CRISPOR

DNA break repair outcome predictions

Guide: GGATTTCTATGAAGAAATCG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGTAGCTCAGACACCGAATCGGATTTCTATGAAGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACGCCGA`	(Wild-type sequence)
201	`ACACCGAATCGGATTTCTATGAAG--------GTGAGCTGCACCCCAGACTGCGCCACCG`	`8 bp deleted → frameshift`
198	`ACACCGAATCGGATTTCTATGA--------------GCTGCACCCCAGACTGCGCCACCG`	`14 bp deleted → frameshift`
173	`ACACCGAATCGGATTTCTATGAAGA-----------GCTGCACCCCAGACTGCGCCACCG`	`11 bp deleted → frameshift`
156	`ACACCGAATCGGATTTCTATGAAG-------------CTGCACCCCAGACTGCGCCACCG`	`13 bp deleted → frameshift`
140	`ACACCGAATCGG---------------------TGAGCTGCACCCCAGACTGCGCCACCG`	`21 bp deleted → no frameshift`
120	`ACACCGAATCGGATTTCTATGAAGAAATCG------------------------CCACCG`	`24 bp deleted → no frameshift`
120	`ACACCGAATCGGATTTCTATGAAGA------------------------CTGCGCCACCG`	`24 bp deleted → no frameshift`
116	`ACACCGAATCGGATTTCTATG-------------------CACCCCAGACTGCGCCACCG`	`19 bp deleted → frameshift`
105	`ACACCGAATCGGATTTCT---------------------GCACCCCAGACTGCGCCACCG`	`21 bp deleted → no frameshift`
99	`ACACC---------------------------------------CCAGACTGCGCCACCG`	`39 bp deleted → no frameshift`
95	`ACACCGAATCGGA-----------------------GCTGCACCCCAGACTGCGCCACCG`	`23 bp deleted → frameshift`
89	`ACACCGAATCGGATTTCTATGAAGAAATCG------------------------------`	`30 bp deleted → no frameshift`
77	`ACACCGAATCGGATTTCTATGA---------------------------CTGCGCCACCG`	`27 bp deleted → no frameshift`
70	`ACACCGA-----------------------------GCTGCACCCCAGACTGCGCCACCG`	`29 bp deleted → frameshift`
63	`ACACCGAATCGGATTTCTATG-------------------------------CGCCACCG`	`31 bp deleted → frameshift`
60	`ACACCG------------------------------------------------------`	`54 bp deleted → no frameshift`
57	`ACACCGAATCGGATTTCT---------------------------------GCGCCACCG`	`33 bp deleted → no frameshift`
54	`ACACC----------------------------------------CAGACTGCGCCACCG`	`40 bp deleted → frameshift`
51	`ACACC-----------------------------------------AGACTGCGCCACCG`	`41 bp deleted → frameshift`
49	`ACACCGAATCGGA------------------------------------CTGCGCCACCG`	`36 bp deleted → no frameshift`
46	`ACACCGAATCG-------------------------------------------CCACCG`	`43 bp deleted → frameshift`
38	`AC-----------------------------------------CCCAGACTGCGCCACCG`	`41 bp deleted → frameshift`
36	`ACACCGA------------------------------------------CTGCGCCACCG`	`42 bp deleted → no frameshift`
36	`ACACCG------------------------------------------------CCACCG`	`48 bp deleted → no frameshift`
34	`ACACCGAATCG-------------------------------------------------`	`49 bp deleted → frameshift`
33	`ACA--------------------------------------------GACTGCGCCACCG`	`44 bp deleted → frameshift`
31	`ACACC---------------------------------------------------ACCG`	`51 bp deleted → no frameshift`
30	`ACAC----------------------------------------------TGCGCCACCG`	`46 bp deleted → frameshift`
27	`AC------------------------------------------------TGCGCCACCG`	`48 bp deleted → no frameshift`
18	`AC--------------------------------------------------------CG`	`56 bp deleted → frameshift`

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