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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313786_F | TCGTCGGCAGCGTCCCTTGTCGCCTTAGCTCCTC | 60.1 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313786_R | GTCTCGTGGGCTCGGGTCCTTTCGGCTCTCCATCC | 60.1 | 1.00 |
| mm4_intergenic_Hook3|Hook3/1700047A11Rik_chr8_26095652_F | Primer3: not found at this Tm | N.d. | 0.52 |
| mm4_intergenic_Hook3|Hook3/1700047A11Rik_chr8_26095652_R | Primer3: not found at this Tm | N.d. | 0.52 |
| mm4_intron_D030068K23Rik_chr8_109135220_F | TCGTCGGCAGCGTCTCCAAAATGGGTTTCTTCTGTCT | 58.1 | 0.51 |
| mm4_intron_D030068K23Rik_chr8_109135220_R | GTCTCGTGGGCTCGGCGTGGCTGCCTTCTTTGGAT | 60.9 | 0.51 |
| mm4_intron_Grm7_chr6_111103689_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intron_Grm7_chr6_111103689_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334540_F | TCGTCGGCAGCGTCCCTTGACTCTAGGGGAGCCT | 60.0 | 0.44 |
| mm4_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334540_R | GTCTCGTGGGCTCGGAATGGCTCATGCTGTGTCCA | 59.9 | 0.44 |
| mm4_intergenic_Gm14889|Gm14888_chrX_58840112_F | TCGTCGGCAGCGTCAGCACTGATCTTATGAGAATGAGGA | 59.3 | 0.43 |
| mm4_intergenic_Gm14889|Gm14888_chrX_58840112_R | GTCTCGTGGGCTCGGAAGCAGATAAAACGCCTTAAGG | 57.0 | 0.43 |
| mm4_intron_Pggt1b_chr18_46276006_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intron_Pggt1b_chr18_46276006_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Etv3|Gm25167_chr3_87574401_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Etv3|Gm25167_chr3_87574401_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Gm12130|Gm23520_chr11_38665787_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Gm12130|Gm23520_chr11_38665787_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Efcab3_chr11_105099777_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Efcab3_chr11_105099777_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_exon_Ccp110_chr7_118722503_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_exon_Ccp110_chr7_118722503_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Orc3_chr4_34572321_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Orc3_chr4_34572321_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868441_F | TCGTCGGCAGCGTCCACTGACGTACTTAACAGTGAGGA | 60.0 | 0.28 |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868441_R | GTCTCGTGGGCTCGGGAACCTCGGTCATGCTGGAA | 60.0 | 0.28 |
| mm4_intergenic_Gm12469|Gm25687_chr4_54281918_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm12469|Gm25687_chr4_54281918_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Ralgps1|Gm13537_chr2_33318934_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Ralgps1|Gm13537_chr2_33318934_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Bai3_chr1_25565225_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Bai3_chr1_25565225_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_Gm15262|Prkaca-ps1_chrX_161457334_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3_intergenic_Gm15262|Prkaca-ps1_chrX_161457334_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3_intergenic_Gm7275|Gm7204_chr16_48104808_F | TCGTCGGCAGCGTCTGGCTTTGATTCTGGCACTCA | 60.2 | 0.22 |
| mm3_intergenic_Gm7275|Gm7204_chr16_48104808_R | GTCTCGTGGGCTCGGGGGAGCTACCATTTGCAGCT | 60.3 | 0.22 |
| mm3_intergenic_Pvrl3|Gm6912_chr16_46589146_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intergenic_Pvrl3|Gm6912_chr16_46589146_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intergenic_Gm26819/Med10|Gm6132_chr13_69862963_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Gm26819/Med10|Gm6132_chr13_69862963_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42410645_F | TCGTCGGCAGCGTCCCAGGCAGAGGTCTGTTCTC | 59.7 | 0.19 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42410645_R | GTCTCGTGGGCTCGGGAAGCACCTCGTGAAACCCT | 60.2 | 0.19 |
| mm4_intergenic_Gm24063|Rps15a_chr7_117962858_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm24063|Rps15a_chr7_117962858_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Gm26162|Gm12887_chr4_121525004_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_Gm26162|Gm12887_chr4_121525004_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm24773|Ncam2_chr16_81180322_F | TCGTCGGCAGCGTCGTCAGGGTCCGTTGTTCACT | 59.8 | 0.17 |
| mm4_intergenic_Gm24773|Ncam2_chr16_81180322_R | GTCTCGTGGGCTCGGCGCTATTTCAAATCCACGGGG | 59.6 | 0.17 |
| mm4_intergenic_Gm5899|Gm23884_chr7_94468392_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm5899|Gm23884_chr7_94468392_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105459286_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105459286_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Spats2l_chr1_57805288_F | TCGTCGGCAGCGTCAGGGATAGATGATGCTGAACCAC | 59.9 | 0.15 |
| mm4_intron_Spats2l_chr1_57805288_R | GTCTCGTGGGCTCGGTGTGGCTACCATGTTACCCAC | 59.9 | 0.15 |
| mm4_intron_Cckbr_chr7_105428294_F | TCGTCGGCAGCGTCGGTTACTCACCAAAGCTGTTGA | 58.7 | 0.15 |
| mm4_intron_Cckbr_chr7_105428294_R | GTCTCGTGGGCTCGGACCCCTCCCTCTAGTGAAGAC | 59.9 | 0.15 |
| mm4_intergenic_Gm23558|Galc_chr12_98145384_F | TCGTCGGCAGCGTCACATGCTAAGTGCTTTAAACACCA | 59.4 | 0.14 |
| mm4_intergenic_Gm23558|Galc_chr12_98145384_R | GTCTCGTGGGCTCGGACTCTGGAAACCACATGTACAT | 57.3 | 0.14 |
| mm4_intron_Ipo5_chr14_120912981_F | TCGTCGGCAGCGTCACTTTGTGCAAACTCGAGGT | 57.9 | 0.13 |
| mm4_intron_Ipo5_chr14_120912981_R | GTCTCGTGGGCTCGGTCTGCTCTGCAACACATGGA | 59.6 | 0.13 |
| mm4_intergenic_Ranbp17|Anp32-ps_chr11_33349558_F | TCGTCGGCAGCGTCCCTGTCTCCCCTGCAATCTC | 59.8 | 0.13 |
| mm4_intergenic_Ranbp17|Anp32-ps_chr11_33349558_R | GTCTCGTGGGCTCGGACTGATAGCAGCTTCCTTGGG | 59.7 | 0.13 |
| mm4_exon_Zc3h14_chr12_98747500_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Zc3h14_chr12_98747500_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Fbln1_chr15_85253434_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Fbln1_chr15_85253434_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Selp_chr1_164137709_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Selp_chr1_164137709_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Helt|Acsl1_chr8_46410255_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Helt|Acsl1_chr8_46410255_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Olig2|Olig1_chr16_91267127_F | TCGTCGGCAGCGTCTCACTTTGCTTCCCGTCTGG | 60.2 | 0.12 |
| mm4_intergenic_Olig2|Olig1_chr16_91267127_R | GTCTCGTGGGCTCGGACTGAAATATGCAAACGCCCG | 59.8 | 0.12 |
| mm4_intron_Rasgef1c_chr11_49967637_F | TCGTCGGCAGCGTCCCTCAATCTGGGCCTGTCTG | 60.1 | 0.12 |
| mm4_intron_Rasgef1c_chr11_49967637_R | GTCTCGTGGGCTCGGGGCCTGGACTTTTAACATGCA | 59.1 | 0.12 |
| mm4_intergenic_Gm22236|Csrp2_chr10_110919062_F | TCGTCGGCAGCGTCTCAAACTCCTGCCGCAATCT | 59.9 | 0.11 |
| mm4_intergenic_Gm22236|Csrp2_chr10_110919062_R | GTCTCGTGGGCTCGGTTATGTCGTATGCTGGGCCC | 59.8 | 0.11 |
| mm4_intron_Lama3_chr18_12385709_F | TCGTCGGCAGCGTCCAGTCCCACTTGCTCCTCTG | 60.0 | 0.11 |
| mm4_intron_Lama3_chr18_12385709_R | GTCTCGTGGGCTCGGACGTAGTGGCCAGGACAAAG | 59.9 | 0.11 |
| mm4_intergenic_Gm23191|Ascl1_chr10_87389288_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm23191|Ascl1_chr10_87389288_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Ttll7|Gm23131_chr3_147124891_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Ttll7|Gm23131_chr3_147124891_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Frmpd4_chrX_167873834_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Frmpd4_chrX_167873834_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Chek1_chr9_36725815_F | TCGTCGGCAGCGTCACTCTCCATAGGCACCTTCTC | 58.6 | 0.10 |
| mm4_exon_Chek1_chr9_36725815_R | GTCTCGTGGGCTCGGGGCAGTGCCTTTTGTGGAAG | 59.9 | 0.10 |
| mm4_intergenic_Pdcl3|mmu-mir-6349_chr1_39094426_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Pdcl3|mmu-mir-6349_chr1_39094426_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Prickle1|Adamts20_chr15_93987789_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Prickle1|Adamts20_chr15_93987789_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111535923_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111535923_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Shank3_chr15_89552071_F | TCGTCGGCAGCGTCACCCTTACTTTGATGTCTGTGGG | 60.2 | 0.10 |
| mm4_intron_Shank3_chr15_89552071_R | GTCTCGTGGGCTCGGACACGCTTTGGACACTTCTCT | 59.8 | 0.10 |
| mm4_intergenic_Gm23022|Rab28_chr5_40773576_F | TCGTCGGCAGCGTCAGCTGAAATTGGCCTCCAGT | 59.5 | 0.09 |
| mm4_intergenic_Gm23022|Rab28_chr5_40773576_R | GTCTCGTGGGCTCGGTGAGTAGAACTTGGCAATGGCA | 60.2 | 0.09 |
| mm4_intergenic_Chmp2b|Vgll3_chr16_65709443_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Chmp2b|Vgll3_chr16_65709443_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Tnrc6a|Slc5a11_chr7_123197570_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Tnrc6a|Slc5a11_chr7_123197570_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tbc1d21_chr9_58361892_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tbc1d21_chr9_58361892_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm14628|Gm14604_chrX_44061389_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm14628|Gm14604_chrX_44061389_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm20319|Gmnc_chr16_26876838_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm20319|Gmnc_chr16_26876838_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm12644|Elavl2_chr4_91229064_F | TCGTCGGCAGCGTCACCCTGCCACTTCTCCTCTT | 60.4 | 0.07 |
| mm4_intergenic_Gm12644|Elavl2_chr4_91229064_R | GTCTCGTGGGCTCGGCCACAGCATCTTCATGAGACG | 59.0 | 0.07 |
| mm4_intron_Ctnna3_chr10_64252010_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ctnna3_chr10_64252010_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AC132241.1|Gm9898_chr1_30392930_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AC132241.1|Gm9898_chr1_30392930_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cep68_chr11_20236649_F | TCGTCGGCAGCGTCGCTGGAACCTCTCTATGCCC | 59.8 | 0.07 |
| mm4_intron_Cep68_chr11_20236649_R | GTCTCGTGGGCTCGGAATAAGAATCATCAAGGCCTTGTAG | 57.1 | 0.07 |
| mm3_intron_Atp1a2_chr1_172289262_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Atp1a2_chr1_172289262_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Lpl|Slc18a1_chr8_69014011_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Lpl|Slc18a1_chr8_69014011_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mrgpra2b|Mrgpra3_chr7_47553726_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mrgpra2b|Mrgpra3_chr7_47553726_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Neurog1|Cxcl14_chr13_56259423_F | TCGTCGGCAGCGTCGCTGCTGCTGGTCCATTCTT | 60.9 | 0.06 |
| mm4_intergenic_Neurog1|Cxcl14_chr13_56259423_R | GTCTCGTGGGCTCGGCGCCAGACTACGGTGAGAAA | 59.7 | 0.06 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516366_F | TCGTCGGCAGCGTCGATCGAGTGTGGAAGGGGAC | 59.8 | 0.06 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516366_R | GTCTCGTGGGCTCGGTCGAACAATCCCCGACACTG | 60.0 | 0.06 |
| mm4_intergenic_Lbr|Gm26004_chr1_181840036_F | TCGTCGGCAGCGTCGGTTCATTACTGAGCCTGCCT | 60.0 | 0.06 |
| mm4_intergenic_Lbr|Gm26004_chr1_181840036_R | GTCTCGTGGGCTCGGCGCTTCTGCCAGTCTGACTT | 60.3 | 0.06 |
| mm4_intergenic_Gm22331|Gm24937_chr1_113368937_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm22331|Gm24937_chr1_113368937_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm11822|1110037F02Rik_chr4_11467301_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11822|1110037F02Rik_chr4_11467301_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tmpo|Rmst_chr10_92015956_F | TCGTCGGCAGCGTCTCTGGGACAAAATGCACCTT | 57.6 | 0.04 |
| mm4_intergenic_Tmpo|Rmst_chr10_92015956_R | GTCTCGTGGGCTCGGCTGTTAGGCTCATGCTTGGG | 58.6 | 0.04 |
| mm4_intergenic_Cadm2|Gm24681_chr16_68105911_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Cadm2|Gm24681_chr16_68105911_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Grik2|Gm26543_chr10_49174363_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Grik2|Gm26543_chr10_49174363_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_1810041L15Rik_chr15_84384207_F | TCGTCGGCAGCGTCACCAGCCCCAGAGAGAACTA | 59.5 | 0.04 |
| mm4_exon_1810041L15Rik_chr15_84384207_R | GTCTCGTGGGCTCGGGAGGAAGTACCACCTGTGCC | 60.0 | 0.04 |
| mm4_intergenic_Gm26327|Gm6851_chr7_39841532_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26327|Gm6851_chr7_39841532_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Dhrs7c_chr11_67811761_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Dhrs7c_chr11_67811761_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22282|Dnajc5b_chr3_19445165_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22282|Dnajc5b_chr3_19445165_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tars|Npr3_chr15_11767528_F | TCGTCGGCAGCGTCACACAGCATCACAGAAGCTCA | 59.9 | 0.04 |
| mm4_intergenic_Tars|Npr3_chr15_11767528_R | GTCTCGTGGGCTCGGAGGGGAAGATGGGTGGAGTA | 59.2 | 0.04 |
| mm4_intron_Arl6ip5_chr6_97228341_F | TCGTCGGCAGCGTCACCAATGTTTCTGTGGGGCA | 60.1 | 0.04 |
| mm4_intron_Arl6ip5_chr6_97228341_R | GTCTCGTGGGCTCGGTACCCACCATCGCTTCCATG | 59.8 | 0.04 |
| mm4_intron_Lgi2_chr5_52552282_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Lgi2_chr5_52552282_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Olfr1154_chr2_87903222_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Olfr1154_chr2_87903222_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Lrrc4c|Gm13803_chr2_97860814_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Lrrc4c|Gm13803_chr2_97860814_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_4930593A02Rik_chr3_58729997_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_4930593A02Rik_chr3_58729997_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Dlx5_chr6_6879643_F | TCGTCGGCAGCGTCACCTTCTCTGTAAAGCGGCC | 60.0 | 0.03 |
| mm4_exon_Dlx5_chr6_6879643_R | GTCTCGTGGGCTCGGGAGCCAGAGGTGAGGATGGT | 60.9 | 0.03 |
| mm4_intergenic_Nek7|Lhx9_chr1_138771301_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nek7|Lhx9_chr1_138771301_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Epb4.1l3_chr17_69232133_F | TCGTCGGCAGCGTCGTTTTAAAGGCGGCACCCTG | 60.0 | 0.03 |
| mm4_intron_Epb4.1l3_chr17_69232133_R | GTCTCGTGGGCTCGGTGAGGAGGGGAAAGCTCACT | 60.1 | 0.03 |
| mm4_intergenic_Gm14062|Btbd3_chr2_138086164_F | TCGTCGGCAGCGTCAACACACAGAGGGCAAACCA | 60.0 | 0.03 |
| mm4_intergenic_Gm14062|Btbd3_chr2_138086164_R | GTCTCGTGGGCTCGGGAAGGTGGGAGATGTGGGC | 60.0 | 0.03 |
| mm4_intergenic_Dmrta2|Elavl4_chr4_110118310_F | TCGTCGGCAGCGTCCTGGACCTGAAACTGACCCC | 59.9 | 0.03 |
| mm4_intergenic_Dmrta2|Elavl4_chr4_110118310_R | GTCTCGTGGGCTCGGTGCTGGATGATGAAGACAGATT | 57.5 | 0.03 |
| mm4_intergenic_A730085K08Rik|9530059O14Rik_chr9_122499547_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_A730085K08Rik|9530059O14Rik_chr9_122499547_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pqlc2_chr4_139300629_F | TCGTCGGCAGCGTCGCTTGGCACTCAAAACTCCC | 59.6 | 0.03 |
| mm4_intron_Pqlc2_chr4_139300629_R | GTCTCGTGGGCTCGGGGACCCCTACTGGAGGCTTA | 60.0 | 0.03 |
| mm4_intron_Cpa5_chr6_30626576_F | TCGTCGGCAGCGTCAAAGCCGTCTTGCCCAGTAA | 59.8 | 0.03 |
| mm4_intron_Cpa5_chr6_30626576_R | GTCTCGTGGGCTCGGATGGGCTGACATGGAACTGG | 60.0 | 0.03 |
| mm4_intergenic_Gm7936|Gm24396_chr18_30544311_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm7936|Gm24396_chr18_30544311_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Grip1_chr10_120045735_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Grip1_chr10_120045735_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm14923|Gm6427_chrX_112782926_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm14923|Gm6427_chrX_112782926_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lzts3_chr2_130639190_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lzts3_chr2_130639190_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Helz_chr11_107563240_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Helz_chr11_107563240_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Prickle1|Adamts20_chr15_94047322_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Prickle1|Adamts20_chr15_94047322_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm10617|Gm25200_chr8_117950289_F | TCGTCGGCAGCGTCCACCTTGGCTCTTACTGGCA | 59.9 | 0.02 |
| mm4_intergenic_Gm10617|Gm25200_chr8_117950289_R | GTCTCGTGGGCTCGGAACACAGACCTGAGCAGAGC | 59.9 | 0.02 |
| mm4_intron_Dync1i1_chr6_5893609_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dync1i1_chr6_5893609_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm6132|Gm26018_chr13_70015940_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm6132|Gm26018_chr13_70015940_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm8609|Gm22543_chr16_39967359_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm8609|Gm22543_chr16_39967359_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Dppa2|n-R5s36_chr16_48385279_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Dppa2|n-R5s36_chr16_48385279_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23345|Usp47_chr7_111950232_F | TCGTCGGCAGCGTCCAGAGACATAGACTTCAAGGCAGT | 60.0 | 0.01 |
| mm4_intergenic_Gm23345|Usp47_chr7_111950232_R | GTCTCGTGGGCTCGGCACAGTGGTGGAGGGGTTTT | 60.1 | 0.01 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42250083_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42250083_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Hhat_chr1_192769959_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Hhat_chr1_192769959_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm3898|Gm23326_chr9_43896214_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm3898|Gm23326_chr9_43896214_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Prkdc_chr16_15799519_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Prkdc_chr16_15799519_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Auts2_chr5_131707748_F | TCGTCGGCAGCGTCGTTTTGGCAAGCGGTCTGG | 60.0 | 0.01 |
| mm3_intron_Auts2_chr5_131707748_R | GTCTCGTGGGCTCGGTCTGGAGCCTGTTCAAACTCA | 59.2 | 0.01 |
| mm4_intron_Fam133b_chr5_3548226_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam133b_chr5_3548226_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Zswim7_chr11_62274070_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Zswim7_chr11_62274070_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Cacng2|Cacng2/A730060N03Rik_chr15_78079247_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Cacng2|Cacng2/A730060N03Rik_chr15_78079247_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11361|2610307P16Rik_chr13_28386172_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11361|2610307P16Rik_chr13_28386172_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptdss1|Zfp712_chr13_67002185_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptdss1|Zfp712_chr13_67002185_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_5730522E02Rik|mmu-mir-6372_chr11_26159264_F | TCGTCGGCAGCGTCAGAGTTGGGTAGGGAGCTGT | 59.8 | 0.00 |
| mm4_intergenic_5730522E02Rik|mmu-mir-6372_chr11_26159264_R | GTCTCGTGGGCTCGGATTTATCAGGCTGGCACGGT | 59.7 | 0.00 |
| mm4_intergenic_Ptch1|Ercc6l2_chr13_63688101_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptch1|Ercc6l2_chr13_63688101_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26863|Azin1_chr15_38484385_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26863|Azin1_chr15_38484385_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Myom3_chr4_135783502_F | TCGTCGGCAGCGTCAGGTCTCTGCTGAGCCTCTT | 60.2 | 0.00 |
| mm4_intron_Myom3_chr4_135783502_R | GTCTCGTGGGCTCGGGAGCTTGGCAAGAGACGACA | 60.3 | 0.00 |
| mm4_intergenic_Fat3|Gm5611_chr9_16871662_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fat3|Gm5611_chr9_16871662_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_mmu-mir-6238|AC161571.1_chr7_53920560_F | TCGTCGGCAGCGTCAGAGTTTGGCTACTTGTAAAACTGA | 58.6 | 0.00 |
| mm4_intergenic_mmu-mir-6238|AC161571.1_chr7_53920560_R | GTCTCGTGGGCTCGGACCATTGGTGAGAAAAGCTGA | 57.7 | 0.00 |
| mm4_intergenic_Gm25329|mmu-mir-6351_chr1_73826931_F | TCGTCGGCAGCGTCGGCTGACGGAAACACATCCT | 60.3 | 0.00 |
| mm4_intergenic_Gm25329|mmu-mir-6351_chr1_73826931_R | GTCTCGTGGGCTCGGGCCGATGCTCAATGCTTCTG | 59.9 | 0.00 |
| mm4_intron_Lhx6_chr2_36094177_F | TCGTCGGCAGCGTCGACCACGAACTTGGGTGCTA | 59.9 | 0.00 |
| mm4_intron_Lhx6_chr2_36094177_R | GTCTCGTGGGCTCGGTTGCCGCATCCATTACGACA | 60.3 | 0.00 |
| mm4_intron_St18_chr1_6829809_F | TCGTCGGCAGCGTCAAGTTCTCCCAGTGCCATCC | 59.6 | 0.00 |
| mm4_intron_St18_chr1_6829809_R | GTCTCGTGGGCTCGGATTCTGCCCACAACAGCTCC | 60.6 | 0.00 |
| mm4_intergenic_Rarb|Thrb_chr14_16797503_F | TCGTCGGCAGCGTCTTCTGCTCTGGTCTGTCTGC | 59.6 | 0.00 |
| mm4_intergenic_Rarb|Thrb_chr14_16797503_R | GTCTCGTGGGCTCGGGGGACTGGGAAATCACGACA | 59.6 | 0.00 |
| mm4_intergenic_AC147224.1|Cdh12_chr15_21108241_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AC147224.1|Cdh12_chr15_21108241_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313786 |
CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTC CCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGAC |
| mm4_intron_D030068K23Rik_chr8_109135220 |
TCCAAAATGGGTTTCTTCTGTCTATCTTCTGCTAGATAATATTACTGGAAAGAAGAGCTTACTTAGGACAGGAAAATAAT TTTTGCAAAGACTCTGTCATTCATTATATTCTATTTGAATATCCAAAGAAGGCAGCCACG |
| mm4_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334540 |
CCTTGACTCTAGGGGAGCCTCTGTCTCATGGTGGGATTCAGAGCTAACTTGGGGTTGCCCTCTTGTCCTTCTGGACACAG CATGAGCCATT |
| mm4_intergenic_Gm14889|Gm14888_chrX_58840112 |
AGCACTGATCTTATGAGAATGAGGAGAAACCCAAAGTAAGCCCTTCTTCCCAGAAGCATTTCTGCCTTAAGGCGTTTTAT CTGCTT |
| mm4_intergenic_Higd1a|Ackr2_chr9_121868441 |
CACTGACGTACTTAACAGTGAGGAAAAACATGGGGTTTGGAGCTCACTTGGGGAAGCATTGGTGCTGTTCCAGCATGACC GAGGTTC |
| mm3_intergenic_Gm7275|Gm7204_chr16_48104808 |
TGGCTTTGATTCTGGCACTCATTCCTGAATTGGTTCAGTTGATACAAAAAGTGGTTATGGCATTAGGAGCTTACATGGGT GCATGTTGTGGTGCAGAAGCTGCAAATGGTAGCTCCC |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42410645 |
CCAGGCAGAGGTCTGTTCTCTTTAGGAATTGAATACTGAAGATGGTGTTCTCAAGTAAGCTCCTGATTCCGAATCCCCAC CCACAGGCCTCCTACCAGGCTCCCTCCTCCAGCCCAGGGTTTCACGAGGTGCTTC |
| mm4_intergenic_Gm24773|Ncam2_chr16_81180322 |
GTCAGGGTCCGTTGTTCACTTGTTGATATTGGAGTCACTGGAAGTAGAAGCTGACTTGGGCTCGGTGAGGTATATGTCTT TGTGTCCACTAATATAAGTTGTTATTCCCCCGTGGATTTGAAATAGCG |
| mm4_intron_Spats2l_chr1_57805288 |
AGGGATAGATGATGCTGAACCACATTAACTCTGGAGTTAGGAGCCAACTTGGGTAAATTGAGATCATCCAATGTGTGGAT CCCAATGATGAAGAAACAAGTAACCAAGTGGGTAACATGGTAGCCACA |
| mm4_intron_Cckbr_chr7_105428294 |
GGTTACTCACCAAAGCTGTTGAATCCTGGGATCTGGAACTTACCTTGGAGATCTGAGCTGGAAATAGAGACTTGAAAAGT CTTCACTAGAGGGAGGGGT |
| mm4_intergenic_Gm23558|Galc_chr12_98145384 |
ACATGCTAAGTGCTTTAAACACCATTATGGCTTATTTAGATGTGATTTGGAGCTTATTTGGGTGTAATTTGAAACTTATT CATTGCCTCATCCAATTAATGTTAATAATGTACATGTGGTTTCCAGAGT |
| mm4_intron_Ipo5_chr14_120912981 |
ACTTTGTGCAAACTCGAGGTAATTTTTTTAAACCAAAGCAAGTTCTTAATCCCACCCTATAATAGAAATTTCCATGTGTT GCAGAGCAGA |
| mm4_intergenic_Ranbp17|Anp32-ps_chr11_33349558 |
CCTGTCTCCCCTGCAATCTCTGCATCTGCTTACTGGTGATCATGTGATGTCTTCAACTGATGCACAAGACTAATAACCTG AAATCAGGAGCTTACTGAGGCTCCCAAGGAAGCTGCTATCAGT |
| mm4_intergenic_Olig2|Olig1_chr16_91267127 |
TCACTTTGCTTCCCGTCTGGTACGATTTTGTTTTTCAACCCAGCTTTTTCTTCCAGCCACCCCCTCTCTTCCCAAGTGGG CTCCTATTGCCAGAGTCCTCTCTGAGTGAGCACCTTCGGGCGTTTGCATATTTCAGT |
| mm4_intron_Rasgef1c_chr11_49967637 |
CCTCAATCTGGGCCTGTCTGCCTCAGGCAGCTGGGGTGTGTAGCTTACTTGGGGCTTGGAGATAAAAAGGGTCTAACCTT TAAGAATTGAGAAATTGCATGTTAAAAGTCCAGGCC |
| mm4_intergenic_Gm22236|Csrp2_chr10_110919062 |
TCAAACTCCTGCCGCAATCTGGGGGAGATGTCAATCACTACTTGCACTGTTTTTCAACAGTCCTTGGTGGACAGAGAATC TGGCTGCAATTACGAGCTTATTTTGGATTGGGCCCAGCATACGACATAA |
| mm4_intron_Lama3_chr18_12385709 |
CAGTCCCACTTGCTCCTCTGCTGGCTGTGGGTGCACCTTCTCAGTGCCTCCCAAGCAAGTTCCTGACCCCAGCTTTGTCC TGGCCACTACGT |
| mm4_exon_Chek1_chr9_36725815 | ACTCTCCATAGGCACCTTCTCCCAAAGTTTGCACCAAATCCCAGTCTTCCACAAAAGGCACTGCC |
| mm4_intron_Shank3_chr15_89552071 |
ACCCTTACTTTGATGTCTGTGGGGGCTGTGCCTCAGCTCTGGCTTTGTAGCCAGTGGGGGCTGGAATCTGGCATTTGGGT CTTACTTTGGCATACAGATCACTAATGAGCCTTTCCCTTAGAGAAGTGTCCAAAGCGTGT |
| mm4_intergenic_Gm23022|Rab28_chr5_40773576 |
AGCTGAAATTGGCCTCCAGTCTTCGATAGTGTAAAAGAAAATAAAACCGTATATGAAAGAAAATTGCTGTGACTTGGAGC TTATTTGGGAGAAATTGTTGCCATTGCCAAGTTCTACTCA |
| mm4_intergenic_Gm12644|Elavl2_chr4_91229064 |
ACCCTGCCACTTCTCCTCTTATGAATTTCCTGTTATAAGGAGCTAACTTTGGGAAAACCACGAGGTTTTTTTATTCCCGC TTTCTGTTCATATAGGCAAGTAGCATAATCAGTGTTGACGTCTCATGAAGATGCTGTGG |
| mm4_intron_Cep68_chr11_20236649 |
GCTGGAACCTCTCTATGCCCCTCTGTTCACCTCCATGCAACACCTTCGTTCGCATCAGAGTAGCTATTTTCTGAAGAGCT CATCCCAAGCAAGTTCCTCCTCCCAGCTACAAGGCCTTGATGATTCTTATT |
| mm4_intergenic_Neurog1|Cxcl14_chr13_56259423 |
GCTGCTGCTGGTCCATTCTTACTTAAAGAAGTTTTAATATGGGATAAATGACCGGGAATTAGGAGCTGACGTGGGAGGGA CTAAAGAAACCGTGTCGTTCCTTTTCTCACCGTAGTCTGGCG |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516366 |
GATCGAGTGTGGAAGGGGACCCAGGAGAGGCAGAAAGTAAGGTGAAGCTGACATTAGGAGCTGACGTAGGAGAGATGGAT TATAAGCTGGTGCTAACACAGCACAGGGATGATGCCAGTGTCGGGGATTGTTCGA |
| mm4_intergenic_Lbr|Gm26004_chr1_181840036 |
GGTTCATTACTGAGCCTGCCTCAGTCCCCCTAACTTGTGAGATAGCATCTCTGGGATAAGGAGTATGCTTGGGAGCTGTA GGAATGAAGTCAGACTGGCAGAAGCG |
| mm4_intergenic_Tmpo|Rmst_chr10_92015956 |
TCTGGGACAAAATGCACCTTATTAAGCTTGTCTTAGGGGCTTACTTCGGAAATAACCTCTGGTTGCTAGAATTAAGATGC TAGTAGAAATTTGAGCTAAAGACAACTGTGAAAAATCCTGCCCAAGCATGAGCCTAACAG |
| mm4_exon_1810041L15Rik_chr15_84384207 |
ACCAGCCCCAGAGAGAACTATTGCTTGCAGACAGGTGGCTCAGAGCCCCTTTCCTGCACCCTGTCCCCGGACCCTGAGTT TGCTCCTAATCTCAGAAGTTCACGGAAGGGGGTTCTGGGCACAGGTGGTACTTCCTC |
| mm4_intergenic_Tars|Npr3_chr15_11767528 |
ACACAGCATCACAGAAGCTCAACAGATTTAAACCCAGCAGATGTACTGCAAGAAGTTCCCAAGGAAGCTCCTCGGCCCAG AAGGCAGTGTCTCTCTCCTTTTCTGCTTTACTCCACCCATCTTCCCCT |
| mm4_intron_Arl6ip5_chr6_97228341 |
ACCAATGTTTCTGTGGGGCATAAGGATTAGATCACTTACTGCGATCAGCAGCTTGCTTTGGGCTTTCATGTCAAGGAAGC GATGGCGGGTAGGGATGACTGGGCTCCATGGAAGCGATGGTGGGTA |
| mm4_exon_Dlx5_chr6_6879643 |
ACCTTCTCTGTAAAGCGGCCAGCTGAAAGCTGGAATAAATAGTCCTGGGTTTACGAACTTTCTTTGGTTTACCATTCACC ATCCTCACCTCTGGCTC |
| mm4_intron_Epb4.1l3_chr17_69232133 |
GTTTTAAAGGCGGCACCCTGCCTCAGGATTAATCCAACAAGCTGTGAGTAGGAACTTCCTTAGGATCCAGCCAGCAGGCT TCTCTGGCACAGAGGCTGCTTCCTTGCTTACCCGGAGAAAGTGAGCTTTCCCCTCCTCA |
| mm4_intergenic_Gm14062|Btbd3_chr2_138086164 | AACACACAGAGGGCAAACCAGGTATCAAAAATTAGGAGCTTACTTAGAGCCCACATCTCCCACCTTC |
| mm4_intergenic_Dmrta2|Elavl4_chr4_110118310 |
CTGGACCTGAAACTGACCCCTAATTTCTGGGCTTTGGAGCTAAGTTGGGTTCTGAGAGTTTCCAGTTATTCTTCTCATCT TTTGACACTCACTCTCCTAATCTGTCTTCATCATCCAGCA |
| mm4_intron_Pqlc2_chr4_139300629 |
GCTTGGCACTCAAAACTCCCTGTCCATGGTCAGCTCCAAATCCCAGCTCCAAGCACTGCCCCCAGGACCATGGGTAGCCC TGGCTGTGGCCCCCACGGACTCCTTCTGCCAGGTAAGCCTCCAGTAGGGGTCC |
| mm4_intron_Cpa5_chr6_30626576 |
AAAGCCGTCTTGCCCAGTAACCTGCCCACTGCCCAAGGAAGCTTCTACTACCAGCGTATGGTTAGATGTCTTGCCATGCT TACTTGAACCACAGATCTAATCTGCTCACACGCCCAGTTCCATGTCAGCCCAT |
| mm4_intergenic_Gm10617|Gm25200_chr8_117950289 |
CACCTTGGCTCTTACTGGCATGACTTTGGCTTCCCAAGGAAACGCCTAAACCCAGAAGCTAGCAGCCATGCAGGGCTCTG CTCAGGTCTGTGTT |
| mm4_intergenic_Gm23345|Usp47_chr7_111950232 |
CAGAGACATAGACTTCAAGGCAGTCCCTGAGTAAGCACCAAATCCCAATGGGTCTTACGTTAGCATGGCTATATTTTTAT TTCCATCAGATTATGAGTCATCCAAGATCCAAAAACCCCTCCACCACTGTG |
| mm3_intron_Auts2_chr5_131707748 |
GTTTTGGCAAGCGGTCTGGTGTTCCCCTACCAAGCTCCTAATCCCAGCAGATCAGTTAAAATAAATGAAAGAGAGAATCA AAAGGCACTTAAAAAATTCAACTGTTCTCCAAATGAGTTTGAACAGGCTCCAGA |
| mm4_intergenic_5730522E02Rik|mmu-mir-6372_chr11_26159264 |
AGAGTTGGGTAGGGAGCTGTATCCAGGGCAACTCAGAACCAAGTTAAAGCAGGGACACTGCTTGTAGAGCAGGGTTGACA CTGGGATTAGGAGCTGTTCTAGGAGACCGTGCCAGCCTGATAAAT |
| mm4_intron_Myom3_chr4_135783502 |
AGGTCTCTGCTGAGCCTCTTAAATGGGTGGACTTCCTACGCAGGCTCCTACTCCCAGTCCGTGTGAGAACTGACTCTCCA GGCAGACCGAGTGTCGTCTCTTGCCAAGCTC |
| mm4_intergenic_mmu-mir-6238|AC161571.1_chr7_53920560 |
AGAGTTTGGCTACTTGTAAAACTGAATTGTACAAATGAAGAAAGTTACCTGGGTTTTGGAGCTTAGTCAGGAGATTCAAA ACATGTTTTCAGCTTTTCTCACCAATGGT |
| mm4_intergenic_Gm25329|mmu-mir-6351_chr1_73826931 |
GGCTGACGGAAACACATCCTGCCTGAGTACGCTCCTCATCTCAGGGATGAGCCCAAGGCCCTTGAGCTTCCAGAAGCATT GAGCATCGGC |
| mm4_intron_Lhx6_chr2_36094177 |
GACCACGAACTTGGGTGCTAGGGAACCTGGGAAAGGACTGGGATTAGGTGCTGGGTTGGGCGGATTGGGTACCGTTCTCG GCGGCCCTCTTGAGGTTCTCGATCATGGTGTCGTAATGGATGCGGCAA |
| mm4_intron_St18_chr1_6829809 |
AAGTTCTCCCAGTGCCATCCTGCGTATGCAGAAGAACCAAAGTAACACCCTACTCCCAGGAGCTGACGAGGCAGCAGGCA GGGTGCTGGGAGCTGTTGTGGGCAGAAT |
| mm4_intergenic_Rarb|Thrb_chr14_16797503 |
TTCTGCTCTGGTCTGTCTGCCCCTACCCCTTCTCCATGCCCCTACTTCCTTTTTGCCCAAATAACCTCCTTATACCAGGT GTATTGCATGTCGTGATTTCCCAGTCCC |