CRISPOR

DNA break repair outcome predictions

Guide: CCCTCTGGAGAGTCCGGGGA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGG`	(Wild-type sequence)
519	`CCCCCTCCCCCCCTCTGGAGAG---------------CCGAAAGGACATGGTTATGTTTC`	`15 bp deleted → no frameshift`
409	`CCCCCTCCCCCCCTCTGGAGAGTCCGGGGA----GAGCCGAAAGGACATGGTTATGTTTC`	`4 bp deleted → frameshift`
311	`CCCCCTCCCCCCCTCTGGAGAGTCCGGG-----AGAGCCGAAAGGACATGGTTATGTTTC`	`5 bp deleted → frameshift`
298	`CCCCCTCCCCCCCTCTGGAGAGTCCG--------------AAAGGACATGGTTATGTTTC`	`14 bp deleted → frameshift`
296	`CCCCCTCCCCCCCTCTGGAGAGTCCGG------AGAGCCGAAAGGACATGGTTATGTTTC`	`6 bp deleted → no frameshift`
261	`CCCCCTCCCCCCCTCTGGAGAG-------------AGCCGAAAGGACATGGTTATGTTTC`	`13 bp deleted → frameshift`
224	`CCCCCTCCCCCCCTCTGGAGAGTCCGGGGA----------------CATGGTTATGTTTC`	`16 bp deleted → frameshift`
222	`CCCCCTCCCCCCCTCTGGAGAGTCCGGGGA------GCCGAAAGGACATGGTTATGTTTC`	`6 bp deleted → no frameshift`
213	`CCCCCTCCCCCCCTCTGGAG-----------------CCGAAAGGACATGGTTATGTTTC`	`17 bp deleted → frameshift`
173	`CCCCCTCCCCCCCTCTGGAGAGTCCGGGGA-----------AAGGACATGGTTATGTTTC`	`11 bp deleted → frameshift`
170	`CCCCCTCCCCCCCTCTGGAGAGTCCGGG-----------------ACATGGTTATGTTTC`	`17 bp deleted → frameshift`
162	`CCCCCTCCCCCCCTCTGGAGAGTCCGG------------------ACATGGTTATGTTTC`	`18 bp deleted → no frameshift`
133	`CCCCCTCCCCCCCTCTGGAGAGTCCGGGG----------------------TTATGTTTC`	`22 bp deleted → frameshift`
129	`CCCCCTCCCCCCCTCTGGA---------------------------CATGGTTATGTTTC`	`27 bp deleted → no frameshift`
126	`CCCCCTCCCCCCCTCTGGAGAGTCCGGG-----------------------TTATGTTTC`	`23 bp deleted → frameshift`
120	`CCCCCTCCCCCCCTCTGGAGAGTCCGG------------------------TTATGTTTC`	`24 bp deleted → no frameshift`
110	`CCCCCTCCCCCCCTCTGGAGA--------------------AAGGACATGGTTATGTTTC`	`20 bp deleted → frameshift`
109	`CCCCCTCCCCCCC--------------------------GAAAGGACATGGTTATGTTTC`	`26 bp deleted → frameshift`
103	`CCCCCTCCCCCC---------------------------GAAAGGACATGGTTATGTTTC`	`27 bp deleted → no frameshift`
99	`CCCCCTCCCCCCCTCTGGAGAG----------------------GACATGGTTATGTTTC`	`22 bp deleted → frameshift`
99	`CCCCCTCCCCCCCTCTGGA----------------------AAGGACATGGTTATGTTTC`	`22 bp deleted → frameshift`
98	`CCCCCTCCCCC----------------------------GAAAGGACATGGTTATGTTTC`	`28 bp deleted → frameshift`
96	`CCCCCTCCCCCCCTCTGG---------------------------------TTATGTTTC`	`33 bp deleted → no frameshift`
94	`CCCCCTCCCC-----------------------------GAAAGGACATGGTTATGTTTC`	`29 bp deleted → frameshift`
90	`CCCCCTCCCCCCCTCTGGAG------------------------GACATGGTTATGTTTC`	`24 bp deleted → no frameshift`
89	`CCCCCTCCC------------------------------GAAAGGACATGGTTATGTTTC`	`30 bp deleted → no frameshift`
86	`CCCCCTCCCCCCCTCTGGAGA-------------------------CATGGTTATGTTTC`	`25 bp deleted → frameshift`
84	`CCCCCTCC-------------------------------GAAAGGACATGGTTATGTTTC`	`31 bp deleted → frameshift`
73	`CCCCC----------------------------------GAAAGGACATGGTTATGTTTC`	`34 bp deleted → frameshift`
70	`CCCCCTCCCCCCCTCTGGAGAGT-----------------------------TATGTTTC`	`29 bp deleted → frameshift`
69	`CCCC-----------------------------------GAAAGGACATGGTTATGTTTC`	`35 bp deleted → frameshift`
66	`CCC------------------------------------GAAAGGACATGGTTATGTTTC`	`36 bp deleted → no frameshift`
62	`CC-------------------------------------GAAAGGACATGGTTATGTTTC`	`37 bp deleted → frameshift`
54	`CCCCCTCCCCCCCTCTGGAGAGT----------------------------------TTC`	`34 bp deleted → frameshift`
49	`CCCCCTCCCCCCCTCTGGAGAGTC------------------------------------`	`36 bp deleted → no frameshift`
42	`CCCCCTCCCCCCCTCTG---------------------------------------TTTC`	`39 bp deleted → no frameshift`
31	`CCCCCTCCCCCCCTC---------------------------------------------`	`45 bp deleted → no frameshift`
21	`CCCCCTC-----------------------------------------------------`	`53 bp deleted → frameshift`

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