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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| TCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCC | (Wild-type sequence) | |
| 741 | TCTCCATCCCCGGACTCTCCAGAGGGGGGG------AAAACCCCCAAGTAAGCTCCTAAT | 6 bp deleted → no frameshift |
| 737 | TCTCCATCCCCGGACTCTCCAGAGGGGG--------AAAACCCCCAAGTAAGCTCCTAAT | 8 bp deleted → frameshift |
| 705 | TCTCCATCCCCGGACTCTCCAGAGGGGGG-------AAAACCCCCAAGTAAGCTCCTAAT | 7 bp deleted → frameshift |
| 623 | TCTCCATCCCCGGACTCTCCAGAGGGGGGG-----GAAAACCCCCAAGTAAGCTCCTAAT | 5 bp deleted → frameshift |
| 510 | TCTCCATCCCCGGACTCTCCAGAGGGG---------AAAACCCCCAAGTAAGCTCCTAAT | 9 bp deleted → no frameshift |
| 491 | TCTCCATCCCCGGACTCTCCAGAGGGGGGG----GGAAAACCCCCAAGTAAGCTCCTAAT | 4 bp deleted → frameshift |
| 364 | TCTCCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCCTAAT | 10 bp deleted → frameshift |
| 344 | TCTCCATCCCCGGACTCTCCAGAGGGGGGG---GGGAAAACCCCCAAGTAAGCTCCTAAT | 3 bp deleted → no frameshift |
| 230 | TCTCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCCTAAT | 11 bp deleted → frameshift |
| 182 | TCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGCTCCTAAT | 10 bp deleted → frameshift |
| 158 | TCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCCTAAT | 23 bp deleted → frameshift |
| 153 | TCTCCATCCCC---------------------------------CAAGTAAGCTCCTAAT | 33 bp deleted → no frameshift |
| 149 | TCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCCTAAT | 14 bp deleted → frameshift |
| 147 | TCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCTCCTAAT | 20 bp deleted → frameshift |
| 146 | TCTCCATCCCC----------------------------------AAGTAAGCTCCTAAT | 34 bp deleted → frameshift |
| 143 | TCTCCATCCCCGGACTCTCCA-------------------------AGTAAGCTCCTAAT | 25 bp deleted → frameshift |
| 140 | TCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTCCTAAT | 21 bp deleted → no frameshift |
| 133 | TCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCCTAAT | 22 bp deleted → frameshift |
| 133 | TCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCCTAAT | 22 bp deleted → frameshift |
| 126 | TCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCCTAAT | 23 bp deleted → frameshift |
| 121 | TCTCCATCCCC--------------------------------CCAAGTAAGCTCCTAAT | 32 bp deleted → frameshift |
| 120 | TCTCCATCCCCGGACTCTCC------------------------CAAGTAAGCTCCTAAT | 24 bp deleted → no frameshift |
| 115 | TCTCCATCCCCGGACTCTCC------------------------------------TAAT | 36 bp deleted → no frameshift |
| 104 | TCTCCATCCC-----------------------------------AAGTAAGCTCCTAAT | 35 bp deleted → frameshift |
| 90 | TCTCCATCCCCGGACTCTCCAGAG------------------------TAAGCTCCTAAT | 24 bp deleted → no frameshift |
| 84 | TCTCCATCCCC-------------------------------CCCAAGTAAGCTCCTAAT | 31 bp deleted → frameshift |
| 81 | TCTCCATCCCCGGACTCTCCAG--------------------------TAAGCTCCTAAT | 26 bp deleted → frameshift |
| 81 | TCTCCATCCCCGGAC--------------------------CCCCAAGTAAGCTCCTAAT | 26 bp deleted → frameshift |
| 75 | TCTCCATCCCCGGACTC--------------------------------------CTAAT | 38 bp deleted → frameshift |
| 74 | TCTCCATCCCCGGACTCTCCAGAG----------------------------CTCCTAAT | 28 bp deleted → frameshift |
| 67 | TCTCCA----------------------------------------AGTAAGCTCCTAAT | 40 bp deleted → frameshift |
| 66 | TCTCCATCCCCGGACTCTCCAG------------------------------CTCCTAAT | 30 bp deleted → no frameshift |
| 66 | TCTCCATCC------------------------------------AAGTAAGCTCCTAAT | 36 bp deleted → no frameshift |
| 62 | TCTCC-------------------------------------CCCAAGTAAGCTCCTAAT | 37 bp deleted → frameshift |
| 60 | TCTCC--------------------------------------CCAAGTAAGCTCCTAAT | 38 bp deleted → frameshift |
| 56 | TCTCC---------------------------------------CAAGTAAGCTCCTAAT | 39 bp deleted → no frameshift |
| 54 | TCTCC---------------------------------------------------TAAT | 51 bp deleted → no frameshift |
| 47 | TCTCCATCC-----------------------------------------------TAAT | 47 bp deleted → frameshift |
| 42 | TCTCCATCCCCGGACTCT---------------------------------------AAT | 39 bp deleted → no frameshift |
| 42 | TCTCCATCCCC---------------------------------------------TAAT | 45 bp deleted → no frameshift |
| 40 | TCTCCATCCC----------------------------------------------TAAT | 46 bp deleted → frameshift |
| 38 | TCTCCATCCCCGGACT-----------------------------------------AAT | 41 bp deleted → frameshift |
| 21 | TC-----------------------------------------------------CTAAT | 53 bp deleted → frameshift |
| 20 | TCT------------------------------------------------------AAT | 54 bp deleted → no frameshift |
| 14 | TCTCCAT----------------------------------------------------- | 53 bp deleted → frameshift |