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Contents:

PCR primers for off-targets of AGGAGCTAAGGCGACAAGGC AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313738_F TCGTCGGCAGCGTCCGCATCACAAGGTGACCCTA 59.7 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313738_R GTCTCGTGGGCTCGGCTCCTAATCCCAGGCTCCCT 60.1 1.00
mm3_intron_Chat_chr14_32425712_F TCGTCGGCAGCGTCAACCCACCAAATGCAGGGAT 59.8 0.58
mm3_intron_Chat_chr14_32425712_R GTCTCGTGGGCTCGGATGCCACCACACTCTCACAG 59.9 0.58
mm3_intergenic_Rbbp8|Gm6277_chr18_11804964_F Primer3: not found at this Tm N.d. 0.50
mm3_intergenic_Rbbp8|Gm6277_chr18_11804964_R Primer3: not found at this Tm N.d. 0.50
mm4_intergenic_Pgrmc2|Gm25487_chr3_41178226_F Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_Pgrmc2|Gm25487_chr3_41178226_R Primer3: not found at this Tm N.d. 0.49
mm4_intron_Farsb_chr1_78454919_F TCGTCGGCAGCGTCCCAGGAAGAAGGGTAGTGCG 60.1 0.40
mm4_intron_Farsb_chr1_78454919_R GTCTCGTGGGCTCGGTCCTTGAAGTCAGGAAGCTGG 59.6 0.40
mm4_intergenic_Gm14120|Gm25866_chr2_148587773_F TCGTCGGCAGCGTCAGTAAATGTCCCTATCTCTGAGTC 57.0 0.40
mm4_intergenic_Gm14120|Gm25866_chr2_148587773_R GTCTCGTGGGCTCGGACTTGGCTGACCTGAAAGCA 59.8 0.40
mm4_intergenic_Nav3|E2f7_chr10_110739320_F TCGTCGGCAGCGTCATGGTGTCGTACAAAGCAGT 57.4 0.36
mm4_intergenic_Nav3|E2f7_chr10_110739320_R GTCTCGTGGGCTCGGCGTGTGGCAGAAAGACGAGA 60.3 0.36
mm4_intergenic_Ttll7|Gm23131_chr3_147391461_F TCGTCGGCAGCGTCTGTGGGAATCAGCAGGTAGC 59.7 0.36
mm4_intergenic_Ttll7|Gm23131_chr3_147391461_R GTCTCGTGGGCTCGGCCCGCTTGTCTCCTTGAGAA 59.6 0.36
mm4_intergenic_Ifit1|Slc16a12_chr19_34655678_F TCGTCGGCAGCGTCCGGAAGACAGGCAGGAAGAC 60.3 0.33
mm4_intergenic_Ifit1|Slc16a12_chr19_34655678_R GTCTCGTGGGCTCGGTTCCCATGGTTCTCACTGCC 59.9 0.33
mm3_intron_Ncam1_chr9_49729636_F Primer3: not found at this Tm N.d. 0.31
mm3_intron_Ncam1_chr9_49729636_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Tars|Npr3_chr15_11706729_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Tars|Npr3_chr15_11706729_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm24591|Gm26241_chr8_44449475_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm24591|Gm26241_chr8_44449475_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Ccdc92_chr5_124843763_F TCGTCGGCAGCGTCCTGATACTCTGGCCAGGCTG 59.8 0.25
mm4_intron_Ccdc92_chr5_124843763_R GTCTCGTGGGCTCGGGGTGTGTTACAGGGTGTAGCA 59.9 0.25
mm4_intron_Chst4_chr8_110035941_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Chst4_chr8_110035941_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm23022|Rab28_chr5_41324225_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm23022|Rab28_chr5_41324225_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Slc25a21_chr12_56965842_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Slc25a21_chr12_56965842_R Primer3: not found at this Tm N.d. 0.21
mm4_exon_Csn2_chr5_87698036_F TCGTCGGCAGCGTCGCTCACTGCTGCTACTGTGT 60.3 0.20
mm4_exon_Csn2_chr5_87698036_R GTCTCGTGGGCTCGGTTCACAGGACTTGACAGCCA 59.1 0.20
mm4_intergenic_Rbms3|Gm20396_chr9_117368371_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Rbms3|Gm20396_chr9_117368371_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Ppp4r1l-ps_chr2_173583235_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Ppp4r1l-ps_chr2_173583235_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gzmb|Atp12a_chr14_56351540_F TCGTCGGCAGCGTCATATGTGAGTATGTGTGCTTTCAC 57.3 0.19
mm4_intergenic_Gzmb|Atp12a_chr14_56351540_R GTCTCGTGGGCTCGGGCTCCTTGAGGCCAAGTATGA 59.7 0.19
mm4_intergenic_Gm11922|Map3k7_chr4_31630338_F TCGTCGGCAGCGTCTGGAAAAGCTCAGAAGGTTGGA 59.8 0.19
mm4_intergenic_Gm11922|Map3k7_chr4_31630338_R GTCTCGTGGGCTCGGAGGACAGAAATGACCTGCGT 59.3 0.19
mm4_intergenic_Nhlrc2|Adrb1_chr19_56697430_F TCGTCGGCAGCGTCACGTGTCTGCCTCTGAAGTG 59.9 0.19
mm4_intergenic_Nhlrc2|Adrb1_chr19_56697430_R GTCTCGTGGGCTCGGTGAGTAAAGCCACGCCTGTT 59.8 0.19
mm4_intron_9530036O11Rik_chr5_28577153_F TCGTCGGCAGCGTCCTGGAATTTTGGCCAGCACA 59.3 0.18
mm4_intron_9530036O11Rik_chr5_28577153_R GTCTCGTGGGCTCGGGATGTGCATGTGACCCTGGA 60.0 0.18
mm3_intergenic_Gm17566|Gm23153_chr1_190404616_F TCGTCGGCAGCGTCCCCTCTCTCAACCAAATTTTCCT 58.5 0.18
mm3_intergenic_Gm17566|Gm23153_chr1_190404616_R GTCTCGTGGGCTCGGGAGAGAGCTCACCTGTGTGC 60.3 0.18
mm4_intergenic_Zfp148|Slc12a8_chr16_33505231_F TCGTCGGCAGCGTCAGGACAATGGAAGCTGGTGA 58.9 0.17
mm4_intergenic_Zfp148|Slc12a8_chr16_33505231_R GTCTCGTGGGCTCGGACCTGCATCAGTGTGTGAGA 58.9 0.17
mm4_intron_Cnr2_chr4_135910866_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Cnr2_chr4_135910866_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Rapgef4_chr2_72216886_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Rapgef4_chr2_72216886_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Mir5098|Rest_chr5_77274834_F TCGTCGGCAGCGTCTGCCAGTAATTGAGAGGAATCGT 59.8 0.16
mm4_intergenic_Mir5098|Rest_chr5_77274834_R GTCTCGTGGGCTCGGCTAGCACGAACGACCCTGTC 60.4 0.16
mm4_intron_Ky_chr9_102539704_F TCGTCGGCAGCGTCGCATGAGGGATGATGGCCTT 60.1 0.16
mm4_intron_Ky_chr9_102539704_R GTCTCGTGGGCTCGGAATGGGCAAGAGGTACCTGC 60.0 0.16
mm4_intron_Eps8_chr6_137621301_F TCGTCGGCAGCGTCTGGGCTAAGGAGAAGCAGTG 59.3 0.15
mm4_intron_Eps8_chr6_137621301_R GTCTCGTGGGCTCGGGAAGCCAACACAGCCTGGAA 60.8 0.15
mm4_intron_Ryr3_chr2_112806025_F TCGTCGGCAGCGTCATTACCACCACAGTCCTGGC 59.6 0.15
mm4_intron_Ryr3_chr2_112806025_R GTCTCGTGGGCTCGGGCATCCATCTGAGTGCACCT 60.1 0.15
mm4_intergenic_Gm13010|1700013G24Rik_chr4_137449002_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm13010|1700013G24Rik_chr4_137449002_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm23342|Myt1l_chr12_29225939_F TCGTCGGCAGCGTCATTTTCCCCTGGAGTGTGCC 60.2 0.15
mm4_intergenic_Gm23342|Myt1l_chr12_29225939_R GTCTCGTGGGCTCGGTTGAGAGGAGCAGAGGGACC 60.6 0.15
mm4_intron_St8sia5_chr18_77234593_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_St8sia5_chr18_77234593_R Primer3: not found at this Tm N.d. 0.14
mm3_intron_Pla2r1_chr2_60495901_F Primer3: not found at this Tm N.d. 0.13
mm3_intron_Pla2r1_chr2_60495901_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Foxl1|5033426O07Rik_chr8_121179831_F TCGTCGGCAGCGTCAACCAGCAAACACTGAGGCA 60.3 0.13
mm4_intergenic_Foxl1|5033426O07Rik_chr8_121179831_R GTCTCGTGGGCTCGGTCAGAGAGAGCTAGCTGCCT 59.7 0.13
mm4_intron_Mllt3_chr4_87991138_F TCGTCGGCAGCGTCATGAACACCAGGTCCCGTTC 59.9 0.13
mm4_intron_Mllt3_chr4_87991138_R GTCTCGTGGGCTCGGAGGCTGAGTTATGCCAGAGC 59.8 0.13
mm4_intergenic_Cecr5|Cecr2_chr6_120587195_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Cecr5|Cecr2_chr6_120587195_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_AI593442|Gm22242_chr9_52731303_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_AI593442|Gm22242_chr9_52731303_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Pcdhb20|Pcdhb21_chr18_37510483_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Pcdhb20|Pcdhb21_chr18_37510483_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm14602|Dcaf12l1_chrX_44708713_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm14602|Dcaf12l1_chrX_44708713_R Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Eda|Gm14832_chrX_100060861_F Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Eda|Gm14832_chrX_100060861_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Ldlrap1|Tmem57_chr4_134799039_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Ldlrap1|Tmem57_chr4_134799039_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_4932435O22Rik|Gm11689_chr11_114624916_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_4932435O22Rik|Gm11689_chr11_114624916_R Primer3: not found at this Tm N.d. 0.09
mm4_exon_Chpf_chr1_75474704_F TCGTCGGCAGCGTCTCTCAGCTTCACCTCCTCCA 59.8 0.09
mm4_exon_Chpf_chr1_75474704_R GTCTCGTGGGCTCGGGCTGTGGCCTCTGTTTATGC 59.5 0.09
mm4_intergenic_Oxnad1|Msmb_chr14_32122165_F TCGTCGGCAGCGTCTCACTATGGACGCCTCAGGA 60.0 0.09
mm4_intergenic_Oxnad1|Msmb_chr14_32122165_R GTCTCGTGGGCTCGGTCTGATTCCAAACCCAGCCC 59.9 0.09
mm4_intergenic_Fbxo15|Gm25453_chr18_85660817_F TCGTCGGCAGCGTCTGCTGTCAGTGTGGAGTTAGG 59.6 0.08
mm4_intergenic_Fbxo15|Gm25453_chr18_85660817_R GTCTCGTGGGCTCGGACAAGTGTCTGCACCTAGGC 59.9 0.08
mm3_exon_Chd3_chr11_69346808_F TCGTCGGCAGCGTCGTGACAGGTTCAGGTAGGCC 60.0 0.08
mm3_exon_Chd3_chr11_69346808_R GTCTCGTGGGCTCGGCCGGCCCTTCTACTCACTTG 60.1 0.08
mm4_intergenic_Rtkn2|Gm16212/Rtkn2_chr10_68018380_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Rtkn2|Gm16212/Rtkn2_chr10_68018380_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_AU019990_chr2_132604896_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_AU019990_chr2_132604896_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Cacna2d3|Selk_chr14_29874227_F TCGTCGGCAGCGTCTAACGCAGTCCGAGAACCTG 59.7 0.08
mm4_intergenic_Cacna2d3|Selk_chr14_29874227_R GTCTCGTGGGCTCGGCCATGCAGAGGAGGCAGAAA 60.0 0.08
mm4_intron_Ebf3_chr7_137307913_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Ebf3_chr7_137307913_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Camk1|Tada3_chr6_113362328_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Camk1|Tada3_chr6_113362328_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Gpr125_chr5_50016629_F TCGTCGGCAGCGTCTGAGTGGAGTCATAGCTTGAGG 59.2 0.06
mm4_intron_Gpr125_chr5_50016629_R GTCTCGTGGGCTCGGAGGGCAGAGAGGAGTGAAGT 59.8 0.06
mm3_intron_Ncor2_chr5_125090869_F Primer3: not found at this Tm N.d. 0.06
mm3_intron_Ncor2_chr5_125090869_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Rab36_chr10_75047688_F TCGTCGGCAGCGTCGATTGGATGTCAGGGTCCTCC 59.8 0.06
mm4_intron_Rab36_chr10_75047688_R GTCTCGTGGGCTCGGAGGTTGATCATGTCTGCCACA 59.6 0.06
mm4_intron_St8sia5_chr18_77249454_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_St8sia5_chr18_77249454_R Primer3: not found at this Tm N.d. 0.06
mm2_intergenic_2410076I21Rik|Hcn4_chr9_58818450_F TCGTCGGCAGCGTCCATGCCCCAGTAGAATCCCC 59.8 0.06
mm2_intergenic_2410076I21Rik|Hcn4_chr9_58818450_R GTCTCGTGGGCTCGGATCCCCTCTTCCTAGCACCC 60.4 0.06
mm4_exon_Tifab_chr13_56175023_F TCGTCGGCAGCGTCGCCTTCTGCTCCCTTCTGAG 60.1 0.06
mm4_exon_Tifab_chr13_56175023_R GTCTCGTGGGCTCGGTTCCTGAACGGACAATGGGG 59.9 0.06
mm4_intergenic_Rhox9|Gm15474_chrX_37903491_F TCGTCGGCAGCGTCTAGTGGTTCTGGGTTGGCTG 59.6 0.06
mm4_intergenic_Rhox9|Gm15474_chrX_37903491_R GTCTCGTGGGCTCGGTCTGGTGGTGGTGAGAAGGA 60.1 0.06
mm4_intergenic_Rhox5|Rhox6_chrX_37825260_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rhox5|Rhox6_chrX_37825260_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12462512_F TCGTCGGCAGCGTCCGCTTCTGAGGGGAAAGACC 60.3 0.06
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12462512_R GTCTCGTGGGCTCGGTGCTGGTTCTGTGTTTGGGA 59.7 0.06
mm4_intergenic_Tex35|Rasal2_chr1_157125191_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Tex35|Rasal2_chr1_157125191_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Tsc1_chr2_28689518_F TCGTCGGCAGCGTCCCTGCCTGCAAGCTAAGACT 60.0 0.05
mm4_exon_Tsc1_chr2_28689518_R GTCTCGTGGGCTCGGGAGCCTGTCTCCTCATGCTC 59.8 0.05
mm4_intron_Gpr37l1_chr1_135166460_F TCGTCGGCAGCGTCTCCTGGTGTTTGCATCCACA 59.8 0.05
mm4_intron_Gpr37l1_chr1_135166460_R GTCTCGTGGGCTCGGTCTTTTACTGTGTCTCTTGCCA 57.5 0.05
mm4_intergenic_Pop4|Gm22638_chr7_38317269_F TCGTCGGCAGCGTCGGTTGAACTCAGATCCCGGT 59.3 0.05
mm4_intergenic_Pop4|Gm22638_chr7_38317269_R GTCTCGTGGGCTCGGTCAAGACTACTGTGTCGGGA 57.7 0.05
mm4_intergenic_Gm6605|Gm25286_chr7_38573365_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm6605|Gm25286_chr7_38573365_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm6605|Gm21276_chr7_38726152_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm6605|Gm21276_chr7_38726152_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm6605|Gm21142_chr7_38870399_F TCGTCGGCAGCGTCTGGACTCAGATCCCGGTTCT 59.9 0.05
mm4_intergenic_Gm6605|Gm21142_chr7_38870399_R GTCTCGTGGGCTCGGTCAAGACTAGTGTGTCGGGA 57.7 0.05
mm4_intergenic_Gm5114|AC109210.1_chr7_39413812_F TCGTCGGCAGCGTCTGGACTCAGATCCCGGTTCT 59.9 0.05
mm4_intergenic_Gm5114|AC109210.1_chr7_39413812_R GTCTCGTGGGCTCGGTCAAGACTACTGTGTCGGGA 57.7 0.05
mm4_intergenic_Gm25671|Gm16387_chr7_39225578_F TCGTCGGCAGCGTCGGTTGAACTCAGATCCCGGT 59.3 0.05
mm4_intergenic_Gm25671|Gm16387_chr7_39225578_R GTCTCGTGGGCTCGGTCAAGACTACTGTGTCGGGA 57.7 0.05
mm4_intergenic_Gm23307|Gm5114_chr7_39368792_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm23307|Gm5114_chr7_39368792_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_4930433I11Rik|Gm4884_chr7_41032045_F TCGTCGGCAGCGTCTCAAGACTACTGTGTCGGGA 57.7 0.05
mm4_intergenic_4930433I11Rik|Gm4884_chr7_41032045_R GTCTCGTGGGCTCGGTGGACTCAGATCCCGGTTCT 59.9 0.05
mm4_intergenic_Gm9964|Nf1_chr11_79306198_F TCGTCGGCAGCGTCGATTCTCCCGGAAGCAGGAG 59.8 0.05
mm4_intergenic_Gm9964|Nf1_chr11_79306198_R GTCTCGTGGGCTCGGTCTACTGCTGGCTTGTGCAA 59.8 0.05
mm4_intergenic_Gm22223|Gm22715_chr8_101676920_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm22223|Gm22715_chr8_101676920_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Nat10_chr2_103748194_F TCGTCGGCAGCGTCAACAGACCCCTCCTCCTCTC 59.9 0.05
mm4_exon_Nat10_chr2_103748194_R GTCTCGTGGGCTCGGCTCGACATCCTGCCCATCTC 59.9 0.05
mm4_intergenic_Gm14314|Gm14292_chr2_179077458_F TCGTCGGCAGCGTCAGGGTTGCCAAACATCCTGT 59.8 0.05
mm4_intergenic_Gm14314|Gm14292_chr2_179077458_R GTCTCGTGGGCTCGGGTGTATGGAGATGCCCAGCT 59.5 0.05
mm4_intergenic_Gm25215|Epc2_chr2_49433967_F TCGTCGGCAGCGTCGCCTGCTATTCTAGTCAGGGC 60.2 0.04
mm4_intergenic_Gm25215|Epc2_chr2_49433967_R GTCTCGTGGGCTCGGTAGGCCCAGCTTGGTACAGA 60.2 0.04
mm4_intergenic_Crtc1|Klhl26_chr8_70444002_F TCGTCGGCAGCGTCGAAGCCACACAGAGCACACT 60.5 0.04
mm4_intergenic_Crtc1|Klhl26_chr8_70444002_R GTCTCGTGGGCTCGGCCCTTCACTCTAGCAGGCTG 59.8 0.04
mm4_intergenic_Mtss1|Gm5045_chr15_59174216_F TCGTCGGCAGCGTCGCCTAGTTCCGTTTGAGCCT 60.0 0.04
mm4_intergenic_Mtss1|Gm5045_chr15_59174216_R GTCTCGTGGGCTCGGCCAAGTCAAGAGGGGCTCTG 60.0 0.04
mm4_intron_Celsr3_chr9_108847907_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Celsr3_chr9_108847907_R Primer3: not found at this Tm N.d. 0.04
mm3_exon_Hlx_chr1_184732397_F TCGTCGGCAGCGTCCCCCTGGCTTGGCTTGAAA 60.5 0.04
mm3_exon_Hlx_chr1_184732397_R GTCTCGTGGGCTCGGGCGGAGTAGCAGAGCGAG 59.9 0.04
mm4_intergenic_Olfr1249|Olfr1250_chr2_89649937_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Olfr1249|Olfr1250_chr2_89649937_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Hopx|Gm15831_chr5_77116535_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Hopx|Gm15831_chr5_77116535_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ggta1_chr2_35415963_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ggta1_chr2_35415963_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Fcrl1_chr3_87377761_F TCGTCGGCAGCGTCAGCTCTTGAAACCTTTCCCC 57.7 0.04
mm4_intron_Fcrl1_chr3_87377761_R GTCTCGTGGGCTCGGTGAACACTTGCCTGGGTCAA 59.7 0.04
mm4_intergenic_Slc26a4|Bcap29_chr12_31575785_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Slc26a4|Bcap29_chr12_31575785_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm26653|Gm26105_chr6_136178848_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26653|Gm26105_chr6_136178848_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Plekha6_chr1_133276462_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Plekha6_chr1_133276462_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm6605|Gm5591_chr7_38452826_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm6605|Gm5591_chr7_38452826_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm22273|Gm24079_chr5_62388192_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm22273|Gm24079_chr5_62388192_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Cc2d2a_chr5_43670485_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Cc2d2a_chr5_43670485_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Sbno1_chr5_124377503_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Sbno1_chr5_124377503_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Crhr2_chr6_55126738_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Crhr2_chr6_55126738_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_G630016G05Rik|Gm13991_chr2_116443405_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_G630016G05Rik|Gm13991_chr2_116443405_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ntm_chr9_29818439_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ntm_chr9_29818439_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Cuedc1_chr11_88118821_F TCGTCGGCAGCGTCGCTAGGCCTCTGCTTCTGAG 59.8 0.03
mm4_intron_Cuedc1_chr11_88118821_R GTCTCGTGGGCTCGGGCAGGGACCAGAGGACAAAG 60.3 0.03
mm4_intergenic_Gm5454|n-R5s56_chr13_103365464_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm5454|n-R5s56_chr13_103365464_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cnih3|Gm16547_chr1_181381374_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cnih3|Gm16547_chr1_181381374_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Icmt|Icmt/Rnf207_chr4_152302730_F TCGTCGGCAGCGTCGGCTTATGTGGCCCTGAGG 60.1 0.03
mm4_intergenic_Icmt|Icmt/Rnf207_chr4_152302730_R GTCTCGTGGGCTCGGCTCCATCCGCCCTAATGTCC 59.9 0.03
mm4_intergenic_Sema7a|Gm17322_chr9_57965570_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Sema7a|Gm17322_chr9_57965570_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Cryzl1_chr16_91697659_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Cryzl1_chr16_91697659_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Lyrm4_chr13_36027681_F TCGTCGGCAGCGTCTCCATCCACAATCCCGAGTC 59.1 0.02
mm4_intron_Lyrm4_chr13_36027681_R GTCTCGTGGGCTCGGTGTGGTGGCTTTGGGTTCC 60.4 0.02
mm3_intergenic_Jazf1|Gm25818_chr6_53238931_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Jazf1|Gm25818_chr6_53238931_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dip2b_chr15_100192048_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dip2b_chr15_100192048_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11650|Gm23120_chr11_107814822_F TCGTCGGCAGCGTCGGGAGGCTAGGGAGACAGAA 60.0 0.02
mm4_intergenic_Gm11650|Gm23120_chr11_107814822_R GTCTCGTGGGCTCGGTGTCCCAGGATTAGGGCTCT 59.6 0.02
mm4_intron_Htr3b_chr9_48954321_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Htr3b_chr9_48954321_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ppp6r2_chr15_89260326_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ppp6r2_chr15_89260326_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Emx1|Sfxn5_chr6_85205224_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Emx1|Sfxn5_chr6_85205224_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Asxl3_chr18_22373368_F TCGTCGGCAGCGTCTGATGCCAGTTCAGCTCAGT 59.3 0.02
mm4_intron_Asxl3_chr18_22373368_R GTCTCGTGGGCTCGGTGGGCAGAGCAACAGTAATCA 59.6 0.02
mm4_intergenic_Atp1a1|Mab21l3_chr3_101722024_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Atp1a1|Mab21l3_chr3_101722024_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Pdzd2|Gm24135_chr15_12467363_F TCGTCGGCAGCGTCTTAGACCCGACTCCCCTGTC 60.3 0.02
mm4_intergenic_Pdzd2|Gm24135_chr15_12467363_R GTCTCGTGGGCTCGGGAGCCAGTTCACCTGACCAA 59.8 0.02
mm4_intron_Mtss1_chr15_59022025_F TCGTCGGCAGCGTCAGCAAATGCCACAAACGCTT 59.8 0.02
mm4_intron_Mtss1_chr15_59022025_R GTCTCGTGGGCTCGGTGAGCTGTGCATGTACCCAG 60.0 0.02
mm4_intergenic_Lrpap1|Adra2c_chr5_35214148_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Lrpap1|Adra2c_chr5_35214148_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Unc13a_chr8_71633961_F TCGTCGGCAGCGTCTGGGGTAATTCTGAACGGGG 59.3 0.02
mm4_intron_Unc13a_chr8_71633961_R GTCTCGTGGGCTCGGGTCCCTCTGAGCTCTCTCCA 60.0 0.02
mm4_intron_Igsf21_chr4_140189466_F TCGTCGGCAGCGTCCAGAGGAAGAGCTGTGTGCA 59.9 0.02
mm4_intron_Igsf21_chr4_140189466_R GTCTCGTGGGCTCGGGCACCAGCAGACTCAGTTCT 59.9 0.02
mm4_intergenic_Gm23056|Thsd7b_chr1_129146989_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23056|Thsd7b_chr1_129146989_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gap43_chr16_42340526_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Gap43_chr16_42340526_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hoxa10_chr6_52236736_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hoxa10_chr6_52236736_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tspan18_chr2_93245494_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tspan18_chr2_93245494_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_3830408C21Rik|Apoo-ps_chr13_107157244_F TCGTCGGCAGCGTCAGGAGGCTTGGGTCTTCCTA 59.5 0.01
mm4_intergenic_3830408C21Rik|Apoo-ps_chr13_107157244_R GTCTCGTGGGCTCGGCCTTGTCTGCTGACTCTGCA 59.9 0.01
mm4_intergenic_Gm26831|Gm8321_chr1_119071378_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26831|Gm8321_chr1_119071378_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3l_chr10_34058180_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3l_chr10_34058180_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Anp32a_chr9_62374746_F TCGTCGGCAGCGTCGGAGTATGATGAATATGCCCAGC 59.0 0.01
mm4_exon_Anp32a_chr9_62374746_R GTCTCGTGGGCTCGGCAGAGGTGGGACTGCACAG 60.0 0.01
mm4_intergenic_Gm22603|Gria1_chr11_56836457_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22603|Gria1_chr11_56836457_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tbpl2_chr2_24088688_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tbpl2_chr2_24088688_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Map4k4_chr1_39995559_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Map4k4_chr1_39995559_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ppp1r42|Gm15818_chr1_9986880_F TCGTCGGCAGCGTCGTGGTGAGGAAGGCCAATGA 59.9 0.01
mm4_intergenic_Ppp1r42|Gm15818_chr1_9986880_R GTCTCGTGGGCTCGGGGTGGGTGTTGACACTGACT 59.8 0.01
mm4_intron_Schip1_chr3_68607417_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Schip1_chr3_68607417_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Calcoco1|Hoxc13_chr15_102798619_F TCGTCGGCAGCGTCTGTCTGCGTTGTGCTAAGCT 60.2 0.01
mm3_intergenic_Calcoco1|Hoxc13_chr15_102798619_R GTCTCGTGGGCTCGGAGGGCTGCCTGTCAGAAAAG 60.2 0.01
mm4_intron_Pde4d_chr13_109946664_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pde4d_chr13_109946664_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Robo1_chr16_72750994_F TCGTCGGCAGCGTCTGGTGGTTTGAAGTTGGGGA 59.3 0.00
mm3_intron_Robo1_chr16_72750994_R GTCTCGTGGGCTCGGACTGCATCTCCCACAACCTT 59.2 0.00
mm4_intergenic_Bhlhe23/Gm14343|Gm14340_chr2_180785729_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Bhlhe23/Gm14343|Gm14340_chr2_180785729_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rybp|Gm23234_chr6_100407104_F TCGTCGGCAGCGTCCGAAGTGCCCATATGCCAC 58.9 0.00
mm4_intergenic_Rybp|Gm23234_chr6_100407104_R GTCTCGTGGGCTCGGGTTCAAAACCCCTAATTCACCTT 57.1 0.00
mm4_intergenic_Gm10543|Gm25578_chr1_124209147_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10543|Gm25578_chr1_124209147_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cdh5|Bean1_chr8_104170326_F TCGTCGGCAGCGTCGTCCCTTCTGCTGCCTCTAC 59.8 0.00
mm4_intergenic_Cdh5|Bean1_chr8_104170326_R GTCTCGTGGGCTCGGGACAGACAGACACGTGCAGA 59.9 0.00
mm4_exon_Zbtb21_chr16_97951853_F TCGTCGGCAGCGTCTACCTCAGTCACAGGGGAGG 59.9 0.00
mm4_exon_Zbtb21_chr16_97951853_R GTCTCGTGGGCTCGGGATGACAGGCCTCAAGTGCT 60.0 0.00
mm4_intron_Baiap2_chr11_119969641_F TCGTCGGCAGCGTCGGAGCTTGGCTTCTCACACA 60.2 0.00
mm4_intron_Baiap2_chr11_119969641_R GTCTCGTGGGCTCGGGGAAGTGACCATGTCCCCAC 60.3 0.00
mm4_intron_Anks1b_chr10_90886518_F TCGTCGGCAGCGTCAGAACATGCGGAGAAGTGCA 59.9 0.00
mm4_intron_Anks1b_chr10_90886518_R GTCTCGTGGGCTCGGAGAACGATGCCTTTCACGTCT 59.9 0.00
mm4_intergenic_Gm5272|1700008P02Rik_chr3_6559595_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5272|1700008P02Rik_chr3_6559595_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sp7_chr15_102362766_F TCGTCGGCAGCGTCCACCCAAGAGAAACCCACCA 59.8 0.00
mm4_intron_Sp7_chr15_102362766_R GTCTCGTGGGCTCGGGAGCTGAGCAGGGCCATG 60.5 0.00
mm4_intergenic_Cd72|Sit1_chr4_43455041_F TCGTCGGCAGCGTCCCCAGGACATCACCCCAATC 60.1 0.00
mm4_intergenic_Cd72|Sit1_chr4_43455041_R GTCTCGTGGGCTCGGTGCAGAATGGGGCTCATGAA 59.6 0.00
mm4_intron_Fbxl4_chr4_22410254_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fbxl4_chr4_22410254_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rph3a|Ptpn11_chr5_121089863_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rph3a|Ptpn11_chr5_121089863_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rsu1_chr2_13164860_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rsu1_chr2_13164860_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ofcc1_chr13_40286480_F TCGTCGGCAGCGTCGATCAGGTGGGGAACAACAGT 59.9 0.00
mm4_intron_Ofcc1_chr13_40286480_R GTCTCGTGGGCTCGGTGGAGAGGTTGTTATCAGATCAGT 58.9 0.00
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402891_F TCGTCGGCAGCGTCTTGCTGCTAATGGCCCTGAT 59.7 0.00
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402891_R GTCTCGTGGGCTCGGGCAGGCCAGGTTAGGTACAG 60.1 0.00
mm4_intron_Gm14809_chrX_99416998_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14809_chrX_99416998_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14809_chrX_99416861_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14809_chrX_99416861_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rgmb_chr17_15821769_F TCGTCGGCAGCGTCAGTCACTGTACTTTCCCCGA 57.9 0.00
mm4_intron_Rgmb_chr17_15821769_R GTCTCGTGGGCTCGGAGTTGGATCTTGGTCAGGTGA 58.6 0.00
mm4_intergenic_Serpina3n|Gm24334_chr12_104415832_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Serpina3n|Gm24334_chr12_104415832_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nrp1_chr8_128421857_F TCGTCGGCAGCGTCCAGGAGCTGAGACGAAGGTC 59.8 0.00
mm4_intron_Nrp1_chr8_128421857_R GTCTCGTGGGCTCGGATCAGGGTGGTCTGAGGTGA 59.8 0.00
mm4_intron_Ndc80_chr17_71500515_F TCGTCGGCAGCGTCACCATTTGTAATCCCAAGATGCTG 59.5 0.00
mm4_intron_Ndc80_chr17_71500515_R GTCTCGTGGGCTCGGCACAGTACAGCACTCCCCAG 60.0 0.00
mm4_intron_Igf2bp1_chr11_96004700_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Igf2bp1_chr11_96004700_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm973_chr1_59608302_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm973_chr1_59608302_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Thoc2|Gm5135_chrX_41921094_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Thoc2|Gm5135_chrX_41921094_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Hmgcl|Gale_chr4_135962841_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Hmgcl|Gale_chr4_135962841_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16397|Gm10344_chrX_134733602_F TCGTCGGCAGCGTCCCTTCTCCCTCACACAGCAG 60.0 0.00
mm4_intergenic_Gm16397|Gm10344_chrX_134733602_R GTCTCGTGGGCTCGGGGCTCTGGGAAGGCATCTAC 59.8 0.00
mm4_intergenic_Gm13063/Epb4.1|Epb4.1_chr4_132055369_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm13063/Epb4.1|Epb4.1_chr4_132055369_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10071|CLRN1-AS1_chr3_58992719_F TCGTCGGCAGCGTCGCACTGATGTTCAAGGTGGC 59.7 0.00
mm4_intergenic_Gm10071|CLRN1-AS1_chr3_58992719_R GTCTCGTGGGCTCGGTGAACATGAAGGCCAGGTGG 60.2 0.00
mm4_intergenic_Cyb5b|Rps18-ps3_chr8_107214159_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cyb5b|Rps18-ps3_chr8_107214159_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Actr8|Il17rb_chr14_29993379_F TCGTCGGCAGCGTCCCAGCTGTGGCATCTTCTCT 59.7 0.00
mm4_intergenic_Actr8|Il17rb_chr14_29993379_R GTCTCGTGGGCTCGGTGCTCCCTGACGTGTGTATG 59.7 0.00
mm4_exon_Trerf1_chr17_47341114_F TCGTCGGCAGCGTCGTGCACTTTCTTGGCAGGTG 59.9 0.00
mm4_exon_Trerf1_chr17_47341114_R GTCTCGTGGGCTCGGGTCAATGATTTCCGCCAGGC 59.8 0.00
mm3_intron_D930028M14Rik_chr7_25154882_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_D930028M14Rik_chr7_25154882_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313738 CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTG
CCTTGTCGCCTTAGCTCCT
CTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAG
mm3_intron_Chat_chr14_32425712 AACCCACCAAATGCAGGGATGGGGTGAGGAGAAAAGGAGACAAGGCTGGAAAATGCTTATGGAGGTAGGCTGGCCCAACC
CAGAGAGATGGCTAACTTGCTTGTATATAGTCACTGTGAGAGTGTGGTGGCAT
mm4_intron_Farsb_chr1_78454919 CCAGGAAGAAGGGTAGTGCGCGCCACCTACAGGAAAAGATGGGAATTCACACTGACTCAAGAACTGGCAAACTCAGGAGT
AAAGGAGACAAAGCCGG
CCAGCTTCCTGACTTCAAGGA
mm4_intergenic_Gm14120|Gm25866_chr2_148587773 AGTAAATGTCCCTATCTCTGAGTCCCTGACTTGTTGCCTTAGTTTCTCTCCTGCTTTCAGGTCAGCCAAGT
mm4_intergenic_Nav3|E2f7_chr10_110739320 ATGGTGTCGTACAAAGCAGTAATGTACATGTTTTTAAAAGTCAGCATCTTTCTTCTTTGAAATGAAGTTTAATCCCAGCC
TTGTCTCCTTTACCCCT
GCTACAATCTCGTCTTTCTGCCACACG
mm4_intergenic_Ttll7|Gm23131_chr3_147391461 TGTGGGAATCAGCAGGTAGCAATGCAACTGTAAGCTCCTGGATATCAGGGAGCAACTGAGAGCTATGGAGACAAGGCAGG
TAAAGGTGATCACAAAGATTCTGGGAAGACATCTTCTCAAGGAGACAAGCGGG
mm4_intergenic_Ifit1|Slc16a12_chr19_34655678 CGGAAGACAGGCAGGAAGACTAGGTGGAGAAAGAATTATTGGAGACTGAAGGAGAAAAGGGAACAAGGCAGGGAGGTGTG
GCAGTGAGAACCATGGGAA
mm4_intron_Ccdc92_chr5_124843763 CTGATACTCTGGCCAGGCTGCCCCTCCCCAGCCTTGTCCCCTCAGGTCCCCAGGGTGCTTTCCTGATCATCACCACAATA
TCCTTTGCCCCTCTTCTATCTGCTACACCCTGTAACACACC
mm4_exon_Csn2_chr5_87698036 GCTCACTGCTGCTACTGTGTGTAGCTAAGCAGACTTTTCTCTATATCTTCTTTACACACCTCTCTTGCAAGAGCAAGGGC
CACAAGGCAGG
CGAGGATGAAGACCTTCATGGCTGTCAAGTCCTGTGAA
mm4_intergenic_Gzmb|Atp12a_chr14_56351540 ATATGTGAGTATGTGTGCTTTCACATATACAATGCTAAATCACTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCATACTTGGCCTCAAGGAGC
mm4_intergenic_Gm11922|Map3k7_chr4_31630338 TGGAAAAGCTCAGAAGGTTGGACATTCTAGAAAGACATACCCAAATTCCGGCCTTGTGGCCTTAGCCCTGATCTTGAAAC
GCAGGTCATTTCTGTCCT
mm4_intergenic_Nhlrc2|Adrb1_chr19_56697430 ACGTGTCTGCCTCTGAAGTGCAGCAAAGGATGCAGCTAAGGAGACAAAGCCGGTCGTGGGGATCCACCAACAGGCGTGGC
TTTACTCA
mm4_intron_9530036O11Rik_chr5_28577153 CTGGAATTTTGGCCAGCACATTTGTATATTATCTTACACAGACATCCTTGTATGGATCCATCTTGGATAAGGTTGGGAGC
TTTGGAGACAAGGCAGG
ATAAAGCAAACTATTTCCAGGGTCACATGCACATC
mm3_intergenic_Gm17566|Gm23153_chr1_190404616 CCCTCTCTCAACCAAATTTTCCTCTAAGGAGCAAAGGCTACAAAGCGGGTCATACAGGTGAGCTCTCAGAGCGGGGCACA
CAGGTGAGCTCTCTC
mm4_intergenic_Zfp148|Slc12a8_chr16_33505231 AGGACAATGGAAGCTGGTGATGGAAATGTTTTCCCCGCAGGAGCTACAGAGGCAAGGCTGGTGGGCACATCCTCCATGGA
TAAGTGTTAGCATGTTGACTGTTGTGATCTCACACACTGATGCAGGT
mm4_intergenic_Mir5098|Rest_chr5_77274834 TGCCAGTAATTGAGAGGAATCGTTTTGCTCTTAAGTTGCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNN
NNNNNNNNNNNNNNNNNNNNNCAGGACAGGGTCGTTCGTGCTAG
mm4_intron_Ky_chr9_102539704 GCATGAGGGATGATGGCCTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTGATGGGATAAGGGGACAAGGCAGGGGG
GTCCACGAGGGGGCAGTCAGGAGCAGGTACCTCTTGCCCATT
mm4_intron_Eps8_chr6_137621301 TGGGCTAAGGAGAAGCAGTGAGGAGCAGAGGTGACAAGCCAGGCAGTGTACCCCACTGTTCCAGGCTGTGTTGGCTTC
mm4_intron_Ryr3_chr2_112806025 ATTACCACCACAGTCCTGGCAGGTGTGGATGGTACCAGATCCTTCATTCAGGCCAGATGATAAAACTCGAGCTAAGGGGA
CAAGGAAGG
TTATTGAATCTACCAGAGAAAGGATTAGAGGTGCACTCAGATGGATGC
mm4_intergenic_Gm23342|Myt1l_chr12_29225939 ATTTTCCCCTGGAGTGTGCCTCTCTCCCCAGAGAGCTCCCTGCCTTGTCCCCTTGCCTCCCCCACCAGGACACTCCAGGA
GGTCCCTCTGCTCCTCTCAA
mm4_intergenic_Foxl1|5033426O07Rik_chr8_121179831 AACCAGCAAACACTGAGGCAGATGAACCTCCAAGAAGAAACTCACCCTAGCACCAAGGCAGGGGCAAGTGGGAGCTGAGG
GCACAAGGCAGG
CAGAAGGCAGCTAGCTCTCTCTGA
mm4_intron_Mllt3_chr4_87991138 ATGAACACCAGGTCCCGTTCCAGTTAAGCTAATGAAGCCCATTAAATAGAGAGCCCTGGCTTGTCGCCTGAGCTCGACCC
CAGGGCTCTGGCATAACTCAGCCT
mm4_exon_Chpf_chr1_75474704 TCTCAGCTTCACCTCCTCCAACAATCCATCTACAGCCCAGCTCCCAGGACAGGAGCCCAGGAGGCAAGGCTGGCGTCAGG
CATAAACAGAGGCCACAGC
mm4_intergenic_Oxnad1|Msmb_chr14_32122165 TCACTATGGACGCCTCAGGAATGACACCGTGCTTGTCACCTTAGGTCCTCAAATACTAAATGATGTCATAAACTTCAATA
ACAAAATGAGCCTTCAAAACTCCAGGTGGGGCTGGGTTTGGAATCAGA
mm4_intergenic_Fbxo15|Gm25453_chr18_85660817 TGCTGTCAGTGTGGAGTTAGGACTTGGCTAGCTCCCAGCCTCGATGCTTTAGCTCCTTCAGTTGGTCACATTGCTTAGCC
TAGGTGCAGACACTTGT
mm3_exon_Chd3_chr11_69346808 GTGACAGGTTCAGGTAGGCCGCCCGCCGCAGCTGCTCCTCGATCACCAGCGCCTGCTCCAGGAGCTAAGGGCGCAAGGCA
GG
GGCAAAGACAAGTGAGTAGAAGGGCCGG
mm4_intergenic_Cacna2d3|Selk_chr14_29874227 TAACGCAGTCCGAGAACCTGAAAAGGTCTTGAGGAGCCAAGATGAAAAGGCGGGGTGACAGGGCCCCTGCTTTCTGCCTC
CTCTGCATGG
mm4_intron_Gpr125_chr5_50016629 TGAGTGGAGTCATAGCTTGAGGATTGTCTCTACAGGAGTTATGGCGACAGAGCTGGCATGGTAATGAGTCTGTTAGGACA
AGAGAGACATCCAAAGCACACTGACAGAGCACTTCACTCCTCTCTGCCCT
mm4_intron_Rab36_chr10_75047688 GATTGGATGTCAGGGTCCTCCCTACATATTGGCAACCCATTGTAAGACTATTCAAGCAGCTTCCCACCTTGTCGCCTCCC
CTCCT
CCATCTTGTGGCAGACATGATCAACCT
mm2_intergenic_2410076I21Rik|Hcn4_chr9_58818450 CATGCCCCAGTAGAATCCCCAGGGAACAATGGGTGCCCAGGATGGGAGAAAAGCTGGTAGATGAGGAGCTAAGGCTCCAA
GGCTGG
CCTGTGGGTGCTAGGAAGAGGGGAT
mm4_exon_Tifab_chr13_56175023 GCCTTCTGCTCCCTTCTGAGAAAATTCTCCCATCCTTGCCTACTTAGCTCCTTAACAATCCACCATCACCCCCATTGTCC
GTTCAGGAA
mm4_intergenic_Rhox9|Gm15474_chrX_37903491 TAGTGGTTCTGGGTTGGCTGTGTCCACTCCAACCCTCCTTGTAGCCCTAGCACCTCTGCAACATATGTGAGCAACTTGTA
AACTTGAACTTACTTGAACTTGGGTGCTTCCTTCTCACCACCACCAGA
mm4_intergenic_Rpl30-ps11|Atp6ap2_chrX_12462512 CGCTTCTGAGGGGAAAGACCTGGCTGAGGGGCAGAAGCCTTCAAGAAGGACATAGGATTTTCCAGGAACTGAGGTGACAG
GGCTGG
CAGCCATGTGGGGTATTGTCCCAAACACAGAACCAGCA
mm4_exon_Tsc1_chr2_28689518 CCTGCCTGCAAGCTAAGACTGTGCTTTAGGAGCCAGGGCAACATGGCAGGGAGCATGAGGAGACAGGCTC
mm4_intron_Gpr37l1_chr1_135166460 TCCTGGTGTTTGCATCCACAGGGAATAAACACAGACTCAACCTGGGGTGTTAGTAAGAAATTGCTAGGATGTTGGGATAC
CAAAGGGTTAAAAACAGGAGCCATGGGTACAAGGCAGGTGGCAAGAGACACAGTAAAAGA
mm4_intergenic_Pop4|Gm22638_chr7_38317269 GGTTGAACTCAGATCCCGGTTCTCTCCTTTTCATCCCTGCTTTGTAGCCTTTGATCCTTGGACCAGCTTGCTCCTGCATC
CTTACACTAGAGTGTGATAGCACAGTAAATTTCCCGACACAGTAGTCTTGA
mm4_intergenic_Gm6605|Gm21142_chr7_38870399 TGGACTCAGATCCCGGTTCTCTCCTTTTCATCCCTGCTTTGTAGCCTTTGATCCTTGGACCAGCTTGCTCCTGCATCCTT
ACACTAGAGTGTGAGAGCACAGTAAATTTCCCGACACACTAGTCTTGA
mm4_intergenic_Gm5114|AC109210.1_chr7_39413812 TGGACTCAGATCCCGGTTCTCTCCTTTTCATCCCTGCTTTGTAGCCTTTGATCCTTGGACCAGCTTGCTCCTGCATCCTT
ACACTAGAGTGTGATAGCACAGTAAATTTCCCGACACAGTAGTCTTGA
mm4_intergenic_Gm25671|Gm16387_chr7_39225578 GGTTGAACTCAGATCCCGGTTCTCTCCTTTTCATCCCTGCTTTGTAGCCTTTGATCCTTGGACCAGCTTGCTCCTGCATC
CTTACACTAGAGTGTGATAGCACAGTAAATTTCCCGACACAGTAGTCTTGA
mm4_intergenic_4930433I11Rik|Gm4884_chr7_41032045 TCAAGACTACTGTGTCGGGAAATTTACTGTGCTATCACACTCTAGTGTAAGGATGCAGGAGCAAGCTGGTCCAAGGATCA
AAGGCTACAAAGCAGG
GATGAAAAGGAGAGAACCGGGATCTGAGTCCA
mm4_intergenic_Gm9964|Nf1_chr11_79306198 GATTCTCCCGGAAGCAGGAGTTCCTGTGGAGGTGGGGCTGGTTCTGCTGAGAGAGAAGGAAGGTTTCTAGAAGAGGTGGA
GGGAGCTGAGGCCACACGGCTGGATAGGAGCTCACTTGCACAAGCCAGCAGTAGA
mm4_exon_Nat10_chr2_103748194 AACAGACCCCTCCTCCTCTCACCCAGCCTCACCGGGGCCTGAGGAGGTAAGGCTTCAATGCTGGCCACGTGGGAGGAGAT
GGGCAGGATGTCGAG
mm4_intergenic_Gm14314|Gm14292_chr2_179077458 AGGGTTGCCAAACATCCTGTTAGGATCTGAATTAATTTGGTAATAATGAGATCACATTGAAATCTCTATGTGATGTTTCA
GATAACAGAGCCCAGGACCTAAGGTAACATGGCAGGTTTGAGCTGGGCATCTCCATACAC
mm4_intergenic_Gm25215|Epc2_chr2_49433967 GCCTGCTATTCTAGTCAGGGCTAGGTTAGGAGCCATGGTGAAAAGGCCGGCTGGGCAGTGATGATGTTGTCATGTCGTGT
TAGACAATGACTGTTTGATAAGTGATCATGTTATATCTGTACCAAGCTGGGCCTA
mm4_intergenic_Crtc1|Klhl26_chr8_70444002 GAAGCCACACAGAGCACACTGCAAGTGCTAAGGCCCCAAGGCAGGCTGCAGCCTGCTAGAGTGAAGGG
mm4_intergenic_Mtss1|Gm5045_chr15_59174216 GCCTAGTTCCGTTTGAGCCTTGCAGCTGGACATCCATTCTTGCTTGTTGAGGACAGATGCTAAGGGGAAAAGGCAGGAGC
AGAGCCCCTCTTGACTTGG
mm3_exon_Hlx_chr1_184732397 CCCCTGGCTTGGCTTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGATGGAGGCGATCGCCAGGCGCTGGGGCG
ACAAGGCGAG
CGCGGCCTCGCTCTGCTACTCCGC
mm4_intron_Fcrl1_chr3_87377761 AGCTCTTGAAACCTTTCCCCCAGATGTCAATCAAAGCTGGGAACATTTCTATCATATCAAAGATTGCTTCAAGCTCCCAA
TCTTGTCCCCTGAGCTCCT
CTCGCATTCTTGACCCAGGCAAGTGTTCA
mm4_intron_Cuedc1_chr11_88118821 GCTAGGCCTCTGCTTCTGAGGAATTCTATTCTCACTCTCAAGAGCCCCACGGAGCCTCCAGGGTCCAGCCCTGTCCCCTT
TCCTCCT
TGCCTTTGTCCTCTGGTCCCTGC
mm4_intergenic_Icmt|Icmt/Rnf207_chr4_152302730 GGCTTATGTGGCCCTGAGGGGAGGAGGCAAGGGGCCAAGGCTGGCAAGTATGCTCAGTGGGTGGACATTAGGGCGGATGG
AG
mm4_intron_Lyrm4_chr13_36027681 TCCATCCACAATCCCGAGTCTCAGTTTTGAATGGCAAATGGACACAGCTGGCCAGATGTGCTCTGCATGTCTTTCTTTTC
CAGGATGATTTCCCCCCTTGTCTCCTCATCTCCTTGCCTGGGGAACCCAAAGCCACCACA
mm4_intergenic_Gm11650|Gm23120_chr11_107814822 GGGAGGCTAGGGAGACAGAAACCCATCAGCTAAGATGGATGAGCTAAGGGGAAAAGCCAGGAGAGCCCTAATCCTGGGAC
A
mm4_intron_Asxl3_chr18_22373368 TGATGCCAGTTCAGCTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNAAATATGTGTGATTACTGTTGCTCTGCCCA
mm4_intergenic_Pdzd2|Gm24135_chr15_12467363 TTAGACCCGACTCCCCTGTCATCCCCTGACCCACGCCATCCCTGTCGCCAGAGCTCCTCCCTGGGCTGGAAGACCTCAGG
TTTGGTCAGGTGAACTGGCTC
mm4_intron_Mtss1_chr15_59022025 AGCAAATGCCACAAACGCTTTGCCCCTCCTCAGCTCCTCTGCACCTACAGCTTCTCATTCCCAAAGGCTTTGGGAATATT
GCACCCCCCCTTGTCACTTCAGCTCCTTCTTATACATGGCTGGGTACATGCACAGCTCA
mm4_intron_Unc13a_chr8_71633961 TGGGGTAATTCTGAACGGGGGTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTAGGACCTTGGACCCCTGCCCT
GTGGCCTCAGCTGCT
GCATGGAGAGAGCTCAGAGGGAC
mm4_intron_Igsf21_chr4_140189466 CAGAGGAAGAGCTGTGTGCAGGGGCGTCCTCAGGGGTGTCTCCTGGGACAGCAACTGTGAGCCATGGAGAAGGAGCAAAG
GGGACAGGGTTGG
TCCCCGGGTACTATGAAACAGAACTGAGTCTGCTGGTGC
mm4_intergenic_3830408C21Rik|Apoo-ps_chr13_107157244 AGGAGGCTTGGGTCTTCCTAAGGAGGCAAGGAGAGAAGGCCGGGAGAGAGGACTGCAGAGTCAGCAGACAAGG
mm4_exon_Anp32a_chr9_62374746 GGAGTATGATGAATATGCCCAGCTAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGA
GTGGAGAGGAGGAGGTAAGGAGACGGGGCTGGGCACCTGTGCAGTCCCACCTCTG
mm4_intergenic_Ppp1r42|Gm15818_chr1_9986880 GTGGTGAGGAAGGCCAATGAGAGGCATTTGGAAGCAGCCTTCCAGGCTTCTTGCCTTACCTCCTCTCTCCCTATTGCATC
TCTTTCCTCAGTCAGTGTCAACACCCACC
mm3_intergenic_Calcoco1|Hoxc13_chr15_102798619 TGTCTGCGTTGTGCTAAGCTCTCCTCAGCCTTGTAGCCTCAGCACCTGACAAAGACCCTGACTTATCTGTAAGGCCTGGA
GGAGTTACCTTTTCTGACAGGCAGCCCT
mm3_intron_Robo1_chr16_72750994 TGGTGGTTTGAAGTTGGGGATAGGAGCTAAGGAGAAAAGGGAGGGTTTGATTCTCATTTTGGAGGGTGAAAAAGAAGCTG
AAAGGTTGTGGGAGATGCAGT
mm4_intergenic_Rybp|Gm23234_chr6_100407104 CGAAGTGCCCATATGCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAAGGTGAATTAGGGGTTTTGAAC
mm4_intergenic_Cdh5|Bean1_chr8_104170326 GTCCCTTCTGCTGCCTCTACCCTGCCTTCTGCCCTTAGCTCTTGCCTCTGTCCAACCTTTCTGGCTGTCCATCTCTGACA
TATGGTTGGTTCACCCTTCTCTGCACGTGTCTGTCTGTC
mm4_exon_Zbtb21_chr16_97951853 TACCTCAGTCACAGGGGAGGCTTCCTCCCTGTCTGTTGACTGGGAAGCACTAAAGGACCTGAGGCGATGAGGCTGGAGCA
CTTGAGGCCTGTCATC
mm4_intron_Baiap2_chr11_119969641 GGAGCTTGGCTTCTCACACAAAGGGTCAAGTTTGCTTTACCAAAGGAGCTTAGGCGCCATGGATGGGAGTGGGGACATGG
TCACTTCC
mm4_intron_Anks1b_chr10_90886518 AGAACATGCGGAGAAGTGCATACGGGAAGCAAGGAGCTAACGAGAGAAGGTTGGAGACGTGAAAGGCATCGTTCT
mm4_intron_Sp7_chr15_102362766 CACCCAAGAGAAACCCACCATGGCAAAGGGCCCCAGAAAACCCCCGGACCAGACCAGGCCACTCCTCCCTCCTTCTCCCC
TTAGCTGCT
GCCCCCTCCCCCTCTGCCCAGCATGGCCCTGCTCAGCTC
mm4_intergenic_Cd72|Sit1_chr4_43455041 CCCAGGACATCACCCCAATCACTACTCCAGGTTGCAGCTCCGTCCTCAACCATCAGATTTGGGGTTCCTACCATGGAGCC
TTAGCTCCT
CATTCCTGTGGGTTACCAGGAGAGAAGGTTCATGAGCCCCATTCTGCA
mm4_intron_Ofcc1_chr13_40286480 GATCAGGTGGGGAACAACAGTAGTGAGAACTAATGAGAATATCATGGTGGGGGGGGGGGGAGGGGGAGGGGCTAAGGCTA
CGAGGGAGG
GGAGCTTATGGTTTTTATTCTGTTGTGACTGATCTGATAACAACCTCTCCA
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402891 TTGCTGCTAATGGCCCTGATGTATTCTGTCTCTCTTTGTTGCCCCACACTCCAGCTATGGGGTTCCCCCCCTTCTCGTCT
TACCTCCT
CTTACCACTCTGTACCTAACCTGGCCTGC
mm4_intron_Rgmb_chr17_15821769 AGTCACTGTACTTTCCCCGAAGTACATTACTAATAAGCTACATCAATGCATCCAGCCACAACGCCTTAGCTCCTATAAGA
CCTAAGTACTTGGCTAAAAAAGTGATTCACCTGACCAAGATCCAACT
mm4_intron_Nrp1_chr8_128421857 CAGGAGCTGAGACGAAGGTCTGTCCTGCCTGGCAGCCTTAGCCCCTCTTCACCTTTCCCTCGGTCACCTCAGACCACCCT
GAT
mm4_intron_Ndc80_chr17_71500515 ACCATTTGTAATCCCAAGATGCTGCCTGCCTGCTTGCCTTAGCTGCTCCCCTGGGGAGTGCTGTACTGTG
mm4_intergenic_Gm16397|Gm10344_chrX_134733602 CCTTCTCCCTCACACAGCAGTTCCCCTTTACACAGCCCTCCCAAGTCCCCTTAGCTCCTGCCTCACCAAGGCTAACTGCC
CTGTGTGCCTGCCATGTGTCAGGAGTAGATGCCTTCCCAGAGCC
mm4_intergenic_Gm10071|CLRN1-AS1_chr3_58992719 GCACTGATGTTCAAGGTGGCAAGTATAACCCTGCCCTGCCTGGTCACCTGACCTCCTCACCTACTTAACCTCCATGCCCA
CCTGGCCTTCATGTTCA
mm4_intergenic_Actr8|Il17rb_chr14_29993379 CCAGCTGTGGCATCTTCTCTTTTTTTCTAGCAATAGTTAGGAGCTAAAGCGGTCAGGCTGGGTTCTGTGTACATCACACA
AGGTCCTCTTGGAGTTAAGCACTGTCTTTGTAACACATACACACGTCAGGGAGCA
mm4_exon_Trerf1_chr17_47341114 GTGCACTTTCTTGGCAGGTGAAGTCCAAGACAGTGGCTCAGTGCGTGGAGTACTACTACACTTGGAAGAAGATAATGCGA
CTAGGCCGG
AAACACCGGACACGCCTGGCGGAAATCATTGAC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.