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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313732_F | TCGTCGGCAGCGTCCGCATCACAAGGTGACCCTA | 59.7 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313732_R | GTCTCGTGGGCTCGGCTCCTAATCCCAGGCTCCCT | 60.1 | 1.00 |
| mm3_intron_Ccdc126_chr6_49332932_F | TCGTCGGCAGCGTCGGGTTGCCCATGTGTGCT | 60.6 | 0.92 |
| mm3_intron_Ccdc126_chr6_49332932_R | GTCTCGTGGGCTCGGACATGCCGAATTTAATCCACCT | 58.3 | 0.92 |
| mm4_intergenic_Flt1|Pomp_chr5_147777810_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Flt1|Pomp_chr5_147777810_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_exon_Gpi1_chr7_34208862_F | TCGTCGGCAGCGTCAGACCAAGCCCTCAAACCTG | 59.8 | 0.36 |
| mm4_exon_Gpi1_chr7_34208862_R | GTCTCGTGGGCTCGGCACGGCACAGGTATGAGGTA | 59.1 | 0.36 |
| mm3_intron_Gm21972/Armc9_chr1_86156606_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm3_intron_Gm21972/Armc9_chr1_86156606_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Amer2|Spata13_chr14_60416224_F | TCGTCGGCAGCGTCAGACACATCATGGGCTTCCC | 59.7 | 0.31 |
| mm4_intergenic_Amer2|Spata13_chr14_60416224_R | GTCTCGTGGGCTCGGGCCTGATATGTCCTGGGCTC | 59.9 | 0.31 |
| mm4_intergenic_Dgkg|Crygs_chr16_22661539_F | TCGTCGGCAGCGTCTCACCTTGGTCACACAGCTC | 59.8 | 0.30 |
| mm4_intergenic_Dgkg|Crygs_chr16_22661539_R | GTCTCGTGGGCTCGGAGCCTTGCCTTCACCAAAGT | 60.1 | 0.30 |
| mm4_intron_Tnxb_chr17_34702158_F | TCGTCGGCAGCGTCGGGAATGTCTACCAGGCCAC | 60.1 | 0.30 |
| mm4_intron_Tnxb_chr17_34702158_R | GTCTCGTGGGCTCGGCTGGCCTCACAGTCAGGATC | 59.8 | 0.30 |
| mm4_intron_Tnxa_chr17_34780881_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Tnxa_chr17_34780881_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm19965|Gm24030_chr1_117144638_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm19965|Gm24030_chr1_117144638_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_exon_D130020L05Rik/Smek1_chr12_101083531_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_exon_D130020L05Rik/Smek1_chr12_101083531_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Npl|Rgs8_chr1_153572394_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Npl|Rgs8_chr1_153572394_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3_intergenic_Cbx8|Cbx4/Gm26508_chr11_119053464_F | TCGTCGGCAGCGTCGGGAGCGGAGAACAAGGTAT | 59.1 | 0.25 |
| mm3_intergenic_Cbx8|Cbx4/Gm26508_chr11_119053464_R | GTCTCGTGGGCTCGGAATGAGCGTGCACCAGCT | 59.9 | 0.25 |
| mm4_intron_Mylk_chr16_34853441_F | TCGTCGGCAGCGTCGAGCCACCACAGAACACTGA | 59.8 | 0.23 |
| mm4_intron_Mylk_chr16_34853441_R | GTCTCGTGGGCTCGGGGGTTCCAATGGTGGTGTCT | 59.8 | 0.23 |
| mm4_intergenic_Slc38a4|Amigo2_chr15_97150789_F | TCGTCGGCAGCGTCTTGGGGATGAGCATGTGTGT | 59.5 | 0.22 |
| mm4_intergenic_Slc38a4|Amigo2_chr15_97150789_R | GTCTCGTGGGCTCGGTTAAAGAGCTTCTAGGCAACAAAA | 57.0 | 0.22 |
| mm4_intergenic_A1bg|Gm24696_chr15_61211064_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_A1bg|Gm24696_chr15_61211064_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_1700112E06Rik|Gm5670_chr14_22487043_F | TCGTCGGCAGCGTCCTGGTATTGTCTGTTAGGCCCA | 59.7 | 0.17 |
| mm4_intergenic_1700112E06Rik|Gm5670_chr14_22487043_R | GTCTCGTGGGCTCGGACAGTGGAAGGCAAGTCCTA | 58.2 | 0.17 |
| mm4_intron_Tc2n_chr12_101694122_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Tc2n_chr12_101694122_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Iffo1_chr6_125147368_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Iffo1_chr6_125147368_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Zbbx_chr3_75069320_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Zbbx_chr3_75069320_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23452|Gm23900_chr2_81303419_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23452|Gm23900_chr2_81303419_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Arsb|Gm25534_chr13_93893458_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Arsb|Gm25534_chr13_93893458_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Lrrc37a_chr11_103477118_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Lrrc37a_chr11_103477118_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Gm26815_chr8_121344603_F | TCGTCGGCAGCGTCCCCCTTCTGTGCCTTCAGAC | 60.3 | 0.12 |
| mm4_intron_Gm26815_chr8_121344603_R | GTCTCGTGGGCTCGGATCCACCAGGTGCACAGATG | 60.0 | 0.12 |
| mm4_intron_Adra1b_chr11_43836605_F | TCGTCGGCAGCGTCAATGCGGGTCCAGCCAAG | 60.3 | 0.11 |
| mm4_intron_Adra1b_chr11_43836605_R | GTCTCGTGGGCTCGGGATGCCCACACCCGGATC | 60.2 | 0.11 |
| mm4_intergenic_E130006D01Rik|Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1_chr5_111874177_F | TCGTCGGCAGCGTCGTAACTCCCTGGCGTCTGAG | 59.8 | 0.11 |
| mm4_intergenic_E130006D01Rik|Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1_chr5_111874177_R | GTCTCGTGGGCTCGGGGGAAAGGAGGTTCTGAGCC | 60.0 | 0.11 |
| mm4_intergenic_Gm1815|Zfp353-ps_chr8_41761984_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm1815|Zfp353-ps_chr8_41761984_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Rpl19|Stac2_chr11_98036106_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Rpl19|Stac2_chr11_98036106_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Cachd1_chr4_100944831_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Cachd1_chr4_100944831_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Slc7a7|Mrpl52_chr14_54414180_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Slc7a7|Mrpl52_chr14_54414180_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm6421|Hcn1_chr13_117436444_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm6421|Hcn1_chr13_117436444_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Gm26228|Gdf10_chr14_33683839_F | TCGTCGGCAGCGTCAAATGCTGTGCTCGGCTGTA | 60.3 | 0.09 |
| mm3_intergenic_Gm26228|Gdf10_chr14_33683839_R | GTCTCGTGGGCTCGGTCTCAGCACTAGCCATCCCT | 60.0 | 0.09 |
| mm3_intron_Polrmt_chr10_79744183_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intron_Polrmt_chr10_79744183_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Col26a1_chr5_136805943_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Col26a1_chr5_136805943_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm11747|Gm11748/Engase_chr11_118473847_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm11747|Gm11748/Engase_chr11_118473847_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Slc14a2_chr18_78163491_F | TCGTCGGCAGCGTCAGCAGGCTTAGACCCATTTCT | 59.0 | 0.06 |
| mm4_intron_Slc14a2_chr18_78163491_R | GTCTCGTGGGCTCGGGCAGCCAGCTTTGGAACTTT | 59.6 | 0.06 |
| mm4_intergenic_Pih1d2|Dlat_chr9_50625650_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Pih1d2|Dlat_chr9_50625650_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm26825/Gm15459|Gm23838_chr5_5949438_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm26825/Gm15459|Gm23838_chr5_5949438_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7148890_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7148890_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56417670_F | TCGTCGGCAGCGTCGAGTGGGATGTGTCTCTCAGT | 58.8 | 0.05 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56417670_R | GTCTCGTGGGCTCGGAGCTACAGGGGCACATAGGA | 60.0 | 0.05 |
| mm3_intergenic_Bmpr1b|Pdlim5_chr3_142190487_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Bmpr1b|Pdlim5_chr3_142190487_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Mir680-2|Alg13_chrX_144302043_F | TCGTCGGCAGCGTCTCTATAACTTGCCGCTCCGA | 58.6 | 0.04 |
| mm3_intergenic_Mir680-2|Alg13_chrX_144302043_R | GTCTCGTGGGCTCGGTTGTGAGATGGCCATGGGTC | 60.0 | 0.04 |
| mm4_exon_Cnga2_chrX_72005008_F | TCGTCGGCAGCGTCCCTGAGCTCCAGACTGTGAC | 59.7 | 0.04 |
| mm4_exon_Cnga2_chrX_72005008_R | GTCTCGTGGGCTCGGAGGTGTGGAAGCCTTTGGAC | 60.1 | 0.04 |
| mm4_exon_Rhof_chr5_123132419_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Rhof_chr5_123132419_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm23296|Akap6_chr12_52640335_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm23296|Akap6_chr12_52640335_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Lgalsl_chr11_20829985_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Lgalsl_chr11_20829985_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cdh23_chr10_60307587_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cdh23_chr10_60307587_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24861|Chodl_chr16_78845969_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24861|Chodl_chr16_78845969_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Nubpl_chr12_52273480_F | TCGTCGGCAGCGTCTTGCATGCCCAATTTCTGCA | 59.3 | 0.03 |
| mm4_intron_Nubpl_chr12_52273480_R | GTCTCGTGGGCTCGGTGCTACAGGAATCCCAAAGAGG | 59.7 | 0.03 |
| mm2_intergenic_Gm22338|Tank_chr2_61566532_F | TCGTCGGCAGCGTCACCTGCTATTTACTGTGGCCA | 59.3 | 0.03 |
| mm2_intergenic_Gm22338|Tank_chr2_61566532_R | GTCTCGTGGGCTCGGCCTGTACCTTTTCTGGGATAGGC | 60.4 | 0.03 |
| mm4_intron_Zfp521_chr18_13708656_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zfp521_chr18_13708656_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arhgap24_chr5_102576145_F | TCGTCGGCAGCGTCGGCTGTGTTCACTGAGGAGA | 59.3 | 0.03 |
| mm4_intron_Arhgap24_chr5_102576145_R | GTCTCGTGGGCTCGGACAGTTCAATGCCAACAGCT | 58.3 | 0.03 |
| mm4_intergenic_Gm6133|Gm26430_chr18_78515707_F | TCGTCGGCAGCGTCTCCCCTACTTCTGTGGTCCT | 59.2 | 0.02 |
| mm4_intergenic_Gm6133|Gm26430_chr18_78515707_R | GTCTCGTGGGCTCGGTGCCCATTCTTGCAGCCTTA | 59.9 | 0.02 |
| mm4_intergenic_Gm26969|Dleu7_chr14_62204564_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26969|Dleu7_chr14_62204564_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Bbx_chr16_50403185_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Bbx_chr16_50403185_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Exo1|Pld5_chr1_175959381_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Exo1|Pld5_chr1_175959381_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zcwpw2_chr9_117906984_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zcwpw2_chr9_117906984_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ttc29|Pou4f2_chr8_78420011_F | TCGTCGGCAGCGTCTCAAAGGGGACAGGGATTGC | 59.9 | 0.00 |
| mm4_intergenic_Ttc29|Pou4f2_chr8_78420011_R | GTCTCGTGGGCTCGGCTTCTCCACCATCACCACCC | 60.0 | 0.00 |
| mm4_intergenic_Smchd1|Ndc80_chr17_71493094_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Smchd1|Ndc80_chr17_71493094_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pja1|Gm14816_chrX_99479631_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pja1|Gm14816_chrX_99479631_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5839|Gm25440_chr18_49280976_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5839|Gm25440_chr18_49280976_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4889|Dusp4_chr8_34578794_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4889|Dusp4_chr8_34578794_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13837|Ccdc60_chr5_116268821_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13837|Ccdc60_chr5_116268821_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313732 |
CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTG CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAG |
| mm3_intron_Ccdc126_chr6_49332932 |
GGGTTGCCCATGTGTGCTCCTGCGCCTGCCTTGTTACCTTTACATTACTGTATTATATTCATTTAGTTGTAGTCTCAAAG TAGGCTTTATTTGGTTTTGTTATTTCCTAGTAGGTGGATTAAATTCGGCATGT |
| mm4_exon_Gpi1_chr7_34208862 |
AGACCAAGCCCTCAAACCTGTGCATGACACATACCTCAGACCCAACTTAAGAGCTTCATACCTGTGCCTACCTTATCGCC TCAGACACCGATGGTAAATTTCATACCTCATACCTGTGCCGTG |
| mm4_intergenic_Amer2|Spata13_chr14_60416224 |
AGACACATCATGGGCTTCCCGTTTGCATGCTCCAGTGAAAGGTGAGAAGGCAGGAGCTGGAGGAGCCCAGGACATATCAG GC |
| mm4_intergenic_Dgkg|Crygs_chr16_22661539 |
TCACCTTGGTCACACAGCTCGCTTTGAACTTCACAATCAACAGGATCTTTCTGTTTTCTCAAAAGACGACAGGGCAGGAG CTGGCCTCATCTAGTCCTAACCTTGTTTAACCTTGACTTTGGTGAAGGCAAGGCT |
| mm4_intron_Tnxb_chr17_34702158 |
GGGAATGTCTACCAGGCCACACCTCTTAAGGGACCCTGCTTCTGCCCTGTCACCTTAGAGACCAGTCATTTAATGAGTAG CATCTGAGGGACAGATGATCCTGACTGTGAGGCCAG |
| mm3_intergenic_Cbx8|Cbx4/Gm26508_chr11_119053464 |
GGGAGCGGAGAACAAGGTATAGCCTGTATTTAGGTTCCAGTCCTTATATGGCGATGCGCACGAGCGCCAGCCGAGCGGCC GCACCATATAAGGACACACGGCAGGCGCCGGGCTGAGCTGGTGCACGCTCATT |
| mm4_intron_Mylk_chr16_34853441 | GAGCCACCACAGAACACTGACATCCCGGGCCTGCCTTGTCAGCTAAAGACACCACCATTGGAACCC |
| mm4_intergenic_Slc38a4|Amigo2_chr15_97150789 |
TTGGGGATGAGCATGTGTGTAGGAATGAAGTCAAAGAAGAAAGGGAACAAGGCAGGCCCTGGTTGATTTATAGGTAACAA CGATTTTTGTTGCCTAGAAGCTCTTTAA |
| mm4_intergenic_1700112E06Rik|Gm5670_chr14_22487043 |
CTGGTATTGTCTGTTAGGCCCATCAGGCAACAAGGCAGGCCAAGGTAAAACAGATGAGCATAAGTTGACAACAGATTAAA AATAGTGGAGTAAAGTCCATTAATAATTCTCATATTTAGGACTTGCCTTCCACTGT |
| mm4_intron_Gm26815_chr8_121344603 |
CCCCTTCTGTGCCTTCAGACATTTTCGGAGGACTTCATGAGTCCTCTGCAAGTCCACAAGGCAGGCACAGGAGATGGGAG ATGGGAATCACAGGGTAATCGCTTTGCCAAGCGACCATCTGTGCACCTGGTGGAT |
| mm4_intron_Adra1b_chr11_43836605 |
AATGCGGGTCCAGCCAAGGAGCGCAGCCCCGACGCCAGCGCCAGCCTAGTCGGCTTGGAGACCTGCCGCGCCCGGCACCG CTCCCTCTGCGGGGATGGGGCGGGGAGCAGAGCAGGGGATCCGGGTGTGGGCATC |
| mm4_intergenic_E130006D01Rik|Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1_chr5_111874177 |
GTAACTCCCTGGCGTCTGAGGGGTGAGGCTTCCCGCCCCTGCTTTGTCTCCTTTGGCCACACATGCAGCGTCCACTTGAG CTTCCATTGGGGGTGGAGGCTCAGAACCTCCTTTCCC |
| mm3_intergenic_Gm26228|Gdf10_chr14_33683839 |
AAATGCTGTGCTCGGCTGTAGTAAGGCGTCACCGCAGGCGCAGGCCGTCATCTGTCTTCATCAAGCCAGCCTGAGCAGAG TGACAGAGCCATCCAGAGGGAGCAGGGATGGCTAGTGCTGAGA |
| mm4_intron_Slc14a2_chr18_78163491 |
AGCAGGCTTAGACCCATTTCTCCACAGTCAAGGGGACAAGGCAGCCTCTGGGCTGACTGGCCAGTCACTGTGACAAAGTT CCAAAGCTGGCTGC |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56417670 |
GAGTGGGATGTGTCTCTCAGTACTGTTTCTCCCTGGGCCTGCGGTCTCGCCTTATATGTATTTTCCTATGTGCCCCTGTA GCT |
| mm3_intergenic_Mir680-2|Alg13_chrX_144302043 |
TCTATAACTTGCCGCTCCGATATCAGCGCCTGCCTTGTCTGCTTCCTTTTCTCTGTCCAAGGAAAACAATCACGTATTCT ATGTTCACATGCTGACCACCTACCAGTACTGTGACCCATGGCCATCTCACAA |
| mm4_exon_Cnga2_chrX_72005008 |
CCTGAGCTCCAGACTGTGACAACCCATCAGGGGGATGGCAAAGGCGACAAGGACGGCGAGGGAAAGGGCACCAAGTATAT CTAGCTAGCCTTCGGGGGTTGGGGGGGTGTCCAAAGGCTTCCACACCT |
| mm4_intron_Nubpl_chr12_52273480 |
TTGCATGCCCAATTTCTGCATGACCTTTAAAAGAAAGCCTTCTGCTCTAATGCCCAGCCCCTGCCTTGTCCCCAGACCTC CCAGATAGGACTCATTTATCCCTCTTTGGGATTCCTGTAGCA |
| mm2_intergenic_Gm22338|Tank_chr2_61566532 |
ACCTGCTATTTACTGTGGCCAAGTTCACTTGTCTCTGAGCCTGCCTTGTCGCCTGAAAAATATCAAATTTGCAAGATAAT TAAGAAAATAAAATGAAAATAGCCTATCCCAGAAAAGGTACAGG |
| mm4_intron_Arhgap24_chr5_102576145 |
GGCTGTGTTCACTGAGGAGATGGTATTTGTATAGAGATGTTAGCAAATGCTGTAAATCTGAGGAGACATGGCAGGGGCAG GAAGCTGTTGGCATTGAACTGT |
| mm4_intergenic_Gm6133|Gm26430_chr18_78515707 |
TCCCCTACTTCTGTGGTCCTTCAGATAGTTTCCATAGCCAACACGATTAATGGTGCAGAAATCAGTTATCAAGGCGAGAA GGCAGGCTGTGGGTAGATAAGGCTGCAAGAATGGGCA |
| mm4_intergenic_Ttc29|Pou4f2_chr8_78420011 |
TCAAAGGGGACAGGGATTGCTTTGTTAAGGCGAGAGGGGAGGCGATGGTGTGGAGGGTTTCCTGTGATTTCTGGTAACTT GTGGGAACTGTTAAATTCCGCGGCGGGGGTCGGGGGTGGGGGTGGTGATGGTGGAGAAG |