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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313979_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313979_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4_intergenic_Olfr420|Spta1_chr1_174171647_F | Primer3: not found at this Tm | N.d. | 0.62 |
| mm4_intergenic_Olfr420|Spta1_chr1_174171647_R | Primer3: not found at this Tm | N.d. | 0.62 |
| mm4_intron_Adamtsl2_chr2_27088654_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intron_Adamtsl2_chr2_27088654_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Sema6a|Gm5506_chr18_47510242_F | TCGTCGGCAGCGTCATTGTATCTGCCCAGCCCTG | 59.8 | 0.31 |
| mm4_intergenic_Sema6a|Gm5506_chr18_47510242_R | GTCTCGTGGGCTCGGATAAGCCTGGGAGGTGACCT | 59.9 | 0.31 |
| mm4_intron_Ift122_chr6_115855154_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Ift122_chr6_115855154_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm19965|Gm24030_chr1_117520101_F | TCGTCGGCAGCGTCCAACTGTGGATCCTCAGCTGT | 59.9 | 0.28 |
| mm4_intergenic_Gm19965|Gm24030_chr1_117520101_R | GTCTCGTGGGCTCGGGGGATGTAAGGTGGTGCCAA | 59.9 | 0.28 |
| mm4_intergenic_Otop2|Otop3_chr11_115332962_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Otop2|Otop3_chr11_115332962_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Mrgprx1|Mrgprb5_chr7_48084695_F | TCGTCGGCAGCGTCTCACAGCAAGGAAAGAGGGG | 59.5 | 0.20 |
| mm4_intergenic_Mrgprx1|Mrgprb5_chr7_48084695_R | GTCTCGTGGGCTCGGTCTTTCCAGTGGTGTGCCTC | 59.8 | 0.20 |
| mm4_intron_Arhgap24_chr5_102515443_F | TCGTCGGCAGCGTCCAGGGTCAGTTCATTCGCCA | 60.3 | 0.17 |
| mm4_intron_Arhgap24_chr5_102515443_R | GTCTCGTGGGCTCGGTGAAGATCGGGGAAGAAAGCC | 60.0 | 0.17 |
| mm4_intron_Coro2a_chr4_46589522_F | TCGTCGGCAGCGTCAGACCCAAAATGACAGCCCT | 59.2 | 0.15 |
| mm4_intron_Coro2a_chr4_46589522_R | GTCTCGTGGGCTCGGAGAGGGCTCACAGAGTGGAT | 59.9 | 0.15 |
| mm4_intergenic_Agap1|Gm25180_chr1_89496065_F | TCGTCGGCAGCGTCGTCTACTTAGGGCACGGGTG | 59.8 | 0.15 |
| mm4_intergenic_Agap1|Gm25180_chr1_89496065_R | GTCTCGTGGGCTCGGACAGTGGGCTGGAGAAAACC | 60.1 | 0.15 |
| mm4_intergenic_Nfasc|Gm22609_chr1_132813549_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Nfasc|Gm22609_chr1_132813549_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Epas1|Tmem247_chr17_86833573_F | TCGTCGGCAGCGTCACCCCACAGGAAGTCCAATG | 59.5 | 0.13 |
| mm3_intergenic_Epas1|Tmem247_chr17_86833573_R | GTCTCGTGGGCTCGGAAAGGGCCTTGGCTAGACAC | 59.9 | 0.13 |
| mm3_intergenic_Smap2|Col9a2_chr4_121024897_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Smap2|Col9a2_chr4_121024897_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_1700019O17Rik|Ptma_chr1_86522042_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_1700019O17Rik|Ptma_chr1_86522042_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Scn11a|Gm2449_chr9_119850024_F | TCGTCGGCAGCGTCGACGAGCTCTGGATGGTTCC | 60.1 | 0.11 |
| mm4_intergenic_Scn11a|Gm2449_chr9_119850024_R | GTCTCGTGGGCTCGGGGATGAGCCTGGTTTCCCAA | 59.9 | 0.11 |
| mm4_intergenic_Fignl2|Ankrd33_chr15_101077898_F | TCGTCGGCAGCGTCTGGACAAAGACCTGACGGAG | 59.3 | 0.10 |
| mm4_intergenic_Fignl2|Ankrd33_chr15_101077898_R | GTCTCGTGGGCTCGGGGAGCTAGGATTTGCGGGTT | 60.1 | 0.10 |
| mm4_exon_Rgl3_chr9_21988157_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Rgl3_chr9_21988157_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Begain_chr12_109034164_F | TCGTCGGCAGCGTCGATGTCTCCCTTGTACGGGG | 59.5 | 0.09 |
| mm4_exon_Begain_chr12_109034164_R | GTCTCGTGGGCTCGGATCGCCAAAGTGCTGGAGAA | 59.9 | 0.09 |
| mm4_intron_Smpd4_chr16_17633076_F | TCGTCGGCAGCGTCTGCAGCCAGAGTTACTCACG | 60.0 | 0.08 |
| mm4_intron_Smpd4_chr16_17633076_R | GTCTCGTGGGCTCGGGCTGCAGCAAAGCAAGCTT | 60.3 | 0.08 |
| mm4_intergenic_Gm24839|Tmem132c_chr5_126688202_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm24839|Tmem132c_chr5_126688202_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Unc13a_chr8_71636248_F | TCGTCGGCAGCGTCAAAAGTCACAGTCTGGCCCC | 60.1 | 0.07 |
| mm4_intron_Unc13a_chr8_71636248_R | GTCTCGTGGGCTCGGGCAACAAGGGAAACTGAGGC | 59.6 | 0.07 |
| mm4_intron_Ranbp3_chr17_56673759_F | TCGTCGGCAGCGTCCCCAACGGGCCTTGAGAC | 60.3 | 0.07 |
| mm4_intron_Ranbp3_chr17_56673759_R | GTCTCGTGGGCTCGGGCTATGCCGGGGAAGGAG | 59.5 | 0.07 |
| mm4_intron_Maml3_chr3_51790595_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Maml3_chr3_51790595_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Dock6|Rab3d_chr9_21878048_F | TCGTCGGCAGCGTCCCTCGGTCCACCTCTTTCAC | 60.0 | 0.07 |
| mm4_intergenic_Dock6|Rab3d_chr9_21878048_R | GTCTCGTGGGCTCGGCACTGGCAAGGCTAAGCTCT | 60.0 | 0.07 |
| mm4_exon_Frmd5_chr2_121806675_F | TCGTCGGCAGCGTCCGGGGTTAGAGCTGGAGC | 59.4 | 0.06 |
| mm4_exon_Frmd5_chr2_121806675_R | GTCTCGTGGGCTCGGCGAGTACACCTGCACCATCC | 60.4 | 0.06 |
| mm4_intron_Neurl1b_chr17_26415086_F | TCGTCGGCAGCGTCGGGTGCAAGCCAGAGCCT | 62.3 | 0.06 |
| mm4_intron_Neurl1b_chr17_26415086_R | GTCTCGTGGGCTCGGCAGGAAAACCAGGAGGGGTC | 59.9 | 0.06 |
| mm4_intron_Rgs3_chr4_62643372_F | TCGTCGGCAGCGTCGAACTCTGTAGGGAGCGTGG | 59.8 | 0.06 |
| mm4_intron_Rgs3_chr4_62643372_R | GTCTCGTGGGCTCGGGAGCTCTGGAGGAGGAGACA | 60.0 | 0.06 |
| mm4_intron_Sorbs2_chr8_45788350_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sorbs2_chr8_45788350_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Slc39a14|Gm9174_chr14_70360140_F | TCGTCGGCAGCGTCTCCTGACATTTCACCAGCCC | 59.9 | 0.05 |
| mm4_intergenic_Slc39a14|Gm9174_chr14_70360140_R | GTCTCGTGGGCTCGGACTCCTCAATGTCGTCGAGC | 59.8 | 0.05 |
| mm4_exon_Cdc26_chr4_62402827_F | TCGTCGGCAGCGTCACTCCTCAATGTCGTCGAGC | 59.8 | 0.05 |
| mm4_exon_Cdc26_chr4_62402827_R | GTCTCGTGGGCTCGGCAGCTTTTCCTCTCCTGCCA | 59.9 | 0.05 |
| mm4_intron_Ntrk2_chr13_58922445_F | TCGTCGGCAGCGTCATTTCAGGTCTAGGCGAGCG | 59.8 | 0.05 |
| mm4_intron_Ntrk2_chr13_58922445_R | GTCTCGTGGGCTCGGAAAGTCACCGTTCTAGCCCC | 59.6 | 0.05 |
| mm4_intergenic_RP23-341H6.1|Lca5_chr9_83257237_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_RP23-341H6.1|Lca5_chr9_83257237_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm12450|Spag17_chr3_99834837_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm12450|Spag17_chr3_99834837_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Zfp521|Gm5160_chr18_14331862_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Zfp521|Gm5160_chr18_14331862_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ppp1r16b|Gm26141_chr2_158701102_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ppp1r16b|Gm26141_chr2_158701102_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22729|Oxsr1_chr9_119319358_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22729|Oxsr1_chr9_119319358_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_B3galt1_chr2_67897373_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_B3galt1_chr2_67897373_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Pcdh15_chr10_74547627_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Pcdh15_chr10_74547627_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm13018|Prdm2_chr4_142891650_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm13018|Prdm2_chr4_142891650_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Atxn7l3|Ubtf_chr11_102297244_F | TCGTCGGCAGCGTCCAATAGGCTCGAGGTGGGTG | 60.1 | 0.03 |
| mm4_intergenic_Atxn7l3|Ubtf_chr11_102297244_R | GTCTCGTGGGCTCGGCTCTCTTTCGACGCCACCC | 60.4 | 0.03 |
| mm4_intergenic_Cbll1|Slc26a4_chr12_31503988_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Cbll1|Slc26a4_chr12_31503988_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_A630031M04Rik|1700112K13Rik_chr4_127675919_F | TCGTCGGCAGCGTCTCAGACCTCTTGTGCCCTCT | 60.1 | 0.03 |
| mm4_intergenic_A630031M04Rik|1700112K13Rik_chr4_127675919_R | GTCTCGTGGGCTCGGGCTTGACGTCCTATCCCCAG | 59.8 | 0.03 |
| mm3_intergenic_Chst1|Gm13816_chr2_92718806_F | TCGTCGGCAGCGTCACCCAGCTCTCTGTCTGTCT | 59.8 | 0.03 |
| mm3_intergenic_Chst1|Gm13816_chr2_92718806_R | GTCTCGTGGGCTCGGAATGTCTGTGCTGGGACCAG | 59.9 | 0.03 |
| mm4_exon_Hagh_chr17_24850965_F | TCGTCGGCAGCGTCAGTCCGACTCCAGTTTTGGG | 59.6 | 0.02 |
| mm4_exon_Hagh_chr17_24850965_R | GTCTCGTGGGCTCGGCAAAGTTCCCGGCTAGCTGA | 60.0 | 0.02 |
| mm4_intron_Cped1_chr6_22156828_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cped1_chr6_22156828_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Coro1a|Mapk3_chr7_126721806_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Coro1a|Mapk3_chr7_126721806_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam171a1_chr2_3197636_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam171a1_chr2_3197636_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Cdrt4|4930452L02Rik_chr11_62995474_F | TCGTCGGCAGCGTCCCCAATGAGAAGAGGCTGCA | 60.0 | 0.01 |
| mm4_intergenic_Cdrt4|4930452L02Rik_chr11_62995474_R | GTCTCGTGGGCTCGGTATCCGCTCAGGTCCCATCT | 59.8 | 0.01 |
| mm4_intergenic_Bard1|Abca12_chr1_71200209_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Bard1|Abca12_chr1_71200209_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Acan_chr7_79062900_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Acan_chr7_79062900_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Grb2_chr11_115708052_F | TCGTCGGCAGCGTCCAATCACCACGTTAGCTGCG | 59.9 | 0.00 |
| mm4_intron_Grb2_chr11_115708052_R | GTCTCGTGGGCTCGGCAGGCTGCTGCTCTGAGTC | 60.4 | 0.00 |
| mm4_intergenic_BC034090|Xpr1_chr1_155258396_F | TCGTCGGCAGCGTCTGAGAATGGTGGCAAATCAAAGG | 59.7 | 0.00 |
| mm4_intergenic_BC034090|Xpr1_chr1_155258396_R | GTCTCGTGGGCTCGGCCAGAGTTTGGTACCTCACTCT | 59.0 | 0.00 |
| mm4_intron_Slit3_chr11_35121723_F | TCGTCGGCAGCGTCCAGAGCGCCTGTGAGTACC | 60.1 | 0.00 |
| mm4_intron_Slit3_chr11_35121723_R | GTCTCGTGGGCTCGGGGGGTAGGGGAGTAGGGATC | 59.8 | 0.00 |
| mm4_intron_Rab6b_chr9_103113614_F | TCGTCGGCAGCGTCCCACCTCCTGTCTGCTGATG | 60.1 | 0.00 |
| mm4_intron_Rab6b_chr9_103113614_R | GTCTCGTGGGCTCGGTCAGATCCAGCTGGCTACCT | 60.0 | 0.00 |
| mm4_intron_Nfil3_chr13_52978466_F | TCGTCGGCAGCGTCGGAGTAGAGTCAGCTTCTGGC | 59.8 | 0.00 |
| mm4_intron_Nfil3_chr13_52978466_R | GTCTCGTGGGCTCGGGAGTTCGGTGAGCTGCGC | 61.4 | 0.00 |
| mm4_intergenic_Agpat9|Gm20484_chr5_101491978_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Agpat9|Gm20484_chr5_101491978_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Sema6a|Gm5506_chr18_47510242 |
ATTGTATCTGCCCAGCCCTGATAGTACCTGCTCTCGTGCTCAGATAACCCTACAAAAAATAGAATTACATCAAGGTCACC TCCCAGGCTTAT |
| mm4_intergenic_Gm19965|Gm24030_chr1_117520101 |
CAACTGTGGATCCTCAGCTGTAAAGGCTGCATTTCAGGCAGCCATGCTCCAGGGTCCTCGGTCCTTCTCTCAGGCTCAGG GAACCCCAATTGTGTTGTGGCATTGGCACCACCTTACATCCC |
| mm4_intergenic_Mrgprx1|Mrgprb5_chr7_48084695 |
TCACAGCAAGGAAAGAGGGGGGACCCCTTGTCCTGCTTGGCCAGCTCGCAGGCTGTGGCAAACAGAGTGGGCTGAAGTCA GAGGCCCAGGGTGACAGTCTGTGGGCCTAAGACAGGCAGAGGCACACCACTGGAAAGA |
| mm4_intron_Arhgap24_chr5_102515443 |
CAGGGTCAGTTCATTCGCCACCGCAGGGCTGCATCCACCTCTGTGAGACATTCCCTTCACCAGCTCTGGGTCTCAGGCAA CTGGCTTTCTTCCCCGATCTTCA |
| mm4_intron_Coro2a_chr4_46589522 |
AGACCCAAAATGACAGCCCTTGTCCTGCTTTGGGGCTCAGGCCACCAAGTTAGTCTTCTTCCTGCACTGCAGGAGGGTCC CTGAATCTCTACCTCAGTATCCACTCTGTGAGCCCTCT |
| mm4_intergenic_Agap1|Gm25180_chr1_89496065 |
GTCTACTTAGGGCACGGGTGGATGCTGGGAGATGAAGCCTGCCTGGGCCCGCAAGCAGGGGATGGTGGCGGTTTTCTCCA GCCCACTGT |
| mm3_intergenic_Epas1|Tmem247_chr17_86833573 |
ACCCCACAGGAAGTCCAATGAAGGAAGGCGTTTGTGCCTGACAGTTGCCTGAGCCCAGGAGCTGGTCGCTATCTTCTGCA TTTCTGTAGCTACTATGTGTCTAGCCAAGGCCCTTT |
| mm4_intergenic_Scn11a|Gm2449_chr9_119850024 |
GACGAGCTCTGGATGGTTCCATACTGAAGCTGGGGATCCAGCTTGCTAGCTCAGCCAACCATGCTACCGGCTTGGGAAAC CAGGCTCATCC |
| mm4_intergenic_Fignl2|Ankrd33_chr15_101077898 |
TGGACAAAGACCTGACGGAGTGGGAGGGGCCTGCGGGCCGGCCCTCGGGCTCAGGCCGCCGGACCCTTCTGGGACCCGAA CCCGCAAATCCTAGCTCC |
| mm4_exon_Begain_chr12_109034164 |
GATGTCTCCCTTGTACGGGGGCCGCGGGCCCGACTTCTCCACTCCGTCGCGGTAGCCCAGGTCGCGGGCCGAGGCATCCG ACATGCGCGAGGACAGGCTGCCCGGGTCCGGCTTCTCCAGCACTTTGGCGAT |
| mm4_intron_Smpd4_chr16_17633076 |
TGCAGCCAGAGTTACTCACGCTCTGGGTTGGCCTGTGGAAGCCTGGGCCTGGGCTTCCCTAGGTTCCCAGAGCCCTCAAG CCGGCTCAGCCTTGTGCATGTGGCGCGGCCTTGCCGAGGCAAGCTTGCTTTGCTGCAGC |
| mm4_intron_Unc13a_chr8_71636248 |
AAAAGTCACAGTCTGGCCCCGTGAGTGCTCAGTGAGCGGCTTCTGCTGCACTACAGCCCTGTAGTCTTAGGGTTCCCTGA GGCTGTGAGCTGGAGTTACCAGAGCCTACCTGTCTGAGCCTCAGTTTCCCTTGTTGC |
| mm4_intron_Ranbp3_chr17_56673759 |
CCCAACGGGCCTTGAGACGCGGCTCCGCGGCCTCGAACGAGGCGGGCGAGACCTCCGAGCCTGCTCGCGGGCCCAGCCCG CCACGCTCCCTTCCCCGCTTCCCTCGCCTGGGGCCTGGGCCTCCTTCCCCGGCATAGC |
| mm4_intergenic_Dock6|Rab3d_chr9_21878048 | CCTCGGTCCACCTCTTTCACTTTGGTTACCTGAGCCTGGGAACGGGATGCATCACCATAGAGCTTAGCCTTGCCAGTG |
| mm4_exon_Frmd5_chr2_121806675 |
CGGGGTTAGAGCTGGAGCGACCCCGGGCGGCGGACCCCGCCAACTTGGCCGAGTCTGGGGAGCCGGATCCCNNNNNNNNN NNNNNNNNNNNNNNNNNNNCGCGGCGCTGACCTGGATGGTGCAGGTGTACTCG |
| mm4_intron_Neurl1b_chr17_26415086 |
GGGTGCAAGCCAGAGCCTGGTTGCCGGAGCCCGAGCCCCGGAGCCGCACCCCTGCAGGGATGTGCGGACCCCTCCTGGTT TTCCTG |
| mm4_intron_Rgs3_chr4_62643372 |
GAACTCTGTAGGGAGCGTGGCATGTATCAGGTGACTGTTAGGTGCTTGTTTTTGCAGACAATGAAGAACTAAAAGGAGAC CAGGGTGTTGCCTTAGCCTGGGAGCTGGGGTGTCTCCTCCTCCAGAGCTC |
| mm4_intergenic_Slc39a14|Gm9174_chr14_70360140 |
TCCTGACATTTCACCAGCCCCCAGCTCACGGGCTCTGGCTCCCCTGAAGCCCTGGGTGACTTCACTATGCTTCGACGGAA ACCGACCCGCTTGGAGCTCAAGCTCGACGACATTGAGGAGT |
| mm4_exon_Cdc26_chr4_62402827 |
ACTCCTCAATGTCGTCGAGCTTGAGCTCTAAGCGGGTCGGTTTCCGTCGCAGCATAGTGAAGTCACCCAGGACTTCAGGG GAGCCAGAGCCCGTGAGCTGGGGGCTGGTGAAATGGCAGGAGAGGAAAAGCTG |
| mm4_intron_Ntrk2_chr13_58922445 |
ATTTCAGGTCTAGGCGAGCGTTTGTCATTGCATTCTGGGAAACGAAGCTATGGATTTAGATAATCATCTGCTGCCTTAGC CCGCCAGCAGGGAACCGCAGCACTCGGGGCTAGAACGGTGACTTT |
| mm4_intergenic_Atxn7l3|Ubtf_chr11_102297244 |
CAATAGGCTCGAGGTGGGTGGGGCAGCGTTCAAGGTGAGGTGCGCGGTGCGCCAGGGGCGGAGACTTGGTGCCGTCCCGG CCGGCTCGGGGGCGGAGGCAACGGGGTGGCGTCGAAAGAGAG |
| mm4_intergenic_A630031M04Rik|1700112K13Rik_chr4_127675919 |
TCAGACCTCTTGTGCCCTCTTTAGCGTCCAGCTAGTGGCCTCAGGAAACCCCGGTAGCATCGGGCTTGTTTATGTGAAAG AGCTCCCGGACGACTGGGGATAGGACGTCAAGC |
| mm3_intergenic_Chst1|Gm13816_chr2_92718806 |
ACCCAGCTCTCTGTCTGTCTGTCTCAGGGTAGGTGGTTGCCTGAGCCCTCGAATTGGCACTGTTCTGGTCCCAGCACAGA CATT |
| mm4_exon_Hagh_chr17_24850965 |
AGTCCGACTCCAGTTTTGGGGTTGCGACTTTAGTTTTTAAGTCGGCTTTTCCTCTTAACGTCTGATGTGGCGACAGTGGG TTGCCAAAGCCCCGGAGCTGGCCAGCCTGAATTCAGCTAGCCGGGAACTTTG |
| mm4_intergenic_Cdrt4|4930452L02Rik_chr11_62995474 |
CCCAATGAGAAGAGGCTGCAAGAATCTGCAGGGAAACTTCCAGAGCAGAGGTTCTGGTAGAGGTGTATACATTTGGGGGT TGCCTGAGCTCTCTAACTGGAACGAGAGATAGAGATGGGACCTGAGCGGATA |
| mm4_intron_Grb2_chr11_115708052 | CAATCACCACGTTAGCTGCGGGCACCGAATCCCCCGCCTCCCGGGCTCAGTCAGCCTGACTCAGAGCAGCAGCCTG |
| mm4_intergenic_BC034090|Xpr1_chr1_155258396 |
TGAGAATGGTGGCAAATCAAAGGATAGTCTCATACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNTCTCTCATGAGAGTGAGGTACCAAACTCTGG |
| mm4_intron_Slit3_chr11_35121723 |
CAGAGCGCCTGTGAGTACCCACCCCGCCCGCCCGCTCAGGCCACCCACCCGGCTGGCTCCTACAGAGTCGCCCTCAGGGC GCAGGTCCTTCTTTCCTCTCCGGTGCATGGGGGACGATCCCTACTCCCCTACCCC |
| mm4_intron_Rab6b_chr9_103113614 |
CCACCTCCTGTCTGCTGATGCTCAGTACCTGAGCTGGAGGGTTGGGAGCCGGGTGGAAGTCGGGGTTGCCTGTCCCGGAG AGCTGGGTTTGGGACATTTCACATCGTACCCAGCTCAGCTAGGTAGCCAGCTGGATCTGA |
| mm4_intron_Nfil3_chr13_52978466 |
GGAGTAGAGTCAGCTTCTGGCTCGGTCTGCCTGAGGCTGCACTGTCCCGAGGCCCAAGGCCACCGGAGCCGGCGCCCAGG AGGCACGCCTGCTCTCCGCCTCAGGCCACCGGCTCCGGGCGAGCGCAGCTCACCGAACTC |