CRISPOR

DNA break repair outcome predictions

Guide: GAGCCGGCCCTCCGACTTCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCG`	(Wild-type sequence)
269	`CTGAGCCCGCGAGCCGG----------------GAGCGTGGCGGGGGAGGCCAGGAGGAG`	`16 bp deleted → frameshift`
268	`CTGAGCCCGCGAGCCGGCCCTCCG--------GGAGCGTGGCGGGGGAGGCCAGGAGGAG`	`8 bp deleted → frameshift`
221	`CTGAGCCCGCGAGC-----------------------GTGGCGGGGGAGGCCAGGAGGAG`	`23 bp deleted → frameshift`
198	`CTGAGCCCGCGAGCCGGCCCTCCG--------------TGGCGGGGGAGGCCAGGAGGAG`	`14 bp deleted → frameshift`
182	`CTGAGCCCGCGAGCCGGCCCTCCGA----------GCGTGGCGGGGGAGGCCAGGAGGAG`	`10 bp deleted → frameshift`
180	`CTGAGCCCGCGAGCCGGC------------------------GGGGGAGGCCAGGAGGAG`	`24 bp deleted → no frameshift`
170	`CTGAGCCCGCGAGCCGG-----------------AGCGTGGCGGGGGAGGCCAGGAGGAG`	`17 bp deleted → frameshift`
155	`CTGAGCCCGCGAGCCGG---------------------------GGGAGGCCAGGAGGAG`	`27 bp deleted → no frameshift`
155	`CTGAGCCCGCG---------------------------TGGCGGGGGAGGCCAGGAGGAG`	`27 bp deleted → no frameshift`
154	`CTGAGCCCGCGAGCCGGCCCTCCG-------------------GGGGAGGCCAGGAGGAG`	`19 bp deleted → frameshift`
154	`CTGAGCCCGCGAGCCGGC-------------------GTGGCGGGGGAGGCCAGGAGGAG`	`19 bp deleted → frameshift`
153	`CTGAGCCCGCGAGCCGGCC---------------------------------AGGAGGAG`	`33 bp deleted → no frameshift`
148	`CTGAGC-------------------------------GTGGCGGGGGAGGCCAGGAGGAG`	`31 bp deleted → frameshift`
140	`CTGAGCCCGCG---------------------GGAGCGTGGCGGGGGAGGCCAGGAGGAG`	`21 bp deleted → no frameshift`
133	`CTGAGCCCGCGAGCCGGCCCTCCGACTTCC----------------------AGGAGGAG`	`22 bp deleted → frameshift`
133	`CTGAGCCCGCGAGCCG----------------------TGGCGGGGGAGGCCAGGAGGAG`	`22 bp deleted → frameshift`
126	`CTGAGCCCG-----------------------GGAGCGTGGCGGGGGAGGCCAGGAGGAG`	`23 bp deleted → frameshift`
121	`CTGAGCCCGCG--------------------------------GGGGAGGCCAGGAGGAG`	`32 bp deleted → frameshift`
98	`CTGAGCCCGCGAGCCGG----------------------------GGAGGCCAGGAGGAG`	`28 bp deleted → frameshift`
98	`CTGAGCCCGCGAGC----------------------------GGGGGAGGCCAGGAGGAG`	`28 bp deleted → frameshift`
95	`CTGAGCCCGCGAGCCGGCCCTCCGA-----------------------GGCCAGGAGGAG`	`23 bp deleted → frameshift`
94	`CTGAGCCCGCGAGCC-------------------------------------AGGAGGAG`	`37 bp deleted → frameshift`
94	`CTGAGCCCGCGAGCCGGCCCTCC-----------------------------AGGAGGAG`	`29 bp deleted → frameshift`
94	`CTGAGCCCGCGAGCCGG-----------------------------GAGGCCAGGAGGAG`	`29 bp deleted → frameshift`
94	`CTGAGCCCG-----------------------------TGGCGGGGGAGGCCAGGAGGAG`	`29 bp deleted → frameshift`
89	`CTGAGCCCGCGAGCCGG------------------------------AGGCCAGGAGGAG`	`30 bp deleted → no frameshift`
82	`CTGAGCCCGCGAG------------------------------------GCCAGGAGGAG`	`36 bp deleted → no frameshift`
80	`CTGAGCCCGCGAGCCGGCCC--------------------------------AGGAGGAG`	`32 bp deleted → frameshift`
73	`CTGAGCCCG----------------------------------GGGGAGGCCAGGAGGAG`	`34 bp deleted → frameshift`
66	`CTGAGC------------------------------------GGGGGAGGCCAGGAGGAG`	`36 bp deleted → no frameshift`
63	`CTGAGCCCGCGAGCCGGCCCTCCGA-------------------------------GGAG`	`31 bp deleted → frameshift`
63	`CTGAGCC---------------------------------------------AGGAGGAG`	`45 bp deleted → no frameshift`
60	`CTGAGCCCGCGAGCCGG--------------------------------------AGGAG`	`38 bp deleted → frameshift`
55	`CTGAGCCCGCGAG--------------------------------------------GAG`	`44 bp deleted → frameshift`
55	`CTGAG--------------------------------------------GCCAGGAGGAG`	`44 bp deleted → frameshift`
54	`CTGAGCCCGCGAGCCGGCCCTCCGA----------------------------------G`	`34 bp deleted → frameshift`
51	`CTGAGCCCGCGAGCCGG-----------------------------------------AG`	`41 bp deleted → frameshift`
51	`CTGAGCCCGC-----------------------------------------CAGGAGGAG`	`41 bp deleted → frameshift`
47	`CTGAGCCCGCGAG-----------------------------------------------`	`47 bp deleted → frameshift`
47	`CTG-------------------------------------GCGGGGGAGGCCAGGAGGAG`	`37 bp deleted → frameshift`
44	`CTGAGCCC--------------------------------------------AGGAGGAG`	`44 bp deleted → frameshift`
38	`CTGAGCCCGCGAG-----------------------------------------GAGGAG`	`41 bp deleted → frameshift`
37	`CTGAG----------------------------------------------------GAG`	`52 bp deleted → frameshift`
32	`CTGAG-------------------------------------------------------`	`55 bp deleted → frameshift`
25	`CTGAG-------------------------------------------------GAGGAG`	`49 bp deleted → frameshift`

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