CRISPOR

DNA break repair outcome predictions

Guide: ACTCTCCAGAGGGGGGGAGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCTG`	(Wild-type sequence)
518	`CCATCCCCGGACTCTCCAGAGGGGGGGA------AAACCCCCAAGTAAGCTCCTAATCCC`	`6 bp deleted → no frameshift`
423	`CCATCCCCGGACTCTCCAGAGGGGGG-------AAAACCCCCAAGTAAGCTCCTAATCCC`	`7 bp deleted → frameshift`
402	`CCATCCCCGGACTCTCCAGAGGGGG--------AAAACCCCCAAGTAAGCTCCTAATCCC`	`8 bp deleted → frameshift`
382	`CCATCCCCGGACTCTCCAGAGGGG---------AAAACCCCCAAGTAAGCTCCTAATCCC`	`9 bp deleted → no frameshift`
364	`CCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCCTAATCCC`	`10 bp deleted → frameshift`
362	`CCATCCCCGGACTCTCCAGAGGGGGGGAGG--GAAAACCCCCAAGTAAGCTCCTAATCCC`	`2 bp deleted → frameshift`
344	`CCATCCCCGGACTCTCCAGAGGGGGGGAGG---AAAACCCCCAAGTAAGCTCCTAATCCC`	`3 bp deleted → no frameshift`
311	`CCATCCCCGGACTCTCCAGAGGGGGGG-----GAAAACCCCCAAGTAAGCTCCTAATCCC`	`5 bp deleted → frameshift`
230	`CCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCCTAATCCC`	`11 bp deleted → frameshift`
158	`CCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCCTAATCCC`	`23 bp deleted → frameshift`
153	`CCATCCCC---------------------------------CAAGTAAGCTCCTAATCCC`	`33 bp deleted → no frameshift`
149	`CCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCCTAATCCC`	`14 bp deleted → frameshift`
146	`CCATCCCC----------------------------------AAGTAAGCTCCTAATCCC`	`34 bp deleted → frameshift`
143	`CCATCCCCGGACTCTCCA-------------------------AGTAAGCTCCTAATCCC`	`25 bp deleted → frameshift`
134	`CCATCCCCGGACTCTCCAGAGGGGGGGAG----------------TAAGCTCCTAATCCC`	`16 bp deleted → frameshift`
133	`CCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCCTAATCCC`	`22 bp deleted → frameshift`
133	`CCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCCTAATCCC`	`22 bp deleted → frameshift`
126	`CCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCCTAATCCC`	`23 bp deleted → frameshift`
121	`CCATCCCC--------------------------------CCAAGTAAGCTCCTAATCCC`	`32 bp deleted → frameshift`
120	`CCATCCCCGGACTCTCC------------------------CAAGTAAGCTCCTAATCCC`	`24 bp deleted → no frameshift`
115	`CCATCCCCGGACTCTCC------------------------------------TAATCCC`	`36 bp deleted → no frameshift`
110	`CCATCCCCGGACTCTCCAGAGGGGGGGAG--------------------CTCCTAATCCC`	`20 bp deleted → frameshift`
104	`CCATCCC-----------------------------------AAGTAAGCTCCTAATCCC`	`35 bp deleted → frameshift`
90	`CCATCCCCGGACTCTCCAGAG------------------------TAAGCTCCTAATCCC`	`24 bp deleted → no frameshift`
84	`CCATCCCC-------------------------------CCCAAGTAAGCTCCTAATCCC`	`31 bp deleted → frameshift`
81	`CCATCCCCGGACTCTCCAG--------------------------TAAGCTCCTAATCCC`	`26 bp deleted → frameshift`
81	`CCATCCCCGGAC--------------------------CCCCAAGTAAGCTCCTAATCCC`	`26 bp deleted → frameshift`
75	`CCATCCCCGGACTC--------------------------------------CTAATCCC`	`38 bp deleted → frameshift`
74	`CCATCCCCGGACTCTCCAGAG----------------------------CTCCTAATCCC`	`28 bp deleted → frameshift`
67	`CCA----------------------------------------AGTAAGCTCCTAATCCC`	`40 bp deleted → frameshift`
66	`CCATCCCCGGACTCTCCAG------------------------------CTCCTAATCCC`	`30 bp deleted → no frameshift`
66	`CCATCC------------------------------------AAGTAAGCTCCTAATCCC`	`36 bp deleted → no frameshift`
62	`CC-------------------------------------CCCAAGTAAGCTCCTAATCCC`	`37 bp deleted → frameshift`
61	`CCATCCCCGGACTCTCC------------------------------------------C`	`42 bp deleted → no frameshift`
60	`CC--------------------------------------CCAAGTAAGCTCCTAATCCC`	`38 bp deleted → frameshift`
56	`CCATCCC-----------------------------------------------------`	`53 bp deleted → frameshift`
56	`CC---------------------------------------CAAGTAAGCTCCTAATCCC`	`39 bp deleted → no frameshift`
47	`CCATCC-----------------------------------------------TAATCCC`	`47 bp deleted → frameshift`
46	`CCATCCCCGGACTCTCC-------------------------------------------`	`43 bp deleted → frameshift`
44	`CCATCCCC----------------------------------------------------`	`52 bp deleted → frameshift`
42	`CCATCCCCGGACTCT---------------------------------------AATCCC`	`39 bp deleted → no frameshift`
42	`CCATCCCC---------------------------------------------TAATCCC`	`45 bp deleted → no frameshift`
40	`CCATCCC----------------------------------------------TAATCCC`	`46 bp deleted → frameshift`
38	`CCATCCCCGGACT-----------------------------------------AATCCC`	`41 bp deleted → frameshift`
33	`CCATCCCCGGACTC--------------------------------------------CC`	`44 bp deleted → frameshift`
31	`CCATCCCC---------------------------------------------------C`	`51 bp deleted → no frameshift`
31	`CC---------------------------------------------------TAATCCC`	`51 bp deleted → no frameshift`
26	`CCATCC------------------------------------------------------`	`54 bp deleted → no frameshift`
23	`CC---------------------------------------------------------C`	`57 bp deleted → no frameshift`
22	`CC----------------------------------------------------------`	`58 bp deleted → frameshift`

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