CRISPOR

DNA break repair outcome predictions

Guide: CTGGGATTAGGAGCTTACTT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGAT`	(Wild-type sequence)
244	`CCCAGGGAGCCTGGG------------------GGTTTTCCCCCTCCCCCCCTCTGGAGA`	`18 bp deleted → no frameshift`
232	`CCCAGGGAGCCTGGG-------------------GTTTTCCCCCTCCCCCCCTCTGGAGA`	`19 bp deleted → frameshift`
230	`CCCAGGGAGCCTGGGATTAGG-----------GGGTTTTCCCCCTCCCCCCCTCTGGAGA`	`11 bp deleted → frameshift`
220	`CCCAGGGAGCCTGGG--------------------TTTTCCCCCTCCCCCCCTCTGGAGA`	`20 bp deleted → frameshift`
219	`CCCAGGGAGCCTGGGATTAGG------------GGTTTTCCCCCTCCCCCCCTCTGGAGA`	`12 bp deleted → no frameshift`
208	`CCCAGGGAGCCTGGGATTAGG-------------GTTTTCCCCCTCCCCCCCTCTGGAGA`	`13 bp deleted → frameshift`
198	`CCCAGGGAGCCTGGGATTAGG--------------TTTTCCCCCTCCCCCCCTCTGGAGA`	`14 bp deleted → frameshift`
170	`CCCAGGGAGCCTGGG-----------------GGGTTTTCCCCCTCCCCCCCTCTGGAGA`	`17 bp deleted → frameshift`
163	`CCCAGGG--------------------------GGTTTTCCCCCTCCCCCCCTCTGGAGA`	`26 bp deleted → frameshift`
155	`CCCAGGG---------------------------GTTTTCCCCCTCCCCCCCTCTGGAGA`	`27 bp deleted → no frameshift`
148	`CCCAGGG----------------------------TTTTCCCCCTCCCCCCCTCTGGAGA`	`28 bp deleted → frameshift`
141	`CCCAGGGAGCCTGGGATTAGGAGCTTACTT-------TTCCCCCTCCCCCCCTCTGGAGA`	`7 bp deleted → frameshift`
140	`CCCAGGGAGCCTGG---------------------TTTTCCCCCTCCCCCCCTCTGGAGA`	`21 bp deleted → no frameshift`
134	`CCCAGGGAGCCTGGGATTAGGAGCTTACT----------------CCCCCCCTCTGGAGA`	`16 bp deleted → frameshift`
134	`CCCAGGGAGCCTGGGATTAGGAGCTTACTT--------TCCCCCTCCCCCCCTCTGGAGA`	`8 bp deleted → frameshift`
127	`CCCAGGGAGCCTGGGATTAGGAGCTTACTT---------CCCCCTCCCCCCCTCTGGAGA`	`9 bp deleted → no frameshift`
115	`CCCAGGGAGCCTGGGATTAGGAG------------------------------------A`	`36 bp deleted → no frameshift`
115	`CCCAGGGAGCCTGGGATTAGGAGCTT-----------TTCCCCCTCCCCCCCTCTGGAGA`	`11 bp deleted → frameshift`
114	`CCCAGGG-------------------------GGGTTTTCCCCCTCCCCCCCTCTGGAGA`	`25 bp deleted → frameshift`
110	`CCCAGGGAGCCTGGGATTAGGAGCT--------------------CCCCCCCTCTGGAGA`	`20 bp deleted → frameshift`
109	`CCCAGGGAGCCTGGGATTAGGAGCTT------------TCCCCCTCCCCCCCTCTGGAGA`	`12 bp deleted → no frameshift`
104	`CCCAGGGAGCCTGGGATTAGGAGCTT-------------CCCCCTCCCCCCCTCTGGAGA`	`13 bp deleted → frameshift`
96	`CCCAGGGAGCCT---------------------------------CCCCCCCTCTGGAGA`	`33 bp deleted → no frameshift`
94	`CCCAGG-----------------------------TTTTCCCCCTCCCCCCCTCTGGAGA`	`29 bp deleted → frameshift`
90	`CCCAGGGAGCCTGGGATTAGGAGCTTACT------------------------CTGGAGA`	`24 bp deleted → no frameshift`
89	`CCCAGGGAGCC------------------------------CCCTCCCCCCCTCTGGAGA`	`30 bp deleted → no frameshift`
85	`CCC---------------------------------------CCTCCCCCCCTCTGGAGA`	`39 bp deleted → no frameshift`
84	`CCCAGGGAGCC-------------------------------CCTCCCCCCCTCTGGAGA`	`31 bp deleted → frameshift`
81	`CCCAGGGAGCCTGGGATTAGGAGCTTACT--------------------------GGAGA`	`26 bp deleted → frameshift`
81	`CCCAGGGAGCCTGG-------------------------------------------AGA`	`43 bp deleted → frameshift`
81	`CCC----------------------------------------CTCCCCCCCTCTGGAGA`	`40 bp deleted → frameshift`
80	`CCCAGGGAGCC--------------------------------CTCCCCCCCTCTGGAGA`	`32 bp deleted → frameshift`
77	`CCCAGGGAGCCTGGGATT-------------------TTCCCCCTCCCCCCCTCTGGAGA`	`19 bp deleted → frameshift`
77	`CCC-----------------------------------------TCCCCCCCTCTGGAGA`	`41 bp deleted → frameshift`
74	`CCCAGGGAGCCTGGGATTAGGAGCT----------------------------CTGGAGA`	`28 bp deleted → frameshift`
73	`CCCAGGGAGCCTGGGATT--------------------TCCCCCTCCCCCCCTCTGGAGA`	`20 bp deleted → frameshift`
70	`CCCAGGGAGCCTGGGATT---------------------CCCCCTCCCCCCCTCTGGAGA`	`21 bp deleted → no frameshift`
66	`CCCAGGGAGCCTGGGATTAGGAGCT------------------------------GGAGA`	`30 bp deleted → no frameshift`
66	`CCCAGGGAGCC------------------------------------CCCCCTCTGGAGA`	`36 bp deleted → no frameshift`
64	`CCCAGGGAGCCT-----------------------------------------CTGGAGA`	`41 bp deleted → frameshift`
63	`CCC---------------------------------------------CCCCTCTGGAGA`	`45 bp deleted → no frameshift`
62	`CCCAGGGAGCC-------------------------------------CCCCTCTGGAGA`	`37 bp deleted → frameshift`
61	`CCCAGGGAGCCTGGGA------------------------------------------GA`	`42 bp deleted → no frameshift`
60	`CCCAGGGAGCC--------------------------------------CCCTCTGGAGA`	`38 bp deleted → frameshift`
60	`CCC--------------------------------------CCCTCCCCCCCTCTGGAGA`	`38 bp deleted → frameshift`
60	`CCC----------------------------------------------CCCTCTGGAGA`	`46 bp deleted → frameshift`
57	`CCCAGGGAG--------------------------------------------------A`	`50 bp deleted → frameshift`
57	`CCC-----------------------------------------------CCTCTGGAGA`	`47 bp deleted → frameshift`
56	`CCCAGGGAGCC---------------------------------------CCTCTGGAGA`	`39 bp deleted → no frameshift`
54	`CCC------------------------------------------------CTCTGGAGA`	`48 bp deleted → no frameshift`
54	`CCCAGGGAGCC----------------------------------------CTCTGGAGA`	`40 bp deleted → frameshift`
51	`CCC-------------------------------------------------TCTGGAGA`	`49 bp deleted → frameshift`
48	`CC------------------------------------------TCCCCCCCTCTGGAGA`	`42 bp deleted → no frameshift`
45	`CCCAGGGAGCCTGGGATTAGGA--------------------------------------`	`38 bp deleted → frameshift`
44	`CCC--------------------------------------------CCCCCTCTGGAGA`	`44 bp deleted → frameshift`
42	`CCCAGGGAGCCTGGGATTAG---------------------------------------A`	`39 bp deleted → no frameshift`
33	`CCCAGGGAGCCTGGGA--------------------------------------------`	`44 bp deleted → frameshift`
32	`CC--------------------------------------------------TCTGGAGA`	`50 bp deleted → frameshift`
31	`CCCAGG---------------------------------------------------AGA`	`51 bp deleted → no frameshift`
22	`CCCAGGGA----------------------------------------------------`	`52 bp deleted → frameshift`
20	`CCCAG------------------------------------------------------A`	`54 bp deleted → no frameshift`

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