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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAAT | (Wild-type sequence) | |
| 362 | CCGAAAGGACATGGTTATGTTTCTGGATGG--GTCAGCTTGGCACTCTGGTTGGTAAGAG | 2 bp deleted → frameshift |
| 344 | CCGAAAGGACATGGTTATGTTTCTGGATGG---TCAGCTTGGCACTCTGGTTGGTAAGAG | 3 bp deleted → no frameshift |
| 296 | CCGAAAGGACATGGTTATGTTTCTGG------GTCAGCTTGGCACTCTGGTTGGTAAGAG | 6 bp deleted → no frameshift |
| 282 | CCGAAAGGACATGGTTATGTTTCTGG-------TCAGCTTGGCACTCTGGTTGGTAAGAG | 7 bp deleted → frameshift |
| 274 | CCGAAAGGACATGGTTATGTTTCTGGATGG------------CACTCTGGTTGGTAAGAG | 12 bp deleted → no frameshift |
| 240 | CCGAAAGGACATGGTTATGTTTCTGG------------------------TTGGTAAGAG | 24 bp deleted → no frameshift |
| 224 | CCGAAAGGACATGGTTATGTTTCTGG----------------CACTCTGGTTGGTAAGAG | 16 bp deleted → frameshift |
| 193 | CCGAAAGGACATGGT-------------------CAGCTTGGCACTCTGGTTGGTAAGAG | 19 bp deleted → frameshift |
| 184 | CCGAAAGGACATGGTTATGTTTCTGGATGG--------------------TTGGTAAGAG | 20 bp deleted → frameshift |
| 164 | CCGAAAGGACATGGTTATGTTTC------------AGCTTGGCACTCTGGTTGGTAAGAG | 12 bp deleted → no frameshift |
| 162 | CCGAAAGGACATGG------------------GTCAGCTTGGCACTCTGGTTGGTAAGAG | 18 bp deleted → no frameshift |
| 150 | CCGAAAGGACATGGTTATGTTTCTGGATGG------------------------TAAGAG | 24 bp deleted → no frameshift |
| 149 | CCGAAAGGACATGGTTATGT--------------CAGCTTGGCACTCTGGTTGGTAAGAG | 14 bp deleted → frameshift |
| 141 | CCGAAAGGACATGGTTATGTTTCT---------------TGGCACTCTGGTTGGTAAGAG | 15 bp deleted → no frameshift |
| 123 | CCGAAAGGACATGGTTATGTTTCTGG----------------------------TAAGAG | 28 bp deleted → frameshift |
| 123 | CCGAAAGGACATGG----------------------------CACTCTGGTTGGTAAGAG | 28 bp deleted → frameshift |
| 120 | CCGAAAGG------------------------GTCAGCTTGGCACTCTGGTTGGTAAGAG | 24 bp deleted → no frameshift |
| 115 | CCGAAAGGACATGGTT------------------------------------GGTAAGAG | 36 bp deleted → no frameshift |
| 114 | CCGAAAGG-------------------------TCAGCTTGGCACTCTGGTTGGTAAGAG | 25 bp deleted → frameshift |
| 99 | CCGAAAGGACATGGTTATGTTTCT----------------------CTGGTTGGTAAGAG | 22 bp deleted → frameshift |
| 99 | CCGAAAGGACATGGTTATG----------------------GCACTCTGGTTGGTAAGAG | 22 bp deleted → frameshift |
| 86 | CCGAAAGGACA-------------------------GCTTGGCACTCTGGTTGGTAAGAG | 25 bp deleted → frameshift |
| 84 | CCGAAAGGACATGGTTATGTT-------------------------------GGTAAGAG | 31 bp deleted → frameshift |
| 81 | CCGAAAGGACATGGTTATGTTT------------------GGCACTCTGGTTGGTAAGAG | 18 bp deleted → no frameshift |
| 81 | CCGAAAGGACATGGT----------------------------------------AAGAG | 40 bp deleted → frameshift |
| 77 | CCGAAAGGACATGGTTATGTT-------------------GGCACTCTGGTTGGTAAGAG | 19 bp deleted → frameshift |
| 73 | CCGAAAGG----------------------------------CACTCTGGTTGGTAAGAG | 34 bp deleted → frameshift |
| 66 | CCGAAAGGACATGGTTATG------------------------------GTTGGTAAGAG | 30 bp deleted → no frameshift |
| 66 | CCGAAAG------------------------------CTTGGCACTCTGGTTGGTAAGAG | 30 bp deleted → no frameshift |
| 60 | CCGAAAGGACATGGTTATGTTTCTGGA--------------------------------G | 32 bp deleted → frameshift |
| 60 | CCGAAAGGACATGGTT------------------------GGCACTCTGGTTGGTAAGAG | 24 bp deleted → no frameshift |
| 57 | CCGAAAGGACA---------------------------------CTCTGGTTGGTAAGAG | 33 bp deleted → no frameshift |
| 54 | CCGAAAGGACATGGTTATG----------------------------------GTAAGAG | 34 bp deleted → frameshift |
| 52 | CCGAAAGGACATGGTTATGT-----------------------------------AAGAG | 35 bp deleted → frameshift |
| 52 | CCGAAAGGAC-----------------------------------TCTGGTTGGTAAGAG | 35 bp deleted → frameshift |
| 48 | CCGAAAGG------------------------------------------TTGGTAAGAG | 42 bp deleted → no frameshift |
| 44 | CCGAAAGGACATGGTTATGTTT------------------------------GGTAAGAG | 30 bp deleted → no frameshift |
| 40 | CCGAAAGG----------------------------------------------TAAGAG | 46 bp deleted → frameshift |
| 31 | CCGAAAG---------------------------------------------------AG | 51 bp deleted → no frameshift |
| 28 | CCGAAAGGACATGGTTA---------------------------------------AGAG | 39 bp deleted → no frameshift |
| 24 | CCGAAAGGA--------------------------------------------------G | 50 bp deleted → frameshift |
| 21 | CCGAAAG----------------------------------------------------- | 53 bp deleted → frameshift |
| 19 | CCGA-------------------------------------------------------G | 55 bp deleted → frameshift |
| 14 | CCGAA----------------------------------------------------GAG | 52 bp deleted → frameshift |