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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313572_F | TCGTCGGCAGCGTCTCACTGTGGCAGACGTTTCT | 59.5 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313572_R | GTCTCGTGGGCTCGGGCCCCATTGCCCTCTTCTTT | 60.6 | 1.00 |
| mm3_intergenic_Gm22126|Cdyl_chr13_35669786_F | TCGTCGGCAGCGTCCAGAATGCTGGCTGGCTTTC | 59.8 | 0.55 |
| mm3_intergenic_Gm22126|Cdyl_chr13_35669786_R | GTCTCGTGGGCTCGGGCCTCTGTTCTCTGGTCACC | 60.0 | 0.55 |
| mm4_intergenic_Gm12636|Gm12632_chr4_90752652_F | TCGTCGGCAGCGTCTAACCTAGGTCCAGGCCTGT | 59.5 | 0.48 |
| mm4_intergenic_Gm12636|Gm12632_chr4_90752652_R | GTCTCGTGGGCTCGGGGGTAGTGCACACAAGGTCA | 59.8 | 0.48 |
| mm4_intergenic_Gm13575|Gm13577_chr2_64408270_F | TCGTCGGCAGCGTCGGTGTAAGGAAAGCTGCCAC | 59.1 | 0.42 |
| mm4_intergenic_Gm13575|Gm13577_chr2_64408270_R | GTCTCGTGGGCTCGGGCACAAAAGCAGATGGTGGT | 59.3 | 0.42 |
| mm4_intergenic_Gm25258|Hao1_chr2_134076800_F | TCGTCGGCAGCGTCGTAACCTCCGACCTTTGCCA | 59.9 | 0.36 |
| mm4_intergenic_Gm25258|Hao1_chr2_134076800_R | GTCTCGTGGGCTCGGCCACGGAAGACTACCCACAG | 59.7 | 0.36 |
| mm3_intron_Cntn4_chr6_106197020_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm3_intron_Cntn4_chr6_106197020_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Fars2|Gm26395_chr13_36611183_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Fars2|Gm26395_chr13_36611183_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_4930563I02Rik_chr14_60089959_F | TCGTCGGCAGCGTCTGAGAAGGGCCAGCTTTGAG | 59.9 | 0.27 |
| mm4_intron_4930563I02Rik_chr14_60089959_R | GTCTCGTGGGCTCGGTGTGTCTAGACTGCACAGCTG | 60.0 | 0.27 |
| mm4_exon_Vopp1_chr6_57729713_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Vopp1_chr6_57729713_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm23325|Pak3_chrX_143460694_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm23325|Pak3_chrX_143460694_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Rps6ka1|Hmgn2_chr4_133898443_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Rps6ka1|Hmgn2_chr4_133898443_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Rcan2_chr17_43908587_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Rcan2_chr17_43908587_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Cept1_chr3_106546921_F | TCGTCGGCAGCGTCTGAACACATTCCTGACAGTTGT | 58.1 | 0.20 |
| mm4_intron_Cept1_chr3_106546921_R | GTCTCGTGGGCTCGGGCATTGCTAAAACTCTAACTCACCA | 59.8 | 0.20 |
| mm4_intergenic_Gm16129|Plch1_chr3_63847742_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm16129|Plch1_chr3_63847742_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Lmcd1|5031434C07Rik_chr6_112281065_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Lmcd1|5031434C07Rik_chr6_112281065_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22891|Gm24774_chr14_82429165_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22891|Gm24774_chr14_82429165_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Lrtm2_chr6_119329785_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Lrtm2_chr6_119329785_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Psd3_chr8_67860993_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Psd3_chr8_67860993_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Ccdc132_chr6_3538122_F | TCGTCGGCAGCGTCAAATGGACAGCCTGTGTGGT | 59.8 | 0.16 |
| mm4_intron_Ccdc132_chr6_3538122_R | GTCTCGTGGGCTCGGAGGTGGCACTATGAAGGCAC | 60.0 | 0.16 |
| mm4_intron_Prickle1_chr15_93512315_F | TCGTCGGCAGCGTCCCTATCAGAAACGCCTGTGGT | 60.0 | 0.15 |
| mm4_intron_Prickle1_chr15_93512315_R | GTCTCGTGGGCTCGGCCAGGGAAGTGTGGAACCTC | 59.9 | 0.15 |
| mm4_intron_Cyp2c66_chr19_39128558_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Cyp2c66_chr19_39128558_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Olfr1416|Olfr1415_chr1_92487629_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Olfr1416|Olfr1415_chr1_92487629_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm13583/Pla2r1|Gm13566_chr2_60564668_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm13583/Pla2r1|Gm13566_chr2_60564668_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Sstr5|Sox8_chr17_25537569_F | TCGTCGGCAGCGTCGCTCCCTGCTTCTGATGGTA | 59.1 | 0.14 |
| mm4_intergenic_Sstr5|Sox8_chr17_25537569_R | GTCTCGTGGGCTCGGAGTGGTCAGGACTCTTCCCA | 59.8 | 0.14 |
| mm4_exon_Gm13607_chr2_68082227_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Gm13607_chr2_68082227_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rad51b|Zfp36l1/Gm26669_chr12_79660616_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Rad51b|Zfp36l1/Gm26669_chr12_79660616_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_9130015L21Rik|Gm826_chr2_160233630_F | TCGTCGGCAGCGTCAAGAGCCTTTGCCTCATCCC | 60.0 | 0.11 |
| mm4_intergenic_9130015L21Rik|Gm826_chr2_160233630_R | GTCTCGTGGGCTCGGGGATCCTGAGGTGGTGTTGG | 60.0 | 0.11 |
| mm4_intergenic_Olfr60|Olfr530_chr7_140349735_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Olfr60|Olfr530_chr7_140349735_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Fabp3_chr4_130313452_F | TCGTCGGCAGCGTCTCCACTGGGATGTCTGGACA | 60.1 | 0.10 |
| mm4_intron_Fabp3_chr4_130313452_R | GTCTCGTGGGCTCGGAAGCAGGGTTTGTCGGTAGG | 59.9 | 0.10 |
| mm3_intergenic_Gm5471|Kcnv1_chr15_45057626_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Gm5471|Kcnv1_chr15_45057626_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Olfr420|Spta1_chr1_174170615_F | TCGTCGGCAGCGTCCCTGAAAGGGACCATGTCACA | 59.9 | 0.09 |
| mm4_intergenic_Olfr420|Spta1_chr1_174170615_R | GTCTCGTGGGCTCGGCTGCCCTCATCCACCAATGA | 59.7 | 0.09 |
| mm4_intergenic_Gm12075|Gm12077_chr11_27486412_F | TCGTCGGCAGCGTCAGCAGCTAGTGACAATCTGCA | 59.7 | 0.07 |
| mm4_intergenic_Gm12075|Gm12077_chr11_27486412_R | GTCTCGTGGGCTCGGATGGATGCTGCTGCTTCACT | 60.0 | 0.07 |
| mm4_intron_Cdk19_chr10_40432116_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Cdk19_chr10_40432116_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Map3k5_chr10_20112146_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Map3k5_chr10_20112146_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109009247_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109009247_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Rimbp2_chr5_128898553_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Rimbp2_chr5_128898553_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm5407|Gm6931_chr16_49400839_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm5407|Gm6931_chr16_49400839_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_M6pr-ps|Gm12448_chr3_99507614_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_M6pr-ps|Gm12448_chr3_99507614_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Macc1|Gm25675_chr12_119775537_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Macc1|Gm25675_chr12_119775537_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Zbtb8b_chr4_129430544_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Zbtb8b_chr4_129430544_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mitf|Gm765_chr6_98107778_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mitf|Gm765_chr6_98107778_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Parp4_chr14_56632543_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Parp4_chr14_56632543_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm10532_chr18_75517030_F | TCGTCGGCAGCGTCGGCCTCTGTTCTTGACTGGG | 60.3 | 0.03 |
| mm4_intron_Gm10532_chr18_75517030_R | GTCTCGTGGGCTCGGATCTGTGTCTGGCTGTCTGC | 60.0 | 0.03 |
| mm4_intergenic_9530026F06Rik|Gm11588_chr1_61407344_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_9530026F06Rik|Gm11588_chr1_61407344_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Nsmce2_chr15_59448193_F | TCGTCGGCAGCGTCACTCCTGTGATCTCCCCTCC | 60.0 | 0.03 |
| mm3_intron_Nsmce2_chr15_59448193_R | GTCTCGTGGGCTCGGCCACTGGGCAAAGTCAAGATC | 59.1 | 0.03 |
| mm4_intron_Galnt18_chr7_111749706_F | TCGTCGGCAGCGTCTGCAAGGAAATGGACTGGCT | 59.8 | 0.02 |
| mm4_intron_Galnt18_chr7_111749706_R | GTCTCGTGGGCTCGGTTGGAACCTTTTGGCCCTGT | 60.0 | 0.02 |
| mm3_intergenic_5430402E10Rik|Gm14750_chrX_77953978_F | TCGTCGGCAGCGTCCAGCTCCTCTGGTTGTGTCC | 60.3 | 0.02 |
| mm3_intergenic_5430402E10Rik|Gm14750_chrX_77953978_R | GTCTCGTGGGCTCGGACACCACTGTGACCATGGTT | 59.4 | 0.02 |
| mm4_intergenic_Ankrd9|Rcor1_chr12_111013006_F | TCGTCGGCAGCGTCACTGTTGTTGGCCCAGAGAA | 59.4 | 0.02 |
| mm4_intergenic_Ankrd9|Rcor1_chr12_111013006_R | GTCTCGTGGGCTCGGCTGCACACCACAGAACATGC | 60.0 | 0.02 |
| mm4_intergenic_Vma21|Gm1141_chrX_71893301_F | TCGTCGGCAGCGTCGGCTCTCCCCATCTTCAAGG | 59.8 | 0.02 |
| mm4_intergenic_Vma21|Gm1141_chrX_71893301_R | GTCTCGTGGGCTCGGACTGATCTATGGGACGTTTCCA | 58.8 | 0.02 |
| mm4_intergenic_Rsl24d1/Gm20509|Rsl24d1_chr9_73121100_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rsl24d1/Gm20509|Rsl24d1_chr9_73121100_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm5123|Dusp21_chrX_18090641_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm5123|Dusp21_chrX_18090641_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15399|Gm9372_chr3_124205234_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15399|Gm9372_chr3_124205234_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm13291|Gm13292_chr2_11387293_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm13291|Gm13292_chr2_11387293_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_exon_Gm13291_chr2_11386724_F | TCGTCGGCAGCGTCACCTGGCTGCAGTGATCATC | 60.1 | 0.02 |
| mm3_exon_Gm13291_chr2_11386724_R | GTCTCGTGGGCTCGGGCCAGGAGAGTGATGACCAC | 60.1 | 0.02 |
| mm3_intergenic_Gm13291|Gm13292_chr2_11387134_F | TCGTCGGCAGCGTCAGTGAGCTGCAGTGGTCATC | 60.0 | 0.01 |
| mm3_intergenic_Gm13291|Gm13292_chr2_11387134_R | GTCTCGTGGGCTCGGGCCAGGAGAGTGATGACCAC | 60.1 | 0.01 |
| mm4_intergenic_Gm13364|Gm6587_chr2_15811656_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13364|Gm6587_chr2_15811656_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_4930563E18Rik|Mib1_chr18_10719966_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_4930563E18Rik|Mib1_chr18_10719966_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Fgf9|Gm25614_chr14_58477818_F | TCGTCGGCAGCGTCCCTGTCAGGCACTCCACAAT | 59.9 | 0.01 |
| mm4_intergenic_Fgf9|Gm25614_chr14_58477818_R | GTCTCGTGGGCTCGGAAGCTTGTATGGGCCTCGTC | 60.1 | 0.01 |
| mm4_intergenic_Tmem254c|RP23-93L3.3_chr14_26077057_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tmem254c|RP23-93L3.3_chr14_26077057_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tmem254b|RP23-100J17.8_chr14_26216672_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tmem254b|RP23-100J17.8_chr14_26216672_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tmem254a|Cphx1_chr14_25937304_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tmem254a|Cphx1_chr14_25937304_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Skap2_chr6_51898892_F | TCGTCGGCAGCGTCGCTGACCCATCTGCACAAAC | 59.7 | 0.01 |
| mm4_intron_Skap2_chr6_51898892_R | GTCTCGTGGGCTCGGGTGCACACAGCTACACATGC | 60.1 | 0.01 |
| mm4_intergenic_Gm22797|Robo1_chr16_72045679_F | TCGTCGGCAGCGTCCAGCTCCTCTGGTTGTGTCC | 60.3 | 0.00 |
| mm4_intergenic_Gm22797|Robo1_chr16_72045679_R | GTCTCGTGGGCTCGGACACCACTGTGACCATGGTT | 59.4 | 0.00 |
| mm3_intergenic_Gm24383|Kcnc4_chr3_107421199_F | TCGTCGGCAGCGTCTGAGTCTCATGGAAAGATTTGGT | 57.7 | 0.00 |
| mm3_intergenic_Gm24383|Kcnc4_chr3_107421199_R | GTCTCGTGGGCTCGGCCTCTCTTATCAACTGCTCCCC | 59.8 | 0.00 |
| mm4_intron_Dpyd_chr3_119079261_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dpyd_chr3_119079261_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Polm|Aebp1_chr11_5844443_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Polm|Aebp1_chr11_5844443_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23821|Tgfb2_chr1_186568455_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23821|Tgfb2_chr1_186568455_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22547|Gm8919_chr3_11601339_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22547|Gm8919_chr3_11601339_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_BC016548|Elf5_chr2_103406089_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_BC016548|Elf5_chr2_103406089_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313572 |
TCACTGTGGCAGACGTTTCTATTTATCCACTTGCGTTCGCCGAGTGGCGTCACCAGCGGTACTGTAATGACGATTGCAGC AGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGC |
| mm3_intergenic_Gm22126|Cdyl_chr13_35669786 |
CAGAATGCTGGCTGGCTTTCGGATTCTGTAAGGACCTGAGAAAGTACCTAAAGTGCCTTGGTTCCCCATCTACTGTAATA AATTCCTCCTGTTGCAATTGTCATTGCAGGTGGTGACCAGAGAACAGAGGC |
| mm4_intergenic_Gm12636|Gm12632_chr4_90752652 |
TAACCTAGGTCCAGGCCTGTTTTAAGTGTGGACAGTAGTGATGGAGAAGTTTGGCAGTAAGGACGTTTACAGCAGAGGGT GACCTTGTGTGCACTACCC |
| mm4_intergenic_Gm13575|Gm13577_chr2_64408270 |
GGTGTAAGGAAAGCTGCCACAGCTGTCTCTATTACTATTATGCCACCTGCTGCTATCACCATTGCCCCTGTTATGTCTCT ATTGCTGCATTCCTCTTGTTATATTTAGGGTGGTAGTCTGACCACCATCTGCTTTTGTGC |
| mm4_intergenic_Gm25258|Hao1_chr2_134076800 |
GTAACCTCCGACCTTTGCCAGCTAACATAAACTAATTATGTTTGCAGCAGGAGGGGAGAGAGGCTGTGGGTAGTCTTCCG TGG |
| mm4_intron_4930563I02Rik_chr14_60089959 |
TGAGAAGGGCCAGCTTTGAGCTGGGTTTCATTTGAAGCCACAGAAGATGTCTAAGCAAGAAAGAGGAACACCCAGCAGTA ATGGGGAAAGCAGCAGGAGGTTGGCTATACAGACAGCTGTGCAGTCTAGACACA |
| mm4_intron_Cept1_chr3_106546921 |
TGAACACATTCCTGACAGTTGTAAAGTAGAAAGGAAATATTCTAACAACTTACAAAGTTCCTATACTCGTTGAAAACTGA CTATGAATTTGCCTCCTGCTGCAATAGACGTTGCCTGGTGAGTTAGAGTTTTAGCAATGC |
| mm4_intron_Ccdc132_chr6_3538122 | AAATGGACAGCCTGTGTGGTGTGCACCACAGCCTCCAGCTGCTATCGTCTTTTCTTTAAGTGCCTTCATAGTGCCACCT |
| mm4_intron_Prickle1_chr15_93512315 |
CCTATCAGAAACGCCTGTGGTGCCCTGCATTTGCATTAATCACGATGGCAGAAGGAGGGCATTACTGAAACCAAACCAGA CCTTAGACAGCAAAGCCAGCCAGGGAGATGAGAGGTTCCACACTTCCCTGG |
| mm4_intergenic_Sstr5|Sox8_chr17_25537569 |
GCTCCCTGCTTCTGATGGTAACCTAAGGCTAACTGGGATCTCTCTCCCACATTGGGAAAAACATCGTCTGGGTAGCCTCC TGCTGCCATAGTCACTGCTCCTGGGAAGAGTCCTGACCACT |
| mm4_intergenic_9130015L21Rik|Gm826_chr2_160233630 |
AAGAGCCTTTGCCTCATCCCTGCCCTCCTATGACACAATCCACCTGCTGCAGTCTGCATCACTACTGTCTCCGCCCTGCC CAACGCCAACACCACCTCAGGATCC |
| mm4_intron_Fabp3_chr4_130313452 |
TCCACTGGGATGTCTGGACAATGGGGAATGAAGATTCCAGAAGGTGGAATATAGACAGGAAAGAGATGAGTGACAGCCAT CACCTTTCTAGACTCCTACCGACAAACCCTGCTT |
| mm4_intergenic_Olfr420|Spta1_chr1_174170615 |
CCTGAAAGGGACCATGTCACAAACATGCTGTCCTTACAAGGCTTCTGAAGATTGATCTGCCTACTGCTGCCATCCACATT ACTGTACTGAGAAACAATCATTGGTGGATGAGGGCAG |
| mm4_intergenic_Gm12075|Gm12077_chr11_27486412 |
AGCAGCTAGTGACAATCTGCATCAAGTCTCTCATCATCTCAGGAGACTGACCGGTAAAGACCACTCCAGCAGGCGGAGGG ATGGATGAGAAGAAGTGAAGCAGCAGCATCCAT |
| mm4_intron_Gm10532_chr18_75517030 |
GGCCTCTGTTCTTGACTGGGAGAAAAGAGAGGGATGTTTCTGGTTCCCTCCTCTTGCAATCGCCATTGCTCCATGACTGT GACCTGTCAGTACTGGTACTGAGCAGACAGCCAGACACAGAT |
| mm3_intron_Nsmce2_chr15_59448193 |
ACTCCTGTGATCTCCCCTCCCAGTGCGATGTTGCCTGTAAGGCAGTAATGATAATTGCAGGAGGAGGAGATCTTGACTTT GCCCAGTGG |
| mm4_intron_Galnt18_chr7_111749706 |
TGCAAGGAAATGGACTGGCTTTCTAGATAATGCCTGAGCCATCCATCCTTCCTGCTGCAAGCCTCACTTCACAGGGCCAA AAGGTTCCAA |
| mm3_intergenic_5430402E10Rik|Gm14750_chrX_77953978 |
CAGCTCCTCTGGTTGTGTCCAGACTGCTTGATCCCCTGCTGCAATCCTCAATAACCCCTGCTGCTACAGTCTCTTCTCAG AAAAAGAAAGTCATTCTTAATCTTCCCTTCATAGTCAACCATGGTCACAGTGGTGT |
| mm4_intergenic_Ankrd9|Rcor1_chr12_111013006 |
ACTGTTGTTGGCCCAGAGAATGGTAAGGATGAATGCAGGAGGTGGGCTTCTGTGAGGCAGTGAGGACAGAGAGCATGTTC TGTGGTGTGCAG |
| mm4_intergenic_Vma21|Gm1141_chrX_71893301 |
GGCTCTCCCCATCTTCAAGGCTGACTTAATTTAACTTTTCTGGTGATAAGGAGAAGCATGCAGAATATTTTCCCTTCTCT GTTATCTCCTGCTGCAATCCTCATAGTCAAATACAAAATGGAAACGTCCCATAGATCAGT |
| mm3_exon_Gm13291_chr2_11386724 |
ACCTGGCTGCAGTGATCATCACTCTCCTGGCTGCAGTGAGCTGCAATGGTCATCACTCTCCTGCTGCAATGATCATCACT CTTCTGGCTGCAGTGAACTGCAGTGGTCATCACTCTCCTGGC |
| mm3_intergenic_Gm13291|Gm13292_chr2_11387134 |
AGTGAGCTGCAGTGGTCATCACTCTCCTGCTGCAATGTTCATCACTCTCCTGGCTGCAGTGAGCTGCAGTGGTCATCACC CTCCTGGCTGCAGTGAGCTGCAGTGGTCATCACTCTCCTGGC |
| mm4_intergenic_Fgf9|Gm25614_chr14_58477818 |
CCTGTCAGGCACTCCACAATTCCTCTGGCCCAGGCTGTCATCAGCACTCTCCTGCTGCAATCATCTCTAGTCCTCAGGAC TTGACGAGGCCCATACAAGCTT |
| mm4_intron_Skap2_chr6_51898892 |
GCTGACCCATCTGCACAAACAGTGAAGGCTGATACTCTCCTGCTGCAAACGTCTCCACAGATGCATGTGTAGCTGTGTGC AC |
| mm4_intergenic_Gm22797|Robo1_chr16_72045679 |
CAGCTCCTCTGGTTGTGTCCCAACTGCTTGATCCCCTGCTGCAATCCTCTGTAACCCCTGCTGCTACAGTCTCTTCTCAG AAAAAGAAAGTCATTCTCAATCTTCCCTTCATAGTCAACCATGGTCACAGTGGTGT |
| mm3_intergenic_Gm24383|Kcnc4_chr3_107421199 | TGAGTCTCATGGAAAGATTTGGTTGATTGGTAAAGAGGATTTCAGCAGGGGAGGGGAGCAGTTGATAAGAGAGG |