CRISPOR

DNA break repair outcome predictions

Guide: GGGACCCCCAGTCCCCTCCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGA`	(Wild-type sequence)
365	`TGCTTGGCCCGGGACCCCCAGTCCCCTC-------------TCTGGCCAAGGGCAACCTA`	`13 bp deleted → frameshift`
362	`TGCTTGGCCCGGGACCCCCAGTCCCCTCCG--GACAGCCTCTCTGGCCAAGGGCAACCTA`	`2 bp deleted → frameshift`
257	`TGCTTGGCCCGGGAC--------------------AGCCTCTCTGGCCAAGGGCAACCTA`	`20 bp deleted → frameshift`
242	`TGCTTGGCCCGGGACCCCCAGTCCCCTCC----------TCTCTGGCCAAGGGCAACCTA`	`10 bp deleted → frameshift`
235	`TGCTTGGCCCGGGACCCCCAGTCCCCTC---------------TGGCCAAGGGCAACCTA`	`15 bp deleted → no frameshift`
224	`TGCTTGGCCCGGGACCCCCAG----------------CCTCTCTGGCCAAGGGCAACCTA`	`16 bp deleted → frameshift`
210	`TGCTTGGCCCGG---------------------ACAGCCTCTCTGGCCAAGGGCAACCTA`	`21 bp deleted → no frameshift`
198	`TGCTTGGCCCGGGACCCCCAGTCCC--------------TCTCTGGCCAAGGGCAACCTA`	`14 bp deleted → frameshift`
188	`TGCTTGGCCCGGGACCCCCAGTCC---------------TCTCTGGCCAAGGGCAACCTA`	`15 bp deleted → no frameshift`
154	`TGCTTGGCCCGGGACCCCCAGTCCCCTCC-------------------AAGGGCAACCTA`	`19 bp deleted → frameshift`
147	`TGCTTGGCCCGGGACCCCC--------------------TCTCTGGCCAAGGGCAACCTA`	`20 bp deleted → frameshift`
140	`TGCTTGGCCCGGGACCCC---------------------TCTCTGGCCAAGGGCAACCTA`	`21 bp deleted → no frameshift`
133	`TGCTTGGCC------------------------------TCTCTGGCCAAGGGCAACCTA`	`30 bp deleted → no frameshift`
133	`TGCTTGGCCCGGGACCCCCAGTCCCC----------------------AAGGGCAACCTA`	`22 bp deleted → frameshift`
133	`TGCTTGGCCCGGGACCC----------------------TCTCTGGCCAAGGGCAACCTA`	`22 bp deleted → frameshift`
128	`TGCTTGGCCCGGGACCCCCAGTCCCCT-----------------GGCCAAGGGCAACCTA`	`17 bp deleted → frameshift`
127	`TGCTTGGCC---------------------------------------AAGGGCAACCTA`	`39 bp deleted → no frameshift`
126	`TGCTTGGCCCGGGACCCCCAGTCCC-----------------------AAGGGCAACCTA`	`23 bp deleted → frameshift`
126	`TGCTTGGCCCGGGACC-----------------------TCTCTGGCCAAGGGCAACCTA`	`23 bp deleted → frameshift`
122	`TGCTTGGCCCGGGACCCCCAGTC------------------TCTGGCCAAGGGCAACCTA`	`18 bp deleted → no frameshift`
120	`TGCTTGGCCCGGGACCCCCAGTCC------------------------AAGGGCAACCTA`	`24 bp deleted → no frameshift`
117	`TGCTTGGCCCGGGACCCCCA-----------------------------AGGGCAACCTA`	`29 bp deleted → frameshift`
110	`TGCTTGGCCCGGGACCCCCAGTC--------------------TGGCCAAGGGCAACCTA`	`20 bp deleted → frameshift`
109	`TGCTTGG--------------------------ACAGCCTCTCTGGCCAAGGGCAACCTA`	`26 bp deleted → frameshift`
101	`TGCTTGGCCCGGGACCCCCAGTCCCCT--------------------------------A`	`32 bp deleted → frameshift`
94	`TGCTTGGCCCGGGACCCCCAGTCCCCTCC-----------------------------TA`	`29 bp deleted → frameshift`
94	`TGCTTGGCCC-----------------------------TCTCTGGCCAAGGGCAACCTA`	`29 bp deleted → frameshift`
89	`TGCTTGGCCCGGGACCCC------------------------------AAGGGCAACCTA`	`30 bp deleted → no frameshift`
84	`TGCTTGGCCCGGGACCC-------------------------------AAGGGCAACCTA`	`31 bp deleted → frameshift`
81	`TGCTTGGCCCGGG----------------------------------------CAACCTA`	`40 bp deleted → frameshift`
80	`TGCTTGGCCCGGGACC--------------------------------AAGGGCAACCTA`	`32 bp deleted → frameshift`
76	`TGCTTGGCCCGGGACCCCCAGTCCC---------------------------------TA`	`33 bp deleted → no frameshift`
76	`TGCTTGGCCCGGG---------------------------------CCAAGGGCAACCTA`	`33 bp deleted → no frameshift`
73	`TGCTTGGCCCGGGACCCCCAGTCC----------------------------------TA`	`34 bp deleted → frameshift`
73	`TGCTTGGCCCGG----------------------------------CCAAGGGCAACCTA`	`34 bp deleted → frameshift`
69	`TGC-----------------------------------CTCTCTGGCCAAGGGCAACCTA`	`35 bp deleted → frameshift`
66	`TGCTTGGCCCGGGACCCCCAG------------------------------GGCAACCTA`	`30 bp deleted → no frameshift`
61	`TGCTTGGCCCGGGACC------------------------------------------TA`	`42 bp deleted → no frameshift`
60	`TGCTTGGCCC--------------------------------------AAGGGCAACCTA`	`38 bp deleted → frameshift`
60	`TGCTTGGC----------------------------------------------AACCTA`	`46 bp deleted → frameshift`
56	`TGCTTGGCCCGGGACCCCC---------------------------------------TA`	`39 bp deleted → no frameshift`
56	`TGCTTGGCCCGGG---------------------------------------GCAACCTA`	`39 bp deleted → no frameshift`
54	`TGCTTGGCCCGGGACCCC----------------------------------------TA`	`40 bp deleted → frameshift`
52	`TGCTTGGCCCGGGACCCCCA-----------------------------------ACCTA`	`35 bp deleted → frameshift`
51	`TGCTTGGCCCGGGACCC-----------------------------------------TA`	`41 bp deleted → frameshift`
51	`TGCTTGGCCCGG-----------------------------------------CAACCTA`	`41 bp deleted → frameshift`
49	`TGCT------------------------------------CTCTGGCCAAGGGCAACCTA`	`36 bp deleted → no frameshift`
45	`TGCT--------------------------------------CTGGCCAAGGGCAACCTA`	`38 bp deleted → frameshift`
44	`TGC--------------------------------------------CAAGGGCAACCTA`	`44 bp deleted → frameshift`
42	`TGCTTGG---------------------------------------------GCAACCTA`	`45 bp deleted → no frameshift`
40	`TGCT----------------------------------------GGCCAAGGGCAACCTA`	`40 bp deleted → frameshift`
36	`TGCTTGGCCC------------------------------------------------TA`	`48 bp deleted → no frameshift`
34	`TG-------------------------------------------GCCAAGGGCAACCTA`	`43 bp deleted → frameshift`
34	`TGCTTGGCC-------------------------------------------------TA`	`49 bp deleted → frameshift`
31	`TGC---------------------------------------------------AACCTA`	`51 bp deleted → no frameshift`
19	`TGCT-------------------------------------------------------A`	`55 bp deleted → frameshift`

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