CRISPOR

DNA break repair outcome predictions

Guide: GCTCCCCACTGCCATCTCCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTG`	(Wild-type sequence)
382	`GGTGACCCTAGCTCCCCACTGCCATCTCCG---------TCGACACGGCGCTGGGGCTAC`	`9 bp deleted → no frameshift`
362	`GGTGACCCTAGCTCCCCACTGCCATCTCCG--GTCGCCGTCGACACGGCGCTGGGGCTAC`	`2 bp deleted → frameshift`
296	`GGTGACCCTAGCTCCCCACTGCCATCTCCG------CCGTCGACACGGCGCTGGGGCTAC`	`6 bp deleted → no frameshift`
283	`GGTGACCCTAGCTCCCCACTGCC---------------GTCGACACGGCGCTGGGGCTAC`	`15 bp deleted → no frameshift`
219	`GGTGACCCTAGCTCCCCACTGCCATCTCCG------------ACACGGCGCTGGGGCTAC`	`12 bp deleted → no frameshift`
211	`GGTGACCCTAGCTCCCCACTGCCATCTC-------GCCGTCGACACGGCGCTGGGGCTAC`	`7 bp deleted → frameshift`
191	`GGTGACCCTAGCTCCCCACTGCCATC---------GCCGTCGACACGGCGCTGGGGCTAC`	`9 bp deleted → no frameshift`
170	`GGTGACCCTAGCTCCCCACTGCCATCTCCG-----------------GCGCTGGGGCTAC`	`17 bp deleted → frameshift`
156	`GGTGACCCTAGCTCCCCACTGCCATCTC-------------GACACGGCGCTGGGGCTAC`	`13 bp deleted → frameshift`
147	`GGTGACCCTAGCTCCCCACTGCCATCTCCG--------------------CTGGGGCTAC`	`20 bp deleted → frameshift`
141	`GGTGACCCTAGCTCCCCACTGCCATC---------------GACACGGCGCTGGGGCTAC`	`15 bp deleted → no frameshift`
140	`GGTGACCCTAGCTCCCC---------------------GTCGACACGGCGCTGGGGCTAC`	`21 bp deleted → no frameshift`
133	`GGTGACCCTAGCTCCC----------------------GTCGACACGGCGCTGGGGCTAC`	`22 bp deleted → frameshift`
129	`GGTGACCCTAGCTCCCCAC---------------------------GGCGCTGGGGCTAC`	`27 bp deleted → no frameshift`
126	`GGTGACCCTAGCTCC-----------------------GTCGACACGGCGCTGGGGCTAC`	`23 bp deleted → frameshift`
114	`GGTGACCCTAGC-------------------------CGTCGACACGGCGCTGGGGCTAC`	`25 bp deleted → frameshift`
106	`GGT-------------------------------CGCCGTCGACACGGCGCTGGGGCTAC`	`31 bp deleted → frameshift`
105	`GGTGACCCTAGCTCCCCACTGCCA---------------------CGGCGCTGGGGCTAC`	`21 bp deleted → no frameshift`
105	`GGTGACCCTAGCTC---------------------GCCGTCGACACGGCGCTGGGGCTAC`	`21 bp deleted → no frameshift`
103	`GGTGACCCTAGCTCCCCACTGC---------------------------GCTGGGGCTAC`	`27 bp deleted → no frameshift`
101	`GGTGACCCTAGCTCCCCACTG--------------------------------GGGCTAC`	`32 bp deleted → frameshift`
94	`GGTGACCCTAGCTCCCCACTGC-----------------------------TGGGGCTAC`	`29 bp deleted → frameshift`
89	`GGTGACCC------------------------------GTCGACACGGCGCTGGGGCTAC`	`30 bp deleted → no frameshift`
86	`GGTGACCCTAGCTCCCCACTGCCATCT-------------------------GGGGCTAC`	`25 bp deleted → frameshift`
86	`GGTGACCCTAGCTCCCCAC-------------------------ACGGCGCTGGGGCTAC`	`25 bp deleted → frameshift`
84	`GGTGACC-------------------------------GTCGACACGGCGCTGGGGCTAC`	`31 bp deleted → frameshift`
77	`GGTGACCCTAGCTC---------------------------GACACGGCGCTGGGGCTAC`	`27 bp deleted → no frameshift`
75	`GGTGAC--------------------------------------ACGGCGCTGGGGCTAC`	`38 bp deleted → frameshift`
71	`GGTGACCCTAGCT---------------------------------------GGGGCTAC`	`39 bp deleted → no frameshift`
69	`GGTGACCCTAGCTCCCCACTGC-----------------------------------TAC`	`35 bp deleted → frameshift`
63	`GGTGACCCTAGCTCCCCACTGCCATCT-------------------------------AC`	`31 bp deleted → frameshift`
62	`GGTGACCCTAGC-------------------------------------GCTGGGGCTAC`	`37 bp deleted → frameshift`
52	`GGTGACCCTAGCT---------------------------------------------AC`	`45 bp deleted → no frameshift`
47	`GGT-------------------------------------CGACACGGCGCTGGGGCTAC`	`37 bp deleted → frameshift`
45	`GGTGACCCTAGCTCCCCACT--------------------------------------AC`	`38 bp deleted → frameshift`
40	`GGTGAC----------------------------------------GGCGCTGGGGCTAC`	`40 bp deleted → frameshift`
40	`GG----------------------------------------------CGCTGGGGCTAC`	`46 bp deleted → frameshift`
38	`GGTGACCCTAGCTCCCCAC-----------------------------------------`	`41 bp deleted → frameshift`
34	`GGTGACCCT-------------------------------------------GGGGCTAC`	`43 bp deleted → frameshift`
34	`GGTGACCCTA-------------------------------------------------C`	`49 bp deleted → frameshift`
29	`GG----------------------------------------------------GGCTAC`	`52 bp deleted → frameshift`
28	`GG-----------------------------------------------------GCTAC`	`53 bp deleted → frameshift`
26	`GG------------------------------------------------------CTAC`	`54 bp deleted → no frameshift`
25	`GGTG-------------------------------------------------GGGCTAC`	`49 bp deleted → frameshift`
20	`GGTGAC------------------------------------------------------`	`54 bp deleted → no frameshift`

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