CRISPOR

DNA break repair outcome predictions

Guide: GTTTTCCCCCTCCCCCCCTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTAT`	(Wild-type sequence)
219	`TTACTTGGGGGTTTTCCCCCTCCCCCCC------------GGGGATGGAGAGCCGAAAGG`	`12 bp deleted → no frameshift`
213	`TTACTTGGGGGTTTTCCCCCTCC-----------------GGGGATGGAGAGCCGAAAGG`	`17 bp deleted → frameshift`
208	`TTACTTGGGGGTTTTCCCCCTCCCCCC-------------GGGGATGGAGAGCCGAAAGG`	`13 bp deleted → frameshift`
198	`TTACTTGGGGGTTTTCCCCCTCCCCC--------------GGGGATGGAGAGCCGAAAGG`	`14 bp deleted → frameshift`
191	`TTACTTGGGGGTTTTCCCCCTCCCCCCCTC---------CGGGGATGGAGAGCCGAAAGG`	`9 bp deleted → no frameshift`
188	`TTACTTGGGGGTTTTCCCCCTCCCC---------------GGGGATGGAGAGCCGAAAGG`	`15 bp deleted → no frameshift`
179	`TTACTTGGGGGTTTTCCCCCTCCC----------------GGGGATGGAGAGCCGAAAGG`	`16 bp deleted → frameshift`
158	`TTACTTGGGGGTTTTCC-----------------------GGGGATGGAGAGCCGAAAGG`	`23 bp deleted → frameshift`
153	`TTACTTGGGGG---------------------------------ATGGAGAGCCGAAAGG`	`33 bp deleted → no frameshift`
147	`TTACTTGGGGGTTTTCCCCC--------------------GGGGATGGAGAGCCGAAAGG`	`20 bp deleted → frameshift`
146	`TTACTTGGGG----------------------------------ATGGAGAGCCGAAAGG`	`34 bp deleted → frameshift`
143	`TTACTTGG-------------------------AGAGTCCGGGGATGGAGAGCCGAAAGG`	`25 bp deleted → frameshift`
140	`TTACTTGGGGGTTTTCCCC---------------------GGGGATGGAGAGCCGAAAGG`	`21 bp deleted → no frameshift`
133	`TTACTTGGGGGTTTTCCC----------------------GGGGATGGAGAGCCGAAAGG`	`22 bp deleted → frameshift`
133	`TTACTTGGGGG----------------------AGAGTCCGGGGATGGAGAGCCGAAAGG`	`22 bp deleted → frameshift`
126	`TTACTTGGGG-----------------------AGAGTCCGGGGATGGAGAGCCGAAAGG`	`23 bp deleted → frameshift`
121	`TTACTTGGGGG--------------------------------GATGGAGAGCCGAAAGG`	`32 bp deleted → frameshift`
120	`TTACTTGGG------------------------AGAGTCCGGGGATGGAGAGCCGAAAGG`	`24 bp deleted → no frameshift`
109	`TTACTTGGGGGTTTTCCCCCTCCCCCCC--------------------------GAAAGG`	`26 bp deleted → frameshift`
104	`TTACTTGGG-----------------------------------ATGGAGAGCCGAAAGG`	`35 bp deleted → frameshift`
103	`TTACTTGGGGGTTTTCCCCCTCCCCCC---------------------------GAAAGG`	`27 bp deleted → no frameshift`
98	`TTACTTGGGGGTTTTCCCCCTCCCCC----------------------------GAAAGG`	`28 bp deleted → frameshift`
94	`TTACTTGGGGGTTTTCCCCCTCCCC-----------------------------GAAAGG`	`29 bp deleted → frameshift`
89	`TTACTTGGGGGTTTTCCCCCTCCC------------------------------GAAAGG`	`30 bp deleted → no frameshift`
84	`TTACTTGGGGGTTTTCCCCCTCC-------------------------------GAAAGG`	`31 bp deleted → frameshift`
84	`TTACTTGGGGG-------------------------------GGATGGAGAGCCGAAAGG`	`31 bp deleted → frameshift`
81	`TTACTTGGGGGT--------------------------CCGGGGATGGAGAGCCGAAAGG`	`26 bp deleted → frameshift`
73	`TTACTTGGGGGTTTTCCCCC----------------------------------GAAAGG`	`34 bp deleted → frameshift`
69	`TTACTTGGGGGTTTTCCCC-----------------------------------GAAAGG`	`35 bp deleted → frameshift`
67	`TTACTTGG----------------------------------------AGAGCCGAAAGG`	`40 bp deleted → frameshift`
66	`TTACTTGGGGGTTTTCCC------------------------------------GAAAGG`	`36 bp deleted → no frameshift`
66	`TTACTTGG------------------------------------ATGGAGAGCCGAAAGG`	`36 bp deleted → no frameshift`
62	`TTACTTGGGGGTTTTCC-------------------------------------GAAAGG`	`37 bp deleted → frameshift`
62	`TTACTTGGGGG-------------------------------------AGAGCCGAAAGG`	`37 bp deleted → frameshift`
60	`TTACTTGGGG--------------------------------------AGAGCCGAAAGG`	`38 bp deleted → frameshift`
56	`TTACTTGGG---------------------------------------AGAGCCGAAAGG`	`39 bp deleted → no frameshift`
34	`TTACTTGGGGG-------------------------------------------------`	`49 bp deleted → frameshift`
32	`TTACTTGGGG--------------------------------------------------`	`50 bp deleted → frameshift`
31	`TTACTTGGG---------------------------------------------------`	`51 bp deleted → no frameshift`
29	`TTACTTGG----------------------------------------------------`	`52 bp deleted → frameshift`

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