CRISPOR

DNA break repair outcome predictions

Guide: CGCGGGCTCAGGCAACCGTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTC`	(Wild-type sequence)
403	`AGGGCCGGCTCGCGGGCTCAGGCA-----------CCATCTTCTCTGGCGGTTCAGGCAG`	`11 bp deleted → frameshift`
341	`AGGGCCGGCTCGCGGGC-----------------ACCATCTTCTCTGGCGGTTCAGGCAG`	`17 bp deleted → frameshift`
303	`AGGGCCGGCTCGCGGGCTCAGGCAACC----------ATCTTCTCTGGCGGTTCAGGCAG`	`10 bp deleted → frameshift`
242	`AGGGCCGGCTCGCGGGCTCAGG----------GCACCATCTTCTCTGGCGGTTCAGGCAG`	`10 bp deleted → frameshift`
191	`AGGGCCGGCTCGCGGGCTCAGGCA-----------------------------------G`	`35 bp deleted → frameshift`
188	`AGGGC-----------------------------ACCATCTTCTCTGGCGGTTCAGGCAG`	`29 bp deleted → frameshift`
179	`AGGGCCGGCTCGCGGG----------------GCACCATCTTCTCTGGCGGTTCAGGCAG`	`16 bp deleted → frameshift`
172	`AGGGCCGGC-------------------------ACCATCTTCTCTGGCGGTTCAGGCAG`	`25 bp deleted → frameshift`
163	`AGGGCCGGCTCGCGGGCTCAGGC--------------------------GGTTCAGGCAG`	`26 bp deleted → frameshift`
162	`AGGGCCGGCTCGCGG------------------CACCATCTTCTCTGGCGGTTCAGGCAG`	`18 bp deleted → no frameshift`
149	`AGGGCCGGCTCGCGGGCTCAGGCA--------------TCTTCTCTGGCGGTTCAGGCAG`	`14 bp deleted → frameshift`
141	`AGGGCCGGCTCGCGGGCTCA---------------CCATCTTCTCTGGCGGTTCAGGCAG`	`15 bp deleted → no frameshift`
140	`AGGGCCGGCTCGC---------------------ACCATCTTCTCTGGCGGTTCAGGCAG`	`21 bp deleted → no frameshift`
136	`AGGGCCGGCTCGCGGGCTC--------------------------TGGCGGTTCAGGCAG`	`26 bp deleted → frameshift`
133	`AGGGCCGGCTCGCGGGCTCAGGCAACCG----------------------GTTCAGGCAG`	`22 bp deleted → frameshift`
132	`AGGGCCGGCTCGCGG------------------------------------TTCAGGCAG`	`36 bp deleted → no frameshift`
128	`AGGGCCGGCTCGCGGGCTCAGGCAACCGTG-----------------GCGGTTCAGGCAG`	`17 bp deleted → frameshift`
122	`AGGGCCGGCTCGCGGGCTCA------------------TCTTCTCTGGCGGTTCAGGCAG`	`18 bp deleted → no frameshift`
121	`AGGGCCGGCTCGCGGGC--------------------------------GGTTCAGGCAG`	`32 bp deleted → frameshift`
120	`AGGGCCGG------------------------GCACCATCTTCTCTGGCGGTTCAGGCAG`	`24 bp deleted → no frameshift`
105	`AGGGCCGGCTCGCGGGCTC---------------------TTCTCTGGCGGTTCAGGCAG`	`21 bp deleted → no frameshift`
98	`AGGG----------------------------GCACCATCTTCTCTGGCGGTTCAGGCAG`	`28 bp deleted → frameshift`
95	`AGGGCCGGCTCGCGGGCTCAGGCAACCGT-----------------------TCAGGCAG`	`23 bp deleted → frameshift`
95	`AGGGCCGGCTCGCGGGCT-----------------------TCTCTGGCGGTTCAGGCAG`	`23 bp deleted → frameshift`
94	`AGGGCCGGCTCGCGGGCTCAGG-----------------------------TTCAGGCAG`	`29 bp deleted → frameshift`
91	`AGGGCCGGCTC----------------------------------TGGCGGTTCAGGCAG`	`34 bp deleted → frameshift`
90	`AGGGCCGGCTCGCGGGCTC------------------------TCTGGCGGTTCAGGCAG`	`24 bp deleted → no frameshift`
89	`AGG------------------------------CACCATCTTCTCTGGCGGTTCAGGCAG`	`30 bp deleted → no frameshift`
84	`AGGGCC-------------------------------ATCTTCTCTGGCGGTTCAGGCAG`	`31 bp deleted → frameshift`
81	`AGGGCCGGC----------------------------------------GGTTCAGGCAG`	`40 bp deleted → frameshift`
77	`AGGGCCGGCTCGCGGGC-----------------------------------------AG`	`41 bp deleted → frameshift`
76	`AGGGCCGGCTCGCGG---------------------------------CGGTTCAGGCAG`	`33 bp deleted → no frameshift`
74	`AGGGCCGGCTCGCGGGCT----------------------------GGCGGTTCAGGCAG`	`28 bp deleted → frameshift`
71	`AGGGCCGGCTCGCGGGCTCAG---------------------------------------`	`39 bp deleted → no frameshift`
70	`AGGGCCGGCTC-----------------------------TTCTCTGGCGGTTCAGGCAG`	`29 bp deleted → frameshift`
69	`AGGGCCGG-------------------------------------------TTCAGGCAG`	`43 bp deleted → frameshift`
69	`AGGGCCGGCTCGCGGG-----------------------------------TTCAGGCAG`	`35 bp deleted → frameshift`
66	`AGGGC--------------------------------------------GGTTCAGGCAG`	`44 bp deleted → frameshift`
63	`AGGGCCGGCTCGCGGGCTCAGGCA-------------------------------GGCAG`	`31 bp deleted → frameshift`
63	`AGGGCCGGCT-------------------------------TCTCTGGCGGTTCAGGCAG`	`31 bp deleted → frameshift`
60	`AGGGCCGGCTC--------------------------------TCTGGCGGTTCAGGCAG`	`32 bp deleted → frameshift`
60	`AGGGCCGGCTCG--------------------------------------GTTCAGGCAG`	`38 bp deleted → frameshift`
51	`AGGGCCGGC-------------------------------------------------AG`	`49 bp deleted → frameshift`
49	`AGGGCCGGCT------------------------------------GGCGGTTCAGGCAG`	`36 bp deleted → no frameshift`
48	`AGGGCCGGCTCGCGG------------------------------------------CAG`	`42 bp deleted → no frameshift`
42	`AGGGC-----------------------------------------------------AG`	`53 bp deleted → frameshift`
42	`AGGGCCGGCTCGC---------------------------------------------AG`	`45 bp deleted → no frameshift`
42	`AGG---------------------------------------------CGGTTCAGGCAG`	`45 bp deleted → no frameshift`
38	`AGGG-----------------------------------------------TTCAGGCAG`	`47 bp deleted → frameshift`
36	`AGG------------------------------------------------TTCAGGCAG`	`48 bp deleted → no frameshift`
34	`AGGGCCGGCTC-------------------------------------------AGGCAG`	`43 bp deleted → frameshift`
33	`AGG------------------------------------------------------CAG`	`54 bp deleted → no frameshift`
16	`AG----------------------------------------------------------`	`58 bp deleted → frameshift`

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