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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314226_F | TCGTCGGCAGCGTCAGAAATCGAGGTGAGCTGCA | 59.3 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314226_R | GTCTCGTGGGCTCGGGAAGGGAACTGTGGGTGGAG | 59.9 | 1.00 |
| mm4_intergenic_Gm12442|E130309F12Rik_chr4_49039155_F | Primer3: not found at this Tm | N.d. | 0.68 |
| mm4_intergenic_Gm12442|E130309F12Rik_chr4_49039155_R | Primer3: not found at this Tm | N.d. | 0.68 |
| mm4_intergenic_Retnlb|Retnla_chr16_48838852_F | Primer3: not found at this Tm | N.d. | 0.62 |
| mm4_intergenic_Retnlb|Retnla_chr16_48838852_R | Primer3: not found at this Tm | N.d. | 0.62 |
| mm4_exon_Adcy3_chr12_4200930_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_exon_Adcy3_chr12_4200930_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Gm26772|Zmiz1_chr14_25560071_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Gm26772|Zmiz1_chr14_25560071_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm3_intergenic_Gm24304|Gm22974_chr5_80550465_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3_intergenic_Gm24304|Gm22974_chr5_80550465_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Thsd7b_chr1_129895331_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intron_Thsd7b_chr1_129895331_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Nudt10|Bmp15_chrX_6312445_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Nudt10|Bmp15_chrX_6312445_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42359948_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42359948_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm11042|Kcnh5_chr12_74836801_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm11042|Kcnh5_chr12_74836801_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intron_Zc3h12b_chrX_95868422_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Zc3h12b_chrX_95868422_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Dcx|Gm15048_chrX_144008163_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Dcx|Gm15048_chrX_144008163_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Zbtb46|Abhd16b_chr2_181480486_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Zbtb46|Abhd16b_chr2_181480486_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm24467|Gm13456_chr2_40501254_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm24467|Gm13456_chr2_40501254_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm3_intron_Gm2716_chr8_88077442_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm3_intron_Gm2716_chr8_88077442_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Psg29|Gm24520_chr7_17609682_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Psg29|Gm24520_chr7_17609682_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Pold2|Myl7_chr11_5889691_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Pold2|Myl7_chr11_5889691_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm6917|Gm6910_chr13_65916723_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm6917|Gm6910_chr13_65916723_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm15501|Gm5899_chr7_94132075_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm15501|Gm5899_chr7_94132075_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Cant1_chr11_118410749_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Cant1_chr11_118410749_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Umod_chr7_119474613_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Umod_chr7_119474613_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm22594|1110002J07Rik_chr10_66596266_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm22594|1110002J07Rik_chr10_66596266_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm23043|Gm24943_chr4_51747087_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm23043|Gm24943_chr4_51747087_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Mtpn|Gm23273_chr6_35846130_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Mtpn|Gm23273_chr6_35846130_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm11197|Fam58b_chr11_78741008_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm11197|Fam58b_chr11_78741008_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm11418|5530401A14Rik_chr11_81649160_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm11418|5530401A14Rik_chr11_81649160_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9162|Scgb2b19_chr7_33212656_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9162|Scgb2b19_chr7_33212656_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9135|Scgb2b12_chr7_32249779_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9135|Scgb2b12_chr7_32249779_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Grm5_chr7_88068777_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Grm5_chr7_88068777_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Nudt9_chr5_104062132_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Nudt9_chr5_104062132_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Agbl1_chr7_76690337_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Agbl1_chr7_76690337_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Tmco5b|Fmn1_chr2_113313037_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Tmco5b|Fmn1_chr2_113313037_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Slitrk1|Gm25901_chr14_109249073_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Slitrk1|Gm25901_chr14_109249073_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Pdcd1|Gm23389_chr1_94514129_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Pdcd1|Gm23389_chr1_94514129_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Olfr1008|Olfr1009_chr2_85691498_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Olfr1008|Olfr1009_chr2_85691498_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_71988489_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_71988489_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17810299_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17810299_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm6984|Htr2a_chr14_74258090_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm6984|Htr2a_chr14_74258090_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5506|Gm5839_chr18_48494015_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5506|Gm5839_chr18_48494015_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26972|Gm23581_chr18_63487023_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26972|Gm23581_chr18_63487023_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm24686|Zfp804b_chr5_6747678_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm24686|Zfp804b_chr5_6747678_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23891|1700018B24Rik_chr3_48255047_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23891|1700018B24Rik_chr3_48255047_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23443|Gm12640_chr4_93555526_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23443|Gm12640_chr4_93555526_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm22347|Gm23764_chr14_102775657_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm22347|Gm23764_chr14_102775657_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm14776|Il1rapl1_chrX_87861306_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm14776|Il1rapl1_chrX_87861306_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm14469|Gm14465_chr2_79110289_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm14469|Gm14465_chr2_79110289_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm12015|Gm23959_chr11_17536995_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm12015|Gm23959_chr11_17536995_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm11218|Gm11219_chr4_64872759_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm11218|Gm11219_chr4_64872759_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Glt1d1|Tmem132d_chr5_127739491_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Glt1d1|Tmem132d_chr5_127739491_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_4931417E11Rik|Gm26179_chr6_74287059_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_4931417E11Rik|Gm26179_chr6_74287059_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Fbxl17_chr17_63499905_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Fbxl17_chr17_63499905_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intron_Peg3_chr7_6730093_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intron_Peg3_chr7_6730093_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5394|Gm25825_chrX_121205818_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5394|Gm25825_chrX_121205818_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm22797|Robo1_chr16_72288918_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm22797|Robo1_chr16_72288918_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Frk|Gm26341_chr10_34789185_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Frk|Gm26341_chr10_34789185_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm26076|Gm25519_chr3_110906895_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm26076|Gm25519_chr3_110906895_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm23838|Gm25233_chr5_6208472_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm23838|Gm25233_chr5_6208472_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26119|Fzd8_chr18_8780700_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm26119|Fzd8_chr18_8780700_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Pcdh17|Gm23926_chr14_85548658_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Pcdh17|Gm23926_chr14_85548658_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Olfr746|Olfr747_chr14_50678755_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Olfr746|Olfr747_chr14_50678755_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Lrp1b|Gm13462_chr2_40936620_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Lrp1b|Gm13462_chr2_40936620_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm24464|Psg19_chr7_18770993_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm24464|Psg19_chr7_18770993_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_AC161571.1|Gm25956_chr7_54042862_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_AC161571.1|Gm25956_chr7_54042862_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm22016|Cdc73_chr1_143474468_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm22016|Cdc73_chr1_143474468_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ythdf3|Gm26485_chr3_16230946_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Ythdf3|Gm26485_chr3_16230946_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Il12rb2|Gm4761_chr6_67408549_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Il12rb2|Gm4761_chr6_67408549_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm24297|Gm22911_chr10_38268388_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm24297|Gm22911_chr10_38268388_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm14854|Gm7117_chrX_105709936_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm14854|Gm7117_chrX_105709936_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cntnap2|Gm25161_chr6_46422135_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cntnap2|Gm25161_chr6_46422135_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm15762|B230104C08Rik_chr6_147921247_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm15762|B230104C08Rik_chr6_147921247_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Atp2b3|Dusp9_chrX_73595554_F | TCGTCGGCAGCGTCGCCTGGAAAGGGCTCTGATA | 59.1 | 0.15 |
| mm4_intergenic_Atp2b3|Dusp9_chrX_73595554_R | GTCTCGTGGGCTCGGGCTCACTGTCCCCACAATACT | 59.7 | 0.15 |
| mm4_intergenic_Tspyl5|Mtdh_chr15_33709946_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Tspyl5|Mtdh_chr15_33709946_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm5272|1700008P02Rik_chr3_6549855_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm5272|1700008P02Rik_chr3_6549855_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_B430218F22Rik/Mrps30|Fgf10_chr13_118641028_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_B430218F22Rik/Mrps30|Fgf10_chr13_118641028_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Car1|Car3_chr3_14816654_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Car1|Car3_chr3_14816654_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intron_Lmx1b_chr2_33567117_F | TCGTCGGCAGCGTCGATAGCAGTGGGTCATGGGG | 59.8 | 0.14 |
| mm3_intron_Lmx1b_chr2_33567117_R | GTCTCGTGGGCTCGGGCGAAGAGCTTTCAAGGCAT | 59.1 | 0.14 |
| mm4_intergenic_Irf2bp1|Foxa3_chr7_19012075_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Irf2bp1|Foxa3_chr7_19012075_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Trpc7_chr13_56806045_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Trpc7_chr13_56806045_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_4933428C19Rik|Gm12391_chr4_40624437_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_4933428C19Rik|Gm12391_chr4_40624437_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm24681|Gm23591_chr16_68887145_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm24681|Gm23591_chr16_68887145_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm9144|Apob_chr12_7948826_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm9144|Apob_chr12_7948826_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Myo3a_chr2_22554142_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Myo3a_chr2_22554142_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22827|Gm22181_chr8_112113034_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22827|Gm22181_chr8_112113034_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Armc2|Gm9855_chr10_42038919_F | TCGTCGGCAGCGTCCCTTCCACACAAGCTCCCTT | 59.8 | 0.12 |
| mm4_intergenic_Armc2|Gm9855_chr10_42038919_R | GTCTCGTGGGCTCGGGGAGAAGTCGGGGAAGCAAA | 59.9 | 0.12 |
| mm4_intron_Cntn5_chr9_10028266_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Cntn5_chr9_10028266_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm9578|Gm22891_chr14_81535134_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm9578|Gm22891_chr14_81535134_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6433|Gm16035_chr5_141285341_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm6433|Gm16035_chr5_141285341_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Col6a3|Mlph_chr1_90906987_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Col6a3|Mlph_chr1_90906987_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11901|Gm25173_chr4_27630707_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11901|Gm25173_chr4_27630707_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm2_intergenic_Glrx3|Gm25798_chr7_137784584_F | TCGTCGGCAGCGTCAAAAGGCCGGAGAAGACAGC | 60.6 | 0.10 |
| mm2_intergenic_Glrx3|Gm25798_chr7_137784584_R | GTCTCGTGGGCTCGGAACAAGTCCCCACTAGTGCG | 59.9 | 0.10 |
| mm4_intron_Oxct1_chr15_4138753_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Oxct1_chr15_4138753_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Gabpb1_chr2_126674722_F | TCGTCGGCAGCGTCCAACAGCACACTCAACACCG | 59.9 | 0.10 |
| mm4_intron_Gabpb1_chr2_126674722_R | GTCTCGTGGGCTCGGCCATTCTCAGTGGCCGAGAC | 60.4 | 0.10 |
| mm4_exon_Megf11_chr9_64501521_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Megf11_chr9_64501521_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Hmgb1-ps2|Gm15061_chrX_146203866_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Hmgb1-ps2|Gm15061_chrX_146203866_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8677|Gm4201_chr7_22538302_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8677|Gm4201_chr7_22538302_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8653|Gm8453_chr7_22204769_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8653|Gm8453_chr7_22204769_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm5726|Gm4141_chr7_20549172_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm5726|Gm4141_chr7_20549172_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11988|Gm11989_chr11_8134120_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11988|Gm11989_chr11_8134120_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10975206_F | TCGTCGGCAGCGTCAAAGGAGTCCCATGTGCAGG | 59.9 | 0.09 |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10975206_R | GTCTCGTGGGCTCGGGTCCCCACAGTGGCTTCTTT | 60.1 | 0.09 |
| mm4_intron_Kcns1_chr2_164170491_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Kcns1_chr2_164170491_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Chst11|1700025N21Rik_chr10_83121180_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Chst11|1700025N21Rik_chr10_83121180_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm25637|Aqp3_chr4_41070521_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm25637|Aqp3_chr4_41070521_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Exoc6b_chr6_84937539_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Exoc6b_chr6_84937539_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Rpl21-ps5|Gm24508_chr16_83429550_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Rpl21-ps5|Gm24508_chr16_83429550_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Pkhd1|Mir206_chr1_20650507_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Pkhd1|Mir206_chr1_20650507_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Supt3_chr17_44926946_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Supt3_chr17_44926946_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cyp2c69_chr19_39853656_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cyp2c69_chr19_39853656_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Usp15/AC158802.1|Fam19a2_chr10_123246875_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Usp15/AC158802.1|Fam19a2_chr10_123246875_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Phkb|Gm23904_chr8_86094899_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Phkb|Gm23904_chr8_86094899_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Olfr473|Olfr474_chr7_107949340_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Olfr473|Olfr474_chr7_107949340_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm26108|Pou3f4_chrX_110706028_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm26108|Pou3f4_chrX_110706028_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25144|Gm23147_chr3_5922889_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25144|Gm23147_chr3_5922889_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm4991|Gm7134_chrX_110304704_F | TCGTCGGCAGCGTCAGGCAAACTCAACCTCACCT | 59.1 | 0.07 |
| mm4_intergenic_Gm4991|Gm7134_chrX_110304704_R | GTCTCGTGGGCTCGGAAAATGCTTCTCTGCGAGCT | 58.1 | 0.07 |
| mm4_intergenic_Zcchc6|Gas1_chr13_60031795_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Zcchc6|Gas1_chr13_60031795_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_AI846148|Rab11b-ps2_chr19_7392952_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_AI846148|Rab11b-ps2_chr19_7392952_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Mrgpra3|Gm22427_chr7_47614715_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Mrgpra3|Gm22427_chr7_47614715_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Sh3tc1_chr5_35699835_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Sh3tc1_chr5_35699835_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tfdp1_chr8_13369699_F | TCGTCGGCAGCGTCGGGTCCCTGAGCAGAGAGA | 59.9 | 0.04 |
| mm4_intron_Tfdp1_chr8_13369699_R | GTCTCGTGGGCTCGGATAGAGCCCTGGTGTTGGAC | 59.0 | 0.04 |
| mm4_intergenic_Galnt14|Ehd3_chr17_73739937_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Galnt14|Ehd3_chr17_73739937_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tacc2_chr7_130622654_F | TCGTCGGCAGCGTCTACCACAGCACCAGCAAACA | 60.1 | 0.03 |
| mm4_exon_Tacc2_chr7_130622654_R | GTCTCGTGGGCTCGGGCCACACAGACAACTTGCTG | 59.9 | 0.03 |
| mm4_exon_Dnajb5_chr4_42949874_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Dnajb5_chr4_42949874_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Hsd11b1|1600010F14Rik_chr1_193251629_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Hsd11b1|1600010F14Rik_chr1_193251629_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tbx19|Sft2d2_chr1_165165247_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tbx19|Sft2d2_chr1_165165247_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_4930529M08Rik_chr2_146025195_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_4930529M08Rik_chr2_146025195_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ankef1|Snap25_chr2_136678797_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ankef1|Snap25_chr2_136678797_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nucb2_chr7_116539058_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nucb2_chr7_116539058_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dhodh_chr8_109600932_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dhodh_chr8_109600932_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Atcay_chr10_81230292_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Atcay_chr10_81230292_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Alox5ap_chr5_149285701_F | TCGTCGGCAGCGTCTTCGCACAGCCTTGATTCCT | 59.9 | 0.00 |
| mm4_intron_Alox5ap_chr5_149285701_R | GTCTCGTGGGCTCGGGCCTGGGAAGTCAGACCTAC | 59.4 | 0.00 |
| mm4_intron_Aatk_chr11_120020728_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Aatk_chr11_120020728_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Prlhr|Cacul1_chr19_60486341_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Prlhr|Cacul1_chr19_60486341_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nkx1-1|Fam53a_chr5_33483866_F | TCGTCGGCAGCGTCTGCTTCTTGGTCAGCCCTG | 59.9 | 0.00 |
| mm4_intergenic_Nkx1-1|Fam53a_chr5_33483866_R | GTCTCGTGGGCTCGGATGGCAGCTCTACCCTCAGT | 60.3 | 0.00 |
| mm4_intergenic_Grxcr2|Sh3rf2_chr18_42023483_F | TCGTCGGCAGCGTCGGATGGTCTCAAGCTTTGCC | 59.1 | 0.00 |
| mm4_intergenic_Grxcr2|Sh3rf2_chr18_42023483_R | GTCTCGTGGGCTCGGGTCACACTCAGCATCTGCCT | 60.0 | 0.00 |
| mm4_intergenic_Gm23674|Gm26106_chr18_26602090_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23674|Gm26106_chr18_26602090_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11466|Gm11467_chr2_166204930_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11466|Gm11467_chr2_166204930_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Nod2_chr8_88664415_F | TCGTCGGCAGCGTCCGCTCCTCAGGAAGTTCGTT | 60.0 | 0.00 |
| mm4_exon_Nod2_chr8_88664415_R | GTCTCGTGGGCTCGGGTGGCTTGGATCAGCTGGAT | 60.1 | 0.00 |
| mm4_exon_Mocos_chr18_24653709_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Mocos_chr18_24653709_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Megf8_chr7_25353741_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Megf8_chr7_25353741_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314226 |
AGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACGCCGAGTACCAGCACAGCAAAGGTAGCCACCTTGCCC CTCCGCTCCCCGGTCCGCCCACTCCACCCACAGTTCCCTTC |
| mm4_intergenic_Atp2b3|Dusp9_chrX_73595554 |
GCCTGGAAAGGGCTCTGATAGTTCCAGGTGCAGCAGACTGTGGCTGCTTCTGCAATAACCCCTTATTTTCTCCCAATTTT TTGTAATTTAACAGATCACAAGGTCACCTCAACAAATCAGTATTGTGGGGACAGTGAGC |
| mm3_intron_Lmx1b_chr2_33567117 |
GATAGCAGTGGGTCATGGGGGAGGAACAGACCCACAATAAGCAAAAGGGGGGCGTTGGCCCCAGACTGCCCCACCCAGAA CCCCTCACCTTCCGACAGGGCTTGGAGGAGACCTCAAAGGATGCCTTGAAAGCTCTTCGC |
| mm4_intergenic_Armc2|Gm9855_chr10_42038919 |
CCTTCCACACAAGCTCCCTTCATTCTCCCTAGCTTCCTGCCCGCTCGCTCCCCGCTCACTCCCTGCTTTTCTCACACCAC AGACTTCGACATCAGGAACGGCCTTGACTTTGCTTCCCCGACTTCTCC |
| mm2_intergenic_Glrx3|Gm25798_chr7_137784584 |
AAAAGGCCGGAGAAGACAGCAGCTCCTACTTCAGACTGCTCCACAGGGAACAAATGGAGAGGAGGGCGCACTAGTGGGGA CTTGTT |
| mm4_intron_Gabpb1_chr2_126674722 |
CAACAGCACACTCAACACCGATCTGTGCCCAGGCAAGACATGACCGACGAAACTCGCGGCTTCTTTCCGCACCCGCGCTC GCGTGGGTTCCGGTGGCGAAGTCTCCGGGGCAAAAGTCTCGGCCACTGAGAATGG |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10975206 |
AAAGGAGTCCCATGTGCAGGAGAGGAGGAAACTGGCTCTCCAGACAGTTAGCCCTCTGGCCCACAGACTGCAGCACCGGA CACAGGCAAGGAGTCCAAAACCAACCAGGACAGTGGAGAAAAGAAGCCACTGTGGGGAC |
| mm4_intergenic_Gm4991|Gm7134_chrX_110304704 |
AGGCAAACTCAACCTCACCTCTTACCTTAGGTTAAGACATACAGTAAGTTCAAACCACAGACTGAGACACTGAGAACAGC TCGCAGAGAAGCATTTT |
| mm4_intron_Tfdp1_chr8_13369699 |
GGGTCCCTGAGCAGAGAGAGCGCAGGGCTCCCATCTCACCTCTGACTGGGCCACAGAGAACACCGGTCACTAATACGACA CAAACGCTTAGAAAGTGTCAGAAGATCTGATCTCTACCAGGTCCAACACCAGGGCTCTAT |
| mm4_exon_Tacc2_chr7_130622654 |
TACCACAGCACCAGCAAACAGTGGCTCCTGGAAAGAGACTCTGGACACCAGCGATGCTCAGAGGCAACCACAGACAGGGA CATCGGGAACTGAGCTCCAGCAAGTTGTCTGTGTGGC |
| mm4_intron_Alox5ap_chr5_149285701 |
TTCGCACAGCCTTGATTCCTAGGGGTGTTTTAGCACACCGGGCTGTACACTGCTTGCACCTGCACGCCCAGCTGTTCTCC GTGGTGGAGTCTGGGGCGGCAATAACTTTTTAGGTAGGTCTGACTTCCCAGGC |
| mm4_intergenic_Nkx1-1|Fam53a_chr5_33483866 |
TGCTTCTTGGTCAGCCCTGGCTCCCAGTGGCCCACTCTGAGGCAGGCAGAGACACAATATGGACGCAGTGTGTGAATTTA AGGAACATTGCACAATTCTACAGCTAAAGATCGAGTCTTCACTGAGGGTAGAGCTGCCAT |
| mm4_intergenic_Grxcr2|Sh3rf2_chr18_42023483 |
GGATGGTCTCAAGCTTTGCCCTTCCTGGTGGCCCACTCTGTGGTATTGGCTGAATTTTGACATTTATTTCTGATCCTTAA CCAAACTACTCTCTTCCATCCCAATTCTTGGTAGTTCTAGAGGCAGATGCTGAGTGTGAC |
| mm4_exon_Nod2_chr8_88664415 |
CGCTCCTCAGGAAGTTCGTTCGCACAGAGTTGCAACTGAAGGGCTTCTCTGAAGAGGGCATCCAACTGTACCTGAGAAAG CACCACCGGGAACCTGGGGTGGCAGACCGCCTCATCCAGCTGATCCAAGCCAC |