CRISPOR

DNA break repair outcome predictions

Guide: GGAGCGGCCATGCTTGGCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAA`	(Wild-type sequence)
423	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC-------CCAGTCCCCTCCGCGGACAGCCT`	`7 bp deleted → frameshift`
402	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC--------CAGTCCCCTCCGCGGACAGCCT`	`8 bp deleted → frameshift`
382	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC---------AGTCCCCTCCGCGGACAGCCT`	`9 bp deleted → no frameshift`
311	`ACGGTCTGCTGGAGCGGCCATGCTTGG-----GACCCCCAGTCCCCTCCGCGGACAGCCT`	`5 bp deleted → frameshift`
296	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC------CCCAGTCCCCTCCGCGGACAGCCT`	`6 bp deleted → no frameshift`
296	`ACGGTCTGCTGGAGCGGCCATGCTTGG------ACCCCCAGTCCCCTCCGCGGACAGCCT`	`6 bp deleted → no frameshift`
283	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC---------------CTCCGCGGACAGCCT`	`15 bp deleted → no frameshift`
269	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC----------------TCCGCGGACAGCCT`	`16 bp deleted → frameshift`
242	`ACGGTCTGCTGGAGCGGCCATGCTTGGCC----------AGTCCCCTCCGCGGACAGCCT`	`10 bp deleted → frameshift`
198	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC--------------CCTCCGCGGACAGCCT`	`14 bp deleted → frameshift`
188	`ACGGTCTGCTGGAGCGG---------------GACCCCCAGTCCCCTCCGCGGACAGCCT`	`15 bp deleted → no frameshift`
184	`ACGGTCTGCTGGAGCGGCCA--------------------GTCCCCTCCGCGGACAGCCT`	`20 bp deleted → frameshift`
179	`ACGGTCTGCTGGAGCGG----------------ACCCCCAGTCCCCTCCGCGGACAGCCT`	`16 bp deleted → frameshift`
175	`ACGGTCTGCTGGA---------------------CCCCCAGTCCCCTCCGCGGACAGCCT`	`21 bp deleted → no frameshift`
170	`ACGGTCTGCTGGAGCGGCCATGCTTGGCC-----------------TCCGCGGACAGCCT`	`17 bp deleted → frameshift`
170	`ACGGTCTGCTGGAGCGGCC-----------------CCCAGTCCCCTCCGCGGACAGCCT`	`17 bp deleted → frameshift`
162	`ACGGTCTGCTGGAGCGGCC------------------CCAGTCCCCTCCGCGGACAGCCT`	`18 bp deleted → no frameshift`
154	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC-------------------GCGGACAGCCT`	`19 bp deleted → frameshift`
154	`ACGGTCTGCTGGAGCGGCC-------------------CAGTCCCCTCCGCGGACAGCCT`	`19 bp deleted → frameshift`
147	`ACGGTCTGCTGGAGCGGCCATGCTTGGCC--------------------GCGGACAGCCT`	`20 bp deleted → frameshift`
147	`ACGGTCTGCTGG--------------------GACCCCCAGTCCCCTCCGCGGACAGCCT`	`20 bp deleted → frameshift`
133	`ACGGTCTGCTGGAGCGGCCATGCTTGGCC------------------------------T`	`30 bp deleted → no frameshift`
132	`ACGGTCTGCTGGAGCGG------------------------------------ACAGCCT`	`36 bp deleted → no frameshift`
126	`ACGGTCTGCTGGAGCGGCCATGCTTGGC-----------------------GGACAGCCT`	`23 bp deleted → frameshift`
114	`ACGGTCTGCTGGAGCGGCC-------------------------CCTCCGCGGACAGCCT`	`25 bp deleted → frameshift`
109	`ACGGTCTGCTGGAGCGGCCATGCTTGG--------------------------ACAGCCT`	`26 bp deleted → frameshift`
109	`ACGGTCTGCTGGAGCGGCC--------------------------CTCCGCGGACAGCCT`	`26 bp deleted → frameshift`
103	`ACGGTCTGCTGGAGCGGCC---------------------------TCCGCGGACAGCCT`	`27 bp deleted → no frameshift`
98	`ACGGTCTGCTGGAGCGGCCATGC----------------------------GGACAGCCT`	`28 bp deleted → frameshift`
98	`ACGG----------------------------GACCCCCAGTCCCCTCCGCGGACAGCCT`	`28 bp deleted → frameshift`
95	`ACGGTCTGCTGGAGCGGCCATGCT-----------------------CCGCGGACAGCCT`	`23 bp deleted → frameshift`
94	`ACGGTCTGCTGGAGCGGCCATGCTTGGCCC-----------------------------T`	`29 bp deleted → frameshift`
94	`ACGG-----------------------------ACCCCCAGTCCCCTCCGCGGACAGCCT`	`29 bp deleted → frameshift`
89	`ACGGTCTGCTGGAGCGGCC------------------------------GCGGACAGCCT`	`30 bp deleted → no frameshift`
81	`ACGGTCTGCTGGAGCGGCC----------------------------------------T`	`40 bp deleted → frameshift`
78	`ACGGTC-------------------------------------CCCTCCGCGGACAGCCT`	`37 bp deleted → frameshift`
77	`ACGGTCTGCTGGAG---------------------------TCCCCTCCGCGGACAGCCT`	`27 bp deleted → no frameshift`
76	`ACGGTCTGCTGGAGCGGC---------------------------------GGACAGCCT`	`33 bp deleted → no frameshift`
73	`ACGGTCTGCTGGAGCG----------------------------------CGGACAGCCT`	`34 bp deleted → frameshift`
69	`ACGGTCTGCTGGAGCGGCCATGC-----------------------------------CT`	`35 bp deleted → frameshift`
64	`ACGGTCTGCTGGA-----------------------------------------CAGCCT`	`41 bp deleted → frameshift`
58	`ACGGTCTGCTGGAGC-------------------------------------------CT`	`43 bp deleted → frameshift`
57	`AC---------------------------------CCCCAGTCCCCTCCGCGGACAGCCT`	`33 bp deleted → no frameshift`
51	`ACGG-------------------------------------------------ACAGCCT`	`49 bp deleted → frameshift`
49	`ACGGTCTGCTGGAGCGGCCATGCT------------------------------------`	`36 bp deleted → no frameshift`
49	`ACGGTCTGCTGGAGCGGCCA------------------------------------GCCT`	`36 bp deleted → no frameshift`
48	`ACGGTCTGC------------------------------------------GGACAGCCT`	`42 bp deleted → no frameshift`
47	`ACGGTCTGCT-------------------------------------CCGCGGACAGCCT`	`37 bp deleted → frameshift`
40	`ACGGTCT----------------------------------------CCGCGGACAGCCT`	`40 bp deleted → frameshift`
38	`ACG-----------------------------------------------CGGACAGCCT`	`47 bp deleted → frameshift`
36	`ACGGTC------------------------------------------CGCGGACAGCCT`	`42 bp deleted → no frameshift`
34	`ACGGTCTGC-------------------------------------------------CT`	`49 bp deleted → frameshift`
24	`ACGGTCTGCT--------------------------------------------------`	`50 bp deleted → frameshift`
21	`ACGGTCT-----------------------------------------------------`	`53 bp deleted → frameshift`
21	`AC-----------------------------------------------------AGCCT`	`53 bp deleted → frameshift`

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