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Contents:

PCR primers for off-targets of CTGAGCTACTAGGTTGCCCT TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314161_F TCGTCGGCAGCGTCGAGCCACGGTCTGCTGGAG 62.6 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314161_R GTCTCGTGGGCTCGGTGCAGCTCACCTCGATTTCT 59.3 1.00
mm3_intergenic_Rab8b|Rps27l_chr9_66937525_F Primer3: not found at this Tm N.d. 0.81
mm3_intergenic_Rab8b|Rps27l_chr9_66937525_R Primer3: not found at this Tm N.d. 0.81
mm3_intergenic_Pvrl3|Gm6912_chr16_46600873_F TCGTCGGCAGCGTCGAGCACAGTCATCTTGGCCT 60.0 0.46
mm3_intergenic_Pvrl3|Gm6912_chr16_46600873_R GTCTCGTGGGCTCGGAGCCAGACAGACAATTGATTTCT 58.3 0.46
mm4_intergenic_Gm8349|Arhgef26_chr3_61536497_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Gm8349|Arhgef26_chr3_61536497_R Primer3: not found at this Tm N.d. 0.45
mm4_intron_Nhs_chrX_161870873_F Primer3: not found at this Tm N.d. 0.37
mm4_intron_Nhs_chrX_161870873_R Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Wdr1|4930421P07Rik_chr5_38566305_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Wdr1|4930421P07Rik_chr5_38566305_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Spin2-ps5|Gm21699_chrX_34310515_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Spin2-ps5|Gm21699_chrX_34310515_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Spin2-ps1|Gm3701_chrX_3930348_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Spin2-ps1|Gm3701_chrX_3930348_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gmcl1l|Gm5926_chrX_32588059_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gmcl1l|Gm5926_chrX_32588059_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm3763|Gm14370_chrX_5112288_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm3763|Gm14370_chrX_5112288_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm3750|Gm4726_chrX_4827035_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm3750|Gm4726_chrX_4827035_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2933|Gm2964_chrX_33856587_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2933|Gm2964_chrX_33856587_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2927|Gm2933_chrX_33682724_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2927|Gm2933_chrX_33682724_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2863|Gm2182_chrX_33340053_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2863|Gm2182_chrX_33340053_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2825|Spin2-ps6_chrX_33000640_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2825|Spin2-ps6_chrX_33000640_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2799|Gmcl1l_chrX_32305185_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2799|Gmcl1l_chrX_32305185_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2231|Gm2913_chrX_33543836_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm2231|Gm2913_chrX_33543836_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21870|Gm21681_chrX_34134804_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21870|Gm21681_chrX_34134804_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21699|Gm2288_chrX_34546347_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21699|Gm2288_chrX_34546347_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21447|Spin2f_chrX_31197966_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm21447|Spin2f_chrX_31197966_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm15276|Gm2777_chrX_31357119_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm15276|Gm2777_chrX_31357119_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm15250|Gm15258_chrX_4064454_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm15250|Gm15258_chrX_4064454_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14347|Gm10921_chrX_4264454_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14347|Gm10921_chrX_4264454_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14345|Gm14351_chrX_3732108_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14345|Gm14351_chrX_3732108_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14334|Gm14352_chrX_3348376_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm14334|Gm14352_chrX_3348376_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm10922|Gm6019_chrX_4397338_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm10922|Gm6019_chrX_4397338_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm26455|Gm15754_chr5_118433722_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm26455|Gm15754_chr5_118433722_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm26181|Triml1_chr8_42809893_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm26181|Triml1_chr8_42809893_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Elmo1_chr13_20117917_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Elmo1_chr13_20117917_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Rap1gap_chr4_137711941_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Rap1gap_chr4_137711941_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm5611|Chordc1_chr9_17712166_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm5611|Chordc1_chr9_17712166_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm14365|Gm9428_chrX_5257328_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm14365|Gm9428_chrX_5257328_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm14467|Gm17014_chr2_78837944_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm14467|Gm17014_chr2_78837944_R Primer3: not found at this Tm N.d. 0.18
mm3_intron_Msi2_chr11_88681887_F Primer3: not found at this Tm N.d. 0.17
mm3_intron_Msi2_chr11_88681887_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Asph_chr4_9519483_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Asph_chr4_9519483_R Primer3: not found at this Tm N.d. 0.15
mm4_exon_Bag4/Gm17484_chr8_25765934_F Primer3: not found at this Tm N.d. 0.14
mm4_exon_Bag4/Gm17484_chr8_25765934_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Josd2|Emc10_chr7_44489078_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Josd2|Emc10_chr7_44489078_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Stc2|Bod1_chr11_31380140_F TCGTCGGCAGCGTCGATCAGAGCAGGAGCAGTGG 60.1 0.14
mm4_intergenic_Stc2|Bod1_chr11_31380140_R GTCTCGTGGGCTCGGGGAAAGGCAGCAACCCATTG 60.0 0.14
mm4_intergenic_Gm25292|Gm26667_chr16_84695560_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm25292|Gm26667_chr16_84695560_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Gm13364_chr2_15677089_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Gm13364_chr2_15677089_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_4930523C07Rik_chr1_160048536_F TCGTCGGCAGCGTCAAGGACAGTGACGAGAGGGA 59.8 0.14
mm4_intron_4930523C07Rik_chr1_160048536_R GTCTCGTGGGCTCGGAGCACTGGACAATGTTGGGA 59.5 0.14
mm4_intron_Col18a1_chr10_77119205_F TCGTCGGCAGCGTCATCCCGGCTACCTGGTTACT 60.0 0.13
mm4_intron_Col18a1_chr10_77119205_R GTCTCGTGGGCTCGGCGGGAGTTTAGAGGCCTGTG 60.1 0.13
mm4_intron_Tmem132d_chr5_128219436_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Tmem132d_chr5_128219436_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Peli2|Gm6498_chr14_48190376_F TCGTCGGCAGCGTCTTGTGTTGGTAGGCTCCACC 59.8 0.12
mm4_intergenic_Peli2|Gm6498_chr14_48190376_R GTCTCGTGGGCTCGGATTTCCCTTGTTGCCTCCCC 60.2 0.12
mm4_intergenic_Gstm5|Gm12489_chr3_107901931_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gstm5|Gm12489_chr3_107901931_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm22828|Gm23960_chr6_20006874_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22828|Gm23960_chr6_20006874_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Kctd14_chr7_97457350_F TCGTCGGCAGCGTCAGGACTGTTGCCATGGCTAC 60.0 0.11
mm4_intron_Kctd14_chr7_97457350_R GTCTCGTGGGCTCGGTGTGGAGCCCTCATCTCTCA 59.9 0.11
mm3_exon_Tonsl_chr15_76630490_F TCGTCGGCAGCGTCGAATCTTCTCAACCCCGGGG 60.1 0.10
mm3_exon_Tonsl_chr15_76630490_R GTCTCGTGGGCTCGGTGTGGCTTCAGCTCCAGTTT 59.8 0.10
mm4_intergenic_5330434G04Rik|Rab9b-ps1_chrX_105403834_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_5330434G04Rik|Rab9b-ps1_chrX_105403834_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm14263|Gm14266_chr2_171017400_F TCGTCGGCAGCGTCTCACGAGCATAGAGGAGACCA 60.0 0.10
mm4_intergenic_Gm14263|Gm14266_chr2_171017400_R GTCTCGTGGGCTCGGTGCAGCTGGTAGTGGTCTTG 59.9 0.10
mm4_intergenic_2810407A14Rik|Grik1_chr16_87858367_F TCGTCGGCAGCGTCAAAGTATGCTGAACCGGCCA 59.9 0.10
mm4_intergenic_2810407A14Rik|Grik1_chr16_87858367_R GTCTCGTGGGCTCGGTAGGAAGCAGTGCACACAGC 60.6 0.10
mm4_intergenic_Gm9621|Gm24200_chr9_94082373_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm9621|Gm24200_chr9_94082373_R Primer3: not found at this Tm N.d. 0.10
mm3_intergenic_Homer1|Jmy_chr13_93421032_F TCGTCGGCAGCGTCTGTAGAGACGGGGCAAACAC 59.9 0.10
mm3_intergenic_Homer1|Jmy_chr13_93421032_R GTCTCGTGGGCTCGGATGGGAGCCTGGTTTTGAGG 59.9 0.10
mm4_intergenic_Ntrk2|Agtpbp1_chr13_59314131_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Ntrk2|Agtpbp1_chr13_59314131_R Primer3: not found at this Tm N.d. 0.09
mm3_intergenic_Ahcy|2310005A03Rik_chr2_155082519_F TCGTCGGCAGCGTCCTAGGAAGGAGGAGCCCAGT 60.0 0.09
mm3_intergenic_Ahcy|2310005A03Rik_chr2_155082519_R GTCTCGTGGGCTCGGACCTCGGGCTGAAGTCAAAG 59.9 0.09
mm3_intron_Kalrn_chr16_34430997_F TCGTCGGCAGCGTCAAAAGGGTGATGCCACGAGT 59.8 0.09
mm3_intron_Kalrn_chr16_34430997_R GTCTCGTGGGCTCGGCCAGGGATGGGGAAAAGAGG 59.7 0.09
mm4_intergenic_Rbbp8|Gm6277_chr18_11813879_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Rbbp8|Gm6277_chr18_11813879_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Obscn|Iba57_chr11_59138636_F TCGTCGGCAGCGTCCCCCATGCCCTTCTAGAACG 60.1 0.08
mm4_intergenic_Obscn|Iba57_chr11_59138636_R GTCTCGTGGGCTCGGGACAGCCAGAGGGGTACTGA 60.6 0.08
mm4_intergenic_Gm22199|Spin1_chr13_51091441_F TCGTCGGCAGCGTCTGGCTGGGGATCGATTTGAA 59.3 0.08
mm4_intergenic_Gm22199|Spin1_chr13_51091441_R GTCTCGTGGGCTCGGGGGTGGAAAAGTTGGTTCAGG 59.3 0.08
mm4_intron_Srpk2_chr5_23574499_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Srpk2_chr5_23574499_R Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Smoc1|Mir3067_chr12_81160595_F TCGTCGGCAGCGTCTAAAGCCCAGGCCTTCCTTG 59.9 0.07
mm3_intergenic_Smoc1|Mir3067_chr12_81160595_R GTCTCGTGGGCTCGGCACAGGCTTCTCCAGGTCTG 60.0 0.07
mm4_intron_Il13ra1_chrX_36123469_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Il13ra1_chrX_36123469_R Primer3: not found at this Tm N.d. 0.07
mm3_intron_Capn8_chr1_182587799_F TCGTCGGCAGCGTCTGGAGACACCAAAGGACAGAG 59.3 0.07
mm3_intron_Capn8_chr1_182587799_R GTCTCGTGGGCTCGGCCTGGGCTCTCCTCTAAGGT 60.0 0.07
mm4_intergenic_Gm12071|Gm12070_chr11_26754867_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12071|Gm12070_chr11_26754867_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_March3_chr18_56870655_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_March3_chr18_56870655_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13375|Gm13377_chr2_21035948_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13375|Gm13377_chr2_21035948_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Tshz2|AY702102_chr2_169928955_F TCGTCGGCAGCGTCGCCACGGGAGTTCATTACCA 60.0 0.06
mm4_intergenic_Tshz2|AY702102_chr2_169928955_R GTCTCGTGGGCTCGGCATACGAGGCCAAGCGGTAT 59.9 0.06
mm4_exon_Hjurp_chr1_88275465_F TCGTCGGCAGCGTCGGGGAGACACGTAGCATACA 58.8 0.06
mm4_exon_Hjurp_chr1_88275465_R GTCTCGTGGGCTCGGCGCTCCTTCAAGCTCCAGAG 60.4 0.06
mm4_intron_Magix_chrX_7679839_F TCGTCGGCAGCGTCCCGGGTGGAATCACACTCTC 60.1 0.06
mm4_intron_Magix_chrX_7679839_R GTCTCGTGGGCTCGGCCACACGGAGGGTAGGTACA 60.6 0.06
mm3_intergenic_Gm12155|Il12b_chr11_44389938_F TCGTCGGCAGCGTCCACATTGGTCATCTGCAAGGC 60.1 0.05
mm3_intergenic_Gm12155|Il12b_chr11_44389938_R GTCTCGTGGGCTCGGTGCAGGCACAAAGACAATGC 59.9 0.05
mm4_exon_Pced1b_chr15_97384931_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Pced1b_chr15_97384931_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Kif26a_chr12_112168762_F TCGTCGGCAGCGTCCCTAAGCACCCTCCTCTCCT 60.0 0.04
mm4_exon_Kif26a_chr12_112168762_R GTCTCGTGGGCTCGGGAAGTCGGTGGGGTAAGTGG 60.0 0.04
mm4_intergenic_Eva1a|Tacr1_chr6_82193194_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Eva1a|Tacr1_chr6_82193194_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ptprn2_chr12_116967810_F TCGTCGGCAGCGTCAGAGCCAAGGTGGAAACTGC 60.5 0.04
mm4_intron_Ptprn2_chr12_116967810_R GTCTCGTGGGCTCGGGCACATGAGGATGCTGGGAA 60.3 0.04
mm4_intergenic_Gm23669|Gm26791_chr14_118301384_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23669|Gm26791_chr14_118301384_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Nrg3|Gm10713_chr14_39700733_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Nrg3|Gm10713_chr14_39700733_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tenm4|Rps11-ps5_chr7_96407310_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tenm4|Rps11-ps5_chr7_96407310_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Nr2f6_chr8_71381610_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Nr2f6_chr8_71381610_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26688|Gm6245_chr13_40759826_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26688|Gm6245_chr13_40759826_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gtpbp1_chr15_79712323_F TCGTCGGCAGCGTCGGGTGGACTGCTTCAGAGAC 60.0 0.02
mm4_intron_Gtpbp1_chr15_79712323_R GTCTCGTGGGCTCGGAGAAGCAGCAGCAGACTCTG 60.0 0.02
mm4_intergenic_Mir5133|Gm25603_chr9_62152720_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Mir5133|Gm25603_chr9_62152720_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Dst_chr1_34137451_F TCGTCGGCAGCGTCTGCTGGACCTCTTATTGGATCT 58.6 0.02
mm3_intron_Dst_chr1_34137451_R GTCTCGTGGGCTCGGCAGCCAGATAGCACCACCTC 60.1 0.02
mm4_intron_Trpv4_chr5_114656371_F TCGTCGGCAGCGTCCTGCGATTGTGTCCGCTCTA 60.1 0.02
mm4_intron_Trpv4_chr5_114656371_R GTCTCGTGGGCTCGGGTCCAGAGTTGTGTGAGCCA 59.8 0.02
mm4_intron_Acan_chr7_79060703_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Acan_chr7_79060703_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmpo|Rmst_chr10_91582151_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmpo|Rmst_chr10_91582151_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Dmkn_chr7_30778532_F TCGTCGGCAGCGTCGCATTGCTCTTGCTTGCAGT 60.0 0.01
mm4_intron_Dmkn_chr7_30778532_R GTCTCGTGGGCTCGGAGCAAGGAGGCCTTCAGTTC 59.9 0.01
mm4_intergenic_Jag1|Gm14055_chr2_137190177_F TCGTCGGCAGCGTCTGGTGTGTGTGTCTGTGGAC 60.1 0.01
mm4_intergenic_Jag1|Gm14055_chr2_137190177_R GTCTCGTGGGCTCGGGATTTTGATGCTGGGGCTGG 59.5 0.01
mm4_intergenic_Eif5al3-ps|Cox18_chr5_90135572_F TCGTCGGCAGCGTCGGACCCCTCAGTTATCTGCC 59.5 0.01
mm4_intergenic_Eif5al3-ps|Cox18_chr5_90135572_R GTCTCGTGGGCTCGGCCCGGGATATGGGTAAAGCC 59.9 0.01
mm2_intergenic_Sept2|Farp2_chr1_93511129_F Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Sept2|Farp2_chr1_93511129_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Odf3l2|Madcam1_chr10_79655697_F TCGTCGGCAGCGTCCTCGTATGGGTGGGGTGAAG 59.8 0.01
mm4_intergenic_Odf3l2|Madcam1_chr10_79655697_R GTCTCGTGGGCTCGGGGCTGCACTTAAGGACTCCA 59.6 0.01
mm4_exon_Evpl_chr11_116220866_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Evpl_chr11_116220866_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Syce1l_chr8_113655130_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Syce1l_chr8_113655130_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm46|Serpina1f_chr12_103676607_F TCGTCGGCAGCGTCTCTCCAATCATGCAGAGGGC 59.8 0.01
mm4_intergenic_Gm46|Serpina1f_chr12_103676607_R GTCTCGTGGGCTCGGCTTCTGTCCTGGGCAGCTAC 60.1 0.01
mm4_intergenic_Kcnh8|Efhb_chr17_53290826_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Kcnh8|Efhb_chr17_53290826_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Trappc8|Rnf125_chr18_20902249_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Trappc8|Rnf125_chr18_20902249_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Lphn1_chr8_83931690_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Lphn1_chr8_83931690_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp541_chr7_16092273_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp541_chr7_16092273_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pvt1|Gm24810_chr15_62463399_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pvt1|Gm24810_chr15_62463399_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm24444|Mgmt_chr7_136877919_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm24444|Mgmt_chr7_136877919_R Primer3: not found at this Tm N.d. 0.00
mm2_intergenic_Larp4b|Dip2c_chr13_9193754_F Primer3: not found at this Tm N.d. 0.00
mm2_intergenic_Larp4b|Dip2c_chr13_9193754_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fyn|Gm16365_chr10_39572835_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fyn|Gm16365_chr10_39572835_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Itpr3|Itpr3/1700062I23Rik_chr17_27099159_F TCGTCGGCAGCGTCTATGAGGAGCCTGGTGGTGA 59.9 0.00
mm4_intergenic_Itpr3|Itpr3/1700062I23Rik_chr17_27099159_R GTCTCGTGGGCTCGGGAGCCTGACAGCCCTTATCC 59.8 0.00
mm4_intergenic_Ankrd1|Pcgf5_chr19_36261999_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ankrd1|Pcgf5_chr19_36261999_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tex15_chr8_33557105_F TCGTCGGCAGCGTCTTAGTCCTAACTCACCGGCA 58.0 0.00
mm4_intron_Tex15_chr8_33557105_R GTCTCGTGGGCTCGGCACGCATCTCTGACCTACGT 59.5 0.00
mm4_intron_Stab2_chr10_86858267_F TCGTCGGCAGCGTCGAAAGAGGCCTCCCTTCACC 60.0 0.00
mm4_intron_Stab2_chr10_86858267_R GTCTCGTGGGCTCGGAGGGTGCTTAGCAATCACCC 60.0 0.00
mm4_intron_Samd4_chr14_47066693_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Samd4_chr14_47066693_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Park2_chr17_11863549_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Park2_chr17_11863549_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fmn2_chr1_174663368_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fmn2_chr1_174663368_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Adamts18_chr8_113796531_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Adamts18_chr8_113796531_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r160|Gm4214_chr7_22891070_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r160|Gm4214_chr7_22891070_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r123|Vmn1r124_chr7_21182039_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r123|Vmn1r124_chr7_21182039_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r118|Vmn1r119_chr7_20936636_F TCGTCGGCAGCGTCCCAACCCTGGAGGAGGAAAC 59.9 0.00
mm4_intergenic_Vmn1r118|Vmn1r119_chr7_20936636_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Vmn1r111|Gm10668_chr7_20722250_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r111|Gm10668_chr7_20722250_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r-ps77|Gm5891_chr7_21907154_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r-ps77|Gm5891_chr7_21907154_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r-ps75|Gm6882_chr7_21424344_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vmn1r-ps75|Gm6882_chr7_21424344_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tiparp|mmu-mir-8120_chr3_65601760_F TCGTCGGCAGCGTCTCTTCGGACAAGTGCAGACC 59.9 0.00
mm4_intergenic_Tiparp|mmu-mir-8120_chr3_65601760_R GTCTCGTGGGCTCGGACTGAATGAAGCAGGCTTTGG 59.1 0.00
mm4_intergenic_Snx29|Cpped1_chr16_11759559_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Snx29|Cpped1_chr16_11759559_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rpsa-ps10|Mir3963_chr3_150107598_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rpsa-ps10|Mir3963_chr3_150107598_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rell1|Gm15819_chr5_63917666_F TCGTCGGCAGCGTCAACCCTGGCTCCCCATCTAT 60.0 0.00
mm4_intergenic_Rell1|Gm15819_chr5_63917666_R GTCTCGTGGGCTCGGAGGCCAAAGTCCAGTGAAGG 59.8 0.00
mm4_intergenic_Mtx3|Cmya5_chr13_93035921_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mtx3|Cmya5_chr13_93035921_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Jph4|Gm23596_chr14_55154416_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Jph4|Gm23596_chr14_55154416_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8693|Gm4567_chr7_22711336_F TCGTCGGCAGCGTCCAAACCAACCCTGGAGGAGG 60.2 0.00
mm4_intergenic_Gm8693|Gm4567_chr7_22711336_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm8453|Gm4558_chr7_22253770_F TCGTCGGCAGCGTCCCAACCCTGGAGGAGGAAAC 59.9 0.00
mm4_intergenic_Gm8453|Gm4558_chr7_22253770_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm8215|Gm5390_chrX_62091229_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8215|Gm5390_chrX_62091229_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4498|Gm4133_chr7_20258112_F TCGTCGGCAGCGTCCCAACCCTGGAGGAGGAAAC 59.9 0.00
mm4_intergenic_Gm4498|Gm4133_chr7_20258112_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm4220|Gm6902_chr7_23270526_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4220|Gm6902_chr7_23270526_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4201|Vmn1r155_chr7_22579814_F TCGTCGGCAGCGTCCAAACCAACCCTGGAGGAGG 60.2 0.00
mm4_intergenic_Gm4201|Vmn1r155_chr7_22579814_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm4179|Gm6176_chr7_22047930_F TCGTCGGCAGCGTCCAAACCAACCCTGGAGGAGG 60.2 0.00
mm4_intergenic_Gm4179|Gm6176_chr7_22047930_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm4172|Vmn1r132_chr7_21565122_F TCGTCGGCAGCGTCCAAACCAACCCTGGAGGAGG 60.2 0.00
mm4_intergenic_Gm4172|Vmn1r132_chr7_21565122_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm4141|Vmn1r107_chr7_20590707_F TCGTCGGCAGCGTCCAAACCAACCCTGGAGGAGG 60.2 0.00
mm4_intergenic_Gm4141|Vmn1r107_chr7_20590707_R GTCTCGTGGGCTCGGCTCAGCAGGACATCCCAGTC 59.8 0.00
mm4_intergenic_Gm26562|Cpn2_chr16_30237776_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26562|Cpn2_chr16_30237776_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21989|Asap2_chr12_21089089_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21989|Asap2_chr12_21089089_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16456|Gm10662_chr7_23130343_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16456|Gm10662_chr7_23130343_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Arrdc2|Kcnn1_chr8_70841052_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Arrdc2|Kcnn1_chr8_70841052_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Akap11|Dgkh_chr14_78549898_F TCGTCGGCAGCGTCCAGGGGAGGGTAAACTGAAACT 59.6 0.00
mm4_intergenic_Akap11|Dgkh_chr14_78549898_R GTCTCGTGGGCTCGGATATGATGGGTACTTTCGTTTACAC 57.2 0.00
mm4_intergenic_AL596090.1|Gm12266_chr11_60304660_F TCGTCGGCAGCGTCGAGGATCCCTTCAGAAACGGT 59.4 0.00
mm4_intergenic_AL596090.1|Gm12266_chr11_60304660_R GTCTCGTGGGCTCGGGCCCAGTATCCCAGTAACCC 59.5 0.00
mm4_intergenic_5830416P10Rik|4833407H14Rik_chr19_53456855_F TCGTCGGCAGCGTCGGCCACGTGACTCTATTCCA 59.4 0.00
mm4_intergenic_5830416P10Rik|4833407H14Rik_chr19_53456855_R GTCTCGTGGGCTCGGGTAGCAGCAGAAGGGCCC 60.1 0.00
mm3_intron_Adamtsl3_chr7_82543763_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Adamtsl3_chr7_82543763_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314161 GAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCA
ACCTAGTAGCTCAG
ACACCGAATCGGATTTCTATGAAGAAATCGAGGTGAGCTGCA
mm3_intergenic_Pvrl3|Gm6912_chr16_46600873 GAGCACAGTCATCTTGGCCTTCCAAAAACATCTGTATCCTAGTGCAACCTAGTAGTTCATAGGCTATTTAAATCTTGCAG
TTAATAGGAATCATAGTCAAATTTTGTCCAAAAGAAATCAATTGTCTGTCTGGCT
mm4_intergenic_Stc2|Bod1_chr11_31380140 GATCAGAGCAGGAGCAGTGGTTGTGGCCATTCTTTGCTGGGCAACTAAGCTGCCCTGGGTACTGTGTCACACTGTCAGAA
GCAGGGAGCAGGGTCAATGGGTTGCTGCCTTTCC
mm4_intron_4930523C07Rik_chr1_160048536 AAGGACAGTGACGAGAGGGACCTTTCCCCTCTCTAAGCAGCAAAGGAACAATCTACAGCAGACTCTCTGCAGGACAATCC
CTAGGGCTACCTGCCAGCTCAG
GTGTCTTTTCCCAACATTGTCCAGTGCT
mm4_intron_Col18a1_chr10_77119205 ATCCCGGCTACCTGGTTACTTCCACCCTCCTCGGGTGCCCTAGGGCATCCCCGTGGCTCAGCCAAGCACAGGCCTCTAAA
CTCCCG
mm4_intergenic_Peli2|Gm6498_chr14_48190376 TTGTGTTGGTAGGCTCCACCTAAAGCCATTGTTTGCATTGCCTGCAGGTGCAGTAAATCATTGCTTTTGCACTGCAAGCT
TCTCCCAGGGCTGCCTAGTTGCTCTGGGTTTATGGGGAGGCAACAAGGGAAAT
mm4_intron_Kctd14_chr7_97457350 AGGACTGTTGCCATGGCTACCCTGCTTTCCTGAGCAACTTAGTTGCCTTTGGCTCTCTAACTCCAGTACTGTACTTATGT
CTTAAGAGGCAGTACCCAGACAGGTTGAGAGATGAGGGCTCCACA
mm3_exon_Tonsl_chr15_76630490 GAATCTTCTCAACCCCGGGGCCAGGGAATACACTTAACTGCTCATCTTACCTTGGAAGGCACCGCCCAGGGCAGCCTGGT
GGCTCAG
GAAGAAACTGGAGCTGAAGCCACA
mm4_intergenic_Gm14263|Gm14266_chr2_171017400 TCACGAGCATAGAGGAGACCAGACAAGGATTCCATAAGTGGTCCCATGAGCAACCAAGTATCTCAGCAAGACCACTACCA
GCTGCA
mm4_intergenic_2810407A14Rik|Grik1_chr16_87858367 AAAGTATGCTGAACCGGCCACCTTGGTTTGGATGGTTGGTCTCTCAAATACACTCTGAGCATCTGGCTTGCCCTTGGAGG
CTGTGTGCACTGCTTCCTA
mm3_intergenic_Homer1|Jmy_chr13_93421032 TGTAGAGACGGGGCAAACACCTGAGGTCCTAGGTTGTCCTGGGGCTGCCTTTCCTCTACCTGGCTTGAACTTTACCATGC
CTCAAAACCAGGCTCCCAT
mm3_intergenic_Ahcy|2310005A03Rik_chr2_155082519 CTAGGAAGGAGGAGCCCAGTCCTAAGGGCAACCTTGTACCTCTGAGGTTAGCCTTAGCCAGGCTGGAGTCGCCTTTCGCT
GACTCTGCCAGGCGGGGGCACCCTGCACCCAGCTTTGACTTCAGCCCGAGGT
mm3_intron_Kalrn_chr16_34430997 AAAAGGGTGATGCCACGAGTGGGCAGGGAGGCACACTGCCACCACTGGGGTGCTGGTGTGACAGGGAGCTGGGGGCTGGT
ACTAAGCTCCTAGGCTGCCCTGGGCTTCTGCAGAACCTCTTTTCCCCATCCCTGG
mm4_intergenic_Obscn|Iba57_chr11_59138636 CCCCATGCCCTTCTAGAACGAATTTCCAAGGGCAACCCCCGAGCTCAGCCTCACCTTAGACCCCGGGCCTGTGACTTCCC
CCACTCCCGGGAAATTCTTTCCTGGAGTCAAGTGTCAGTACCCCTCTGGCTGTC
mm4_intergenic_Gm22199|Spin1_chr13_51091441 TGGCTGGGGATCGATTTGAAAATCTAAGGATTCCATCTACAGCTTCTAAACATTCTTCAGTAGACCCAGAGCAACCGAGT
AGCAAAG
AGACTTTATCGTTGGTAGATAAACTCCTGAACCAACTTTTCCACCC
mm3_intergenic_Smoc1|Mir3067_chr12_81160595 TAAAGCCCAGGCCTTCCTTGTTGTCTGTCCTTTTCCTGCAGTCTGCCTCTTCTCCAGATTCTCAGCCACTAGGCTGCCCT
GGG
GTAGCCCCAGTACCAGACACCCATGTGCTCTCAGACCTGGAGAAGCCTGTG
mm3_intron_Capn8_chr1_182587799 TGGAGACACCAAAGGACAGAGCTGAGCTACCAGGCTGACCTGGGGAGAGGGCAAAGGTGAAGGGTGACCTTAGAGGAGAG
CCCAGG
mm4_intergenic_Tshz2|AY702102_chr2_169928955 GCCACGGGAGTTCATTACCATAGCGACTGAGCTGGCAGGCTGCCCTGGGGAACCCACAAGTGCCAGCTTCCACCTTCCTT
GTGTAGATCATGGAAGTCCTGACCGGATACCGCTTGGCCTCGTATG
mm4_exon_Hjurp_chr1_88275465 GGGGAGACACGTAGCATACATTTAACCTCATCTTGGGAGTCAGTGCTTTCCTAGGGCGATCTAGAAGCCCAGGACAGAGA
AGCCCACGTACAGAGAACTCTGGAGCTTGAAGGAGCG
mm4_intron_Magix_chrX_7679839 CCGGGTGGAATCACACTCTCTAACAGTCCCAGGGCTAGCTAGAAGCCCAGCCCCTCCTGCCCCGCCCTACCAAGTCCTTG
TACCTACCCTCCGTGTGG
mm3_intergenic_Gm12155|Il12b_chr11_44389938 CACATTGGTCATCTGCAAGGCAGATTGTCTTAAGGCAACCTGGTAGCTCACCCTCATTCAGGGCATGATCAGAGCATTGT
CTTTGTGCCTGCA
mm4_exon_Kif26a_chr12_112168762 CCTAAGCACCCTCCTCTCCTCCCCAGGGCTGCCCAGAAGCTCAGCCTGGCCTCCAAGCGCAAGAAGCATCACCCGCCGCC
TGCGCCCTCCACCCGAGGCACATCCACTTACCCCACCGACTTC
mm4_intron_Ptprn2_chr12_116967810 AGAGCCAAGGTGGAAACTGCCTTAGCATACTCCTTGTCTGTAATTTAGAACAGCTCTATTCAGTAACAACCAGAGAACCA
GCTAGCTGAGCCTCTCTGTTGCCCTGGGGAATGTCAGTGTTTCCCAGCATCCTCATGTGC
mm4_intron_Gtpbp1_chr15_79712323 GGGTGGACTGCTTCAGAGACTCAGAGAGCACCTTCTGCATCCTGTCTGTGCTAGTAGGTTAGCCTCGGGGTCCATCAGAC
AGAGTCTGCTGCTGCTTCT
mm3_intron_Dst_chr1_34137451 TGCTGGACCTCTTATTGGATCTCATTTGGCTTCAGTAATGTTATCTGAGCTAGTAGGTAGGCCTGGGTTCTAAGCAAATG
AGGACCTTTAGAGGTGGTGCTATCTGGCTG
mm4_intron_Trpv4_chr5_114656371 CTGCGATTGTGTCCGCTCTACAGCTAAAAGGATCTTGCTATACCACACAAACAATGGTGGCATCTCCCCCTTCCCACCCA
CCCCCCAACTTTCAGAGCTTCTAAGTTGGCCTTGGCCTGGCTCACACAACTCTGGAC
mm4_intron_Dmkn_chr7_30778532 GCATTGCTCTTGCTTGCAGTTGTGGGGCAGCAGCTGGAGTAGAGGATAGCATCCCAGCGCATCCTAGAAGCTAAGCTTTG
GGACTTTTGGTTGGTGGGGAACTGAAGGCCTCCTTGCT
mm4_intergenic_Jag1|Gm14055_chr2_137190177 TGGTGTGTGTGTCTGTGGACTTTGCTGAGATACTAGGTTGGACAGGGACACCCTACTCTCTCCAAAGCCCCAGCCCCAGC
ATCAAAATC
mm4_intergenic_Eif5al3-ps|Cox18_chr5_90135572 GGACCCCTCAGTTATCTGCCTTACCCACATCCCAGGGCCTCCTAGTATCTAAGCCTTTCCTATACCCTAGGGCCACCTAG
CACTTAGGCCTTACCAATACTTCAGAGCCTCAATGACCCAGGCTTTACCCATATCCCGGG
mm4_intergenic_Odf3l2|Madcam1_chr10_79655697 CTCGTATGGGTGGGGTGAAGCATGGAGATGTGGAAGGGAGGGTACTGAGCCTATAGGTGGCCCTTGGTGGCTGGGGAGGG
CTAGAGAAAGGCAATCTCTGAGGGTAGGATGTGGAGTCCTTAAGTGCAGCC
mm4_intergenic_Gm46|Serpina1f_chr12_103676607 TCTCCAATCATGCAGAGGGCCATATCTCCAGGGGCAAACTAGGAGCTCTGGGGCCCAGGCCCCAGGCCAGAAGAAAGTAA
GTTTTACTTACAGGACATATACTGTGAGTCTGACTCACCTGTAGCTGCCCAGGACAGAAG
mm4_intergenic_Itpr3|Itpr3/1700062I23Rik_chr17_27099159 TATGAGGAGCCTGGTGGTGAGGCTTGGCCCCTCCTCAGTTCCTTCCTGGCCCCGCCTCAGCCCCCTCCAACCTAGTAGCT
CAG
GGGCTGGGAAGCCCTCGAGTCTGGCTTGTCTTAAAGGATAAGGGCTGTCAGGCTC
mm4_intron_Tex15_chr8_33557105 TTAGTCCTAACTCACCGGCATCCTAGTGGAAGCTAGTAGCTCTGATTATTGTCAGTAGTGTGTAATTTCTTTGATAAGCT
GAAGTAGTGTAACTGTATGTCATTTGTTTTTACGTAGGTCAGAGATGCGTG
mm4_intron_Stab2_chr10_86858267 GAAAGAGGCCTCCCTTCACCCTGCGCCCCACTGTGAAGAGTCAGCCTCACCTACTAGGTTTCCCCAGGACCCTTCAGGAG
ATTCATAAATGGCAGAGAGAGGGGGTGATTGCTAAGCACCCT
mm4_intergenic_Vmn1r118|Vmn1r119_chr7_20936636 CCAACCCTGGAGGAGGAAACTGATTTGGGAACAGGGTAGAGATTAGGAACATGTGTCCCAGAAAAGGACTGATTTTCTTG
TGCAAACAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Tiparp|mmu-mir-8120_chr3_65601760 TCTTCGGACAAGTGCAGACCAACGGCCCCATCATAATGCTTATTGCTGGTGTCAGGCCGAGTCTGGCATCTCCCTAGTGA
AACTAAGTAGCTCAG
TGAGAATGAGACATTATCCAAAGCCTGCTTCATTCAGT
mm4_intergenic_Rell1|Gm15819_chr5_63917666 AACCCTGGCTCCCCATCTATAAAATGGGGATGTTAGTGTTTCCCACAACTGCCCAGAGCACCCTGGTAACTCAGACCTTC
ACTGGACTTTGGCCT
mm4_intergenic_Gm8693|Gm4567_chr7_22711336 CAAACCAACCCTGGAGGAGGAAGCTGATTTGGGAACAGGTTAGAGATTAGGAACATGTGTCCCAGAAAGGACTGATCTTC
TTGTGCAAGCAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm8453|Gm4558_chr7_22253770 CCAACCCTGGAGGAGGAAACTGATTTGGGAACAGGGTAGAGATTAGGAACATGTGTCCCAGAAAAGGACTGATTTTCTTG
TGCAAACAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm4498|Gm4133_chr7_20258112 CCAACCCTGGAGGAGGAAACTGATTTGGGAACAGGGTAGAGATTAGGAACATGTGTCCCAGAAAAGGACTGATTTTCTTG
TGCAAACAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm4201|Vmn1r155_chr7_22579814 CAAACCAACCCTGGAGGAGGAAGCTGATTTGGGAACAGGTTAGAGATTAGGAACATGTGTCACAGAAAGGACTGATCTTC
TTGTGCAAGCAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm4179|Gm6176_chr7_22047930 CAAACCAACCCTGGAGGAGGAAGCTGATTTGGGAACAGGTTAGAGATTAGGAACATGTGTCCCAGAAAGGACTGATCTTC
TTGTGCAAGCAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm4172|Vmn1r132_chr7_21565122 CAAACCAACCCTGGAGGAGGAAGCTGATTTGGGAACAGGTTAGAGATTAGGAACATGTGTCCCAGAAAGGACTGATCTTC
TTGTGCAAGCAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Gm4141|Vmn1r107_chr7_20590707 CAAACCAACCCTGGAGGAGGAAGCTGATTTGGGAACAGGTTAGAGATTAGGAACATGTGTCACAGAAAGGACTGATCTTC
TTGTGCAAGCAGCCCAAGGGGAACCTGGAAGCTCTGGGGGACTGGGATGTCCTGCTGAG
mm4_intergenic_Akap11|Dgkh_chr14_78549898 CAGGGGAGGGTAAACTGAAACTAAGTATATTTGAAAGAGCCCTAGAGGAACCTAGTAGGTAAGTTANNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAAACGAAAGTACCCATCATAT
mm4_intergenic_AL596090.1|Gm12266_chr11_60304660 GAGGATCCCTTCAGAAACGGTTTGACTATGCTGAGCAGCTAGGTTTCCTTGGGTAAGGAAGTGAGGAGGTATATATTTTG
GAACAAGATAGGTCCAGAGTCAAACCTCACTTAGGGGTTACTGGGATACTGGGC
mm4_intergenic_5830416P10Rik|4833407H14Rik_chr19_53456855 GGCCACGTGACTCTATTCCAACAACCAGAGACCATGTGACTCTATTCCAACAACAATCAGAGACAGTGTGACTCTATTCC
AAGTACTGAGCTACTAAGGAGCACTGGGGAACTAAGGCCTGGGCCCTTCTGCTGCTAC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.