACGACTATGAAGAAAACCTGGTCAGTGGCA
mm4_intron_Dapp1_chr3_137961371 ATGCAATGTTCTCAGCCCCAGCAGAAGTCACCAGACTCCAAGCAGATCCTCATGCAGAAATATGTATTAATAACCATATT
AATAAGCCAGGTATTTAGACAGCTTACCTGTCTCGCTTCCA
mm4_intergenic_Mrpl36|Lpcat1_chr13_73336010 TAGCAGGCCAAGACAAAGGGTCAGGGTATCAAGGTATTTGGGGCTACACATGCCACCAAGCTGAGCACCTTCCAGGAATG
AAGAATGAAGGGCTGAAGAGCACAG
mm4_exon_Polr3k_chr2_181869112 GGTCTACAGTAGAGCTTGTGCCCCATTTGAACCCCATCCAAAAATCTATTTTAACCTCAATAAATTTAGAAAAGTTACAT
ATTAAGGCTATGTGAAGAATGAGCTTATATATGGACCAACTTCAGATGAGAGTG
mm4_intron_Synm_chr7_67757850 GACCAACTTCCTGAGAGCCCCTCATCGTGTGATTTGGGAGTAATTCCAATTCTGGCTGGGAGTGAGCTAGGTTGGGAGCC
AAACACTAGGGCCAAACTGAGC
mm4_intergenic_Amer2|Spata13_chr14_60439894 TCACTCAGTGGCAAAGGACCAAAGTCCTTCCCTACACCAGCAAAAGCTCCCCAGGTGAACGCCATCCATAAATGCACCCA
GGAGAGGCGAGATGCCAAGGAGTAGAGAAGCTTAGACCAGGGTCCATCAACCT
mm4_intron_Ttc17_chr2_94375022 TGGGAAGGAAACAGTCTGAGAAAAAACCAAAATGAAAAAAACAAACCAAGCTCACCCCCCTCTAGCAACTGTTCTATCTC
AACAAACAAGAAAGTTGTATTTACTGCATATATATTCCCCAGTGTGTAATAGCTGGT
mm4_intron_Camta1_chr4_151270871 AATTCTGCAGACGGGCTTGTAATTATTTAGCACTCTCCCTCTCTGACCCCCACCCAGATACATTAACTAATGGCCTCAAC
AGCGCATAATG
mm4_intergenic_Gm23694|Igkv7-33_chr6_70035735 AAGCCACTCACATCCTCGTGTCAAAGGAAAGTACTGCAAACTCTTGAACTGTATTTTCCAACCTGACCATTATCCAGAAA
GGCAGGCCAAGAGTTCACTCCA
mm4_intron_Tgm5_chr2_121048304 GGGATGTCCTCTTGAGCAGGCCCAGCTGCCTCCCATCCAGAGCTCAGGGCCCCAGGACCACTTTTGTAAGAGACCACCCT
TTGCCTACATTAATCATGATGCCCGGGAAAGTC
mm4_intergenic_1700080O16Rik|Gpc4_chrX_52017857 ATTCTTGGCAGTCTGGCTCCTCTTCTCTGGCAGGGGGTCAGCCTGGGGCCACTGGGGTCAAGAAACCTCCATGGGCC
mm4_intergenic_Olfr251|Olfr147_chr9_38399664 AGACGATGATATTACCCATGTGGATATATAATTTGTACATGTTTGTTGTCCTATATTGGTAGAATGATATTGTATCTTGA
TGGTGGTCAGGTTGG
TAGCAGTATTTTTAGGATTGACTTCCACAAATCTATATCGAGACC
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45958616 CAGGAGGAGAGGGACCAAGAAGGGGACCTCAGGGGCCAGCTTGAGGCTGGGGGTCAGCTGAGTAGTTCTTTTATAGACCA
CTAGATGTCACTTCCATTTCAAAGGAACCCACTGGCT
mm4_intergenic_Sh3gl2|Gm12413_chr4_85225497 GTGTTGAGCATTGGCAGCTCTGTCTTTTGGGAAGGGGGGCAGCTGGGATATGTGGTACAGAAGGATGCGCCCAGGGCTCG
ATGGGAACTTCCCCTCCTCACCCTTTTGTC
mm4_intron_Sardh_chr2_27242646 TTTACACCCCACCTTTCCCCCTACGGAATACAATCCCCCACCTGAGCCCCCTGCAGAAAGCATGCCCAGCTTTCCCTAGG
GGCACACCCACCGACATGAGCCTCTTGT
mm4_intron_Cyp2f2_chr7_27132058 ACCATTCTTGGAGGACACCCTCTCCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCA
CTCAGAAA
TCCCGCGTCTGCTTCTACAAAGCTCAGGACCAAGGG
mm4_intergenic_Gm20646|Mrps9_chr1_42777228 CCCAGCTTCTACAGAACTCGGTTTTCTGACCTGTGAAAATTGGATAGTTTTCTGGATGTGTGTCCACTGGGCAGTAAGCT
CAAGTTCAGAGCAGTGAAGGTCTACCCTTTCTTCTTCGGCCAT
mm4_intergenic_Gm25722|Gm10051_chr5_132826276 ACACCCAGATTTGGTAGGGCTTTGCCTGGTCTGTGCTGGCTAGGGGTCAGCTGGAGTTGAGTTGGGAAAGAGCTGGCT
mm4_intergenic_Stmn4|Gm23899_chr14_66455265 AGCATAGATACCCCTCTGGCTTTACTCTAGTCATTGCTCAAACATTGTCAAAGGAAAGGTGTTTATTGATGGGGGTCTGC
GTGG
GCAGACAATATGTCCAGGCCTCCAAAGTTC
mm4_intron_Ldlrap1_chr4_134759592 GAGCCTGGGTTCACAGAGAGGGCAGGGGAGAGTTGGGAAGGGACCCACCTGGCCCCCATGCAGGAAAGATTGCTGAGATC
AAGGGGGCTTAGACCCCAACGGAAGGTTTGGCCATGCCCCAATTCCTCCTT
mm3_intron_B4galnt4_chr7_141063761 CGGAAGTGTTCAGCAGTCCTTCTTGCCCCACCCCTCCAGCTGACCCCCAACAACAAAGATAATGGCCTTAAGGAGTCTGA
GCTCTAACACGCCTCATATGCGTCTGCAGATGGGGAGAAG
mm4_exon_Rfx1_chr8_84091361 AGCACCCCACTACCCCTTCCCCTCCAGGCTGAAGCCCATCCAGAAGATGGAGGGCGTGGCCAACGGTGTTGCCGTGGGGC
AGCAGAGCACAGGGCTGTCAGACATCA
mm4_intergenic_Tmem132e|Gm24612_chr11_82453635 TGGCTGCCAGCTCTTCTAACTCCATCCTTCTGTCCTCCTTTCCCAAAGCTGCATCTTAGTCCTCTTGAAGGGGTCTTTCT
GGGGGGGGGTGAGGTGGG
GGAGGGATTTCTGCAGCAATGGC
mm4_intergenic_Gm25183|Spats2_chr15_99147124 ACCTTCTCATTACCTTTCTCCACCTACATGCAGGTTGGGGGTCAGCTAGAGACATTTAAAAAGTTTTGACTCTCTGTGAT
ATCTTTATCTTGAATCGGTGAAACACTTCAAGGTTCC
mm4_intergenic_Tsku|Gucy2d_chr7_98404813 AGACTCCCAGCCCTGTATGTTTCCAGGATCTGTGGGTATCTTGGGGTAAAGAGCCCCTTTGGCTTTGCTGGATGGGGAGT
AGCTAGG
GAGATCTTCTGTGTCATCCAGAGAACACACAATCCCT
mm4_intron_Fhad1_chr4_141920661 CCGAGGGCTTCTAGCATCGTGTGCAATGAGCAGCAGAGGGGCCGACGGAGAACATGGGGGTTTTTGGATGGGGTCCAGCC
TGG
GGCCATGCAGGAACAGTTAGATACGGAGTTTCTTCTGCAGCCCCA
mm4_intron_Pcdh15_chr10_73724816 AGAGTCTTGCTTGTGAGGTGTATATATTTATGATCTTTTTTTGACACATTTATTTTGTGTGTGTGTATTTTTCTGCATGG
GGGTGGGGTGGG
CTCCTCTGCGTCAAGCCATTCATGCAGAGGTGAGAGGACAACGTCTGG
mm4_intergenic_Wnt9b|Wnt3_chr11_103753156 GGGGCTCTTCGGTTTGTCTTCCTCCTTGTCCCACCCTCCTTGCTCCAGGCAGACCACCATCCTGAAACAAAGCCTCAGGA
ACCACCCGTGCCTGGCTCCCTAGCAAGAGCTCACCCAGAAGA
mm4_intron_Camsap2_chr1_136343821 AAGCACACCAGACTCCAGTGACCAGCCTGACACACATCCAGAAAGCAGGGCGTTTCTGCTCAGAAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.