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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313926_F | TCGTCGGCAGCGTCGCTTGGCACTCTGGTTGGTA | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313926_R | GTCTCGTGGGCTCGGCACCATCTTCTCTGGCGGTT | 60.0 | 1.00 |
| mm4_intergenic_Plod2|Gm9621_chr9_92755779_F | TCGTCGGCAGCGTCCGCCAAGTCCAGTAGCAAGA | 60.0 | 0.69 |
| mm4_intergenic_Plod2|Gm9621_chr9_92755779_R | GTCTCGTGGGCTCGGCCCCAGGTCTAGAGAGTCAT | 57.2 | 0.69 |
| mm4_intergenic_Foxl1|5033426O07Rik_chr8_121177826_F | TCGTCGGCAGCGTCTGTACCACTGAGGGTCACCA | 60.1 | 0.61 |
| mm4_intergenic_Foxl1|5033426O07Rik_chr8_121177826_R | GTCTCGTGGGCTCGGGCTTTCCACCTGAGATGCCT | 60.0 | 0.61 |
| mm4_intergenic_AI606473|Tyw3_chr3_154335181_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_AI606473|Tyw3_chr3_154335181_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm3_intron_Asb18_chr1_89998751_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm3_intron_Asb18_chr1_89998751_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm3_intergenic_Gm10309|Prkce_chr17_86526163_F | TCGTCGGCAGCGTCTGCCACCAAGACTCAAGCTC | 60.2 | 0.49 |
| mm3_intergenic_Gm10309|Prkce_chr17_86526163_R | GTCTCGTGGGCTCGGGACGGCTCGGCAAACAAAC | 60.3 | 0.49 |
| mm4_intron_Bach2_chr4_32426948_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Bach2_chr4_32426948_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Gm22191|Gm22726_chr14_40231873_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm22191|Gm22726_chr14_40231873_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm24465|B230216N24Rik_chr1_98050475_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm24465|B230216N24Rik_chr1_98050475_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Clip2_chr5_134489655_F | TCGTCGGCAGCGTCCTTCTGGTCCCTTCCGTGG | 59.7 | 0.25 |
| mm4_exon_Clip2_chr5_134489655_R | GTCTCGTGGGCTCGGACAGCAGCCTAGAACTGACG | 59.7 | 0.25 |
| mm4_exon_Eri3_chr4_117564772_F | TCGTCGGCAGCGTCGTTCCGGAGCTCTGTTCCAT | 59.7 | 0.17 |
| mm4_exon_Eri3_chr4_117564772_R | GTCTCGTGGGCTCGGGGCCTCAAAGTCCAGCACTA | 59.6 | 0.17 |
| mm4_intron_Sorcs3_chr19_48577412_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Sorcs3_chr19_48577412_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Acot11/Fam151a_chr4_106747833_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Acot11/Fam151a_chr4_106747833_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Rbfox1|Gm23476_chr16_7672603_F | TCGTCGGCAGCGTCGCTGAGTGAAATTTCCAGCGT | 59.4 | 0.13 |
| mm4_intergenic_Rbfox1|Gm23476_chr16_7672603_R | GTCTCGTGGGCTCGGCTGTCTCAGGTGCAGCATGT | 60.3 | 0.13 |
| mm4_intergenic_Gm23185|Gm23848_chr15_49060700_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm23185|Gm23848_chr15_49060700_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Pcbp3_chr10_76770794_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Pcbp3_chr10_76770794_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ccnd2|9330179D12Rik_chr6_127181683_F | TCGTCGGCAGCGTCGGAGGTGGCCAGAGCTTTG | 60.6 | 0.07 |
| mm4_intergenic_Ccnd2|9330179D12Rik_chr6_127181683_R | GTCTCGTGGGCTCGGTGTCCAAACCTCATCCAGCC | 59.9 | 0.07 |
| mm4_intergenic_Epn1|Rfpl4_chr7_5109490_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Epn1|Rfpl4_chr7_5109490_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm2_intergenic_Fbxw7|Mir466k_chr3_85323895_F | TCGTCGGCAGCGTCCGTTTTGTAGCCAAAGCCCC | 60.0 | 0.06 |
| mm2_intergenic_Fbxw7|Mir466k_chr3_85323895_R | GTCTCGTGGGCTCGGCAGAAAGCATCATTTCCAGACAA | 57.6 | 0.06 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36532822_F | TCGTCGGCAGCGTCCGATGCCAAACACCTTTCTGA | 59.1 | 0.06 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36532822_R | GTCTCGTGGGCTCGGCAGCAAGAAATCCATACGCGT | 59.3 | 0.06 |
| mm3_intergenic_9230110C19Rik|AK129341_chr9_8046903_F | TCGTCGGCAGCGTCGAGCGATGTCTCTCTCTGGC | 59.9 | 0.05 |
| mm3_intergenic_9230110C19Rik|AK129341_chr9_8046903_R | GTCTCGTGGGCTCGGCAGAGACGGGGTAAATGCCA | 59.7 | 0.05 |
| mm4_intergenic_BC094916|Pydc4_chr1_173562629_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_BC094916|Pydc4_chr1_173562629_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Plekhg1_chr10_3746785_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Plekhg1_chr10_3746785_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ppp1r13b|2010107E04Rik_chr12_111940553_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ppp1r13b|2010107E04Rik_chr12_111940553_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Mroh1_chr15_76410220_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Mroh1_chr15_76410220_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm16120_chr5_140210379_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm16120_chr5_140210379_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Nfasc_chr1_132580726_F | TCGTCGGCAGCGTCTTCTCCGCACATACGCTGTC | 60.4 | 0.03 |
| mm4_intron_Nfasc_chr1_132580726_R | GTCTCGTGGGCTCGGAGGCCTTCCCATTTCCCATG | 60.0 | 0.03 |
| mm4_intergenic_Gm25148|Tifab_chr13_56172095_F | TCGTCGGCAGCGTCATGCCCTAAACCTGGGTTCC | 59.6 | 0.03 |
| mm4_intergenic_Gm25148|Tifab_chr13_56172095_R | GTCTCGTGGGCTCGGAGTTGTCCCTCCCTCTCCTG | 60.2 | 0.03 |
| mm4_intergenic_Rnase6|Rnase1_chr14_51131905_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rnase6|Rnase1_chr14_51131905_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Col6a6|Col6a5_chr9_105828125_F | TCGTCGGCAGCGTCCGCAGATGAATGCTGCTCTC | 59.4 | 0.02 |
| mm4_intergenic_Col6a6|Col6a5_chr9_105828125_R | GTCTCGTGGGCTCGGCACGTTGCAGAGAAACAGGC | 60.0 | 0.02 |
| mm4_intron_Vwa3a_chr7_120774342_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Vwa3a_chr7_120774342_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24856|Car10_chr11_93448630_F | TCGTCGGCAGCGTCTTGGGTTCTCATAACGGGCC | 60.0 | 0.02 |
| mm4_intergenic_Gm24856|Car10_chr11_93448630_R | GTCTCGTGGGCTCGGTTGCGGGGATTTCTGTGTGT | 60.1 | 0.02 |
| mm4_intron_Gm20388/Galnt2_chr8_124340706_F | TCGTCGGCAGCGTCTTACACCTTAGCAGCCAGGC | 60.0 | 0.02 |
| mm4_intron_Gm20388/Galnt2_chr8_124340706_R | GTCTCGTGGGCTCGGTGCAGTCACGTGGGAAACTT | 60.1 | 0.02 |
| mm4_intron_Lsm14b_chr2_180031893_F | TCGTCGGCAGCGTCCACACTCAAGGGCAGGTGAA | 60.1 | 0.02 |
| mm4_intron_Lsm14b_chr2_180031893_R | GTCTCGTGGGCTCGGACCTTAGTCCCAAGTCCCCA | 59.8 | 0.02 |
| mm3_intergenic_Zmiz1|Ppif_chr14_25675718_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Zmiz1|Ppif_chr14_25675718_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Odf2_chr2_29893127_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Odf2_chr2_29893127_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ptpla_chr2_14055101_F | TCGTCGGCAGCGTCTGAAGACACTGCCCAGCAAT | 59.8 | 0.01 |
| mm4_intron_Ptpla_chr2_14055101_R | GTCTCGTGGGCTCGGGCCTGCTGTTGAAGAAACGG | 60.0 | 0.01 |
| mm4_intergenic_mmu-mir-7689|Sorcs2_chr5_36304472_F | TCGTCGGCAGCGTCGCCCATGTGGTAGCATCCAA | 60.3 | 0.01 |
| mm4_intergenic_mmu-mir-7689|Sorcs2_chr5_36304472_R | GTCTCGTGGGCTCGGAGCTTTCTGAGTCTGTTGCCA | 59.8 | 0.01 |
| mm4_intergenic_Fam120b|Psmb1_chr17_15458226_F | TCGTCGGCAGCGTCGAAGGGATCAGTGTGCTCCC | 60.1 | 0.01 |
| mm4_intergenic_Fam120b|Psmb1_chr17_15458226_R | GTCTCGTGGGCTCGGATGCCTGCCCAGCTAATAGC | 60.2 | 0.01 |
| mm4_intergenic_Trrap|Smurf1_chr5_144870793_F | TCGTCGGCAGCGTCAGACTGAATCCAGCCTTGGG | 59.3 | 0.01 |
| mm4_intergenic_Trrap|Smurf1_chr5_144870793_R | GTCTCGTGGGCTCGGCTGGATGGATAGGGGCCATG | 59.6 | 0.01 |
| mm4_intergenic_Gm10742|Mir124a-2_chr3_17793794_F | TCGTCGGCAGCGTCGCCTTCTGCAGCTCTTTGGT | 60.8 | 0.01 |
| mm4_intergenic_Gm10742|Mir124a-2_chr3_17793794_R | GTCTCGTGGGCTCGGAAGAGGATTACACGGGCTGC | 60.1 | 0.01 |
| mm4_exon_Araf_chrX_20859748_F | TCGTCGGCAGCGTCGTCTCCGGACCTCAGCAAAA | 59.9 | 0.01 |
| mm4_exon_Araf_chrX_20859748_R | GTCTCGTGGGCTCGGGTATCCAGCCCTCCTACCCA | 60.1 | 0.01 |
| mm4_intergenic_Slc7a9|Tdrd12_chr7_35490881_F | TCGTCGGCAGCGTCTGTTTCAGAAGTCTGTGACCA | 57.0 | 0.00 |
| mm4_intergenic_Slc7a9|Tdrd12_chr7_35490881_R | GTCTCGTGGGCTCGGGAACAAGCTGCGACGACATG | 60.1 | 0.00 |
| mm4_intergenic_Mir326|Arrb1_chr7_99571020_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mir326|Arrb1_chr7_99571020_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Lztfl1/Gm17200_chr9_123700844_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Lztfl1/Gm17200_chr9_123700844_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Crebbp_chr16_4157098_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Crebbp_chr16_4157098_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Clmn_chr12_104863686_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Clmn_chr12_104863686_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Wfs1|Jakmip1_chr5_37015704_F | TCGTCGGCAGCGTCGTGGGAGTGCCATGAGACAA | 59.9 | 0.00 |
| mm4_intergenic_Wfs1|Jakmip1_chr5_37015704_R | GTCTCGTGGGCTCGGGGACAAGGCGGGTTCGATAT | 59.8 | 0.00 |
| mm4_intron_Lmo7_chr14_101914538_F | TCGTCGGCAGCGTCAATTGTGGCCAGGTGAGAGG | 59.9 | 0.00 |
| mm4_intron_Lmo7_chr14_101914538_R | GTCTCGTGGGCTCGGCTGGCCCTGGGAAAATCTGT | 59.9 | 0.00 |
| mm4_exon_2900005J15Rik_chr5_25101464_F | TCGTCGGCAGCGTCGTGCTCCCGGATCTTCAGAG | 59.8 | 0.00 |
| mm4_exon_2900005J15Rik_chr5_25101464_R | GTCTCGTGGGCTCGGCTGAGCAGGAGAGGAAGCAC | 60.1 | 0.00 |
| mm4_intergenic_Olfr1292-ps1|Olfr1293-ps_chr2_111517381_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr1292-ps1|Olfr1293-ps_chr2_111517381_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_M1ap_chr6_82949035_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_M1ap_chr6_82949035_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fgr_chr4_132994789_F | TCGTCGGCAGCGTCTCATTCGGGAAAGCGAGACC | 60.1 | 0.00 |
| mm4_intron_Fgr_chr4_132994789_R | GTCTCGTGGGCTCGGGAATTCCCAGAGCCCAGCTT | 60.0 | 0.00 |
| mm4_intron_Elfn2_chr15_78686939_F | TCGTCGGCAGCGTCGAGACAAAGGCTCCCCTGAC | 60.0 | 0.00 |
| mm4_intron_Elfn2_chr15_78686939_R | GTCTCGTGGGCTCGGCTCTGACCCTGGGCTCTTTC | 59.7 | 0.00 |
| mm4_intron_Cpne5_chr17_29228975_F | TCGTCGGCAGCGTCAGAAGCCTGCACCACTGTTT | 60.1 | 0.00 |
| mm4_intron_Cpne5_chr17_29228975_R | GTCTCGTGGGCTCGGGGAAGGACCCCAAGAACCTG | 59.9 | 0.00 |
| mm4_intergenic_Susd5|4921528I07Rik_chr9_114235338_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Susd5|4921528I07Rik_chr9_114235338_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Susd5|4921528I07Rik_chr9_114193022_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Susd5|4921528I07Rik_chr9_114193022_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pcdh17|Gm23926_chr14_85260951_F | TCGTCGGCAGCGTCGGTACCCAGCATGAAGTGACA | 59.9 | 0.00 |
| mm4_intergenic_Pcdh17|Gm23926_chr14_85260951_R | GTCTCGTGGGCTCGGTGGTAGCTTGATTATATCTTTCGCA | 58.4 | 0.00 |
| mm4_intergenic_Gm16583|Far2_chr6_148073217_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16583|Far2_chr6_148073217_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_E330017L17Rik|Spocd1_chr4_129936422_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_E330017L17Rik|Spocd1_chr4_129936422_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313926 |
GCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGA TGGTG |
| mm4_intergenic_Plod2|Gm9621_chr9_92755779 |
CGCCAAGTCCAGTAGCAAGACCCAAAGCTGTGAGCAGCCTGCCTTACAGAGTGTCTTCACTGATCCTATGGAATATGACT CTCTAGACCTGGGG |
| mm4_intergenic_Foxl1|5033426O07Rik_chr8_121177826 |
TGTACCACTGAGGGTCACCAGGTTGTTAGTTCCCTCAGGCTCCCCAACATCTTTCATTCCCCAAAGCCGTGAGTAGCTGT CTGCGACCACACTAGGCATCTCAGGTGGAAAGC |
| mm3_intergenic_Gm10309|Prkce_chr17_86526163 |
TGCCACCAAGACTCAAGCTCCGGTAATTTCTTCTGGGCTCTTTGTGATTGTAGAGTTTGTGAAATTTAAGTCAACACCCT TCTCTAATCCCCAAGCCGTGAGAAGCCCTCAGAGGGTTTGTTTGCCGAGCCGTC |
| mm4_exon_Clip2_chr5_134489655 |
CTTCTGGTCCCTTCCGTGGGAGCCTTTGGCTGTTAATTCCTAATCCGTGAGCTGCCCGATTGTGCTGGAAAGAGCAAGAT TTGGCTCTGCAGACGGGCCACTAAAGCGTCAGTTCTAGGCTGCTGT |
| mm4_exon_Eri3_chr4_117564772 |
GTTCCGGAGCTCTGTTCCATATCCACCAGAAAGCTGGCGGCCCACGGCTTTGGCGCGGCAATGGCGGCAATGGTGCCCTT CCCTCCCCAGAGGTATCACTACTTTTTAGTGCTGGACTTTGAGGCC |
| mm4_intergenic_Rbfox1|Gm23476_chr16_7672603 |
GCTGAGTGAAATTTCCAGCGTTTACCTCATGTTACCTTCTGCCAGCTGTCTGCACACAGCTTGGGTTGGGTTCTTATACA TGCTGCACCTGAGACAG |
| mm4_intergenic_Ccnd2|9330179D12Rik_chr6_127181683 |
GGAGGTGGCCAGAGCTTTGCTCCAGCCAGCCTGCTCTGGGCCTAGGGGCAGCCTCCTGCTCCCGGCTTAGGCAGTTCGTG GGGGGCTGGATGAGGTTTGGACA |
| mm2_intergenic_Fbxw7|Mir466k_chr3_85323895 |
CGTTTTGTAGCCAAAGCCCCACAGAAAGCAATCAGAAACATTTAAGGCTGGGATTTCTGAAGCCGAGAGCAGCCCCCTGT GTGCTTCTTAGAGCATTGATTAGATTTGTCTGGAAATGATGCTTTCTG |
| mm4_intergenic_Gm25247|Tshz3_chr7_36532822 |
CGATGCCAAACACCTTTCTGAGAGGGAGAAAAGAAAATAATGAAAATAATCAGGTGAGTTCACTTCATTCTTCCTGCAAC CTAAAAATAAACGCAAGGGGCTTCTCCCGGCTTCGGGGAACGCGTATGGATTTCTTGCTG |
| mm3_intergenic_9230110C19Rik|AK129341_chr9_8046903 |
GAGCGATGTCTCTCTCTGGCTTAGGAGGCCTTTTAAAAAGCGACTGTAATGGAAAGAGGGCTGCTCATGGCTTGGGAGGG AACGGTAAGGACTTTCAGAAATGGGTTGGCATTTACCCCGTCTCTG |
| mm4_intron_Nfasc_chr1_132580726 |
TTCTCCGCACATACGCTGTCTCATCCTGCGGTCCCTCTGAGCTCCCAAGCTCTGAGAAGCCTGCTGCTTGAGTTCTGCTT CTGCCCGCCCCTCATCCCAGCAGTGGAGCATGGGAAATGGGAAGGCCT |
| mm4_intergenic_Gm25148|Tifab_chr13_56172095 |
ATGCCCTAAACCTGGGTTCCACTTAAGTTGGGGTACAAAGGCTCAAGGAGGAAGAAAAAGGAAACACTGCCAAGTTCTCC AAAGCCCTGGGCTGCCCACTGAGCAGGAGAGGGAGGGACAACT |
| mm4_intergenic_Col6a6|Col6a5_chr9_105828125 |
CGCAGATGAATGCTGCTCTCCACGCCCTGGTGGCCCCCAGGGCTCCGCGTGCTGCTCAAGCCTTGGGAAGGTGACAGAAG AGGGAAGGGGGGCGGATTTTGGAGGCTGGCACTATCCCGCCTGTTTCTCTGCAACGTG |
| mm4_intergenic_Gm24856|Car10_chr11_93448630 |
TTGGGTTCTCATAACGGGCCACACAAAGGGAAAAAAAAGATGGAACACCATAGGACCTAAATGAAATAACCCAAGCCATG AGCATCACTCTGTGTGCCTCATGACACACACAGAAATCCCCGCAA |
| mm4_intron_Gm20388/Galnt2_chr8_124340706 |
TTACACCTTAGCAGCCAGGCGTAGCTTTGTCCCTCAGCCGGGAGCATCCTGCTGGCTCTGAGTTACCCCTGGGGCCTGAG TCAAGTTTCCCACGTGACTGCA |
| mm4_intron_Lsm14b_chr2_180031893 |
CACACTCAAGGGCAGGTGAATGATGAGAACAGAAGACCTCCACGGAGGCGGTCAGGTAACGCCCATGGCCACTGAGTCCA TAGCCATGAGCAGCAGGCTGTGCTTTGCTTGCCTGTGCTGGGGACTTGGGACTAAGGT |
| mm4_intron_Ptpla_chr2_14055101 |
TGAAGACACTGCCCAGCAATCCACAGCACCAGGAACCACACGGCCAGGGTCCGGTGCCCACAGCCAGCGGACAGCTCCCG GCTCGGGTGAATAGACCGGGCCGTTTCTTCAACAGCAGGC |
| mm4_intergenic_mmu-mir-7689|Sorcs2_chr5_36304472 |
GCCCATGTGGTAGCATCCAAATGTGTGGATGTTTGTATTTCCCAATCCCTGAGCAGCCGTCTGGTGAATGTCAGCTGTTG CTCCAGCCATCTGGCAACAGACTCAGAAAGCT |
| mm4_intergenic_Fam120b|Psmb1_chr17_15458226 |
GAAGGGATCAGTGTGCTCCCCAGACCATAATGGTATTCAGCTGGCTTCTGGCGGCTTAGGGGGCTATTAGCTGGGCAGGC AT |
| mm4_intergenic_Trrap|Smurf1_chr5_144870793 |
AGACTGAATCCAGCCTTGGGCTCACAGAGCTAAGCTGTAGAGGAGCCATCAGCGGCCAGCTGACGGCTGAGGCACCAGGG ACCAGCAGCCATGGCCCCTATCCATCCAG |
| mm4_intergenic_Gm10742|Mir124a-2_chr3_17793794 |
GCCTTCTGCAGCTCTTTGGTTTCAAGGACACAGCGAGCTGTTCAGGACTTTGGGAGCCAACATCTGTCTGACTGGTGACG TCAGCAGCCCGTGTAATCCTCTT |
| mm4_exon_Araf_chrX_20859748 |
GTCTCCGGACCTCAGCAAAATCTTCAGTAATTGCCCCAAAGCCATGAGGCGCCTGCTGACTGACTGCCTCAAGTTCCAGC GGGAGGAGCGGCCCCTATTTCCCCAGGTGGGCTGGGTAGGAGGGCTGGATAC |
| mm4_intergenic_Slc7a9|Tdrd12_chr7_35490881 |
TGTTTCAGAAGTCTGTGACCAAAAGTACAATTTCCCAAAATGATACTGAATTTTCTATGCTAACCTCCTTCCCAAAGCCC TGAGCTTCCAGCTGAGCTGAGGGGCCATGTCGTCGCAGCTTGTTC |
| mm4_intergenic_Wfs1|Jakmip1_chr5_37015704 |
GTGGGAGTGCCATGAGACAATTGAGCATTGCGCATTGCTGCCCTCCCCAAGCCCTGACCAGCCCAGTGGCCTGGCAGCAG GCCATATCGAACCCGCCTTGTCC |
| mm4_intron_Lmo7_chr14_101914538 |
AATTGTGGCCAGGTGAGAGGGAGTCAGTGCCAGCCTGGAATGTGTCAAGATCTCTAAACAAGCTCAGCAGCCTGCTCAGG GCTCTGGGTACCTAAGCCAAGGGAGAACAGATTTTCCCAGGGCCAG |
| mm4_exon_2900005J15Rik_chr5_25101464 |
GTGCTCCCGGATCTTCAGAGCCACTGGCTGCAGATCTGGTACTGCGGGCTGCTCAGGGCCCTGGTGTTCCTCCCAGGCAG CAGGACCTTGGCTTCTGGTCTGAGTGAGGGGCGTGCTTCCTCTCCTGCTCAG |
| mm4_intron_Fgr_chr4_132994789 |
TCATTCGGGAAAGCGAGACCACCAAAGGTGGGTTGGTACGGCAGACATGCCACCAGACAATAAGGACAGTGGGCTGGTCA GTGCTTGGGTCAGGGCCAAGTGGAGGGGCAGAGCTAAGCTGGGCTCTGGGAATTC |
| mm4_intron_Elfn2_chr15_78686939 |
GAGACAAAGGCTCCCCTGACCTCGTTGCTCTCTCACAGTGGAAGGTGACAAGGCTGCGGCCTGCTCACTGCCTAGGAGTG CGGCGCCACAGTGAAAGAGCCCAGGGTCAGAG |
| mm4_intron_Cpne5_chr17_29228975 |
AGAAGCCTGCACCACTGTTTAGCAACGGGCTGCTCACTCCCTGGGGCTCATGTCAGTTCCCACGGGTAAAAAAGGCAGGT TCTTGGGGTCCTTCC |
| mm4_intergenic_Pcdh17|Gm23926_chr14_85260951 |
GGTACCCAGCATGAAGTGACAACTCGAATGTCAGAGTTTGTTGGTAGGCAAAGGGCTGCTCACTGGTTAGGGAGATATGA TGATGCGAAAGATATAATCAAGCTACCA |