CRISPOR

DNA break repair outcome predictions

Guide: TTGGCACTCTGGTTGGTAAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAGCAGCCCGCTGC`	(Wild-type sequence)
335	`GGGGGTCAGCTTGGCACTCTGGTTGGT--------CTCTAATTTGTCCGAAGCCGTGAGC`	`8 bp deleted → frameshift`
282	`GGGGGTCAGCTTGGCACTCTGGTTGG-------GTCTCTAATTTGTCCGAAGCCGTGAGC`	`7 bp deleted → frameshift`
274	`GGGGGTCAGCTTGGCACTCTGGT------------CTCTAATTTGTCCGAAGCCGTGAGC`	`12 bp deleted → no frameshift`
271	`GGGGGTCAGCTTGGCACTCTGGTTGGTAAG--GGTCTCTAATTTGTCCGAAGCCGTGAGC`	`2 bp deleted → frameshift`
232	`GGGGGTCAGCTTGGCACTCT-------------------AATTTGTCCGAAGCCGTGAGC`	`19 bp deleted → frameshift`
230	`GGGGGTCAGCTTGGCACTCTGG-----------GTCTCTAATTTGTCCGAAGCCGTGAGC`	`11 bp deleted → frameshift`
211	`GGGGGTC-----------------------------TCTAATTTGTCCGAAGCCGTGAGC`	`29 bp deleted → frameshift`
170	`GGGGGTCAGCTTGGCACTCT-----------------CTAATTTGTCCGAAGCCGTGAGC`	`17 bp deleted → frameshift`
164	`GGGGGTCAGCTTGGCACTCTGGTTGGTAA------------TTTGTCCGAAGCCGTGAGC`	`12 bp deleted → no frameshift`
154	`GGGGGTCAGCTTGG-------------------GTCTCTAATTTGTCCGAAGCCGTGAGC`	`19 bp deleted → frameshift`
147	`GGGGGTCAGCTTGGCACTCTGGTTG--------------------TCCGAAGCCGTGAGC`	`20 bp deleted → frameshift`
147	`GGGGGTCAGCTTGG--------------------TCTCTAATTTGTCCGAAGCCGTGAGC`	`20 bp deleted → frameshift`
133	`GGGG------------------------------TCTCTAATTTGTCCGAAGCCGTGAGC`	`30 bp deleted → no frameshift`
133	`GGGGGTCAGCTTGGCACTCTGGTTGGTAAG----------------------CCGTGAGC`	`22 bp deleted → frameshift`
127	`GGG-------------------------------TCTCTAATTTGTCCGAAGCCGTGAGC`	`31 bp deleted → frameshift`
116	`GGGGGTCAGCTTGGCACTCTGGTTGGT-------------------CCGAAGCCGTGAGC`	`19 bp deleted → frameshift`
105	`GGGGGTCAGCTTGGCACT---------------------AATTTGTCCGAAGCCGTGAGC`	`21 bp deleted → no frameshift`
98	`GGGGG----------------------------GTCTCTAATTTGTCCGAAGCCGTGAGC`	`28 bp deleted → frameshift`
95	`GGGGGTCAGCTTGGCACTCTGGT-----------------------CCGAAGCCGTGAGC`	`23 bp deleted → frameshift`
95	`GGGGGTCAG-----------------------GGTCTCTAATTTGTCCGAAGCCGTGAGC`	`23 bp deleted → frameshift`
90	`GGGGGTCAGCTTGGCACTCTG------------------------TCCGAAGCCGTGAGC`	`24 bp deleted → no frameshift`
81	`GGGGGTCAGCT--------------------------CTAATTTGTCCGAAGCCGTGAGC`	`26 bp deleted → frameshift`
80	`GGGGGTCAGCTTG--------------------------------TCCGAAGCCGTGAGC`	`32 bp deleted → frameshift`
80	`GG--------------------------------TCTCTAATTTGTCCGAAGCCGTGAGC`	`32 bp deleted → frameshift`
77	`GGGGGTCAGCTTGGCACTCTGGTT-------------------TGTCCGAAGCCGTGAGC`	`19 bp deleted → frameshift`
74	`GGGGGTCAGCTTGGCACTC----------------------------CGAAGCCGTGAGC`	`28 bp deleted → frameshift`
74	`GGGGGTCAGCT----------------------------AATTTGTCCGAAGCCGTGAGC`	`28 bp deleted → frameshift`
70	`GGGGGTCAGCTTGGCACTCTGGTTGGTAAG-----------------------------C`	`29 bp deleted → frameshift`
70	`GGGGGTCAGCTTGGCACTCTGGTTGGT-----------------------------GAGC`	`29 bp deleted → frameshift`
67	`GGGGGTC----------------------------------------CGAAGCCGTGAGC`	`40 bp deleted → frameshift`
63	`GGGGGTC-------------------------------TAATTTGTCCGAAGCCGTGAGC`	`31 bp deleted → frameshift`
60	`GGGGGTCAGCTTGGCACTCTGGTTG--------------------------------AGC`	`32 bp deleted → frameshift`
60	`GGGGGTCAGCTTGGC--------------------------------------CGTGAGC`	`38 bp deleted → frameshift`
58	`GGGGGTCAGC-------------------------------------------CGTGAGC`	`43 bp deleted → frameshift`
57	`GGGGGTCAGCTTGGCACTCTGGT---------------------------------GAGC`	`33 bp deleted → no frameshift`
49	`GGGGGTCAGCTTGGCACTCTG------------------------------------AGC`	`36 bp deleted → no frameshift`
42	`GGGGGTCAGCTT-------------------------------TGTCCGAAGCCGTGAGC`	`31 bp deleted → frameshift`
42	`GGGGGTCAGCTTGGC---------------------------------------------`	`45 bp deleted → no frameshift`
41	`GGGGGTCAGC--------------------------------------------------`	`50 bp deleted → frameshift`
33	`GGGGGTCAGCTTG--------------------------------------------AGC`	`44 bp deleted → frameshift`
24	`GGGGGT--------------------------------------------------GAGC`	`50 bp deleted → frameshift`

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