← return to the list of all guides
The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| CTGCTGCAATCGTCATTACAGTACCGCTGGTGACGCCACTCGGCGAACGCAAGTGGATAAATAGAAACGTCTGCCACAGT | (Wild-type sequence) | |
| 347 | CGTCATTACAGTACCGCTGGTGACGC--------------AAGTGGATAAATAGAAACGT | 14 bp deleted → frameshift |
| 282 | CGTCATTACAGTACCGCTGGTGACG-------GCGAACGCAAGTGGATAAATAGAAACGT | 7 bp deleted → frameshift |
| 268 | CGTCATTACAGTACCGCTGGTGACGC--------GAACGCAAGTGGATAAATAGAAACGT | 8 bp deleted → frameshift |
| 242 | CGTCATTACAGTACCGCTGGTGACG----------AACGCAAGTGGATAAATAGAAACGT | 10 bp deleted → frameshift |
| 208 | CGTCATTACAGTACCGCTGG-------------CGAACGCAAGTGGATAAATAGAAACGT | 13 bp deleted → frameshift |
| 191 | CGTCATTACAGTACCGCTGGTGACGCCAC---------GCAAGTGGATAAATAGAAACGT | 9 bp deleted → no frameshift |
| 190 | CGTCATTACAGTACCGC-----------------------AAGTGGATAAATAGAAACGT | 23 bp deleted → frameshift |
| 179 | CGTCATTACAGTACCG----------------GCGAACGCAAGTGGATAAATAGAAACGT | 16 bp deleted → frameshift |
| 170 | CGTCATTACAGTACCGC-----------------GAACGCAAGTGGATAAATAGAAACGT | 17 bp deleted → frameshift |
| 158 | CGTCATTACAGTACCGCTGGTG-----------------------GATAAATAGAAACGT | 23 bp deleted → frameshift |
| 156 | CGTCATTACAGTACCGCTGGTGACGCCA-------------AGTGGATAAATAGAAACGT | 13 bp deleted → frameshift |
| 156 | CGTCATTACAGTACCGCTGGTGA-------------ACGCAAGTGGATAAATAGAAACGT | 13 bp deleted → frameshift |
| 154 | CGTCATTACAGTACCG-------------------AACGCAAGTGGATAAATAGAAACGT | 19 bp deleted → frameshift |
| 136 | CGTCATTACAGTACCGCTGG--------------------------ATAAATAGAAACGT | 26 bp deleted → frameshift |
| 91 | CGTCATTACAGTACCGCTGGTGACG----------------------------------T | 34 bp deleted → frameshift |
| 90 | CGTCATTACAGTACCGCTGGTGA------------------------TAAATAGAAACGT | 24 bp deleted → no frameshift |
| 90 | CGTCATTACAGTAC------------------------GCAAGTGGATAAATAGAAACGT | 24 bp deleted → no frameshift |
| 89 | CG------------------------------GCGAACGCAAGTGGATAAATAGAAACGT | 30 bp deleted → no frameshift |
| 80 | CGTCATTACAGT--------------------------------GGATAAATAGAAACGT | 32 bp deleted → frameshift |
| 76 | CG---------------------------------AACGCAAGTGGATAAATAGAAACGT | 33 bp deleted → no frameshift |
| 70 | CGTCATTACAGTACCGCTGGTGACGCCAC-----------------------------GT | 29 bp deleted → frameshift |
| 70 | CGTCATTAC-----------------------------GCAAGTGGATAAATAGAAACGT | 29 bp deleted → frameshift |
| 63 | CGTCATTACA-------------------------------AGTGGATAAATAGAAACGT | 31 bp deleted → frameshift |
| 62 | CG-------------------------------------CAAGTGGATAAATAGAAACGT | 37 bp deleted → frameshift |
| 60 | CGTCATTACAGTACCGCTGGTGA--------------------------------AACGT | 32 bp deleted → frameshift |
| 49 | CGTCA------------------------------------AGTGGATAAATAGAAACGT | 36 bp deleted → no frameshift |
| 46 | CGTCATTACAGTACCG-------------------------------------------T | 43 bp deleted → frameshift |
| 42 | CGTCATTACAGTACCGCTGGT--------------------------------------- | 39 bp deleted → no frameshift |
| 38 | CGT-----------------------------------------GGATAAATAGAAACGT | 41 bp deleted → frameshift |
| 34 | CGTCATTACAG-------------------------------------------AAACGT | 43 bp deleted → frameshift |
| 33 | CGTCATTACAGTAC--------------------------------------------GT | 44 bp deleted → frameshift |
| 33 | CGTCATTACAGTA------------------------------------AATAGAAACGT | 36 bp deleted → no frameshift |
| 29 | CGT--------------------------------------------------------- | 57 bp deleted → no frameshift |
| 27 | CGTCATTACAGT------------------------------------------------ | 48 bp deleted → no frameshift |
| 27 | CGTCATTACAGTA----------------------------------------GAAACGT | 40 bp deleted → frameshift |
| 25 | CGTCATTA-----------------------------------------AATAGAAACGT | 41 bp deleted → frameshift |
| 25 | CGTCATTAC-------------------------------------------------GT | 49 bp deleted → frameshift |
| 24 | CGTCAT------------------------------------------AAATAGAAACGT | 42 bp deleted → no frameshift |
| 21 | CGTCATTA---------------------------------------------GAAACGT | 45 bp deleted → no frameshift |
| 20 | CGTCAT----------------------------------------------AGAAACGT | 46 bp deleted → frameshift |