CRISPOR

DNA break repair outcome predictions

Guide: TCGCGGGCTCAGGCAACCGT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCT`	(Wild-type sequence)
403	`GAGGGCCGGCTCGCGGGCTCAGGCA-----------CCATCTTCTCTGGCGGTTCAGGCA`	`11 bp deleted → frameshift`
341	`GAGGGCCGGCTCGCGGGC-----------------ACCATCTTCTCTGGCGGTTCAGGCA`	`17 bp deleted → frameshift`
303	`GAGGGCCGGCTCGCGGGCTCAGGCAACC----------ATCTTCTCTGGCGGTTCAGGCA`	`10 bp deleted → frameshift`
269	`GAGGGCCGGCTCGCGGG----------------GCACCATCTTCTCTGGCGGTTCAGGCA`	`16 bp deleted → frameshift`
255	`GAGGGCCGGCTCGCGGGCTCAGG---------GGCACCATCTTCTCTGGCGGTTCAGGCA`	`9 bp deleted → no frameshift`
242	`GAGGGCCGGCTCGCGGGCTCAGG----------GCACCATCTTCTCTGGCGGTTCAGGCA`	`10 bp deleted → frameshift`
191	`GAGGGCCGGCTCGCGGGCTCAGGCA-----------------------------------`	`35 bp deleted → frameshift`
188	`GAGGGCCGGCTCGCGGG---------------GGCACCATCTTCTCTGGCGGTTCAGGCA`	`15 bp deleted → no frameshift`
188	`GAGGGC-----------------------------ACCATCTTCTCTGGCGGTTCAGGCA`	`29 bp deleted → frameshift`
172	`GAGGGCCGGC-------------------------ACCATCTTCTCTGGCGGTTCAGGCA`	`25 bp deleted → frameshift`
163	`GAGGGCCGGCTCGCGGGCTCAGGC--------------------------GGTTCAGGCA`	`26 bp deleted → frameshift`
162	`GAGGGCCGGCTCGCGG------------------CACCATCTTCTCTGGCGGTTCAGGCA`	`18 bp deleted → no frameshift`
149	`GAGGGCCGGCTCGCGGGCTCAGGCA--------------TCTTCTCTGGCGGTTCAGGCA`	`14 bp deleted → frameshift`
148	`GAGGG----------------------------GCACCATCTTCTCTGGCGGTTCAGGCA`	`28 bp deleted → frameshift`
141	`GAGGGCCGGCTCGCGGGCTCA---------------CCATCTTCTCTGGCGGTTCAGGCA`	`15 bp deleted → no frameshift`
140	`GAGGGCCGGCTCGC---------------------ACCATCTTCTCTGGCGGTTCAGGCA`	`21 bp deleted → no frameshift`
136	`GAGGGCCGGCTCGCGGGCTC--------------------------TGGCGGTTCAGGCA`	`26 bp deleted → frameshift`
133	`GAGGGCCGGCTCGCGGGCTCAGGCAACCG----------------------GTTCAGGCA`	`22 bp deleted → frameshift`
132	`GAGGGCCGGCTCGCGG------------------------------------TTCAGGCA`	`36 bp deleted → no frameshift`
126	`GAGGGCCGG-----------------------GGCACCATCTTCTCTGGCGGTTCAGGCA`	`23 bp deleted → frameshift`
122	`GAGGGCCGGCTCGCGGGCTCA------------------TCTTCTCTGGCGGTTCAGGCA`	`18 bp deleted → no frameshift`
121	`GAGGGCCGGCTCGCGGGC--------------------------------GGTTCAGGCA`	`32 bp deleted → frameshift`
120	`GAGGGCCGG------------------------GCACCATCTTCTCTGGCGGTTCAGGCA`	`24 bp deleted → no frameshift`
105	`GAGGGCCGGCTCGCGGGCTC---------------------TTCTCTGGCGGTTCAGGCA`	`21 bp deleted → no frameshift`
103	`GAGGG---------------------------GGCACCATCTTCTCTGGCGGTTCAGGCA`	`27 bp deleted → no frameshift`
95	`GAGGGCCGGCTCGCGGGCTCAGGCAACCGT-----------------------TCAGGCA`	`23 bp deleted → frameshift`
95	`GAGGGCCGGCTCGCGGGCT-----------------------TCTCTGGCGGTTCAGGCA`	`23 bp deleted → frameshift`
94	`GAGGGCCGGCTCGCGGGCTCAGG-----------------------------TTCAGGCA`	`29 bp deleted → frameshift`
91	`GAGGGCCGGCTC----------------------------------TGGCGGTTCAGGCA`	`34 bp deleted → frameshift`
90	`GAGGGCCGGCTCGCGGGCTC------------------------TCTGGCGGTTCAGGCA`	`24 bp deleted → no frameshift`
89	`GAGG------------------------------CACCATCTTCTCTGGCGGTTCAGGCA`	`30 bp deleted → no frameshift`
84	`GAGGGCC-------------------------------ATCTTCTCTGGCGGTTCAGGCA`	`31 bp deleted → frameshift`
81	`GAGGGCCGGC----------------------------------------GGTTCAGGCA`	`40 bp deleted → frameshift`
77	`GAGGGCCGGCTCGCGGGC-----------------------------------------A`	`41 bp deleted → frameshift`
76	`GAGGGCCGGCTCGCGG---------------------------------CGGTTCAGGCA`	`33 bp deleted → no frameshift`
74	`GAGGGCCGGCTCGCGGGCT----------------------------GGCGGTTCAGGCA`	`28 bp deleted → frameshift`
70	`GAGGGCCGGCTC-----------------------------TTCTCTGGCGGTTCAGGCA`	`29 bp deleted → frameshift`
69	`GAGGGCCGG-------------------------------------------TTCAGGCA`	`43 bp deleted → frameshift`
69	`GAGGGCCGGCTCGCGGG-----------------------------------TTCAGGCA`	`35 bp deleted → frameshift`
66	`GAGGGC--------------------------------------------GGTTCAGGCA`	`44 bp deleted → frameshift`
63	`GAGGGCCGGCTCGCGGGCTCAGGCA-------------------------------GGCA`	`31 bp deleted → frameshift`
63	`GAGGGCCGGCT-------------------------------TCTCTGGCGGTTCAGGCA`	`31 bp deleted → frameshift`
60	`GAGGGCCGGCTC--------------------------------TCTGGCGGTTCAGGCA`	`32 bp deleted → frameshift`
60	`GAGGGCCGGCTCG--------------------------------------GTTCAGGCA`	`38 bp deleted → frameshift`
51	`GAGGGCCGGC-------------------------------------------------A`	`49 bp deleted → frameshift`
49	`GAGGGCCGGCT------------------------------------GGCGGTTCAGGCA`	`36 bp deleted → no frameshift`
48	`GAGGGCCGGCTCGCGG------------------------------------------CA`	`42 bp deleted → no frameshift`
42	`GAGGGCCGGCTCGCGGGCTCA---------------------------------------`	`39 bp deleted → no frameshift`
42	`GAGGGC-----------------------------------------------------A`	`53 bp deleted → frameshift`
42	`GAGGGCCGGCTCGC---------------------------------------------A`	`45 bp deleted → no frameshift`
42	`GAGG---------------------------------------------CGGTTCAGGCA`	`45 bp deleted → no frameshift`
38	`GAGGG-----------------------------------------------TTCAGGCA`	`47 bp deleted → frameshift`
36	`GAGG------------------------------------------------TTCAGGCA`	`48 bp deleted → no frameshift`
34	`GAGGGCCGGCTC-------------------------------------------AGGCA`	`43 bp deleted → frameshift`
33	`GAGG------------------------------------------------------CA`	`54 bp deleted → no frameshift`

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