CRISPOR

DNA break repair outcome predictions

Guide: TCCCAGGCTCCCTGGGATCT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGG`	(Wild-type sequence)
335	`AAGCTCCTAATCCCAGGCTCCCTGG--------CTGAGAAGAGGAGCTAAGGCGACAAGG`	`8 bp deleted → frameshift`
314	`AAGCTCCTAATCCCAGGCT----------------GAGAAGAGGAGCTAAGGCGACAAGG`	`16 bp deleted → frameshift`
282	`AAGCTCCTAATCCCAGGCTCCCTGGG-------CTGAGAAGAGGAGCTAAGGCGACAAGG`	`7 bp deleted → frameshift`
274	`AAGCTCCTAATCCCAGGCTCCCTG------------AGAAGAGGAGCTAAGGCGACAAGG`	`12 bp deleted → no frameshift`
233	`AAGCTCCTAATCCCAGGCTCCCTGGGATCT-----GAGAAGAGGAGCTAAGGCGACAAGG`	`5 bp deleted → frameshift`
203	`AAGCTCCTAATCCCAGGCTCCCTGGGA------------------GCTAAGGCGACAAGG`	`18 bp deleted → no frameshift`
182	`AAGCTCCTAATCCCAGGCTCCCTGGGA----------GAAGAGGAGCTAAGGCGACAAGG`	`10 bp deleted → frameshift`
164	`AAGCTCCTAATCCCAGGCTCCCTGGGA------------AGAGGAGCTAAGGCGACAAGG`	`12 bp deleted → no frameshift`
154	`AAGCTCCTAATCCCAGGCTCCCTGG-------------------AGCTAAGGCGACAAGG`	`19 bp deleted → frameshift`
141	`AAGCTCCTAATCCCAGGCTCCCTGGGA---------------GGAGCTAAGGCGACAAGG`	`15 bp deleted → no frameshift`
129	`AAGCTCCTAATCCCAGG---------------------------AGCTAAGGCGACAAGG`	`27 bp deleted → no frameshift`
122	`AAGCTCCTAATCCCAGGCTCCCTGGGATCT------------------AAGGCGACAAGG`	`18 bp deleted → no frameshift`
121	`AAGCTCCTAATCCCAGGC-----------------------------------GACAAGG`	`35 bp deleted → frameshift`
117	`AAGCTCCTAATCCCAGGCT-----------------------------AAGGCGACAAGG`	`29 bp deleted → frameshift`
111	`AAGCT------------------------------GAGAAGAGGAGCTAAGGCGACAAGG`	`30 bp deleted → no frameshift`
109	`AAGCTCCTAATCCCAGGCTCCCTGGG--------------------------CGACAAGG`	`26 bp deleted → frameshift`
103	`AAGCTCCTAATCCCAGGCTCCCTGG---------------------------CGACAAGG`	`27 bp deleted → no frameshift`
99	`AAGCTCCTAATCCCAG----------------------AAGAGGAGCTAAGGCGACAAGG`	`22 bp deleted → frameshift`
86	`AAGCTCCTAATCCCAGGCTCCCT-------------------------AAGGCGACAAGG`	`25 bp deleted → frameshift`
86	`AAGCTCCTAATCCCAG-------------------------AGGAGCTAAGGCGACAAGG`	`25 bp deleted → frameshift`
77	`AAGCTCCT---------------------------GAGAAGAGGAGCTAAGGCGACAAGG`	`27 bp deleted → no frameshift`
74	`AAGCTCCTAATCCCAGGCTCCCTGGGA----------------------------CAAGG`	`28 bp deleted → frameshift`
73	`AAGCTCCTAATCCCAGGCTCCCTGGG----------------------------------`	`34 bp deleted → frameshift`
69	`AAGCT-------------------------------------------AAGGCGACAAGG`	`43 bp deleted → frameshift`
69	`AAGCTCCTAATCCCAGGCTCCCTGG-----------------------------------`	`35 bp deleted → frameshift`
67	`AAGCTCCTAA----------------------------------------GGCGACAAGG`	`40 bp deleted → frameshift`
66	`AAGCTCCTAATCCCAG------------------------------CTAAGGCGACAAGG`	`30 bp deleted → no frameshift`
60	`AAG--------------------------------------AGGAGCTAAGGCGACAAGG`	`38 bp deleted → frameshift`
58	`AAGCTCCTAATCCCAGG-------------------------------------------`	`43 bp deleted → frameshift`
52	`AAG-----------------------------------AAGAGGAGCTAAGGCGACAAGG`	`35 bp deleted → frameshift`
44	`AAGCTCCTAA------------------------------GAGGAGCTAAGGCGACAAGG`	`30 bp deleted → no frameshift`
40	`AAG----------------------------------------GAGCTAAGGCGACAAGG`	`40 bp deleted → frameshift`
36	`AAGCTCCTAATCCCA------------------------------------------AGG`	`42 bp deleted → no frameshift`
36	`AAG------------------------------------------------GCGACAAGG`	`48 bp deleted → no frameshift`
34	`AAGC-------------------------------------------------GACAAGG`	`49 bp deleted → frameshift`
24	`AAG--------------------------------------------------------G`	`56 bp deleted → frameshift`
18	`AAGCTCCTAA------------------------------------------------GG`	`48 bp deleted → no frameshift`

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