CRISPOR

DNA break repair outcome predictions

Guide: AAGTCGGAGGGCCGGCTCGC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTT`	(Wild-type sequence)
603	`CGCTCCCGGGAAGTCGGAGGGCCGGCTC--------AGGCAACCGTGGGGCACCATCTTC`	`8 bp deleted → frameshift`
439	`CGCTCCCGGGAAGTCGGAGGGC------------TCAGGCAACCGTGGGGCACCATCTTC`	`12 bp deleted → no frameshift`
327	`CGCTCCCGGGAAGTCGGAGGGCCGGCTCGC----TCAGGCAACCGTGGGGCACCATCTTC`	`4 bp deleted → frameshift`
298	`CGCTCCCGGGAAGTCGGAGGGCCGGC--------------AACCGTGGGGCACCATCTTC`	`14 bp deleted → frameshift`
282	`CGCTCCCGGGAAGTCGGAGGGCCGG-------GCTCAGGCAACCGTGGGGCACCATCTTC`	`7 bp deleted → frameshift`
244	`CGCTCCCGGGAAGTCGGAGGGC------------------AACCGTGGGGCACCATCTTC`	`18 bp deleted → no frameshift`
242	`CGCTCCCGGGAAGTCGGAGGGCCGGCTCGC----------AACCGTGGGGCACCATCTTC`	`10 bp deleted → frameshift`
230	`CGCTCCCGGGAAGTCGGAGGG-----------GCTCAGGCAACCGTGGGGCACCATCTTC`	`11 bp deleted → frameshift`
210	`CGCTCCCGGGAAGTCGGAGGGCCG---------------------TGGGGCACCATCTTC`	`21 bp deleted → no frameshift`
208	`CGCTCCCGGGAAGTCGGAGG-------------CTCAGGCAACCGTGGGGCACCATCTTC`	`13 bp deleted → frameshift`
193	`CGCTCCCGGGAAGTCGGAGG-------------------CAACCGTGGGGCACCATCTTC`	`19 bp deleted → frameshift`
190	`CGCTCCCGGG-----------------------CTCAGGCAACCGTGGGGCACCATCTTC`	`23 bp deleted → frameshift`
188	`CGCTCCCGGGAAGTCGG---------------GCTCAGGCAACCGTGGGGCACCATCTTC`	`15 bp deleted → no frameshift`
188	`CGCTCCCGGGAAGTCGGAGGGC-----------------------------ACCATCTTC`	`29 bp deleted → frameshift`
179	`CGCTCCCGGGAAGTCGGAGGGCCGGCTCG----------------TGGGGCACCATCTTC`	`16 bp deleted → frameshift`
179	`CGCTCCCGGGAAGTCGG----------------CTCAGGCAACCGTGGGGCACCATCTTC`	`16 bp deleted → frameshift`
172	`CGCTCCCGGGAAGTCGGAGGGCCGGC-------------------------ACCATCTTC`	`25 bp deleted → frameshift`
148	`CGCTC-------------------------------AGGCAACCGTGGGGCACCATCTTC`	`31 bp deleted → frameshift`
148	`CGCTCCCGGGAAGTCGGAGGG----------------------------GCACCATCTTC`	`28 bp deleted → frameshift`
140	`CGCTCCCGGGAAGTCGGAGGGCCGGCTCGC---------------------ACCATCTTC`	`21 bp deleted → no frameshift`
133	`CGCTCCCGGGAAGTCGG----------------------CAACCGTGGGGCACCATCTTC`	`22 bp deleted → frameshift`
133	`CGCTCCCGGG----------------------GCTCAGGCAACCGTGGGGCACCATCTTC`	`22 bp deleted → frameshift`
126	`CGCTCCCGGGAAGTCGGAGGGCCGG-----------------------GGCACCATCTTC`	`23 bp deleted → frameshift`
120	`CGCTCCCGGGAAGTCGGAGGGCCGG------------------------GCACCATCTTC`	`24 bp deleted → no frameshift`
120	`CGCTCCCGG------------------------CTCAGGCAACCGTGGGGCACCATCTTC`	`24 bp deleted → no frameshift`
105	`CGCTCCCGGGAAGTC---------------------AGGCAACCGTGGGGCACCATCTTC`	`21 bp deleted → no frameshift`
103	`CGCTCCCGGGAAGTCGGAGGG---------------------------GGCACCATCTTC`	`27 bp deleted → no frameshift`
94	`CGCTCCCG-------------------------------------TGGGGCACCATCTTC`	`37 bp deleted → frameshift`
94	`CGCTCCCGGGAAGTCG-----------------------------TGGGGCACCATCTTC`	`29 bp deleted → frameshift`
94	`CGCTCCCGGG-----------------------------CAACCGTGGGGCACCATCTTC`	`29 bp deleted → frameshift`
89	`CGCTCCCGGGAAGTCGGAGG------------------------------CACCATCTTC`	`30 bp deleted → no frameshift`
89	`CGCTCCCGG------------------------------CAACCGTGGGGCACCATCTTC`	`30 bp deleted → no frameshift`
86	`CGCTCCCGGGAAG-------------------------GCAACCGTGGGGCACCATCTTC`	`25 bp deleted → frameshift`
85	`CGCTCCCGGG---------------------------------------GCACCATCTTC`	`39 bp deleted → no frameshift`
84	`CGCTCCCGGGAAGTCGGAGGGCC-------------------------------ATCTTC`	`31 bp deleted → frameshift`
84	`CGCTCCCGGGAAGTCGG-------------------------------GGCACCATCTTC`	`31 bp deleted → frameshift`
81	`CGCTCCCGGG----------------------------------------CACCATCTTC`	`40 bp deleted → frameshift`
80	`CGCTCCCGGGAAGTCGG--------------------------------GCACCATCTTC`	`32 bp deleted → frameshift`
76	`CGCTCCCGGGAAGTCGG---------------------------------CACCATCTTC`	`33 bp deleted → no frameshift`
70	`CGCTCCCGGGAAGTCGGAGGGCCGGCTC-----------------------------TTC`	`29 bp deleted → frameshift`
63	`CGCTCCCGGGAAGTCGGAGGGCCGGCT-------------------------------TC`	`31 bp deleted → frameshift`
62	`CGC-------------------------------------AACCGTGGGGCACCATCTTC`	`37 bp deleted → frameshift`
60	`CGCTCCCGGGAAGTCGGAGGGCCGGCTC--------------------------------`	`32 bp deleted → frameshift`
60	`CGCTCCCGGGAAGT--------------------------------GGGGCACCATCTTC`	`32 bp deleted → frameshift`
60	`CGCTCCCGGG--------------------------------------GGCACCATCTTC`	`38 bp deleted → frameshift`
60	`CGCTCC--------------------------------------GTGGGGCACCATCTTC`	`38 bp deleted → frameshift`
51	`CGCTCCCGG-----------------------------------------CACCATCTTC`	`41 bp deleted → frameshift`
46	`CG-------------------------------------------TGGGGCACCATCTTC`	`43 bp deleted → frameshift`
44	`CGCTCCCGGGAA------------------------------CCGTGGGGCACCATCTTC`	`30 bp deleted → no frameshift`
38	`CGCTCCC-----------------------------------------------ATCTTC`	`47 bp deleted → frameshift`
36	`CGCTCCCGGGAAGTC------------------------------------------TTC`	`42 bp deleted → no frameshift`
36	`CGCTCC------------------------------------------------ATCTTC`	`48 bp deleted → no frameshift`
36	`CGC------------------------------------------------ACCATCTTC`	`48 bp deleted → no frameshift`
31	`CGCTCCCGGGAAGTC---------------------------------------------`	`45 bp deleted → no frameshift`
22	`CGCTC----------------------------------------------------TTC`	`52 bp deleted → frameshift`
20	`CGCT------------------------------------------------------TC`	`54 bp deleted → no frameshift`
19	`CGCTC-------------------------------------------------------`	`55 bp deleted → frameshift`

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