CRISPOR

DNA break repair outcome predictions

Guide: GGCTGTCCGCGGAGGGGACT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTG`	(Wild-type sequence)
564	`TGGCCAGAGAGGCTGTCCGCGGAGGGG-------GTCCCGGGCCAAGCATGGCCGCTCCA`	`7 bp deleted → frameshift`
536	`TGGCCAGAGAGGCTGTCCGCGGAGGGG--------TCCCGGGCCAAGCATGGCCGCTCCA`	`8 bp deleted → frameshift`
444	`TGGCCAGAGAGGCTGTCCGCGGAGGGG------GGTCCCGGGCCAAGCATGGCCGCTCCA`	`6 bp deleted → no frameshift`
382	`TGGCCAGAGAGGCTGTCCGCGGAGGG---------TCCCGGGCCAAGCATGGCCGCTCCA`	`9 bp deleted → no frameshift`
311	`TGGCCAGAGAGGCTGTCCGCGGAGGGG-----GGGTCCCGGGCCAAGCATGGCCGCTCCA`	`5 bp deleted → frameshift`
283	`TGGCCAGAGAGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCCA`	`15 bp deleted → no frameshift`
269	`TGGCCAGAGAGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCCA`	`16 bp deleted → frameshift`
257	`TGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGCCGCTCCA`	`20 bp deleted → frameshift`
242	`TGGCCAGAGAGGCTGTCCGCGGAGG----------TCCCGGGCCAAGCATGGCCGCTCCA`	`10 bp deleted → frameshift`
242	`TGGCCAGAGAGGCTGTCCGCGG----------GGGTCCCGGGCCAAGCATGGCCGCTCCA`	`10 bp deleted → frameshift`
232	`TGGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCCA`	`19 bp deleted → frameshift`
230	`TGGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGGCCGCTCCA`	`11 bp deleted → frameshift`
219	`TGGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGCCGCTCCA`	`12 bp deleted → no frameshift`
210	`TGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGCCGCTCCA`	`21 bp deleted → no frameshift`
208	`TGGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGCCGCTCCA`	`13 bp deleted → frameshift`
198	`TGGCCAGAGAGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCCA`	`14 bp deleted → frameshift`
170	`TGGCCAGAGAGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCCA`	`17 bp deleted → frameshift`
147	`TGGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCCA`	`20 bp deleted → frameshift`
147	`TGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCATGGCCGCTCCA`	`20 bp deleted → frameshift`
140	`TGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGGCCGCTCCA`	`21 bp deleted → no frameshift`
133	`TGGCCAGAGAGGC------------------------------CAAGCATGGCCGCTCCA`	`30 bp deleted → no frameshift`
133	`TGGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGCCGCTCCA`	`22 bp deleted → frameshift`
132	`TGGCCAGAGAGGCTGTCCGC------------------------------------TCCA`	`36 bp deleted → no frameshift`
127	`TGGCCA---------------------------------------AGCATGGCCGCTCCA`	`39 bp deleted → no frameshift`
126	`TGGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGCCGCTCCA`	`23 bp deleted → frameshift`
126	`TGGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGCCGCTCCA`	`23 bp deleted → frameshift`
114	`TGGCCAGAGAGGCTGTCCGCGGAGGGG-------------------------CCGCTCCA`	`25 bp deleted → frameshift`
109	`TGGCCAGAGAGGCTGTCCGCGGAGGG--------------------------CCGCTCCA`	`26 bp deleted → frameshift`
109	`TGGCCAGAGAGGCTGTCC--------------------------AAGCATGGCCGCTCCA`	`26 bp deleted → frameshift`
103	`TGGCCAGAGAGGCTGTCCGCGGAGG---------------------------CCGCTCCA`	`27 bp deleted → no frameshift`
98	`TGGCCAGAGAGGCTGTCCGC----------------------------ATGGCCGCTCCA`	`28 bp deleted → frameshift`
95	`TGGCCAGAGAGGCTGTCCGCGGAG-----------------------CATGGCCGCTCCA`	`23 bp deleted → frameshift`
94	`TGGCCAGAGAGG-----------------------------GCCAAGCATGGCCGCTCCA`	`29 bp deleted → frameshift`
94	`TGG-----------------------------GGGTCCCGGGCCAAGCATGGCCGCTCCA`	`29 bp deleted → frameshift`
89	`TGGCCAGAGAGGCTGTCCGCGG------------------------------CCGCTCCA`	`30 bp deleted → no frameshift`
89	`TGG------------------------------GGTCCCGGGCCAAGCATGGCCGCTCCA`	`30 bp deleted → no frameshift`
84	`TGG-------------------------------GTCCCGGGCCAAGCATGGCCGCTCCA`	`31 bp deleted → frameshift`
81	`TGGCCAGAGAGGC----------------------------------------CGCTCCA`	`40 bp deleted → frameshift`
80	`TGG--------------------------------TCCCGGGCCAAGCATGGCCGCTCCA`	`32 bp deleted → frameshift`
77	`TGGCCAGAGAGGCTGTCCGCGGAGGGGACT---------------------------CCA`	`27 bp deleted → no frameshift`
77	`TGGCC-------------------------------------------------GCTCCA`	`49 bp deleted → frameshift`
76	`TGGCCAGAGAGGCTGTCCGC---------------------------------CGCTCCA`	`33 bp deleted → no frameshift`
76	`TGGCC---------------------------------CGGGCCAAGCATGGCCGCTCCA`	`33 bp deleted → no frameshift`
73	`TGGCCAGAGAGGCTGTCCGCG----------------------------------CTCCA`	`34 bp deleted → frameshift`
73	`TGGCC----------------------------------GGGCCAAGCATGGCCGCTCCA`	`34 bp deleted → frameshift`
69	`TGGCCAGAGAGGC-----------------------------------ATGGCCGCTCCA`	`35 bp deleted → frameshift`
64	`TGGCCAGAGAGGCTGTCC-----------------------------------------A`	`41 bp deleted → frameshift`
60	`TGG--------------------------------------GCCAAGCATGGCCGCTCCA`	`38 bp deleted → frameshift`
58	`TGGCCAGAGAGGCT-------------------------------------------CCA`	`43 bp deleted → frameshift`
49	`TGGCCAGAGAGGCTG------------------------------------GCCGCTCCA`	`36 bp deleted → no frameshift`
49	`TGGCCAGAGAG------------------------------------CATGGCCGCTCCA`	`36 bp deleted → no frameshift`
45	`TGGCCAGAG--------------------------------------CATGGCCGCTCCA`	`38 bp deleted → frameshift`
44	`TGGC--------------------------------------------ATGGCCGCTCCA`	`44 bp deleted → frameshift`
40	`TGGCCAG----------------------------------------CATGGCCGCTCCA`	`40 bp deleted → frameshift`
34	`TGGCCA-------------------------------------------TGGCCGCTCCA`	`43 bp deleted → frameshift`
33	`TGGCCA------------------------------------------------------`	`54 bp deleted → no frameshift`
29	`TGGC----------------------------------------------------TCCA`	`52 bp deleted → frameshift`

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