CRISPOR

DNA break repair outcome predictions

Guide: CTCTGTGCCGCACCGCCTCT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ACCTTGTGATGCGAGCGCTCCTCTGTGCCGCACCGCCTCTGGGAGGAAGCCCCATTGCCCTCTTCTTTCTAAGCTGTCAT`	(Wild-type sequence)
298	`GCGAGCGCTCCTCTGTGCCGCACCGCC--------------CCATTGCCCTCTTCTTTCT`	`14 bp deleted → frameshift`
253	`GCGAGCGCTCCTCTGTGCCGCACCGCCTCT-----------------------TCTTTCT`	`23 bp deleted → frameshift`
199	`GCGAGCGCTCCTCTGTGCCGCACCGCC----------------------CTCTTCTTTCT`	`22 bp deleted → frameshift`
199	`GCGAGCGCTCCTCTGTGCC----------------------CCATTGCCCTCTTCTTTCT`	`22 bp deleted → frameshift`
188	`GCGAGCGCTCCTCTGTGCCGCACCGCC---------------CATTGCCCTCTTCTTTCT`	`15 bp deleted → no frameshift`
179	`GCGAGCGCTCCTCTGTGCCGCACCGCC----------------ATTGCCCTCTTCTTTCT`	`16 bp deleted → frameshift`
170	`GCGAGCGCTCCTCTGTGCCGCACC-----------------CCATTGCCCTCTTCTTTCT`	`17 bp deleted → frameshift`
162	`GCGAGCGCTCCTCTGTGCCGCACC------------------CATTGCCCTCTTCTTTCT`	`18 bp deleted → no frameshift`
156	`GCGAGCGCTCCTCTGTGCC------------------------------CTCTTCTTTCT`	`30 bp deleted → no frameshift`
154	`GCGAGCGCTCCTCTGTGCCGCACC-------------------ATTGCCCTCTTCTTTCT`	`19 bp deleted → frameshift`
154	`GCGAGCGCTCCTCTGTGCCGC-------------------CCCATTGCCCTCTTCTTTCT`	`19 bp deleted → frameshift`
126	`GCGAGCGCTCCTCTGTGCC-----------------------CATTGCCCTCTTCTTTCT`	`23 bp deleted → frameshift`
120	`GCGAGCGCTCCTCTGTGCC------------------------ATTGCCCTCTTCTTTCT`	`24 bp deleted → no frameshift`
114	`GCGAGCGCTCCTCTGTGCCGCACC-------------------------CTCTTCTTTCT`	`25 bp deleted → frameshift`
113	`GCGAGCGCTCCTCT---------------------------------------TCTTTCT`	`39 bp deleted → no frameshift`
111	`GCGAG------------------------------GAAGCCCCATTGCCCTCTTCTTTCT`	`30 bp deleted → no frameshift`
109	`GCGAGCGCTCCTCTGTGCCGCACCGCCTCT--------------------------TTCT`	`26 bp deleted → frameshift`
109	`GCGAGCGCTCCTCTGTGCCGCACC--------------------------TCTTCTTTCT`	`26 bp deleted → frameshift`
105	`GCGAGCGCTCCTCTGTGCCGCACCGCCTCT---------------------CTTCTTTCT`	`21 bp deleted → no frameshift`
103	`GCGAGCGCTCCTCTGTGCCGC---------------------------CCTCTTCTTTCT`	`27 bp deleted → no frameshift`
99	`GCGAGCGCTCCTCTGTGCCGCA----------------------TTGCCCTCTTCTTTCT`	`22 bp deleted → frameshift`
91	`GCGAGC----------------------------------CCCATTGCCCTCTTCTTTCT`	`34 bp deleted → frameshift`
89	`GCGAGCGCTCCTCTGTGCCGCACCGCCTCT------------------------------`	`30 bp deleted → no frameshift`
89	`GCGAGCGCTCC------------------------------CCATTGCCCTCTTCTTTCT`	`30 bp deleted → no frameshift`
86	`GCGAGCGCTCCTCTGTGCCGCACCGCCT-------------------------TCTTTCT`	`25 bp deleted → frameshift`
84	`GCGAGCGCTCCTCTGTGCC-------------------------------TCTTCTTTCT`	`31 bp deleted → frameshift`
84	`GCGAGCGCTCC-------------------------------CATTGCCCTCTTCTTTCT`	`31 bp deleted → frameshift`
80	`GCGAGCGCTCC--------------------------------ATTGCCCTCTTCTTTCT`	`32 bp deleted → frameshift`
80	`GCGAGCGC--------------------------------CCCATTGCCCTCTTCTTTCT`	`32 bp deleted → frameshift`
74	`GCGAGCGCTCCTCTGTGCCGCACCGCCT----------------------------TTCT`	`28 bp deleted → frameshift`
61	`GCGAGCGCTC------------------------------------------TTCTTTCT`	`42 bp deleted → no frameshift`
60	`GCGAGCGCTCCTCTGTGCCGCACCGCCT--------------------------------`	`32 bp deleted → frameshift`
60	`GCGAGCGCTCCTCTG--------------------------------CCCTCTTCTTTCT`	`32 bp deleted → frameshift`
60	`GCGAGCGCTCC--------------------------------------CTCTTCTTTCT`	`38 bp deleted → frameshift`
60	`GC--------------------------------------CCCATTGCCCTCTTCTTTCT`	`38 bp deleted → frameshift`
57	`GCGA---------------------------------AGCCCCATTGCCCTCTTCTTTCT`	`33 bp deleted → no frameshift`
54	`GCGAGCGC----------------------------------------CCTCTTCTTTCT`	`40 bp deleted → frameshift`
48	`GCGAGCGCTCCTCT------------------------------------------TTCT`	`42 bp deleted → no frameshift`
48	`GCGAGC------------------------------------------CCTCTTCTTTCT`	`42 bp deleted → no frameshift`
47	`GCGAGCGCTCCTCT-------------------------------------CTTCTTTCT`	`37 bp deleted → frameshift`
40	`GCGAGCGCTCCTCT----------------------------------------------`	`46 bp deleted → frameshift`
40	`GC----------------------------------------------CCTCTTCTTTCT`	`46 bp deleted → frameshift`
38	`GCGAGCGCTCCT-----------------------------------------TCTTTCT`	`41 bp deleted → frameshift`
33	`GCGAGCGCTCCT--------------------------------------------TTCT`	`44 bp deleted → frameshift`
33	`GCGAGCGCT--------------------------------------------TCTTTCT`	`44 bp deleted → frameshift`
31	`GCGAGCGCTC---------------------------------------------TTTCT`	`45 bp deleted → no frameshift`
28	`GCGAGCGCT-----------------------------------------------TTCT`	`47 bp deleted → frameshift`
27	`GCGAGCGCTCCT------------------------------------------------`	`48 bp deleted → no frameshift`
25	`GCGAGCGCTC-------------------------------------------------T`	`49 bp deleted → frameshift`
23	`GCGAGCGCT---------------------------------------------------`	`51 bp deleted → no frameshift`

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