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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314006_F | TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314006_R | GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC | 60.2 | 1.00 |
| mm3_intergenic_Slc8a1|Rpl31-ps25_chr17_81672239_F | TCGTCGGCAGCGTCCCCTCTGCTACAAACTGCCA | 59.9 | 0.81 |
| mm3_intergenic_Slc8a1|Rpl31-ps25_chr17_81672239_R | GTCTCGTGGGCTCGGACAAGCTTCTCTCTGTGCCC | 59.9 | 0.81 |
| mm4_intron_Neurl1a_chr19_47235630_F | Primer3: not found at this Tm | N.d. | 0.59 |
| mm4_intron_Neurl1a_chr19_47235630_R | Primer3: not found at this Tm | N.d. | 0.59 |
| mm4_intergenic_Slc4a1|Bloodlinc_chr11_102373808_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Slc4a1|Bloodlinc_chr11_102373808_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Sec14l4_chr11_4044317_F | TCGTCGGCAGCGTCCTTGGACCAGTCTGGGTTCC | 59.9 | 0.45 |
| mm4_intron_Sec14l4_chr11_4044317_R | GTCTCGTGGGCTCGGACCCTGAAAACTGCAACCCT | 59.7 | 0.45 |
| mm4_intergenic_Spp2|Glrp1_chr1_88451522_F | TCGTCGGCAGCGTCAGCATCACAGCTTGGGACAG | 60.3 | 0.42 |
| mm4_intergenic_Spp2|Glrp1_chr1_88451522_R | GTCTCGTGGGCTCGGCAGAAGGGCCACCAGAAGAG | 60.0 | 0.42 |
| mm4_exon_Gm12827/Rnf220_chr4_117496953_F | TCGTCGGCAGCGTCGCGCTGGCTCTAATCAGTGA | 60.1 | 0.38 |
| mm4_exon_Gm12827/Rnf220_chr4_117496953_R | GTCTCGTGGGCTCGGTGCCTGTATGTGTGTGAGTGT | 59.5 | 0.38 |
| mm4_intron_Asap1_chr15_64110042_F | TCGTCGGCAGCGTCTGACAGTGTTGCGGAAGACA | 59.8 | 0.34 |
| mm4_intron_Asap1_chr15_64110042_R | GTCTCGTGGGCTCGGGCCCTTCACCTCCAAGTCTC | 60.0 | 0.34 |
| mm4_intron_5730446D14Rik_chr6_52165997_F | TCGTCGGCAGCGTCCTCGGGTGCGGTGAGTATTT | 60.1 | 0.34 |
| mm4_intron_5730446D14Rik_chr6_52165997_R | GTCTCGTGGGCTCGGCAGTCCCCAACCAGAGTGAC | 59.9 | 0.34 |
| mm4_intron_Elmo1_chr13_20091378_F | TCGTCGGCAGCGTCTAGAGAGAGGCTAGCGTGGG | 60.1 | 0.24 |
| mm4_intron_Elmo1_chr13_20091378_R | GTCTCGTGGGCTCGGTTCCTCTTCCTCTAAGCCGC | 59.1 | 0.24 |
| mm4_intergenic_Arhgap6|Amelx_chrX_169137464_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Arhgap6|Amelx_chrX_169137464_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Eif5a_chr11_69919624_F | TCGTCGGCAGCGTCGCTAGGGAAAGGAAGCCTCC | 59.8 | 0.21 |
| mm4_intron_Eif5a_chr11_69919624_R | GTCTCGTGGGCTCGGCAGTGGCGAGTAACACCTGA | 59.6 | 0.21 |
| mm4_intergenic_Gm13552|Tanc1_chr2_59585063_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm13552|Tanc1_chr2_59585063_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Scn1a_chr2_66279705_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Scn1a_chr2_66279705_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_exon_Rhpn2_chr7_35381548_F | TCGTCGGCAGCGTCCTTCGTGAGTGCTGGGAACT | 59.9 | 0.17 |
| mm4_exon_Rhpn2_chr7_35381548_R | GTCTCGTGGGCTCGGCGTGAACCCTGCCAGATACC | 60.4 | 0.17 |
| mm4_intron_Gm9994_chr1_93934072_F | TCGTCGGCAGCGTCTCTACAGAGGGACCAAGGCA | 59.8 | 0.17 |
| mm4_intron_Gm9994_chr1_93934072_R | GTCTCGTGGGCTCGGACCCTAGCTAGGATGGGACA | 59.0 | 0.17 |
| mm4_intron_Gm6086_chr1_94002349_F | TCGTCGGCAGCGTCGGACCAAGGCATCTCCTCTG | 59.8 | 0.17 |
| mm4_intron_Gm6086_chr1_94002349_R | GTCTCGTGGGCTCGGACCCTAGCTAGGATGGGACA | 59.0 | 0.17 |
| mm4_intron_Gal3st2_chr1_93867904_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gal3st2_chr1_93867904_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Dag1_chr9_108222095_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Dag1_chr9_108222095_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Pcolce_chr5_137610689_F | TCGTCGGCAGCGTCCAGTCCAAGGACCACGGAAA | 59.8 | 0.15 |
| mm4_exon_Pcolce_chr5_137610689_R | GTCTCGTGGGCTCGGGGGGTAGAGGAGGTTAGGGG | 60.1 | 0.15 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50389420_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50389420_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Smad7|Ctif_chr18_75399344_F | TCGTCGGCAGCGTCGGGGCTGCTTGGATGAATCT | 60.1 | 0.14 |
| mm4_intergenic_Smad7|Ctif_chr18_75399344_R | GTCTCGTGGGCTCGGAGAAGATGCCCACTGTTTGG | 58.0 | 0.14 |
| mm4_intron_Tmem132d_chr5_128375399_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Tmem132d_chr5_128375399_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Dnah9_chr11_65909840_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Dnah9_chr11_65909840_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_AW554918_chr18_25455141_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_AW554918_chr18_25455141_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Tmem233|C330018A13Rik_chr5_116097250_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Tmem233|C330018A13Rik_chr5_116097250_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26930723_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26930723_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Eif1a|Cdo1_chr18_46661252_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Eif1a|Cdo1_chr18_46661252_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Foxn3_chr12_99438014_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Foxn3_chr12_99438014_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Nubp2_chr17_24883272_F | TCGTCGGCAGCGTCACTCTCTGGGCTATGGACGT | 60.0 | 0.12 |
| mm4_exon_Nubp2_chr17_24883272_R | GTCTCGTGGGCTCGGGCCTTTGTCATTGCAGGCTC | 60.1 | 0.12 |
| mm4_exon_Ttc16/Tor2a_chr2_32757438_F | TCGTCGGCAGCGTCCTGGGACGTGGCTTCTCTG | 60.0 | 0.11 |
| mm4_exon_Ttc16/Tor2a_chr2_32757438_R | GTCTCGTGGGCTCGGAGATGTTTCGAGCCCCTGC | 60.0 | 0.11 |
| mm4_intron_Gas7_chr11_67672255_F | TCGTCGGCAGCGTCCTCCTGGCTTCTGAAGGGTG | 60.0 | 0.11 |
| mm4_intron_Gas7_chr11_67672255_R | GTCTCGTGGGCTCGGGAGGGTGAGCATGCTCTTGT | 60.0 | 0.11 |
| mm4_intergenic_Gm24850|Gm25387_chr11_27265749_F | TCGTCGGCAGCGTCTGACCCTGTTTTCAGCTCCTC | 59.9 | 0.10 |
| mm4_intergenic_Gm24850|Gm25387_chr11_27265749_R | GTCTCGTGGGCTCGGGCATAGCAACACCAGCTTCC | 59.5 | 0.10 |
| mm4_intron_Slc12a3_chr8_94349275_F | TCGTCGGCAGCGTCGGGTCTGCTGTTAACCTGCT | 59.9 | 0.10 |
| mm4_intron_Slc12a3_chr8_94349275_R | GTCTCGTGGGCTCGGAGACTCCTTCTCAGACTGCCT | 59.9 | 0.10 |
| mm4_intergenic_Prosc|Gpr124_chr8_27068532_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Prosc|Gpr124_chr8_27068532_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Top2a|Igfbp4_chr11_99039387_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Top2a|Igfbp4_chr11_99039387_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Tug1/TUG1_2/TUG1_1|Tug1_chr11_3648649_F | TCGTCGGCAGCGTCCTCCAACTGGGTCCTAGCAG | 59.4 | 0.08 |
| mm3_intergenic_Tug1/TUG1_2/TUG1_1|Tug1_chr11_3648649_R | GTCTCGTGGGCTCGGGGTCGTCCGGGTGGTTGG | 62.6 | 0.08 |
| mm4_exon_Slc22a21_chr11_53979812_F | TCGTCGGCAGCGTCGAAGAAGATGAGGCGCTGGA | 59.8 | 0.07 |
| mm4_exon_Slc22a21_chr11_53979812_R | GTCTCGTGGGCTCGGGCTGCCGACTCTATGCTTGA | 60.1 | 0.07 |
| mm4_intron_Tspan9_chr6_127991121_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tspan9_chr6_127991121_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Gli2_chr1_118856978_F | TCGTCGGCAGCGTCTGTTCTTTTCCCCATCATCATCAC | 59.2 | 0.06 |
| mm4_intron_Gli2_chr1_118856978_R | GTCTCGTGGGCTCGGTGTGAGCTGAGGCAGTGAAG | 59.9 | 0.06 |
| mm4_exon_Ptprf_chr4_118231723_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Ptprf_chr4_118231723_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Rnaset2b_chr17_7001160_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Rnaset2b_chr17_7001160_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Polr1e_chr4_45019145_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Polr1e_chr4_45019145_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Ubtf_chr11_102318757_F | TCGTCGGCAGCGTCCAGCGGAAGAGTCACTCTGG | 60.1 | 0.05 |
| mm4_intron_Ubtf_chr11_102318757_R | GTCTCGTGGGCTCGGGGAAAGGGGTTGCTCTCTGG | 60.3 | 0.05 |
| mm4_intergenic_n-R5s172|Gm26486_chr5_53355431_F | TCGTCGGCAGCGTCCTCACCGGCCAGAGTGTATC | 59.8 | 0.04 |
| mm4_intergenic_n-R5s172|Gm26486_chr5_53355431_R | GTCTCGTGGGCTCGGCCCATGGATCCTTTGTGCCT | 60.0 | 0.04 |
| mm4_exon_Ptgs1_chr2_36230590_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ptgs1_chr2_36230590_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22745|Kcnj6_chr16_94898985_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22745|Kcnj6_chr16_94898985_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ext1_chr15_53345976_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Ext1_chr15_53345976_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Tbc1d2_chr4_46646037_F | TCGTCGGCAGCGTCCGTTCATTCCTGGTCCTGCT | 60.0 | 0.03 |
| mm4_intron_Tbc1d2_chr4_46646037_R | GTCTCGTGGGCTCGGCCACAGACCCAAGCATCTGA | 59.6 | 0.03 |
| mm4_intergenic_Nsmce2|Trib1_chr15_59636201_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nsmce2|Trib1_chr15_59636201_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Ubr5|Odf1_chr15_38178301_F | TCGTCGGCAGCGTCCCAGAAGCAGAAGTCCAGCA | 59.9 | 0.03 |
| mm3_intergenic_Ubr5|Odf1_chr15_38178301_R | GTCTCGTGGGCTCGGCCCCACCCCGAGAAAATCAT | 59.7 | 0.03 |
| mm4_intron_Kif5a_chr10_127256129_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Kif5a_chr10_127256129_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tgfbr3|Gm22557_chr5_107290813_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tgfbr3|Gm22557_chr5_107290813_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_6030419C18Rik_chr9_58489037_F | TCGTCGGCAGCGTCCGAGCGTGGTGAGGAGATTT | 60.1 | 0.02 |
| mm4_exon_6030419C18Rik_chr9_58489037_R | GTCTCGTGGGCTCGGCGGTGACCGGAGACATCTG | 59.8 | 0.02 |
| mm4_intron_Rbms1_chr2_60849976_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Rbms1_chr2_60849976_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ccdc6_chr10_70170226_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ccdc6_chr10_70170226_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Nudt4_chr10_95563813_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Nudt4_chr10_95563813_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Usp9x_chrX_13072697_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Usp9x_chrX_13072697_R | Primer3: not found at this Tm | N.d. | 0.01 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314006 |
AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA GGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG |
| mm3_intergenic_Slc8a1|Rpl31-ps25_chr17_81672239 |
CCCTCTGCTACAAACTGCCATAGAGCCCCGACTTCCAGGGAGAGTGAGGGGCAGCTATTTGGCTCAGCCTAGGAAAGGAG CTTTGAGAGAAGAGCGGCTGCTTTGAAAGGGCACAGAGAGAAGCTTGT |
| mm4_intron_Sec14l4_chr11_4044317 |
CTTGGACCAGTCTGGGTTCCCTCTATGTCCAACTTCCAGGGAGAGTGTAGGGACTGTAACTAAGGACTCCAGGGTTGCAG TTTTCAGGGT |
| mm4_intergenic_Spp2|Glrp1_chr1_88451522 |
AGCATCACAGCTTGGGACAGCCCTCCATGCTGCCAGCTGTTCTCTGCGGTCCTTGCTGCACACCACCATGCTCCTGGGAA GCCTGGCCAGCTCTTCTGGTGGCCCTTCTG |
| mm4_exon_Gm12827/Rnf220_chr4_117496953 |
GCGCTGGCTCTAATCAGTGAATGAGCCTCCACTCTCCCAGGGACTCGGAGGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTCACACTCACACACATACAGGCA |
| mm4_intron_Asap1_chr15_64110042 |
TGACAGTGTTGCGGAAGACAACAGAGAAGAGCCAGGGAGGCACACAAGTGAGCTTGGCCTTGCACGGAGAGGACAGACTG CCAGGGAGCGTGAGGGCCTTGGACGGTGCACGGGGAGACTTGGAGGTGAAGGGC |
| mm4_intron_5730446D14Rik_chr6_52165997 |
CTCGGGTGCGGTGAGTATTTCCGACTTCCAGGAAGAGTGCTGGAACTTGTCAGCAGGGAGGGAAAGGACTCTTGTTTCTT GGTCCCTCTTTCCCTCCTACTCTTCTTCGAGTCCTTGTCACTCTGGTTGGGGACTG |
| mm4_intron_Elmo1_chr13_20091378 |
TAGAGAGAGGCTAGCGTGGGAGGGGGCGTACCGCGCGTCTTCCCGGGAGAGCGGCGGGCATGGGGCGCCAGGGAGGGAGC GAGCGAGCTGCGCGCCGCGGTAGCCCCGCGGCTTAGAGGAAGAGGAA |
| mm4_intron_Eif5a_chr11_69919624 |
GCTAGGGAAAGGAAGCCTCCTGGGAGTTGGGATGCCAAATCCCAGAGATGAGGAAAGGGCTACTTCCCGGGAGCGAGTAG GACTCAGGTGTTACTCGCCACTG |
| mm4_exon_Rhpn2_chr7_35381548 |
CTTCGTGAGTGCTGGGAACTGTCTAGTGCCCGGGAGCGTGGGGGCCACACAGCCTGTTTTTATTTGTCTATCTGGTATCT GGCAGGGTTCACG |
| mm4_intron_Gm9994_chr1_93934072 |
TCTACAGAGGGACCAAGGCATCTCCTCTGGGGAGCTCCTGAGCTCTTTGTCTATACAGACTTTCAGGGAGTGTGGTGGGG ACACGACTGACACACATCAGAGGTGACATGTCCCATCCTAGCTAGGGT |
| mm4_intron_Gm6086_chr1_94002349 |
GGACCAAGGCATCTCCTCTGAGGAGCTCCTGAGCTCTTTGTCTATACAGACTTTCAGGGAGTGTGGTGGGGACATGACTG ACACACATCAGAGGTGACATGTCCCATCCTAGCTAGGGT |
| mm4_exon_Pcolce_chr5_137610689 |
CAGTCCAAGGACCACGGAAAGTGGGGCTAGGCAACCGCCCCGTCTCTCCATCCCCTAGAGGCTCCACCCCCACGCTGCCA GGAATTCCGGGAATCCTTCTCAGTCCCCCTAACCTCCTCTACCCC |
| mm4_intergenic_Smad7|Ctif_chr18_75399344 |
GGGGCTGCTTGGATGAATCTATGCTCAGTGACTGAGTTTCCGGGAGTGTGGGGGGATGCATTCATATTTCTACCAAATTT CCCTCCCAAACAGTGGGCATCTTCT |
| mm4_exon_Nubp2_chr17_24883272 |
ACTCTCTGGGCTATGGACGTGAGTGCGGAATATGCAGTGCTTTTGGGAAATTCCTGGATGAAGTCACGCCCTTCCTCCAA GCTCCTGGTAAGTTGGGAATCCAGGGGGACAGAGCCTGCAATGACAAAGGC |
| mm4_exon_Ttc16/Tor2a_chr2_32757438 |
CTGGGACGTGGCTTCTCTGCGCTGCACCTTCGGCTCGTTCTGCGAATGCGACTTCTGGCCCGACTTGCCGGGTGAGGGGT GGGAGCCGGTGCGGACGGGCGGGCAGCCGGCAGTTCGCAGGGGCTCGAAACATCT |
| mm4_intron_Gas7_chr11_67672255 |
CTCCTGGCTTCTGAAGGGTGTGAGTGCCAGAGACAGAGCTGGCATGGCCCCACCCCCACGCCCCCGGGGGGTGGGGAGTG ACATTTCCCAGCCCACAAGAGCATGCTCACCCTC |
| mm4_intergenic_Gm24850|Gm25387_chr11_27265749 |
TGACCCTGTTTTCAGCTCCTCTTCCCCCACCCTCCCTGGATGTCGCTGCTTGGTGCTGTTTCAGTACATTGAACACAAGA GCATATGCAGCATTTTGTTTGGTATAAATGTTTGGAAGCTGGTGTTGCTATGC |
| mm4_intron_Slc12a3_chr8_94349275 |
GGGTCTGCTGTTAACCTGCTATATAACTCAGCTCTGACTATGGCTGACTCTACCACAATTATGTCATGGGGTAGAAAATC TGGCCCCCAGGCTCCCGGGAACTCTATTGTCTAAGGCAGTCTGAGAAGGAGTCT |
| mm3_intergenic_Tug1/TUG1_2/TUG1_1|Tug1_chr11_3648649 |
CTCCAACTGGGTCCTAGCAGGCCCCGAGGGGTGGCGCCAGGCCTCCAGGCCAGCCCCGGCCACCCCAGCTTCCCCCGCCG CCTCCCCGGGAGCGGGGCGGAGTTGCCCGCCGCCGCCAACCACCCGGACGACC |
| mm4_exon_Slc22a21_chr11_53979812 |
GAAGAAGATGAGGCGCTGGAAGGTCCCCCACTCGCCCAGGAAGGCGGTCACCTCGTCGTAGTCAAGCATAGAGTCGGCAG C |
| mm4_intron_Gli2_chr1_118856978 |
TGTTCTTTTCCCCATCATCATCACCCCCCACCCTCCCAGGAATTCTGGATCTCTCCTTTAGACAAAGTGTCCTAGAAGTG GTCTGACATCACTAGATGACTGTAGTCCCTATTGCTTCTTCACTGCCTCAGCTCACA |
| mm4_intron_Ubtf_chr11_102318757 |
CAGCGGAAGAGTCACTCTGGGAGCCGGTTCAATCCAGCCCGGCATGGCTATCCAGGCCTTCCCGGGCGCGTGGAGGACGG AGATTGGCGCCGCCAGAGAGCAACCCCTTTCC |
| mm4_intergenic_n-R5s172|Gm26486_chr5_53355431 |
CTCACCGGCCAGAGTGTATCCCTCCGGAGGACTGCAGGAATTCAGTTTATTACTGTCCCTATGGTCCCCAAAGCTCCAGG GAAGTTGGCTGGGTTGATAGAGGCACAAAGGATCCATGGG |
| mm4_intron_Tbc1d2_chr4_46646037 |
CGTTCATTCCTGGTCCTGCTTCTTGGCAGAGAGCTCCTCCCACGCTCCCAGCAAGAGGGGGAAAGCCTGGCTATCAGATG CTTGGGTCTGTGG |
| mm3_intergenic_Ubr5|Odf1_chr15_38178301 |
CCAGAAGCAGAAGTCCAGCAGGTCAGGCCTGGTGCTCAGGCTTCCTGGGAGCGTGGAGAAGCCTTAGAGCAAAGGAACGG ACATTTTAGATGATTTTCTCGGGGTGGGG |
| mm4_exon_6030419C18Rik_chr9_58489037 |
CGAGCGTGGTGAGGAGATTTTTGGGGATTTTTTTTTTTTGCCCCCTTCCCCGGAGCGCGGAGGCCCCGCCCCCTCCCACC GCCCAGATGTCTCCGGTCACCG |