CRISPOR

DNA break repair outcome predictions

Guide: GATTCGGTGTCTGAGCTACT AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGATTTCTTCATAGAAATCCGATTCGGTGTCTGAGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACT`	(Wild-type sequence)
230	`ATAGAAATCCGATTCGGTGTCTGAGC-----------CCTTGGCCAGAGAGGCTGTCCGC`	`11 bp deleted → frameshift`
224	`ATAGAAATCCGATTCGGT----------------TGCCCTTGGCCAGAGAGGCTGTCCGC`	`16 bp deleted → frameshift`
211	`ATAGAAATCCGATTCGGTGTCTGAG-------GTTGCCCTTGGCCAGAGAGGCTGTCCGC`	`7 bp deleted → frameshift`
182	`ATAGAAATCCGATTCGGTGTCTGAGCTACT----------TGGCCAGAGAGGCTGTCCGC`	`10 bp deleted → frameshift`
162	`ATAGAAATCCGATTCGGTGTCTGAGC------------------CAGAGAGGCTGTCCGC`	`18 bp deleted → no frameshift`
156	`ATAGAAATCCGATTCGGTGTCTGAGCT-------------TGGCCAGAGAGGCTGTCCGC`	`13 bp deleted → frameshift`
156	`ATAGAAATCCGATTCGGTGTCTG-------------CCCTTGGCCAGAGAGGCTGTCCGC`	`13 bp deleted → frameshift`
150	`ATAGAAATCCGATTCGGTGTCTGAG------------------------AGGCTGTCCGC`	`24 bp deleted → no frameshift`
149	`ATAGAAATCCGATTCGGTGT--------------TGCCCTTGGCCAGAGAGGCTGTCCGC`	`14 bp deleted → frameshift`
136	`ATAGAAATCCGATTCGGTGTCTGAG--------------------------GCTGTCCGC`	`26 bp deleted → frameshift`
129	`ATAGAAATCCGATTCGGTGTCTGAGCT---------------------------GTCCGC`	`27 bp deleted → no frameshift`
128	`ATAGAAATCCGATTCGGTG-----------------CCCTTGGCCAGAGAGGCTGTCCGC`	`17 bp deleted → frameshift`
122	`ATAGAAATCCGATTCGGTGTCT------------------TGGCCAGAGAGGCTGTCCGC`	`18 bp deleted → no frameshift`
116	`ATAGAAATCCGATTCGGTGTCTG-------------------GCCAGAGAGGCTGTCCGC`	`19 bp deleted → frameshift`
109	`ATAGAAATCCGATTCGG--------------------------CCAGAGAGGCTGTCCGC`	`26 bp deleted → frameshift`
101	`ATAGAAATCCGATTCGGTGTCTG--------------------------------TCCGC`	`32 bp deleted → frameshift`
99	`ATAGAAATCCGATTCGGTGTCTGAG----------------------AGAGGCTGTCCGC`	`22 bp deleted → frameshift`
99	`ATAGAAATCCGATTCGGTGTC------------------------------------CGC`	`36 bp deleted → no frameshift`
98	`ATAGAAATCC----------------------------CTTGGCCAGAGAGGCTGTCCGC`	`28 bp deleted → frameshift`
95	`ATAGAAATCCGATTCGGTG-----------------------GCCAGAGAGGCTGTCCGC`	`23 bp deleted → frameshift`
94	`ATAGAAATCC-----------------------------TTGGCCAGAGAGGCTGTCCGC`	`29 bp deleted → frameshift`
90	`ATAGAAATCCGATTCGGTGTCTGAGCTACT------------------------GTCCGC`	`24 bp deleted → no frameshift`
74	`ATAG----------------------------GTTGCCCTTGGCCAGAGAGGCTGTCCGC`	`28 bp deleted → frameshift`
73	`ATAGAAATCCGATTCGGTGTCTGAGC----------------------------------`	`34 bp deleted → frameshift`
70	`ATAGAAATCCGATT---------------------GCCCTTGGCCAGAGAGGCTGTCCGC`	`21 bp deleted → no frameshift`
69	`ATAGAAATCCGATTCGG-----------------------------------CTGTCCGC`	`35 bp deleted → frameshift`
69	`ATAGAAATCC-----------------------------------AGAGAGGCTGTCCGC`	`35 bp deleted → frameshift`
63	`ATAGAAATCCG------------------------------------------------C`	`48 bp deleted → no frameshift`
51	`ATAGAAATCCGATT---------------------------GGCCAGAGAGGCTGTCCGC`	`27 bp deleted → no frameshift`
49	`ATAGAAATCCGA------------------------------------GAGGCTGTCCGC`	`36 bp deleted → no frameshift`
46	`ATAGAAATCCGATTCG-------------------------------------------C`	`43 bp deleted → frameshift`
46	`ATAGA-------------------------------------------GAGGCTGTCCGC`	`43 bp deleted → frameshift`
45	`ATAGAAATCCGATTCGGT--------------------------------------CCGC`	`38 bp deleted → frameshift`
45	`ATAGAAATCCGA--------------------------------------GGCTGTCCGC`	`38 bp deleted → frameshift`
42	`ATAGA---------------------------------------------GGCTGTCCGC`	`45 bp deleted → no frameshift`
36	`ATAGAAATCCGATTC------------------------------------------CGC`	`42 bp deleted → no frameshift`
28	`ATAG-----------------------------------------------GCTGTCCGC`	`47 bp deleted → frameshift`

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