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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313822_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313822_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intergenic_Acaa2|Gm24559_chr18_74901975_F | Primer3: not found at this Tm | N.d. | 0.85 |
| mm3_intergenic_Acaa2|Gm24559_chr18_74901975_R | Primer3: not found at this Tm | N.d. | 0.85 |
| mm4_intergenic_Gm24193|Gm20563_chr13_61964932_F | Primer3: not found at this Tm | N.d. | 0.70 |
| mm4_intergenic_Gm24193|Gm20563_chr13_61964932_R | Primer3: not found at this Tm | N.d. | 0.70 |
| mm3_intergenic_Scaf4|Hunk_chr16_90292829_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm3_intergenic_Scaf4|Hunk_chr16_90292829_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intron_Itgav_chr2_83777780_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intron_Itgav_chr2_83777780_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Diap3|Gm23278_chr14_87345277_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Diap3|Gm23278_chr14_87345277_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intron_Uty_chrY_1201505_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Uty_chrY_1201505_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Svopl_chr6_37996678_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Svopl_chr6_37996678_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Nfs1_chr2_156130140_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Nfs1_chr2_156130140_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Mgam_chr6_40634045_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Mgam_chr6_40634045_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Jmjd1c_chr10_67212430_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Jmjd1c_chr10_67212430_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Zfp825|Erap1_chr13_74561761_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Zfp825|Erap1_chr13_74561761_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Terf2|C630050I24Rik_chr8_107108245_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Terf2|C630050I24Rik_chr8_107108245_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Rpl39-ps|Atp5g2_chr15_102649571_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Rpl39-ps|Atp5g2_chr15_102649571_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Npas4|Slc29a2_chr19_5015143_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Npas4|Slc29a2_chr19_5015143_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Hmga2|Gm24298_chr10_120494671_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Hmga2|Gm24298_chr10_120494671_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm26099|Gm24809_chrX_37067035_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm26099|Gm24809_chrX_37067035_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111465443_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111465443_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm23708|G6pd2_chr5_60748206_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm23708|G6pd2_chr5_60748206_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm13787|1110051M20Rik_chr2_91370021_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm13787|1110051M20Rik_chr2_91370021_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Cnpy2|Cs_chr10_128332652_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Cnpy2|Cs_chr10_128332652_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_exon_Tmem209_chr6_30507158_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_exon_Tmem209_chr6_30507158_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intron_Cspp1_chr1_10114874_F | Primer3: not found at this Tm | N.d. | 0.54 |
| mm4_intron_Cspp1_chr1_10114874_R | Primer3: not found at this Tm | N.d. | 0.54 |
| mm4_intron_Tex2_chr11_106565957_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Tex2_chr11_106565957_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Myzap/Gcom1_chr9_71550963_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Myzap/Gcom1_chr9_71550963_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Kat8_chr7_127913915_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Kat8_chr7_127913915_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Heatr5b_chr17_78788475_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Heatr5b_chr17_78788475_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Ddx42_chr11_106234164_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Ddx42_chr11_106234164_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Zc3h12a|1700041M05Rik_chr4_125142023_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Zc3h12a|1700041M05Rik_chr4_125142023_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_exon_Rrm2b_chr15_37954535_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_exon_Rrm2b_chr15_37954535_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Gm25433|Senp6_chr9_80055960_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Gm25433|Senp6_chr9_80055960_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Arhgef33_chr17_80358447_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Arhgef33_chr17_80358447_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Stk35|Tgm3_chr2_129842979_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Stk35|Tgm3_chr2_129842979_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Cntn5|Gm25365_chr9_11936145_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Cntn5|Gm25365_chr9_11936145_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Amn1|2810474O19Rik_chr6_149195380_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Amn1|2810474O19Rik_chr6_149195380_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64607819_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64607819_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intron_Hoga1_chr19_42065487_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intron_Hoga1_chr19_42065487_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intron_4933416M07Rik_chr8_27145704_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intron_4933416M07Rik_chr8_27145704_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Gyg|Cpa3_chr3_20191589_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gyg|Cpa3_chr3_20191589_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Stk32c|U6_chr7_139191647_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Stk32c|U6_chr7_139191647_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Lasp1_chr11_97808239_F | TCGTCGGCAGCGTCGGGCTCACACAAACCCTGAT | 60.2 | 0.40 |
| mm4_intron_Lasp1_chr11_97808239_R | GTCTCGTGGGCTCGGCTAAAGCAGTCCAGCACCCA | 59.9 | 0.40 |
| mm4_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726019_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726019_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924063_F | TCGTCGGCAGCGTCAAGATGCAGGGCCAGGAATC | 60.1 | 0.37 |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924063_R | GTCTCGTGGGCTCGGGCATCGCCAGATCTCCATGA | 59.9 | 0.37 |
| mm4_intergenic_n-R5s157|Gm25423_chr7_116675080_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_n-R5s157|Gm25423_chr7_116675080_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_B3galnt1|Gm25621_chr3_69681680_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_B3galnt1|Gm25621_chr3_69681680_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Camsap2_chr1_136306036_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Camsap2_chr1_136306036_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Nlgn2|Tmem256_chr11_69837856_F | TCGTCGGCAGCGTCCACACCCCTCGCTTGCAC | 61.3 | 0.34 |
| mm4_intergenic_Nlgn2|Tmem256_chr11_69837856_R | GTCTCGTGGGCTCGGCCCTGCTCTAGTTCGCTACG | 59.9 | 0.34 |
| mm4_intergenic_Hmg20a|Gm26868_chr9_56547330_F | TCGTCGGCAGCGTCTGTCTGGGAACTGGCAAAGG | 60.1 | 0.34 |
| mm4_intergenic_Hmg20a|Gm26868_chr9_56547330_R | GTCTCGTGGGCTCGGCTCTTCCACCCAGCCATCTC | 59.8 | 0.34 |
| mm4_intron_Znrf3_chr11_5426874_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intron_Znrf3_chr11_5426874_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105438043_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105438043_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intron_Sept5_chr16_18625809_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Sept5_chr16_18625809_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Lypd6b_chr2_49866801_F | TCGTCGGCAGCGTCTAATCACATGGGCGCTGTGA | 59.7 | 0.32 |
| mm4_intron_Lypd6b_chr2_49866801_R | GTCTCGTGGGCTCGGGAGACTTGCAGAGCACAGCT | 60.3 | 0.32 |
| mm4_intron_Otc_chrX_10293740_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Otc_chrX_10293740_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Hs3st3b1|Cox10/9630013K17Rik_chr11_63953002_F | TCGTCGGCAGCGTCACAATTCTGAGGGAGCAGCC | 60.0 | 0.29 |
| mm4_intergenic_Hs3st3b1|Cox10/9630013K17Rik_chr11_63953002_R | GTCTCGTGGGCTCGGTCTGGTTCTGAGCCTGCTTG | 59.9 | 0.29 |
| mm4_intergenic_Gm23484|Otol1_chr3_70006790_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm23484|Otol1_chr3_70006790_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3_intron_Sorl1_chr9_41973182_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intron_Sorl1_chr9_41973182_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_exon_Map3k3_chr11_106123797_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Map3k3_chr11_106123797_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Tmem135_chr7_89347859_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Tmem135_chr7_89347859_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Zfp64_chr2_168911037_F | TCGTCGGCAGCGTCGGGGCAAGTAGCTGAAGATGA | 59.7 | 0.24 |
| mm4_intron_Zfp64_chr2_168911037_R | GTCTCGTGGGCTCGGGTACCTTAGGTGGAGTGCCG | 59.8 | 0.24 |
| mm4_intergenic_Cacng3|Rbbp6_chr7_122901764_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Cacng3|Rbbp6_chr7_122901764_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm13232|Gm13244_chr4_145745021_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm13232|Gm13244_chr4_145745021_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803442_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803442_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm25115|Diap3_chr14_86510617_F | TCGTCGGCAGCGTCGCGATGCCAGAAGAGCAAGA | 60.7 | 0.23 |
| mm4_intergenic_Gm25115|Diap3_chr14_86510617_R | GTCTCGTGGGCTCGGTGCATGATGGCGATGTTCCT | 60.1 | 0.23 |
| mm4_intron_Igf1r_chr7_68160317_F | TCGTCGGCAGCGTCTGCCCTTTAACCATGCCTGT | 59.8 | 0.23 |
| mm4_intron_Igf1r_chr7_68160317_R | GTCTCGTGGGCTCGGCTGCTCTCCATGAGTGCCAA | 60.0 | 0.23 |
| mm4_intron_Ip6k3_chr17_27157174_F | TCGTCGGCAGCGTCTCCCAGATTCCCCATGTCCT | 59.9 | 0.23 |
| mm4_intron_Ip6k3_chr17_27157174_R | GTCTCGTGGGCTCGGACATAGGACCAAGGGAGGCT | 59.9 | 0.23 |
| mm4_intergenic_Iscu|Tmem119_chr5_113781049_F | TCGTCGGCAGCGTCCCCAGCTGTCTTTGGAACCT | 59.8 | 0.23 |
| mm4_intergenic_Iscu|Tmem119_chr5_113781049_R | GTCTCGTGGGCTCGGGGTCCAGAAACCACTCTGCA | 59.8 | 0.23 |
| mm4_intergenic_RP24-86O15.2|Gm10698_chr9_33318595_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_RP24-86O15.2|Gm10698_chr9_33318595_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Tanc1_chr2_59630193_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Tanc1_chr2_59630193_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Tenm4_chr7_96444920_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Tenm4_chr7_96444920_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_exon_Nfatc1_chr18_80698419_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_exon_Nfatc1_chr18_80698419_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm2574|Gm24728_chr3_143031512_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm2574|Gm24728_chr3_143031512_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Ccdc122|Enox1_chr14_77122195_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Ccdc122|Enox1_chr14_77122195_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Zfr_chr15_12162709_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Zfr_chr15_12162709_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_exon_Tmem233_chr5_116040634_F | TCGTCGGCAGCGTCTCCCGACTAGTGGGTGTTCA | 60.1 | 0.19 |
| mm4_exon_Tmem233_chr5_116040634_R | GTCTCGTGGGCTCGGCTCATCTGCACACACTCCCA | 59.6 | 0.19 |
| mm4_intergenic_mmu-mir-6383|Tceal5_chrX_136190264_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_mmu-mir-6383|Tceal5_chrX_136190264_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Arhgap27_chr11_103342554_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Arhgap27_chr11_103342554_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Gab3_chrX_74970886_F | TCGTCGGCAGCGTCGTGTCTGCCTGACTCTGGAC | 60.0 | 0.18 |
| mm4_intron_Gab3_chrX_74970886_R | GTCTCGTGGGCTCGGTTGCTGTGACCCTGCAGAAT | 59.8 | 0.18 |
| mm4_intergenic_Gm14758|Gm14695_chrX_74778194_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm14758|Gm14695_chrX_74778194_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm14694|Gm6897_chrX_74854869_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm14694|Gm6897_chrX_74854869_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Col9a1_chr1_24179517_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Col9a1_chr1_24179517_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Olfr835|Olfr836_chr9_19076750_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Olfr835|Olfr836_chr9_19076750_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Dbn1_chr13_55482797_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Dbn1_chr13_55482797_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Tet3_chr6_83441473_F | TCGTCGGCAGCGTCGCGCCTTCCAGTGTTTTGTT | 59.8 | 0.16 |
| mm4_intron_Tet3_chr6_83441473_R | GTCTCGTGGGCTCGGGATGCAGTAGTGGAGGCCG | 60.2 | 0.16 |
| mm4_intron_Fam65a_chr8_105620729_F | TCGTCGGCAGCGTCATCCGTGTCCTCCAGAGTGT | 60.2 | 0.16 |
| mm4_intron_Fam65a_chr8_105620729_R | GTCTCGTGGGCTCGGCTGTCTAGCTCATGCCCAGG | 59.8 | 0.16 |
| mm4_intergenic_Gm25597|Gm26097_chr7_59796800_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm25597|Gm26097_chr7_59796800_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm22047|Gm25597_chr7_59794255_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm22047|Gm25597_chr7_59794255_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ppp2r5c_chr12_110490108_F | TCGTCGGCAGCGTCTTCAGGACCAAGGCCACATC | 59.9 | 0.15 |
| mm4_intron_Ppp2r5c_chr12_110490108_R | GTCTCGTGGGCTCGGTCTGTAATCCTGCAGAGGGC | 59.1 | 0.15 |
| mm4_intron_Camsap3_chr8_3590975_F | TCGTCGGCAGCGTCGGTAAGACCCAGTGTGGTCC | 59.6 | 0.15 |
| mm4_intron_Camsap3_chr8_3590975_R | GTCTCGTGGGCTCGGCCCCTTGGTGGTCTCAAGAC | 59.9 | 0.15 |
| mm4_intergenic_C330021F23Rik|Gm10186/C330021F23Rik_chr8_3583111_F | TCGTCGGCAGCGTCCCCCTTGGTGGTCTCAAGAC | 59.9 | 0.15 |
| mm4_intergenic_C330021F23Rik|Gm10186/C330021F23Rik_chr8_3583111_R | GTCTCGTGGGCTCGGGGTAAGACCCAGTGTGGTCC | 59.6 | 0.15 |
| mm3_intergenic_Gm6772|Gm25888_chr12_86805611_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Gm6772|Gm25888_chr12_86805611_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_4930407G08Rik|Slc1a7_chr4_107961964_F | TCGTCGGCAGCGTCAGGTGCAGGCATGAGTCTTC | 60.0 | 0.14 |
| mm4_intergenic_4930407G08Rik|Slc1a7_chr4_107961964_R | GTCTCGTGGGCTCGGAACCTCTGTAGAAGGGCCCT | 59.8 | 0.14 |
| mm4_intergenic_Gm11514|Gm11515_chr11_95196670_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm11514|Gm11515_chr11_95196670_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm8079|Otud4_chr8_79625027_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm8079|Otud4_chr8_79625027_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_128003406_F | TCGTCGGCAGCGTCAATGGTGGCTGGAACTCCTG | 59.9 | 0.14 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_128003406_R | GTCTCGTGGGCTCGGCTGAGGCACACAGTGGTCTC | 60.3 | 0.14 |
| mm4_intron_Arhgap44_chr11_65057189_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Arhgap44_chr11_65057189_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_6430706_F | TCGTCGGCAGCGTCCCCATACCCCTTTTGGTCCC | 60.0 | 0.13 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_6430706_R | GTCTCGTGGGCTCGGCTCCCAGTTCCATGCACAGA | 59.6 | 0.13 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823915_F | TCGTCGGCAGCGTCCACTACTGGGGCCACGATTG | 60.7 | 0.13 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823915_R | GTCTCGTGGGCTCGGTTACATACAGGGCGAGCCTG | 59.5 | 0.13 |
| mm4_intron_Cmip_chr8_117417549_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Cmip_chr8_117417549_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Olfr353_chr2_36890046_F | TCGTCGGCAGCGTCTCGCAGCTGCTACAACATCC | 60.7 | 0.12 |
| mm4_exon_Olfr353_chr2_36890046_R | GTCTCGTGGGCTCGGTTCTGGCGGGAGTTCAAAGG | 60.2 | 0.12 |
| mm3_intergenic_Gm8730|Cdh5_chr8_103672673_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Gm8730|Cdh5_chr8_103672673_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Fam163b_chr2_27118672_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Fam163b_chr2_27118672_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm11523|Cdk5rap3_chr11_96901092_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11523|Cdk5rap3_chr11_96901092_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Gm22527|Rnf144a_chr12_25805741_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Gm22527|Rnf144a_chr12_25805741_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Nbeal2|Ccdc12_chr9_110654209_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Nbeal2|Ccdc12_chr9_110654209_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Rph3a_chr5_120976168_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Rph3a_chr5_120976168_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Pdzd2_chr15_12436492_F | TCGTCGGCAGCGTCTCCCCTCTTCCCTCAAAGGT | 59.8 | 0.09 |
| mm4_intron_Pdzd2_chr15_12436492_R | GTCTCGTGGGCTCGGAGCACGGTTAAGCTTTGGGA | 59.8 | 0.09 |
| mm4_intergenic_Evc|Evc2_chr5_37337994_F | TCGTCGGCAGCGTCTCACAGAAGACCCTGTCCCT | 59.8 | 0.09 |
| mm4_intergenic_Evc|Evc2_chr5_37337994_R | GTCTCGTGGGCTCGGTAAGCTGGGGCAGAAAGCTC | 60.0 | 0.09 |
| mm4_intron_Aldh1a2_chr9_71236732_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Aldh1a2_chr9_71236732_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Nos2_chr11_78940287_F | TCGTCGGCAGCGTCCCAAACTGCCTCACCCTCAT | 59.9 | 0.09 |
| mm4_exon_Nos2_chr11_78940287_R | GTCTCGTGGGCTCGGTGGAGCACAGCCACATTGAT | 59.9 | 0.09 |
| mm4_intron_Sik1_chr17_31847486_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Sik1_chr17_31847486_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_1700029M20Rik|Ifnlr1_chr4_135666384_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_1700029M20Rik|Ifnlr1_chr4_135666384_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Uck2_chr1_167262026_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Uck2_chr1_167262026_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ccdc85c_chr12_108245827_F | TCGTCGGCAGCGTCGTGATTCGACCTCCCTGAGC | 60.1 | 0.08 |
| mm4_intron_Ccdc85c_chr12_108245827_R | GTCTCGTGGGCTCGGATCGCAATGAAGGACACCGT | 60.0 | 0.08 |
| mm4_intergenic_Gm12439|Gm12438_chr4_48720100_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm12439|Gm12438_chr4_48720100_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_G930045G22Rik_chr6_50871218_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_G930045G22Rik_chr6_50871218_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ass1_chr2_31509700_F | TCGTCGGCAGCGTCGCGTGTTCATGCATGTGTGT | 60.0 | 0.08 |
| mm4_intron_Ass1_chr2_31509700_R | GTCTCGTGGGCTCGGGGCCTGCAGTTGAAAACCTG | 59.9 | 0.08 |
| mm4_intergenic_Gm5506|Gm5839_chr18_48626189_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm5506|Gm5839_chr18_48626189_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Rreb1_chr13_37793156_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Rreb1_chr13_37793156_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm9522|Rims2_chr15_39197906_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm9522|Rims2_chr15_39197906_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Glp1r_chr17_30901944_F | TCGTCGGCAGCGTCGCTATGACCCAGTCCTGAGC | 59.8 | 0.06 |
| mm4_exon_Glp1r_chr17_30901944_R | GTCTCGTGGGCTCGGCTCCCATTTGTCACCGGGG | 60.3 | 0.06 |
| mm3_intergenic_Aldh1a3|Asb7_chr7_66626676_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Aldh1a3|Asb7_chr7_66626676_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116171770_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116171770_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Fgf9|Gm25614_chr14_58313302_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Fgf9|Gm25614_chr14_58313302_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138508596_F | TCGTCGGCAGCGTCTCGCCTTCTTGCTGTGACAT | 59.9 | 0.05 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138508596_R | GTCTCGTGGGCTCGGGGACTGTGGCTCCTCTGATG | 59.8 | 0.05 |
| mm4_exon_Klc3_chr7_19404089_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Klc3_chr7_19404089_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm5507|Redrum_chr18_54202223_F | TCGTCGGCAGCGTCTCCTGAGACAACAGAAGATGGT | 58.7 | 0.05 |
| mm3_intergenic_Gm5507|Redrum_chr18_54202223_R | GTCTCGTGGGCTCGGGTGCTTGCAAACCCATGGG | 60.0 | 0.05 |
| mm4_intron_Kat6b_chr14_21499595_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kat6b_chr14_21499595_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Il12rb1|Arrdc2_chr8_70828504_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Il12rb1|Arrdc2_chr8_70828504_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Abca3_chr17_24374148_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Abca3_chr17_24374148_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gpr56_chr8_95013743_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gpr56_chr8_95013743_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_exon_Trpc2_chr7_102077762_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_exon_Trpc2_chr7_102077762_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tmem232_chr17_65448525_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tmem232_chr17_65448525_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Csrp2bp_chr2_144403438_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Csrp2bp_chr2_144403438_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dok7_chr5_35079662_F | TCGTCGGCAGCGTCGGGACAGGCAACGGAAGG | 60.3 | 0.04 |
| mm4_exon_Dok7_chr5_35079662_R | GTCTCGTGGGCTCGGCCTTGAGTCCGCCACAGATT | 60.0 | 0.04 |
| mm4_exon_Col26a1_chr5_136743181_F | TCGTCGGCAGCGTCAGGACCTCCTCGCTTCATCT | 60.0 | 0.04 |
| mm4_exon_Col26a1_chr5_136743181_R | GTCTCGTGGGCTCGGGTTCCTTCTGCCCTCCTTCC | 60.0 | 0.04 |
| mm4_intron_Sned1_chr1_93241387_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sned1_chr1_93241387_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Folr4_chr9_14901002_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Folr4_chr9_14901002_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dbnl_chr11_5793633_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dbnl_chr11_5793633_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Efcab4b|Prmt8_chr6_127678157_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Efcab4b|Prmt8_chr6_127678157_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405107_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405107_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Lama3_chr18_12359152_F | TCGTCGGCAGCGTCCCACATCACCTTGGAGCTGT | 59.9 | 0.03 |
| mm4_intron_Lama3_chr18_12359152_R | GTCTCGTGGGCTCGGAGCTGCCACAAGAAAGTCCA | 59.8 | 0.03 |
| mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068369_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068369_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Atp2c1|Gm5621_chr9_105553787_F | TCGTCGGCAGCGTCCTTTGGTCACAGGGCCTAGG | 60.0 | 0.03 |
| mm4_intergenic_Atp2c1|Gm5621_chr9_105553787_R | GTCTCGTGGGCTCGGGCCCGGGTGCTAAACTCTAA | 59.7 | 0.03 |
| mm4_intergenic_Gm6027|Gm4746_chrX_90406658_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm6027|Gm4746_chrX_90406658_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111185518_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111185518_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Foxi3|Gm23485_chr6_71037233_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Foxi3|Gm23485_chr6_71037233_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Ppfia2_chr10_106762063_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Ppfia2_chr10_106762063_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911237_F | TCGTCGGCAGCGTCCGGGGTCAGTGGTTCACTC | 60.0 | 0.03 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911237_R | GTCTCGTGGGCTCGGGACAGCCCCGAGACACCAG | 62.3 | 0.03 |
| mm4_intron_Tdp1_chr12_99910040_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tdp1_chr12_99910040_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fzd10|Piwil1_chr5_128629017_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fzd10|Piwil1_chr5_128629017_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24614|Stmn2_chr3_8487756_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24614|Stmn2_chr3_8487756_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Celsr2_chr3_108397927_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Celsr2_chr3_108397927_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cux1_chr5_136305411_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cux1_chr5_136305411_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Evx1|Gm8129_chr6_52338901_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Evx1|Gm8129_chr6_52338901_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Dnal4|Nptxr/Npcd_chr15_79783792_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Dnal4|Nptxr/Npcd_chr15_79783792_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Atp8b1|Gm23016_chr18_64716286_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Atp8b1|Gm23016_chr18_64716286_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a30|Gm6425_chr19_8498410_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a30|Gm6425_chr19_8498410_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Ppfia2_chr10_106546247_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Ppfia2_chr10_106546247_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm22815|Cntn5_chr9_9403423_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm22815|Cntn5_chr9_9403423_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm11394|Gm11395_chr13_33736559_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm11394|Gm11395_chr13_33736559_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Fam173b|Tas2r119_chr15_31894007_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Fam173b|Tas2r119_chr15_31894007_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Ccl25|Cers4_chr8_4447090_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Ccl25|Cers4_chr8_4447090_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Obox2|RP24-105I10.3_chr7_15406874_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Obox2|RP24-105I10.3_chr7_15406874_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Obox1|RP23-163B8.6_chr7_15564846_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Obox1|RP23-163B8.6_chr7_15564846_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm24999|Gm25000_chr13_60312838_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm24999|Gm25000_chr13_60312838_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm23276|Dach1_chr14_97723585_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm23276|Dach1_chr14_97723585_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_4933433F19Rik|Dusp26_chr8_31047632_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_4933433F19Rik|Dusp26_chr8_31047632_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Lmx1a|Gm20711_chr1_168095477_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Lmx1a|Gm20711_chr1_168095477_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nfix|Dand5_chr8_84802088_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nfix|Dand5_chr8_84802088_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ezr_chr17_6741157_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ezr_chr17_6741157_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Kdr_chr5_75973347_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Kdr_chr5_75973347_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm20424|Lbx1_chr19_45201005_F | TCGTCGGCAGCGTCGGCATTTCAGACTGGGCTCT | 60.0 | 0.01 |
| mm4_intergenic_Gm20424|Lbx1_chr19_45201005_R | GTCTCGTGGGCTCGGTTTCCTCCTGCAAACCCCTT | 59.4 | 0.01 |
| mm4_intergenic_Scgb2b25-ps|Gm12757_chr7_33861516_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Scgb2b25-ps|Gm12757_chr7_33861516_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm1815|Zfp353-ps_chr8_41909137_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm1815|Zfp353-ps_chr8_41909137_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_D3Ertd751e|Gm25714_chr3_41770032_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_D3Ertd751e|Gm25714_chr3_41770032_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Naif1|Gm13412_chr2_32517278_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Naif1|Gm13412_chr2_32517278_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gkn3|Bmp10_chr6_87412077_F | TCGTCGGCAGCGTCTGGTGAGCAGGGAGACAAAG | 59.6 | 0.01 |
| mm4_intergenic_Gkn3|Bmp10_chr6_87412077_R | GTCTCGTGGGCTCGGCCCCTGATAATGGCTGGCTT | 59.8 | 0.01 |
| mm3_intron_Atf2_chr2_73819575_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Atf2_chr2_73819575_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22090|Gm9673_chrX_105020522_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22090|Gm9673_chrX_105020522_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Dgat2_chr7_99176680_F | TCGTCGGCAGCGTCAGGACCAAGCTCAGAGGGAT | 59.9 | 0.00 |
| mm4_intron_Dgat2_chr7_99176680_R | GTCTCGTGGGCTCGGCAGGGCCCATGACTTCCTTA | 59.0 | 0.00 |
| mm2_intergenic_Slc22a23|Pxdc1_chr13_34568901_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intergenic_Slc22a23|Pxdc1_chr13_34568901_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tsku_chr7_98355066_F | TCGTCGGCAGCGTCACAGCCCAGATGTTGCTCTC | 60.0 | 0.00 |
| mm4_intron_Tsku_chr7_98355066_R | GTCTCGTGGGCTCGGCAGGGGTTTGTTGGCTTGTG | 59.8 | 0.00 |
| mm4_intergenic_Padi1|4930515B02Rik_chr4_140871158_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Padi1|4930515B02Rik_chr4_140871158_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mir674|Rap1a-ps2_chr2_117200101_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mir674|Rap1a-ps2_chr2_117200101_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22507|Trhde_chr10_114071769_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22507|Trhde_chr10_114071769_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm2058/Gm26526|Gm26327_chr7_39605108_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm2058/Gm26526|Gm26327_chr7_39605108_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16198|D630002J18Rik/Gipc2_chr3_152165227_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16198|D630002J18Rik/Gipc2_chr3_152165227_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10040|Gm17641/1110032F04Rik_chr3_68818431_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10040|Gm17641/1110032F04Rik_chr3_68818431_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bag3|Gm7258_chr7_128573189_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bag3|Gm7258_chr7_128573189_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intron_Lasp1_chr11_97808239 |
GGGCTCACACAAACCCTGATTCTCTGCCACTTCCTCCCCCCAACCCCCCTTTGGAGAGTGGGGTGAGGTTCAGAGGAAAA AAAAAAAAGCCCAGGGCATTTAACCCTTTGGGTGCTGGACTGCTTTAG |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924063 |
AAGATGCAGGGCCAGGAATCTCCATGGTCTGCCTCTGGCTCTCCAGAGGGTGGCGCTGTAGGACAAGGAGCTGAGGGTCT ACACTGAGATCATGGAGATCTGGCGATGC |
| mm4_intergenic_Nlgn2|Tmem256_chr11_69837856 |
CACACCCCTCGCTTGCACCCGCCTGCCCACCCGCCCTCTGGAGTGTGGGGGGGGATTGAAGACAGTGAAAGGGGTGGGAG TGGACAGACGGACGGATGGACGTAGCGAACTAGAGCAGGG |
| mm4_intergenic_Hmg20a|Gm26868_chr9_56547330 |
TGTCTGGGAACTGGCAAAGGCAAGCAGTCCCCTCTTGCCAGGAATCTCCAAAGGGTGGGGTTCATGCCTGCTCCTCCCTC GAGCCCAGAACCTTCCACTGGGCTGTGAGATGGCTGGGTGGAAGAG |
| mm4_intron_Lypd6b_chr2_49866801 |
TAATCACATGGGCGCTGTGAGTATTAAGTACACAAGTGGGTGCAGAGGAGATACTCCGATTGAACTGCAAATCCTCATCA CATGAATGATCCCTCCTTCTGGAGAATCCATGGAAAGAGCTGTGCTCTGCAAGTCTC |
| mm4_intergenic_Hs3st3b1|Cox10/9630013K17Rik_chr11_63953002 |
ACAATTCTGAGGGAGCAGCCCACTTCCTGTGGGAGAACCTTGTCTGCGGCTGGTGTTTTCCTCTTTCTGGAGTGTCCGGG CACTAGCTGTCCTTGGTCCTGCAAGCAGGCTCAGAACCAGA |
| mm4_intron_Zfp64_chr2_168911037 |
GGGGCAAGTAGCTGAAGATGACGGAGGAATGTGTAGACATATAAAGGTATCCCCCTTCCCCGGGCTCTTCAGAAGTGGGG GATCTGCCAGTAGGAAGGCGGCACTCCACCTAAGGTAC |
| mm4_intergenic_Gm25115|Diap3_chr14_86510617 |
GCGATGCCAGAAGAGCAAGAGGGCACCGGCCTCTCCAGATGGAGGCTCTGGATGGTGCTGCACACTGCTGGTGTGCCAGC TTGAGGGGGGCTTGTCGTTGCCATTCATACACCATTAGGAACATCGCCATCATGCA |
| mm4_intron_Igf1r_chr7_68160317 |
TGCCCTTTAACCATGCCTGTGTGCCCCTCCTCTGGAGTGGCGGGGGAGGTCCCCTCCTTTCCCCCCATGGTGCTGTAATT GGAAATTTGGTTACTGCACTAAATTTTAAGTTTTGGCACTCATGGAGAGCAG |
| mm4_intron_Ip6k3_chr17_27157174 | TCCCAGATTCCCCATGTCCTGTCTGTCCTGGCTCTCCAGAGGGAGGCCCAGGAAGCCTCCCTTGGTCCTATGT |
| mm4_intergenic_Iscu|Tmem119_chr5_113781049 |
CCCAGCTGTCTTTGGAACCTCTACCCACCTCCACCCCTGGCCTCACTTCATGGAAACCTAATTCCCAGGAGTCTCCGAAG GGAGGAACACCCTCACTACCCTGGATCTGCTAGTGCAGAGTGGTTTCTGGACC |
| mm4_exon_Tmem233_chr5_116040634 |
TCCCGACTAGTGGGTGTTCATTTGCCAGGGACCCGCCTCTGGAGAGTCCTGGAGGAAAAGATCTTACAGTTTTAAAATCA AGGATCCATTCCTCAGAAACTTCTTTGGGAGTGTGTGCAGATGAG |
| mm4_intron_Gab3_chrX_74970886 |
GTGTCTGCCTGACTCTGGACGATAGAGTTCCTCAGGACTCAGAGGGCATTTTGAACAGTCCTAGTTGGACTCTCCAGAGG GTGGTTTGGTCCATAGGCTGAGTAGGGCTGGATGGATTCTGCAGGGTCACAGCAA |
| mm4_intron_Tet3_chr6_83441473 |
GCGCCTTCCAGTGTTTTGTTTTCCTGCTTCGGTTCTCCGGCCTCTGGAGATCCCCGGGACCCGCTCGCGGCCTGCACACG GCCTCCCCTCCCTTCCAGGCCCAGCGCTCTACCTCGGCCGGCCTCCACTACTGCATC |
| mm4_intron_Fam65a_chr8_105620729 |
ATCCGTGTCCTCCAGAGTGTGGCCAAGGAGTCCCTGGGCATGAGCTAGACCGGGGACCCTCCAGAGGGTGGCCAAGGAGT CCCTGGGCATGAGCTAGACAG |
| mm4_intron_Ppp2r5c_chr12_110490108 |
TTCAGGACCAAGGCCACATCCACCTGTTCCACTTTTCCTTTTGAGTCCTTCCAGTGCCAAAAGACATTATTCCTCCTCCC CCCACCCTTTGGAGAGAACAGGGAGCGAGCGCCCTCTGCAGGATTACAGA |
| mm4_intron_Camsap3_chr8_3590975 |
GGTAAGACCCAGTGTGGTCCAAGCAGCTGGGTCTGCAGGAGAAAGTTCCCAAGATTTTCCAGAGGGGGGTGGGTGAGGAA GGGGGGTTCCCCTGAGAAGCTGTCTTGAGACCACCAAGGGG |
| mm4_intergenic_C330021F23Rik|Gm10186/C330021F23Rik_chr8_3583111 |
CCCCTTGGTGGTCTCAAGACAGCTTCTCAGGGGAACCCCCCTTCCTCACCCACCCCCCTCTGGAAAATCTTGGGAACTTT CTCCTGCAGACCCAGCTGCTTGGACCACACTGGGTCTTACC |
| mm4_intergenic_4930407G08Rik|Slc1a7_chr4_107961964 |
AGGTGCAGGCATGAGTCTTCCACCAAATCACTTTCCTCCATCTGGTGAGGCCAGGGAGGCAAGGTAGGGCCCTTCTACAG AGGTT |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_128003406 |
AATGGTGGCTGGAACTCCTGATGGATGGGAGGAGTGCCACACCGGGCACTCCAGAGGGCAGCATATCTGCTTCCCACCAG CAGTGAGACCACTGTGTGCCTCAG |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_6430706 |
CCCATACCCCTTTTGGTCCCTTAGAGAACTTCTACCCATGGTTCCCCCCTCTACCCCAGCAATTCTCCAAACTCTGGAGA GTCCTGCGACCTCAGATGTCTTCCATCACCTATCTGTGCATGGAACTGGGAG |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823915 |
CACTACTGGGGCCACGATTGGTTGGAGAGCGGCCCCGCTCTGGAGACTCCCAGGATGCGCTCCTGCCCCGTCGGCTCGGC CTAGGAGGCGGGGCCGGGGCGGGGCGAGGGCTCGGCAGGCTCGCCCTGTATGTAA |
| mm4_exon_Olfr353_chr2_36890046 |
TCGCAGCTGCTACAACATCCTTCTCCGTGGTCTCTCCAGAGGAAGGGAACAGATAGACACTGAAGAGTGTTCCATAGAAA ACACAGACCACACAGAGATGGGAACTACAAGTAGAAAAGGCCTTTGAACTCCCGCCAGAA |
| mm4_intron_Pdzd2_chr15_12436492 |
TCCCCTCTTCCCTCAAAGGTAGCAATGTCCCAGTAACTGATGAAGAATGCAGATGTTTGCTTTGCTTCTCAAGTCCCCGG GCTCGACAGAGGTAGGTCAGTTCCCAAAGCTTAACCGTGCT |
| mm4_intergenic_Evc|Evc2_chr5_37337994 |
TCACAGAAGACCCTGTCCCTGGATAAGGGACATCTCTAGAGCCCAGAAGGCAGACTACCCCTTCCCCAGCTCCTCCCTCT GGGCACTCAGGGGAGCCCTAACACCCCTTGGGGGAGCTTTCTGCCCCAGCTTA |
| mm4_exon_Nos2_chr11_78940287 |
CCAAACTGCCTCACCCTCATCACTTGGCTTCTATAGGAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTC CCTCTGGAAAGACCGGGCTGTCACGGAGATCAATGTGGCTGTGCTCCA |
| mm4_intron_Ccdc85c_chr12_108245827 |
GTGATTCGACCTCCCTGAGCCGCAGAGCTGGGACTCTCCAGAGGGAAGCCCTGTCAGTCAGCGTGGGGGGCGGGAGAATG TTGGTCCTCGTTTAGACACAGGCTGTGGGACAGACGGTGTCCTTCATTGCGAT |
| mm4_intron_Ass1_chr2_31509700 |
GCGTGTTCATGCATGTGTGTGCACAGCCGTCCCCCTCCCCCGACTCCCCAGTGGAAGGGAGGAAGGGCTATTTGGGGCCC TGGGCTCCACCGTAGTTGGTTTAATTACAGGTTTTCAACTGCAGGCC |
| mm4_exon_Glp1r_chr17_30901944 |
GCTATGACCCAGTCCTGAGCGCCCAGCCATGGCCAGCACCCCAAGCCTCCTGCGCCTGGCGCTCCTGCTGCTCGGGGCGG TGGGCAGGGCCGGCCCCCGCCCTCAGGTGAGCCCTGGGAACCCCGGTGACAAATGGGAG |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138508596 |
TCGCCTTCTTGCTGTGACATCCTTCCCTGCTGGCCCAGTGGTCCTCATCCCTGGGGTCTCCTGAGGGTGGCCAGATGACA TCAGAGGAGCCACAGTCC |
| mm3_intergenic_Gm5507|Redrum_chr18_54202223 |
TCCTGAGACAACAGAAGATGGTTGAATGACAAAGTCAAAGCAGTAATCACAGGCCACAGACTCTCCAGAGGGCGAAATTC CCATCCTTGAAATTTCCTCTTTCCTCCTTATCTCCCCCATGGGTTTGCAAGCAC |
| mm4_exon_Dok7_chr5_35079662 |
GGGACAGGCAACGGAAGGCCCAGGCAGTGACGCTGCGCTGCCGAGTCCATCCCCTGGCGAGTCCTGGGAAGCAGGCAGCC CCCATGCAGGGCCGCCTCCAGCTTTCTTTTTGTCATGTTCAATCTGTGGCGGACTCAAGG |
| mm4_exon_Col26a1_chr5_136743181 |
AGGACCTCCTCGCTTCATCTTGAGATTGGCTCTCAGGGCTGCATCCTGCCCAGAGCCACCCTCAGGAGAGGCCAGGGGAT CTGGAAGGAGGGCAGAAGGAAC |
| mm4_intron_Lama3_chr18_12359152 |
CCACATCACCTTGGAGCTGTGTCTATTCAAATCTCCTTCCATGTTAGCCCCGGAGTTTCCTGAGGGCGGGACTGTTTCTT CCTGCTCCTGGACTTTCTTGTGGCAGCT |
| mm4_intergenic_Atp2c1|Gm5621_chr9_105553787 |
CTTTGGTCACAGGGCCTAGGGTGCTTAAGATCCATCTCCCTGCCTCTGCAGAGTCAGGTGACAGCCTAAGTAGCAATTAT TTCCCTTTTCCAACAAAGAGAAACGAGATTAGAGTTTAGCACCCGGGC |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911237 |
CGGGGTCAGTGGTTCACTCCCCCTCCCCGGACTCCCCTGCGGAGGGGGCTGACAGCGACGGTCGCGGGGCCTGGTGTCTC GGGGCTGTC |
| mm4_intergenic_Gm20424|Lbx1_chr19_45201005 |
GGCATTTCAGACTGGGCTCTATTGCTCCTGAAATGCAACCCCCAAGGCCAACCTCTCCCTCTGGAGGGTCCAGGTGAAGG GGTTTGCAGGAGGAAA |
| mm4_intergenic_Gkn3|Bmp10_chr6_87412077 |
TGGTGAGCAGGGAGACAAAGAATGTCAGGTGATCCAAAAACATATCTTAAGGCCAGCTGTCCCAGGACACTGCAGGGGGT GGGCGTGGAAGGCTTCTAAGCCAGCCATTATCAGGGG |
| mm4_intron_Dgat2_chr7_99176680 |
AGGACCAAGCTCAGAGGGATGTCCCCGGACTGTCCTGGGGAGGGGATACAACAGCTCAGATGAGGTATCAGCCTAACATA AGGAAGTCATGGGCCCTG |
| mm4_intron_Tsku_chr7_98355066 |
ACAGCCCAGATGTTGCTCTCCTCTGGGCCCTGGCCCTGGACTCTGCACAGGGAGGCAAAGTCTATCCTGCCATCCTCACA CAAGCCAACAAACCCCTG |